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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Novel Cellulosomal Scaffoldin from <italic>Acetivibrio cellulolyticus</italic> That Contains a Family 9 Glycosyl Hydrolase</title><author><name sortKey="Ding, Shi You" sort="Ding, Shi You" uniqKey="Ding S" first="Shi-You" last="Ding">Shi-You Ding</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Bayer, Edward A" sort="Bayer, Edward A" uniqKey="Bayer E" first="Edward A." last="Bayer">Edward A. Bayer</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Steiner, David" sort="Steiner, David" uniqKey="Steiner D" first="David" last="Steiner">David Steiner</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Shoham, Yuval" sort="Shoham, Yuval" uniqKey="Shoham Y" first="Yuval" last="Shoham">Yuval Shoham</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Lamed, Raphael" sort="Lamed, Raphael" uniqKey="Lamed R" first="Raphael" last="Lamed">Raphael Lamed</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">10542174</idno><idno type="pmc">94137</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94137</idno><idno type="RBID">PMC:94137</idno><date when="1999">1999</date><idno type="wicri:Area/Pmc/Corpus">000498</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Novel Cellulosomal Scaffoldin from <italic>Acetivibrio cellulolyticus</italic> That Contains a Family 9 Glycosyl Hydrolase</title><author><name sortKey="Ding, Shi You" sort="Ding, Shi You" uniqKey="Ding S" first="Shi-You" last="Ding">Shi-You Ding</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Bayer, Edward A" sort="Bayer, Edward A" uniqKey="Bayer E" first="Edward A." last="Bayer">Edward A. Bayer</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Steiner, David" sort="Steiner, David" uniqKey="Steiner D" first="David" last="Steiner">David Steiner</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Shoham, Yuval" sort="Shoham, Yuval" uniqKey="Shoham Y" first="Yuval" last="Shoham">Yuval Shoham</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author><author><name sortKey="Lamed, Raphael" sort="Lamed, Raphael" uniqKey="Lamed R" first="Raphael" last="Lamed">Raphael Lamed</name><affiliation><nlm:aff id="N0x972d0f8.0x9ca4630"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Bacteriology</title><idno type="ISSN">0021-9193</idno><idno type="eISSN">1098-5530</idno><imprint><date when="1999">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A novel cellulosomal scaffoldin gene, termed <italic>cipV</italic>, was identified and sequenced from the mesophilic cellulolytic anaerobe <italic>Acetivibrio cellulolyticus</italic>. Initial identification of the protein was based on a combination of properties, including its high molecular weight, cellulose-binding activity, glycoprotein nature, and immuno-cross-reactivity with the cellulosomal scaffoldin of <italic>Clostridium thermocellum</italic>. The <italic>cipV</italic> gene is 5,748 bp in length and encodes a 1,915-residue polypeptide with a calculated molecular weight of 199,496. CipV contains an N-terminal signal peptide, seven type I cohesin domains, an internal family III cellulose-binding domain (CBD), and an X2 module of unknown function in tandem with a type II dockerin domain at the C terminus. Surprisingly, CipV also possesses at its N terminus a catalytic module that belongs to the family 9 glycosyl hydrolases. Sequence analysis indicated the following. (i) The repeating cohesin domains are very similar to each other, ranging between 70 and 90% identity, and they also have about 30 to 40% homology with each of the other known type I scaffoldin cohesins. (ii) The internal CBD belongs to family III but differs from other known scaffoldin CBDs by the omission of a 9-residue stretch that constitutes a characteristic loop previously associated with the scaffoldins. (iii) The C-terminal type II dockerin domain is only the second such domain to have been discovered; its predicted “recognition codes” differ from those proposed for the other known dockerins. The putative calcium-binding loop includes an unusual insert, lacking in all the known type I and type II dockerins. (iv) The X2 module has about 60% sequence homology with that of <italic>C. thermocellum</italic> and appears at the same position in the scaffoldin. (v) Unlike the other known family 9 catalytic modules of bacterial origin, the CipV catalytic module is not accompanied by a flanking helper module, e.g., an adjacent family IIIc CBD or an immunoglobulin-like domain. Comparative sequence analysis of the CipV functional modules with those of the previously sequenced scaffoldins provides new insight into the structural arrangement and phylogeny of this intriguing family of microbial proteins. The modular organization of CipV is reminiscent of that of the CipA scaffoldin from <italic>C. thermocellum</italic> as opposed to the known scaffoldins from the mesophilic clostridia. The phylogenetic relationship of the different functional modules appears to indicate that the evolution of the scaffoldins reflects a collection of independent events and mechanisms whereby individual modules and other constituents are incorporated into the scaffoldin gene from different microbial sources.