Genetic transformation of sweet oranges to over-express SABP2 gene
Identifieur interne : 000A00 ( Pmc/Corpus ); précédent : 000999; suivant : 000A01Genetic transformation of sweet oranges to over-express SABP2 gene
Auteurs : Lísia Borges Attílio ; Polyana Kelly Martins ; Laura Melissa G Mez-Krapp ; Marcos Antônio Machado ; Juliana Freitas-AstúaSource :
- BMC Proceedings [ 1753-6561 ] ; 2014.
Url:
DOI: 10.1186/1753-6561-8-S4-P109
PubMed: NONE
PubMed Central: 4210677
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic transformation of sweet oranges to over-express <italic>SABP2 </italic>gene</title><author><name sortKey="Attilio, Lisia Borges" sort="Attilio, Lisia Borges" uniqKey="Attilio L" first="Lísia Borges" last="Attílio">Lísia Borges Attílio</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Martins, Polyana Kelly" sort="Martins, Polyana Kelly" uniqKey="Martins P" first="Polyana Kelly" last="Martins">Polyana Kelly Martins</name><affiliation><nlm:aff id="I2">EMBRAPA Agroenergy - Estação Parque Biológico, Brasilia, DF, Brazil</nlm:aff></affiliation></author><author><name sortKey="G Mez Krapp, Laura Melissa" sort="G Mez Krapp, Laura Melissa" uniqKey="G Mez Krapp L" first="Laura Melissa" last="G Mez-Krapp">Laura Melissa G Mez-Krapp</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antônio" last="Machado">Marcos Antônio Machado</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Freitas Astua, Juliana" sort="Freitas Astua, Juliana" uniqKey="Freitas Astua J" first="Juliana" last="Freitas-Astúa">Juliana Freitas-Astúa</name><affiliation><nlm:aff id="I3">EMBRAPA Cassava & Fruits, Cruz das Almas, Bahia, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">4210677</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210677</idno><idno type="RBID">PMC:4210677</idno><idno type="doi">10.1186/1753-6561-8-S4-P109</idno><idno type="pmid">NONE</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000A00</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genetic transformation of sweet oranges to over-express <italic>SABP2 </italic>gene</title><author><name sortKey="Attilio, Lisia Borges" sort="Attilio, Lisia Borges" uniqKey="Attilio L" first="Lísia Borges" last="Attílio">Lísia Borges Attílio</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Martins, Polyana Kelly" sort="Martins, Polyana Kelly" uniqKey="Martins P" first="Polyana Kelly" last="Martins">Polyana Kelly Martins</name><affiliation><nlm:aff id="I2">EMBRAPA Agroenergy - Estação Parque Biológico, Brasilia, DF, Brazil</nlm:aff></affiliation></author><author><name sortKey="G Mez Krapp, Laura Melissa" sort="G Mez Krapp, Laura Melissa" uniqKey="G Mez Krapp L" first="Laura Melissa" last="G Mez-Krapp">Laura Melissa G Mez-Krapp</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antônio" last="Machado">Marcos Antônio Machado</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Freitas Astua, Juliana" sort="Freitas Astua, Juliana" uniqKey="Freitas Astua J" first="Juliana" last="Freitas-Astúa">Juliana Freitas-Astúa</name><affiliation><nlm:aff id="I3">EMBRAPA Cassava & Fruits, Cruz das Almas, Bahia, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author></analytic><series><title level="j">BMC Proceedings</title><idno type="eISSN">1753-6561</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Forouhar, F" uniqKey="Forouhar F">F Forouhar</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name></author><author><name sortKey="Kumar, D" uniqKey="Kumar D">D Kumar</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Fridman, E" uniqKey="Fridman E">E Fridman</name></author><author><name sortKey="Chiang, Y" uniqKey="Chiang Y">Y Chiang</name></author><author><name sortKey="Acton, Tb" uniqKey="Acton T">TB Acton</name></author><author><name sortKey="Montelione, Gt" uniqKey="Montelione G">GT Montelione</name></author><author><name sortKey="Pichersky, E" uniqKey="Pichersky E">E Pichersky</name></author><author><name sortKey="Klessig, Df" uniqKey="Klessig D">DF Klessig</name></author><author><name sortKey="Tong, L" uniqKey="Tong L">L Tong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miyata, Ly1" uniqKey="Miyata L">LY1 Miyata</name></author><author><name sortKey="Harakava, R" uniqKey="Harakava