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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the <italic>navel negra</italic> Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis<xref ref-type="fn" rid="fn1">1</xref><xref ref-type="fn" rid="fn3">[W]</xref></title><author><name sortKey="Al S, Enriqueta" sort="Al S, Enriqueta" uniqKey="Al S E" first="Enriqueta" last="Al S">Enriqueta Al S</name></author><author><name sortKey="Roca, Maria" sort="Roca, Maria" uniqKey="Roca M" first="María" last="Roca">María Roca</name></author><author><name sortKey="Iglesias, Domingo Jose" sort="Iglesias, Domingo Jose" uniqKey="Iglesias D" first="Domingo José" last="Iglesias">Domingo José Iglesias</name></author><author><name sortKey="Minguez Mosquera, Maria Isabel" sort="Minguez Mosquera, Maria Isabel" uniqKey="Minguez Mosquera M" first="Maria Isabel" last="Mínguez-Mosquera">Maria Isabel Mínguez-Mosquera</name></author><author><name sortKey="Damasceno, Cynthia Maria Borges" sort="Damasceno, Cynthia Maria Borges" uniqKey="Damasceno C" first="Cynthia Maria Borges" last="Damasceno">Cynthia Maria Borges Damasceno</name></author><author><name sortKey="Thannhauser, Theodore William" sort="Thannhauser, Theodore William" uniqKey="Thannhauser T" first="Theodore William" last="Thannhauser">Theodore William Thannhauser</name></author><author><name sortKey="Rose, Jocelyn Kenneth Campbell" sort="Rose, Jocelyn Kenneth Campbell" uniqKey="Rose J" first="Jocelyn Kenneth Campbell" last="Rose">Jocelyn Kenneth Campbell Rose</name></author><author><name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name></author><author><name sortKey="Cerc S, Manuel" sort="Cerc S, Manuel" uniqKey="Cerc S M" first="Manuel" last="Cerc S">Manuel Cerc S</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18467459</idno><idno type="pmc">2442528</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442528</idno><idno type="RBID">PMC:2442528</idno><idno type="doi">10.1104/pp.108.119917</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000819</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the <italic>navel negra</italic> Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis<xref ref-type="fn" rid="fn1">1</xref><xref ref-type="fn" rid="fn3">[W]</xref></title><author><name sortKey="Al S, Enriqueta" sort="Al S, Enriqueta" uniqKey="Al S E" first="Enriqueta" last="Al S">Enriqueta Al S</name></author><author><name sortKey="Roca, Maria" sort="Roca, Maria" uniqKey="Roca M" first="María" last="Roca">María Roca</name></author><author><name sortKey="Iglesias, Domingo Jose" sort="Iglesias, Domingo Jose" uniqKey="Iglesias D" first="Domingo José" last="Iglesias">Domingo José Iglesias</name></author><author><name sortKey="Minguez Mosquera, Maria Isabel" sort="Minguez Mosquera, Maria Isabel" uniqKey="Minguez Mosquera M" first="Maria Isabel" last="Mínguez-Mosquera">Maria Isabel Mínguez-Mosquera</name></author><author><name sortKey="Damasceno, Cynthia Maria Borges" sort="Damasceno, Cynthia Maria Borges" uniqKey="Damasceno C" first="Cynthia Maria Borges" last="Damasceno">Cynthia Maria Borges Damasceno</name></author><author><name sortKey="Thannhauser, Theodore William" sort="Thannhauser, Theodore William" uniqKey="Thannhauser T" first="Theodore William" last="Thannhauser">Theodore William Thannhauser</name></author><author><name sortKey="Rose, Jocelyn Kenneth Campbell" sort="Rose, Jocelyn Kenneth Campbell" uniqKey="Rose J" first="Jocelyn Kenneth Campbell" last="Rose">Jocelyn Kenneth Campbell Rose</name></author><author><name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name></author><author><name sortKey="Cerc S, Manuel" sort="Cerc S, Manuel" uniqKey="Cerc S M" first="Manuel" last="Cerc S">Manuel Cerc S</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A <italic>Citrus sinensis</italic> spontaneous mutant, <italic>navel negra</italic> (<italic>nan</italic>), produces fruit with an abnormal brown-colored flavedo during ripening. Analysis of pigment composition in the wild-type and <italic>nan</italic> flavedo suggested that typical ripening-related chlorophyll (Chl) degradation, but not carotenoid biosynthesis, was impaired in the mutant, identifying <italic>nan</italic> as a type C stay-green mutant. <italic>nan</italic> exhibited normal expression of Chl biosynthetic and catabolic genes and chlorophyllase activity but no accumulation of dephytylated Chl compounds during ripening, suggesting that the mutation is not related to a lesion in any of the principal enzymatic steps in Chl catabolism. Transcript profiling using a citrus microarray indicated that a citrus ortholog of a number of <italic>SGR</italic> (for <italic>STAY-GREEN</italic>) genes was expressed at substantially lower levels in <italic>nan</italic>, both prior to and during ripening. However, the pattern of catabolite accumulation and <italic>SGR</italic> sequence analysis suggested that the <italic>nan</italic> mutation is distinct from those in previously described stay-green mutants and is associated with an upstream regulatory step, rather than directly influencing a specific component of Chl catabolism. Transcriptomic and comparative proteomic profiling further indicated that the <italic>nan</italic> mutation resulted in the suppressed expression of numerous photosynthesis-related genes and in the induction of genes that are associated with oxidative stress. These data, along with metabolite analyses, suggest that <italic>nan</italic> fruit employ a number of molecular mechanisms to compensate for the elevated Chl levels and associated photooxidative stress.