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Bacteriol</journal-id><journal-id journal-id-type="publisher-id">J BACTERIOL</journal-id><journal-title>Journal of Bacteriology</journal-title><issn pub-type="ppub">0021-9193</issn><issn pub-type="epub">1098-5530</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">10542174</article-id><article-id pub-id-type="pmc">94137</article-id><article-id pub-id-type="publisher-id">0777</article-id><article-categories><subj-group subj-group-type="heading"><subject>Enzymes and Proteins</subject></subj-group></article-categories><title-group><article-title>A Novel Cellulosomal Scaffoldin from <italic>Acetivibrio cellulolyticus</italic> That Contains a Family 9 Glycosyl Hydrolase</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Ding</surname><given-names>Shi-You</given-names></name><xref ref-type="aff" rid="N0x972d0f8.0x9ca4630">1</xref><xref ref-type="aff" rid="N0x972d0f8.0x9ca4630">2</xref></contrib><contrib contrib-type="author"><name><surname>Bayer</surname><given-names>Edward A.</given-names></name><xref ref-type="aff" rid="N0x972d0f8.0x9ca4630">1</xref><xref ref-type="author-notes" rid="FN150">*</xref></contrib><contrib contrib-type="author"><name><surname>Steiner</surname><given-names>David</given-names></name><xref ref-type="aff" rid="N0x972d0f8.0x9ca4630">2</xref></contrib><contrib contrib-type="author"><name><surname>Shoham</surname><given-names>Yuval</given-names></name><xref ref-type="aff" rid="N0x972d0f8.0x9ca4630">3</xref></contrib><contrib contrib-type="author"><name><surname>Lamed</surname><given-names>Raphael</given-names></name><xref ref-type="aff" rid="N0x972d0f8.0x9ca4630">2</xref></contrib></contrib-group><aff id="N0x972d0f8.0x9ca4630"> Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot,<sup>1</sup> Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat Aviv,<sup>2</sup> and Department of Food Engineering and Biotechnology, Technion—Israel Institute of Technology, Haifa,<sup>3</sup> Israel</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author. Mailing address: Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel. Phone: (972)-8-934-2373. Fax: (972)-8-946-8256. E-mail: <email>bfbayer@weizmann.weizmann.ac.il</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>11</month><year>1999</year></pub-date><volume>181</volume><issue>21</issue><fpage>6720</fpage><lpage>6729</lpage><history><date date-type="received"><day>10</day><month>6</month><year>1999</year></date><date date-type="accepted"><day>24</day><month>8</month><year>1999</year></date></history><copyright-statement>Copyright © 1999, American Society for Microbiology</copyright-statement><copyright-year>1999</copyright-year><abstract><p>A novel cellulosomal scaffoldin gene, termed <italic>cipV</italic>, was identified and sequenced from the mesophilic cellulolytic anaerobe <italic>Acetivibrio cellulolyticus</italic>. Initial identification of the protein was based on a combination of properties, including its high molecular weight, cellulose-binding activity, glycoprotein nature, and immuno-cross-reactivity with the cellulosomal scaffoldin of <italic>Clostridium thermocellum</italic>. The <italic>cipV</italic> gene is 5,748 bp in length and encodes a 1,915-residue polypeptide with a calculated molecular weight of 199,496. CipV contains an N-terminal signal peptide, seven type I cohesin domains, an internal family III cellulose-binding domain (CBD), and an X2 module of unknown function in tandem with a type II dockerin domain at the C terminus. Surprisingly, CipV also possesses at its N terminus a catalytic module that belongs to the family 9 glycosyl hydrolases. Sequence analysis indicated the following. (i) The repeating cohesin domains are very similar to each other, ranging between 70 and 90% identity, and they also have about 30 to 40% homology with each of the other known type I scaffoldin cohesins. (ii) The internal CBD belongs to family III but differs from other known scaffoldin CBDs by the omission of a 9-residue stretch that constitutes a characteristic loop previously associated with the scaffoldins. (iii) The C-terminal type II dockerin domain is only the second such domain to have been discovered; its predicted “recognition codes” differ from those proposed for the other known dockerins. The putative calcium-binding loop includes an unusual insert, lacking in all the known type I and type II dockerins. (iv) The X2 module has about 60% sequence homology with that of <italic>C. thermocellum</italic> and appears at the same position in the scaffoldin. (v) Unlike the other known family 9 catalytic modules of bacterial origin, the CipV catalytic module is not accompanied by a flanking helper module, e.g., an adjacent family IIIc CBD or an immunoglobulin-like domain. Comparative sequence analysis of the CipV functional modules with those of the previously sequenced scaffoldins provides new insight into the structural arrangement and phylogeny of this intriguing family of microbial proteins. The modular organization of CipV is reminiscent of that of the CipA scaffoldin from <italic>C. thermocellum</italic> as opposed to the known scaffoldins from the mesophilic clostridia. The phylogenetic relationship of the different functional modules appears to indicate that the evolution of the scaffoldins reflects a collection of independent events and mechanisms whereby individual modules and other constituents are incorporated into the scaffoldin gene from different microbial sources.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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