Selection and evaluation of citrus phloem specific promoters
Identifieur interne : 000999 ( Pmc/Corpus ); précédent : 000998; suivant : 000A00Selection and evaluation of citrus phloem specific promoters
Auteurs : Laura Melissa G Mez-Krapp ; Polyana Kelly Martins ; Francisco De Assis Alves Mourão Filho ; Liliane Cristina Liborio Stipp ; Lísia Borges Attílio ; Rodrigo Guarino Cassarotti ; Marcos Antônio Machado ; Juliana Freitas-AstúaSource :
- BMC Proceedings [ 1753-6561 ] ; 2014.
Url:
DOI: 10.1186/1753-6561-8-S4-P99
PubMed: NONE
PubMed Central: 4204348
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selection and evaluation of citrus phloem specific promoters</title><author><name sortKey="G Mez Krapp, Laura Melissa" sort="G Mez Krapp, Laura Melissa" uniqKey="G Mez Krapp L" first="Laura Melissa" last="G Mez-Krapp">Laura Melissa G Mez-Krapp</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Martins, Polyana Kelly" sort="Martins, Polyana Kelly" uniqKey="Martins P" first="Polyana Kelly" last="Martins">Polyana Kelly Martins</name><affiliation><nlm:aff id="I2">EMBRAPA Agroenergy - Estação Parque Biológico, Brasilia, DF, Brazil</nlm:aff></affiliation></author><author><name sortKey="Mourao Filho, Francisco De Assis Alves" sort="Mourao Filho, Francisco De Assis Alves" uniqKey="Mourao Filho F" first="Francisco De Assis Alves" last="Mourão Filho">Francisco De Assis Alves Mourão Filho</name><affiliation><nlm:aff id="I3">Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Liborio Stipp, Liliane Cristina" sort="Liborio Stipp, Liliane Cristina" uniqKey="Liborio Stipp L" first="Liliane Cristina" last="Liborio Stipp">Liliane Cristina Liborio Stipp</name><affiliation><nlm:aff id="I3">Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Attilio, Lisia Borges" sort="Attilio, Lisia Borges" uniqKey="Attilio L" first="Lísia Borges" last="Attílio">Lísia Borges Attílio</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Cassarotti, Rodrigo Guarino" sort="Cassarotti, Rodrigo Guarino" uniqKey="Cassarotti R" first="Rodrigo Guarino" last="Cassarotti">Rodrigo Guarino Cassarotti</name><affiliation><nlm:aff id="I4">Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antônio" last="Machado">Marcos Antônio Machado</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Freitas Astua, Juliana" sort="Freitas Astua, Juliana" uniqKey="Freitas Astua J" first="Juliana" last="Freitas-Astúa">Juliana Freitas-Astúa</name><affiliation><nlm:aff id="I5">EMBRAPA Cassava & Fruits, Cruz das Almas, Bahia, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">4204348</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204348</idno><idno type="RBID">PMC:4204348</idno><idno type="doi">10.1186/1753-6561-8-S4-P99</idno><idno type="pmid">NONE</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000999</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Selection and evaluation of citrus phloem specific promoters</title><author><name sortKey="G Mez Krapp, Laura Melissa" sort="G Mez Krapp, Laura Melissa" uniqKey="G Mez Krapp L" first="Laura Melissa" last="G Mez-Krapp">Laura Melissa G Mez-Krapp</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Martins, Polyana Kelly" sort="Martins, Polyana Kelly" uniqKey="Martins P" first="Polyana Kelly" last="Martins">Polyana Kelly Martins</name><affiliation><nlm:aff id="I2">EMBRAPA Agroenergy - Estação Parque Biológico, Brasilia, DF, Brazil</nlm:aff></affiliation></author><author><name sortKey="Mourao Filho, Francisco De Assis Alves" sort="Mourao Filho, Francisco De Assis Alves" uniqKey="Mourao Filho F" first="Francisco De Assis Alves" last="Mourão Filho">Francisco De Assis Alves Mourão Filho</name><affiliation><nlm:aff id="I3">Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Liborio Stipp, Liliane Cristina" sort="Liborio Stipp, Liliane Cristina" uniqKey="Liborio Stipp L" first="Liliane Cristina" last="Liborio Stipp">Liliane Cristina Liborio Stipp</name><affiliation><nlm:aff id="I3">Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Attilio, Lisia Borges" sort="Attilio, Lisia Borges" uniqKey="Attilio L" first="Lísia Borges" last="Attílio">Lísia Borges Attílio</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Cassarotti, Rodrigo Guarino" sort="Cassarotti, Rodrigo Guarino" uniqKey="Cassarotti R" first="Rodrigo Guarino" last="Cassarotti">Rodrigo Guarino Cassarotti</name><affiliation><nlm:aff id="I4">Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antônio" last="Machado">Marcos Antônio Machado</name><affiliation><nlm:aff