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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>Citrus</italic> Chlorophyllase Dynamics at Ethylene-Induced Fruit Color-Break: A Study of Chlorophyllase Expression, Posttranslational Processing Kinetics, and in Situ Intracellular Localization<xref ref-type="fn" rid="fn1">1</xref><xref ref-type="fn" rid="fn2">[OA]</xref></title><author><name sortKey="Azoulay Shemer, Tamar" sort="Azoulay Shemer, Tamar" uniqKey="Azoulay Shemer T" first="Tamar" last="Azoulay Shemer">Tamar Azoulay Shemer</name></author><author><name sortKey="Harpaz Saad, Smadar" sort="Harpaz Saad, Smadar" uniqKey="Harpaz Saad S" first="Smadar" last="Harpaz-Saad">Smadar Harpaz-Saad</name></author><author><name sortKey="Belausov, Eduard" sort="Belausov, Eduard" uniqKey="Belausov E" first="Eduard" last="Belausov">Eduard Belausov</name></author><author><name sortKey="Lovat, Nicole" sort="Lovat, Nicole" uniqKey="Lovat N" first="Nicole" last="Lovat">Nicole Lovat</name></author><author><name sortKey="Krokhin, Oleg" sort="Krokhin, Oleg" uniqKey="Krokhin O" first="Oleg" last="Krokhin">Oleg Krokhin</name></author><author><name sortKey="Spicer, Victor" sort="Spicer, Victor" uniqKey="Spicer V" first="Victor" last="Spicer">Victor Spicer</name></author><author><name sortKey="Standing, Kenneth G" sort="Standing, Kenneth G" uniqKey="Standing K" first="Kenneth G." last="Standing">Kenneth G. Standing</name></author><author><name sortKey="Goldschmidt, Eliezer E" sort="Goldschmidt, Eliezer E" uniqKey="Goldschmidt E" first="Eliezer E." last="Goldschmidt">Eliezer E. Goldschmidt</name></author><author><name sortKey="Eyal, Yoram" sort="Eyal, Yoram" uniqKey="Eyal Y" first="Yoram" last="Eyal">Yoram Eyal</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18633118</idno><idno type="pmc">2528117</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528117</idno><idno type="RBID">PMC:2528117</idno><idno type="doi">10.1104/pp.108.124933</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000820</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>Citrus</italic> Chlorophyllase Dynamics at Ethylene-Induced Fruit Color-Break: A Study of Chlorophyllase Expression, Posttranslational Processing Kinetics, and in Situ Intracellular Localization<xref ref-type="fn" rid="fn1">1</xref><xref ref-type="fn" rid="fn2">[OA]</xref></title><author><name sortKey="Azoulay Shemer, Tamar" sort="Azoulay Shemer, Tamar" uniqKey="Azoulay Shemer T" first="Tamar" last="Azoulay Shemer">Tamar Azoulay Shemer</name></author><author><name sortKey="Harpaz Saad, Smadar" sort="Harpaz Saad, Smadar" uniqKey="Harpaz Saad S" first="Smadar" last="Harpaz-Saad">Smadar Harpaz-Saad</name></author><author><name sortKey="Belausov, Eduard" sort="Belausov, Eduard" uniqKey="Belausov E" first="Eduard" last="Belausov">Eduard Belausov</name></author><author><name sortKey="Lovat, Nicole" sort="Lovat, Nicole" uniqKey="Lovat N" first="Nicole" last="Lovat">Nicole Lovat</name></author><author><name sortKey="Krokhin, Oleg" sort="Krokhin, Oleg" uniqKey="Krokhin O" first="Oleg" last="Krokhin">Oleg Krokhin</name></author><author><name sortKey="Spicer, Victor" sort="Spicer, Victor" uniqKey="Spicer V" first="Victor" last="Spicer">Victor Spicer</name></author><author><name sortKey="Standing, Kenneth G" sort="Standing, Kenneth G" uniqKey="Standing K" first="Kenneth G." last="Standing">Kenneth G. Standing</name></author><author><name sortKey="Goldschmidt, Eliezer E" sort="Goldschmidt, Eliezer E" uniqKey="Goldschmidt E" first="Eliezer E." last="Goldschmidt">Eliezer E. Goldschmidt</name></author><author><name sortKey="Eyal, Yoram" sort="Eyal, Yoram" uniqKey="Eyal Y" first="Yoram" last="Eyal">Yoram Eyal</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Fruit color-break is the visual manifestation of the developmentally regulated transition of chloroplasts to chromoplasts during fruit ripening and often involves biosynthesis of copious amounts of carotenoids concomitant with massive breakdown of chlorophyll. Regulation of chlorophyll breakdown at different physiological and developmental stages of the plant life cycle, particularly at fruit color-break, is still not well understood. Here, we present the dynamics of native chlorophyllase (Chlase) and chlorophyll breakdown in lemon (<italic>Citrus limon</italic>) fruit during ethylene-induced color-break. We show, using in situ immunofluorescence on ethylene-treated fruit peel (flavedo) tissue, that citrus Chlase is located in the plastid, in contrast to recent reports suggesting cytoplasmic localization of Arabidopsis (<italic>Arabidopsis thaliana</italic>) Chlases. At the intra-organellar level, Chlase signal was found to overlap mostly with chlorophyll fluorescence, suggesting association of most of the Chlase protein with the photosynthetic membranes. Confocal microscopy analysis showed that the kinetics of chlorophyll breakdown was not uniform in the flavedo cells. Chlorophyll quantity at the cellular level was negatively correlated with plastid Chlase accumulation; plastids with reduced chlorophyll content were found by in situ immunofluorescence to contain significant levels of Chlase, while plastids containing still-intact chlorophyll lacked any Chlase signal. Immunoblot and protein-mass spectrometry analyses were used to demonstrate that citrus Chlase initially accumulates as an approximately 35-kD precursor, which is subsequently N-terminally processed to approximately 33-kD mature forms by cleavage at either of three consecutive amino acid positions. Chlase plastid localization, expression kinetics, and the negative correlation with chlorophyll levels support the central role of the enzyme in chlorophyll breakdown during citrus fruit color-break.