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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Phenylalanine Ammonia-Lyase Gene Family in Raspberry.
Structure, Expression, and Evolution<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Kumar, Amrita" sort="Kumar, Amrita" uniqKey="Kumar A" first="Amrita" last="Kumar">Amrita Kumar</name></author><author><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E." last="Ellis">Brian E. Ellis</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11553751</idno><idno type="pmc">117979</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117979</idno><idno type="RBID">PMC:117979</idno><date when="2001">2001</date><idno type="wicri:Area/Pmc/Corpus">000536</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Phenylalanine Ammonia-Lyase Gene Family in Raspberry.
Structure, Expression, and Evolution<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Kumar, Amrita" sort="Kumar, Amrita" uniqKey="Kumar A" first="Amrita" last="Kumar">Amrita Kumar</name></author><author><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E." last="Ellis">Brian E. Ellis</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In raspberry (<italic>Rubus idaeus</italic>), development of fruit
color and flavor are critically dependent on products of the
phenylpropanoid pathway. To determine how these metabolic functions are
integrated with the fruit ripening program, we are examining the
properties and expression of key genes in the pathway. Here, we report
that <sc>l</sc>- phenylalanine ammonia-lyase (PAL) is encoded in
raspberry by a family of two genes (<italic>RiPAL1</italic> and
<italic>RiPAL2</italic>). <italic>RiPAL1</italic> shares 88% amino acid
sequence similarity to <italic>RiPAL2</italic>, but phylogenetic analysis
places <italic>RiPAL1</italic> and <italic>RiPAL2</italic> in different
clusters within the plant <italic>PAL</italic> gene family. The spatial
and temporal expression patterns of the two genes were investigated in
various vegetative and floral tissues using the reverse transcriptase
competitor polymerase chain reaction assay. Although expression of both
genes was detected in all tissues examined, <italic>RiPAL1</italic> was
associated with early fruit ripening events, whereas expression of
<italic>RiPAL2</italic> correlated more with later stages of flower and
fruit development. Determination of the absolute levels of the two
transcripts in various tissues showed that <italic>RiPAL1</italic>transcripts were 3- to 10-fold more abundant than those of
<italic>RiPAL2</italic> in leaves, shoots, roots, young fruits, and ripe
fruits. The two <italic>RiPAL</italic> genes therefore appear to be
controlled by different regulatory mechanisms.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">PLANT PHYSIOL</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Physiologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11553751</article-id><article-id pub-id-type="pmc">117979</article-id><article-id pub-id-type="publisher-id">6815</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Genetics, Genomics, and Molecular Evolution</subject></subj-group></subj-group></article-categories><title-group><article-title>The Phenylalanine Ammonia-Lyase Gene Family in Raspberry.
Structure, Expression, and Evolution<xref ref-type="fn" rid="FN1">1</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kumar</surname><given-names>Amrita</given-names></name><xref ref-type="author-notes" rid="FN2">2</xref></contrib><contrib contrib-type="author"><name><surname>Ellis</surname><given-names>Brian E.</given-names></name><xref ref-type="author-notes" rid="FN150">*</xref></contrib></contrib-group><aff id="N0xac6cf80N0xad60434">The Biotechnology Laboratory and Faculty of Agricultural Sciences, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4</aff><author-notes><fn id="FN2"><label>2</label><p>Present address: Department of Microbiology and
Immunology, Emory University School of Medicine, 1510 Clifton Road,
Atlanta, GA 30322.</p></fn><fn id="FN150"><label>*</label><p>Corresponding author; e-mail <email>bee@interchg.ubc.ca</email>; fax
604–822–8640.</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2001</year></pub-date><volume>127</volume><issue>1</issue><fpage>230</fpage><lpage>239</lpage><history><date date-type="received"><day>23</day><month>4</month><year>2001</year></date><date date-type="accepted"><day>9</day><month>6</month><year>2001</year></date></history><copyright-statement>Copyright © 2001, American Society of Plant Physiologists</copyright-statement><copyright-year>2001</copyright-year><abstract><p>In raspberry (<italic>Rubus idaeus</italic>), development of fruit
color and flavor are critically dependent on products of the
phenylpropanoid pathway. To determine how these metabolic functions are
integrated with the fruit ripening program, we are examining the
properties and expression of key genes in the pathway. Here, we report
that <sc>l</sc>- phenylalanine ammonia-lyase (PAL) is encoded in
raspberry by a family of two genes (<italic>RiPAL1</italic> and
<italic>RiPAL2</italic>). <italic>RiPAL1</italic> shares 88% amino acid
sequence similarity to <italic>RiPAL2</italic>, but phylogenetic analysis
places <italic>RiPAL1</italic> and <italic>RiPAL2</italic> in different
clusters within the plant <italic>PAL</italic> gene family. The spatial
and temporal expression patterns of the two genes were investigated in
various vegetative and floral tissues using the reverse transcriptase
competitor polymerase chain reaction assay. Although expression of both
genes was detected in all tissues examined, <italic>RiPAL1</italic> was
associated with early fruit ripening events, whereas expression of
<italic>RiPAL2</italic> correlated more with later stages of flower and
fruit development. Determination of the absolute levels of the two
transcripts in various tissues showed that <italic>RiPAL1</italic>transcripts were 3- to 10-fold more abundant than those of
<italic>RiPAL2</italic> in leaves, shoots, roots, young fruits, and ripe
fruits. The two <italic>RiPAL</italic> genes therefore appear to be
controlled by different regulatory mechanisms.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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