R">R Harakava</name></author><author><name sortKey="Stipp, Lc" uniqKey="Stipp L">LC Stipp</name></author><author><name sortKey="Mendes, Bm" uniqKey="Mendes B">BM Mendes</name></author><author><name sortKey="Appezzato Da Gl Ria, B" uniqKey="Appezzato Da Gl Ria B">B Appezzato-da-Glória</name></author><author><name sortKey="De Assis Alves Mourao Filho, F" uniqKey="De Assis Alves Mourao Filho F">F de Assis Alves Mourão Filho</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="abstract" xml:lang="en"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Proc</journal-id><journal-id journal-id-type="iso-abbrev">BMC Proc</journal-id><journal-title-group><journal-title>BMC Proceedings</journal-title></journal-title-group><issn pub-type="epub">1753-6561</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">4210677</article-id><article-id pub-id-type="publisher-id">1753-6561-8-S4-P109</article-id><article-id pub-id-type="doi">10.1186/1753-6561-8-S4-P109</article-id><article-categories><subj-group subj-group-type="heading"><subject>Poster Presentation</subject></subj-group></article-categories><title-group><article-title>Genetic transformation of sweet oranges to over-express <italic>SABP2 </italic>gene</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes" id="A1"><name><surname>Attílio</surname><given-names>Lísia Borges</given-names></name><xref ref-type="aff" rid="I1">1</xref></contrib><contrib contrib-type="author" id="A2"><name><surname>Martins</surname><given-names>Polyana Kelly</given-names></name><xref ref-type="aff" rid="I2">2</xref></contrib><contrib contrib-type="author" id="A3"><name><surname>Gómez-Krapp</surname><given-names>Laura Melissa</given-names></name><xref ref-type="aff" rid="I1">1</xref></contrib><contrib contrib-type="author" id="A4"><name><surname>Machado</surname><given-names>Marcos Antônio</given-names></name><xref ref-type="aff" rid="I1">1</xref></contrib><contrib contrib-type="author" id="A5"><name><surname>Freitas-Astúa</surname><given-names>Juliana</given-names></name><xref ref-type="aff" rid="I3">3</xref></contrib></contrib-group><aff id="I1"><label>1</label>Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</aff><aff id="I2"><label>2</label>EMBRAPA Agroenergy - Estação Parque Biológico, Brasilia, DF, Brazil</aff><aff id="I3"><label>3</label>EMBRAPA Cassava & Fruits, Cruz das Almas, Bahia, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</aff><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>1</day><month>10</month><year>2014</year></pub-date><volume>8</volume><issue>Suppl 4</issue><supplement><named-content content-type="supplement-title">The 5th Congress of the Brazilian Biotechnology Society (SBBIOTEC): Meeting abstracts</named-content><named-content content-type="supplement-editor">Luiz Antônio Barreto de Castro and Dario Grattapaglia</named-content><named-content content-type="supplement-sponsor">The publication charges for this supplement were funded by the Federal District Research Foundation Brazil - (Fundação de Amparo á Pesquisa do Distrito Federal (FAP-DF)-Brasil).</named-content></supplement><fpage>P109</fpage><lpage>P109</lpage><permissions><copyright-statement>Copyright © 2014 Attílio et al.; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Attílio et al.; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><self-uri xlink:href="http://www.biomedcentral.com/1753-6561/8/S4/P109"></self-uri><conference><conf-date>10-14 November 2013</conf-date><conf-name>5th Congress of the Brazilian Biotechnology Society (SBBIOTEC)</conf-name><conf-loc>Florianópolis, Brazil</conf-loc></conference></article-meta></front><body><sec><title>Background</title><p>The history of the world citrus industry is marked by a series of diseases caused by different etiologic agents. Several of them are characterized as biotrophic pathogens like <italic>Xanthomonas citri </italic>subsp. <italic>citri</italic>, three <italic>Candidatus </italic>Liberibacter spp and <italic>Citrus leprosis virus C </italic>(CiLV-C). In plant-pathogen interactions, the role of salicylic acid (SA) in activating defense related genes is well recognized [<xref ref-type="bibr" rid="B1">1</xref>]. The SABP2 (Salicylic acid-binding protein 2) is required to convert methyl salicylate to SA as part of the signal transduction pathway that activates systemic acquired resistance, induces PR expression, and enhances disease resistance. Due to the reduced or absence of genetic resistance to these pathogens in commercial sweet orange cultivars, the genetic transformation to over-express a gene involved in the defense response of plants is a possible alternative to produce tolerant or resistant plants to biotrophic pathogens.