id="I1">Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author><author><name sortKey="Freitas Astua, Juliana" sort="Freitas Astua, Juliana" uniqKey="Freitas Astua J" first="Juliana" last="Freitas-Astúa">Juliana Freitas-Astúa</name><affiliation><nlm:aff id="I5">EMBRAPA Cassava & Fruits, Cruz das Almas, Bahia, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</nlm:aff></affiliation></author></analytic><series><title level="j">BMC Proceedings</title><idno type="eISSN">1753-6561</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Miyata, Ly" uniqKey="Miyata L">LY Miyata</name></author><author><name sortKey="Harakava, R" uniqKey="Harakava R">R Harakava</name></author><author><name sortKey="Stipp, Lcl" uniqKey="Stipp L">LCL Stipp</name></author><author><name sortKey="Mendes, Bm" uniqKey="Mendes B">BM Mendes</name></author><author><name sortKey="Appezzato Da Gl Ria, B" uniqKey="Appezzato Da Gl Ria B">B Appezzato-da-Glória</name></author><author><name sortKey="De Assis Alves Mourao Filho, F" uniqKey="De Assis Alves Mourao Filho F">F de Assis Alves Mourão Filho</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="abstract" xml:lang="en"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Proc</journal-id><journal-id journal-id-type="iso-abbrev">BMC Proc</journal-id><journal-title-group><journal-title>BMC Proceedings</journal-title></journal-title-group><issn pub-type="epub">1753-6561</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmc">4204348</article-id><article-id pub-id-type="publisher-id">1753-6561-8-S4-P99</article-id><article-id pub-id-type="doi">10.1186/1753-6561-8-S4-P99</article-id><article-categories><subj-group subj-group-type="heading"><subject>Poster Presentation</subject></subj-group></article-categories><title-group><article-title>Selection and evaluation of citrus phloem specific promoters</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes" id="A1"><name><surname>Gómez-Krapp</surname><given-names>Laura Melissa</given-names></name><xref ref-type="aff" rid="I1">1</xref></contrib><contrib contrib-type="author" id="A2"><name><surname>Martins</surname><given-names>Polyana Kelly</given-names></name><xref ref-type="aff" rid="I2">2</xref></contrib><contrib contrib-type="author" id="A3"><name><surname>Mourão Filho</surname><given-names>Francisco de Assis Alves</given-names></name><xref ref-type="aff" rid="I3">3</xref></contrib><contrib contrib-type="author" id="A4"><name><surname>Liborio Stipp</surname><given-names>Liliane Cristina</given-names></name><xref ref-type="aff" rid="I3">3</xref></contrib><contrib contrib-type="author" id="A5"><name><surname>Attílio</surname><given-names>Lísia Borges</given-names></name><xref ref-type="aff" rid="I1">1</xref></contrib><contrib contrib-type="author" id="A6"><name><surname>Cassarotti</surname><given-names>Rodrigo Guarino</given-names></name><xref ref-type="aff" rid="I4">4</xref></contrib><contrib contrib-type="author" id="A7"><name><surname>Machado</surname><given-names>Marcos Antônio</given-names></name><xref ref-type="aff" rid="I1">1</xref></contrib><contrib contrib-type="author" id="A8"><name><surname>Freitas-Astúa</surname><given-names>Juliana</given-names></name><xref ref-type="aff" rid="I5">5</xref></contrib></contrib-group><aff id="I1"><label>1</label>Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</aff><aff id="I2"><label>2</label>EMBRAPA Agroenergy - Estação Parque Biológico, Brasilia, DF, Brazil</aff><aff id="I3"><label>3</label>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil</aff><aff id="I4"><label>4</label>Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</aff><aff id="I5"><label>5</label>EMBRAPA Cassava & Fruits, Cruz das Almas, Bahia, Brazil Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, SP, Brazil</aff><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>1</day><month>10</month><year>2014</year></pub-date><volume>8</volume><issue>Suppl 4</issue><supplement><named-content content-type="supplement-title">The 5th Congress of the Brazilian Biotechnology Society (SBBIOTEC): Meeting abstracts</named-content><named-content content-type="supplement-editor">Luiz Antônio Barreto de Castro and Dario Grattapaglia</named-content><named-content content-type="supplement-sponsor">The publication charges for this supplement were funded by the Federal District Research Foundation Brazil - (Fundação de Amparo á Pesquisa do Distrito Federal (FAP-DF)-Brasil).