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">plantphysiol</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18467459</article-id><article-id pub-id-type="pmc">2442528</article-id><article-id pub-id-type="publisher-id">119917</article-id><article-id pub-id-type="doi">10.1104/pp.108.119917</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Systems Biology, Molecular Biology, and Gene Regulation</subject></subj-group></subj-group></article-categories><title-group><article-title>An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the <italic>navel negra</italic> Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis<xref ref-type="fn" rid="fn1">1</xref><xref ref-type="fn" rid="fn3">[W]</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Alós</surname><given-names>Enriqueta</given-names></name><xref ref-type="fn" rid="fn2">2</xref></contrib><contrib contrib-type="author"><name><surname>Roca</surname><given-names>María</given-names></name></contrib><contrib contrib-type="author"><name><surname>Iglesias</surname><given-names>Domingo José</given-names></name></contrib><contrib contrib-type="author"><name><surname>Mínguez-Mosquera</surname><given-names>Maria Isabel</given-names></name></contrib><contrib contrib-type="author"><name><surname>Damasceno</surname><given-names>Cynthia Maria Borges</given-names></name></contrib><contrib contrib-type="author"><name><surname>Thannhauser</surname><given-names>Theodore William</given-names></name></contrib><contrib contrib-type="author"><name><surname>Rose</surname><given-names>Jocelyn Kenneth Campbell</given-names></name></contrib><contrib contrib-type="author"><name><surname>Talón</surname><given-names>Manuel</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Cercós</surname><given-names>Manuel</given-names></name></contrib></contrib-group><aff id="N0x1cd8f30N0x3ae5d28">Instituto Valenciano de Investigaciones Agrarias, Centro de Genómica, 46113 Moncada, Valencia, Spain (E.A., D.J.I., M.T., M.C.); Chemistry and Biochemistry Pigments Group, Food Biotechnology Department, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, 41012 Sevilla, Spain (M.R., M.I.M.-M.); Department of Plant Biology, Cornell University, Ithaca, New York 14853 (C.M.B.D., J.K.C.R.); and U.S. Department of Agriculture Plant, Soil, and Nutrition Laboratory, Cornell University, Ithaca, New York 14853 (T.W.T.)</aff><author-notes><fn id="cor1"><label>*</label><p>Corresponding author; e-mail <email>talon_man@gva.es</email>.</p></fn><fn id="fn2"><label>2</label><p>Present address: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.</p></fn></author-notes><pub-date pub-type="ppub"><month>7</month><year>2008</year></pub-date><volume>147</volume><issue>3</issue><fpage>1300</fpage><lpage>1315</lpage><history><date date-type="received"><day>27</day><month>3</month><year>2008</year></date><date date-type="accepted"><day>5</day><month>5</month><year>2008</year></date></history><permissions><copyright-statement>Copyright © 2008, American Society of Plant Biologists</copyright-statement></permissions><abstract><p>A <italic>Citrus sinensis</italic> spontaneous mutant, <italic>navel negra</italic> (<italic>nan</italic>), produces fruit with an abnormal brown-colored flavedo during ripening. Analysis of pigment composition in the wild-type and <italic>nan</italic> flavedo suggested that typical ripening-related chlorophyll (Chl) degradation, but not carotenoid biosynthesis, was impaired in the mutant, identifying <italic>nan</italic> as a type C stay-green mutant. <italic>nan</italic> exhibited normal expression of Chl biosynthetic and catabolic genes and chlorophyllase activity but no accumulation of dephytylated Chl compounds during ripening, suggesting that the mutation is not related to a lesion in any of the principal enzymatic steps in Chl catabolism. Transcript profiling using a citrus microarray indicated that a citrus ortholog of a number of <italic>SGR</italic> (for <italic>STAY-GREEN</italic>) genes was expressed at substantially lower levels in <italic>nan</italic>, both prior to and during ripening. However, the pattern of catabolite accumulation and <italic>SGR</italic> sequence analysis suggested that the <italic>nan</italic> mutation is distinct from those in previously described stay-green mutants and is associated with an upstream regulatory step, rather than directly influencing a specific component of Chl catabolism. Transcriptomic and comparative proteomic profiling further indicated that the <italic>nan</italic> mutation resulted in the suppressed expression of numerous photosynthesis-related genes and in the induction of genes that are associated with oxidative stress. These data, along with metabolite analyses, suggest that <italic>nan</italic> fruit employ a number of molecular mechanisms to compensate for the elevated Chl levels and associated photooxidative stress.</p></abstract></article-meta><notes><fn-group><fn id="fn1"><label>1</label><p>This work was supported by the Spanish Ministerio de Ciencia y Tecnología (grant nos. AGL2003–08502–C04–01 and GEN2001–4885–C05–03) and the Instituto Nacional de Investigaciones Agrarias (grant nos. RTA03–106, RTA04–013, and RTA05–247). E.A. was the recipient of an Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) predoctoral fellowship, and D.J.I. and M.C. were the recipients of INIA-Comunidades Autónomas contracts. J.K.C.R. was supported by the National Science Foundation's Plant Genome Program (award no. DBI 0606595).</p></fn><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org">www.plantphysiol.org</ext-link>) is: Manuel Talón (<email>talon_man@gva.es</email>).</p></fn><fn id="fn3"><label>[W]</label><p>The online version of this article contains Web-only data.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org/cgi/doi/10.1104/pp.108.119917">www.plantphysiol.org/cgi/doi/10.1104/pp.108.119917</ext-link></p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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