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">plantphysiol</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18633118</article-id><article-id pub-id-type="pmc">2528117</article-id><article-id pub-id-type="publisher-id">124933</article-id><article-id pub-id-type="doi">10.1104/pp.108.124933</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Biochemical Processes and Macromolecular Structures</subject></subj-group></subj-group></article-categories><title-group><article-title><italic>Citrus</italic> Chlorophyllase Dynamics at Ethylene-Induced Fruit Color-Break: A Study of Chlorophyllase Expression, Posttranslational Processing Kinetics, and in Situ Intracellular Localization<xref ref-type="fn" rid="fn1">1</xref><xref ref-type="fn" rid="fn2">[OA]</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Azoulay Shemer</surname><given-names>Tamar</given-names></name></contrib><contrib contrib-type="author"><name><surname>Harpaz-Saad</surname><given-names>Smadar</given-names></name></contrib><contrib contrib-type="author"><name><surname>Belausov</surname><given-names>Eduard</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lovat</surname><given-names>Nicole</given-names></name></contrib><contrib contrib-type="author"><name><surname>Krokhin</surname><given-names>Oleg</given-names></name></contrib><contrib contrib-type="author"><name><surname>Spicer</surname><given-names>Victor</given-names></name></contrib><contrib contrib-type="author"><name><surname>Standing</surname><given-names>Kenneth G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Goldschmidt</surname><given-names>Eliezer E.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Eyal</surname><given-names>Yoram</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="N0x1c74730N0x1ed9700">Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel (T.A.S., S.H.-S., E.B., Y.E.); R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel (T.A.S., S.H.-S., E.E.G.); and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3P4 (N.L., O.K., V.S., K.G.S.)</aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author; e-mail <email>eyalab@volcani.agri.gov.il</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2008</year></pub-date><volume>148</volume><issue>1</issue><fpage>108</fpage><lpage>118</lpage><history><date date-type="received"><day>17</day><month>6</month><year>2008</year></date><date date-type="accepted"><day>10</day><month>7</month><year>2008</year></date></history><permissions><copyright-statement>Copyright © 2008, American Society of Plant Biologists</copyright-statement></permissions><abstract><p>Fruit color-break is the visual manifestation of the developmentally regulated transition of chloroplasts to chromoplasts during fruit ripening and often involves biosynthesis of copious amounts of carotenoids concomitant with massive breakdown of chlorophyll. Regulation of chlorophyll breakdown at different physiological and developmental stages of the plant life cycle, particularly at fruit color-break, is still not well understood. Here, we present the dynamics of native chlorophyllase (Chlase) and chlorophyll breakdown in lemon (<italic>Citrus limon</italic>) fruit during ethylene-induced color-break. We show, using in situ immunofluorescence on ethylene-treated fruit peel (flavedo) tissue, that citrus Chlase is located in the plastid, in contrast to recent reports suggesting cytoplasmic localization of Arabidopsis (<italic>Arabidopsis thaliana</italic>) Chlases. At the intra-organellar level, Chlase signal was found to overlap mostly with chlorophyll fluorescence, suggesting association of most of the Chlase protein with the photosynthetic membranes. Confocal microscopy analysis showed that the kinetics of chlorophyll breakdown was not uniform in the flavedo cells. Chlorophyll quantity at the cellular level was negatively correlated with plastid Chlase accumulation; plastids with reduced chlorophyll content were found by in situ immunofluorescence to contain significant levels of Chlase, while plastids containing still-intact chlorophyll lacked any Chlase signal. Immunoblot and protein-mass spectrometry analyses were used to demonstrate that citrus Chlase initially accumulates as an approximately 35-kD precursor, which is subsequently N-terminally processed to approximately 33-kD mature forms by cleavage at either of three consecutive amino acid positions. Chlase plastid localization, expression kinetics, and the negative correlation with chlorophyll levels support the central role of the enzyme in chlorophyll breakdown during citrus fruit color-break.</p></abstract></article-meta><notes><fn-group><fn id="fn1"><label>1</label><p>This work was supported by the Israel Science Foundation (grant no. 1291/04) and by a Levi Eshkol Ph.D. scholarship (to T.A.S.).</p></fn><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Yoram Eyal (<email>eyalab@volcani.agri.gov.il</email>).</p></fn><fn id="fn2"><label>[OA]</label><p>Open Access articles can be viewed online without a subscription.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org/cgi/doi/10.1104/pp.108.124933">www.plantphysiol.org/cgi/doi/10.1104/pp.108.124933</ext-link></p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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