</named-content></supplement><fpage>P99</fpage><lpage>P99</lpage><permissions><copyright-statement>Copyright © 2014 Gómez-Krapp et al.; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Gómez-Krapp et al.; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><self-uri xlink:href="http://www.biomedcentral.com/1753-6561/8/S4/P99"></self-uri><conference><conf-date>10-14 November 2013</conf-date><conf-name>5th Congress of the Brazilian Biotechnology Society (SBBIOTEC)</conf-name><conf-loc>Florianópolis, Brazil</conf-loc></conference></article-meta></front><body><sec><title>Background</title><p>The citrus industry worldwide has faced major economic losses due to the occurrence of <italic>Huanglongbing </italic>(HLB), a disease caused by <italic>Candidatus </italic>Liberibacter spp., Gram-negative bacteria restricted to the phloem of their hosts. Due to the lack of commercial varieties that could resist to these bacteria, genetic transformation comes as an important alternative for improving these varieties and managing the disease. Most of the work with citrus genetic transformation uses constitutive promoters, but recently studies have intensified the search for tissue-specific promoters, making possible to direct the transgene expression to the phloem and reduce it in other undesired parts of the plant, such as the fruits. It is possible that tissue-specific promoter sequences obtained from the same plant species have better acceptance by the customers and this was the objective of our work: prospect phloem-specific promoters from the citrus genome that can be used for citrus transformation with the objective to deliver transgene expression directly to the tissue of <italic>Ca</italic>. Liberibacter spp. accumulation.</p></sec><sec sec-type="methods"><title>Methods</title><p>Promoters prospection was made from available citrus EST databases (CitEST/HarvEST, available locally), and from the complete genome sequences of <italic>Citrus sinensis </italic>and <italic>C. clementina </italic>(available at <ext-link ext-link-type="uri" xlink:href="http://www.phytozome.net">http://www.phytozome.net</ext-link>). Five selected sequences, identified with the program PLACE (<ext-link ext-link-type="uri" xlink:href="http://www.dna.affrc.org.jp/place/">http://www.dna.affrc.org.jp/place/</ext-link>), were cloned into pCambia 2301 containing the <italic>uid</italic>A gene and inserted into <italic>Agrobacterium tumefaciens </italic>EHA 105. The genetic transformation was performed according to Miyata <italic>et al</italic>. [<xref ref-type="bibr" rid="B1">1</xref>].</p></sec><sec><title>Results and conclusions</title><p>Epicotyl segments obtained from Carrizo citrange and Hamlin sweet orange seedlings were used as source of explants for genetic transformation. From each promoter, three independent experiments were made. In each experiment an average of 300 explants were introduced and approximately 200 buds regenerated. The GUS histochemical test was performed and confirmed the transformation of 2 to 24% positive plants, depending on the construction. The preliminary results exhibit preferential expression in phloem cells. The transgene presence is being confirmed by PCR. A comparison among the GUS expression induced by the promoters will be conducted in order to determine which of them is more efficient in driving the transgene expression to the phloem cells. Such promoter will be further used in expression cassettes constructed with candidate genes for the control of HLB.</p></sec></body><back><sec><title>Acknowledgements</title><p>INCT-Citrus (Fapesp and CNPq), Embrapa-Capes grant and EMBRAPA-Monsanto agreement (02.08.05.004.00.00).</p></sec><ref-list><ref id="B1"><mixed-citation publication-type="journal"><name><surname>Miyata</surname><given-names>LY</given-names></name><name><surname>Harakava</surname><given-names>R</given-names></name><name><surname>Stipp</surname><given-names>LCL</given-names></name><name><surname>Mendes</surname><given-names>BM</given-names></name><name><surname>Appezzato-da-Glória</surname><given-names>B</given-names></name><name><surname>de Assis Alves Mourão Filho</surname><given-names>F</given-names></name><article-title>GUS expression in sweet oranges (Citrus sinensis L. Osbeck) driven by three different phloem-specific promoters</article-title><source>Plant Cell Report</source><year>2012</year><volume>31</volume><issue>11</issue><fpage>2005</fpage><lpage>2013</lpage></mixed-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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