</p></sec><sec sec-type="methods"><title>Methods</title><p>The aim of this study was to produce 'Hamlin' sweet orange (<italic>Citrus sinensis </italic>L. Osb.) transgenic plants, via <italic>Agrobacterium tumefaciens</italic>, over-expressing the <italic>SABP2 </italic>gene from sweet orange driven by the constitutive promoter ubiquitin (<italic>Ubq10</italic>). The <italic>SABP2 </italic>gene was cloned into pCambia 2301 and inserted into <italic>A. tumefaciens </italic>EHA 105. The genetic transformation was performed using epicotyl segments from seedlings [<xref ref-type="bibr" rid="B2">2</xref>].</p></sec><sec><title>Results and conclusions</title><p>A total of 620 explants in three independent experiments were introduced and approximately 336 shoots were regenerated. The GUS histochemical test was performed and confirmed the transformation of 30 positive shoots. These shoots are being grafted onto Carrizo citrange [<italic>Citrus sinensis </italic>x <italic>Poncirus trifoliata </italic>(L.) Raf.] seedlings grown in test tubes containing MS culture medium. The presence of the transgene will be evaluated by PCR using specifics primers that amplify part of the ubiquitin promoter and part of the gene. <italic>SABP2 </italic>expression levels in transgenic plants will be assessed through qPCR. After bud multiplication, transgenic plants will be evaluated for their response to citrus canker, HLB and leprosis.</p></sec></body><back><sec><title>Acknowledgements</title><p>INCT-Citrus (Fapesp and CNPq), Embrapa-Capes grant and EMBRAPA-Monsanto agreement (02.08.05.004.00.00).</p></sec><ref-list><ref id="B1"><mixed-citation publication-type="journal"><name><surname>Forouhar</surname><given-names>F</given-names></name><name><surname>Yang</surname><given-names>Y</given-names></name><name><surname>Kumar</surname><given-names>D</given-names></name><name><surname>Chen</surname><given-names>Y</given-names></name><name><surname>Fridman</surname><given-names>E</given-names></name><name><surname>Chiang</surname><given-names>Y</given-names></name><name><surname>Acton</surname><given-names>TB</given-names></name><name><surname>Montelione</surname><given-names>GT</given-names></name><name><surname>Pichersky</surname><given-names>E</given-names></name><name><surname>Klessig</surname><given-names>DF</given-names></name><name><surname>Tong</surname><given-names>L</given-names></name><article-title>Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity</article-title><source>Proceedings of the National Academy of Sciences</source><year>2005</year><volume>102</volume><issue>5</issue><fpage>1773</fpage><lpage>1778</lpage><pub-id pub-id-type="doi">10.1073/pnas.0409227102</pub-id><pub-id pub-id-type="pmid">15668381</pub-id></mixed-citation></ref><ref id="B2"><mixed-citation publication-type="journal"><name><surname>Miyata</surname><given-names>LY1</given-names></name><name><surname>Harakava</surname><given-names>R</given-names></name><name><surname>Stipp</surname><given-names>LC</given-names></name><name><surname>Mendes</surname><given-names>BM</given-names></name><name><surname>Appezzato-da-Glória</surname><given-names>B</given-names></name><name><surname>de Assis Alves Mourão Filho</surname><given-names>F</given-names></name><article-title>GUS expression in sweet oranges (Citrus sinensis L. Osbeck) driven by three different phloem-specific promoters</article-title><source>Plant Cell Report</source><year>2012</year><volume>31</volume><issue>11</issue><fpage>2005</fpage><lpage>2013</lpage><pub-id pub-id-type="doi">10.1007/s00299-012-1312-2</pub-id><pub-id pub-id-type="pmid">22801867</pub-id></mixed-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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