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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structures, Functions, and Interactions of ClpT1 and ClpT2 in the Clp Protease System of Arabidopsis Chloroplasts</title><author><name sortKey="Kim, Jitae" sort="Kim, Jitae" uniqKey="Kim J" first="Jitae" last="Kim">Jitae Kim</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Kimber, Matthew S" sort="Kimber, Matthew S" uniqKey="Kimber M" first="Matthew S." last="Kimber">Matthew S. Kimber</name><affiliation><nlm:aff id="aff2">Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada</nlm:aff></affiliation></author><author><name sortKey="Nishimura, Kenji" sort="Nishimura, Kenji" uniqKey="Nishimura K" first="Kenji" last="Nishimura">Kenji Nishimura</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Friso, Giulia" sort="Friso, Giulia" uniqKey="Friso G" first="Giulia" last="Friso">Giulia Friso</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Schultz, Lance" sort="Schultz, Lance" uniqKey="Schultz L" first="Lance" last="Schultz">Lance Schultz</name><affiliation><nlm:aff id="aff2">Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada</nlm:aff></affiliation></author><author><name sortKey="Ponnala, Lalit" sort="Ponnala, Lalit" uniqKey="Ponnala L" first="Lalit" last="Ponnala">Lalit Ponnala</name><affiliation><nlm:aff id="aff3">Computational Biology Service Unit, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Van Wijk, Klaas J" sort="Van Wijk, Klaas J" uniqKey="Van Wijk K" first="Klaas J." last="Van Wijk">Klaas J. Van Wijk</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25921872</idno><idno type="pmc">4456643</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456643</idno><idno type="RBID">PMC:4456643</idno><idno type="doi">10.1105/tpc.15.00106</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000017</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structures, Functions, and Interactions of ClpT1 and ClpT2 in the Clp Protease System of Arabidopsis Chloroplasts</title><author><name sortKey="Kim, Jitae" sort="Kim, Jitae" uniqKey="Kim J" first="Jitae" last="Kim">Jitae Kim</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Kimber, Matthew S" sort="Kimber, Matthew S" uniqKey="Kimber M" first="Matthew S." last="Kimber">Matthew S. Kimber</name><affiliation><nlm:aff id="aff2">Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada</nlm:aff></affiliation></author><author><name sortKey="Nishimura, Kenji" sort="Nishimura, Kenji" uniqKey="Nishimura K" first="Kenji" last="Nishimura">Kenji Nishimura</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Friso, Giulia" sort="Friso, Giulia" uniqKey="Friso G" first="Giulia" last="Friso">Giulia Friso</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Schultz, Lance" sort="Schultz, Lance" uniqKey="Schultz L" first="Lance" last="Schultz">Lance Schultz</name><affiliation><nlm:aff id="aff2">Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada</nlm:aff></affiliation></author><author><name sortKey="Ponnala, Lalit" sort="Ponnala, Lalit" uniqKey="Ponnala L" first="Lalit" last="Ponnala">Lalit Ponnala</name><affiliation><nlm:aff id="aff3">Computational Biology Service Unit, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author><author><name sortKey="Van Wijk, Klaas J" sort="Van Wijk, Klaas J" uniqKey="Van Wijk K" first="Klaas J." last="Van Wijk">Klaas J. Van Wijk</name><affiliation><nlm:aff id="aff1">Department of Plant Biology, Cornell University, Ithaca, New York 14853</nlm:aff></affiliation></author></analytic><series><title level="j">The Plant Cell</title><idno type="ISSN">1040-4651</idno><idno type="eISSN">1532-298X</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Plant-specific ClpT proteins support stabilization and capacity of the tetradecameric ClpPR protease function in chloroplasts through interactions with the ClpP ring.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id><journal-id journal-id-type="iso-abbrev">Plant Cell</journal-id><journal-id journal-id-type="hwp">plantcell</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>The Plant Cell</journal-title></journal-title-group><issn pub-type="ppub">1040-4651</issn><issn pub-type="epub">1532-298X</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25921872</article-id><article-id pub-id-type="pmc">4456643</article-id><article-id pub-id-type="publisher-id">TPC201500106RAR1</article-id><article-id pub-id-type="doi">10.1105/tpc.15.00106</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject></subj-group></article-categories><title-group><article-title>Structures, Functions, and Interactions of ClpT1 and ClpT2 in the Clp Protease System of Arabidopsis Chloroplasts</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Jitae</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kimber</surname><given-names>Matthew S.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nishimura</surname><given-names>Kenji</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Friso</surname><given-names>Giulia</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Schultz</surname><given-names>Lance</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ponnala</surname><given-names>Lalit</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0001-9536-0487</contrib-id><name><surname>van Wijk</surname><given-names>Klaas J.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1"><label>a</label>Department of Plant Biology, Cornell University, Ithaca, New York 14853</aff><aff id="aff2"><label>b</label>Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada</aff><aff id="aff3"><label>c</label>Computational Biology Service Unit, Cornell University, Ithaca, New York 14853</aff></contrib-group><author-notes><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantcell.org/cgi/doi/10.1105/tpc.15.00106">www.plantcell.org/cgi/doi/10.1105/tpc.15.00106</ext-link></p></fn><corresp id="cor1"><label>1</label>Address correspondence to <email>kv35@cornell.edu</email>.</corresp><fn id="afn1"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantcell.org">www.plantcell.org</ext-link>) is: Klaas J. van Wijk (<email>kv35@cornell.edu</email>).</p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>5</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>28</day><month>4</month><year>2015</year></pub-date><volume>27</volume><issue>5</issue><fpage>1477</fpage><lpage>1496</lpage><history><date date-type="received"><day>04</day><month>2</month><year>2015</year></date><date date-type="rev-recd"><day>19</day><month>3</month><year>2015</year></date><date date-type="accepted"><day>08</day><month>4</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 American Society of Plant Biologists. All rights reserved.</copyright-statement><copyright-year>2015</copyright-year></permissions><self-uri xlink:role="icon" xlink:href="PC_TPC201500106RAR1_ic1.gif"></self-uri><abstract abstract-type="precis"><p>Plant-specific ClpT proteins support stabilization and capacity of the tetradecameric ClpPR protease function in chloroplasts through interactions with the ClpP ring.</p></abstract><abstract><p>Plastid ClpT1 and ClpT2 are plant-specific proteins that associate with the ClpPR protease. However, their physiological significance and structures are not understood. <italic>Arabidopsis thaliana</italic> loss-of-function single <italic>clpt1</italic> and <italic>clpt2</italic> mutants showed no visible phenotypes, whereas <italic>clpt1 clpt2</italic> double mutants showed delayed development, reduced plant growth, and virescent, serrated leaves but were viable and produced seed. The <italic>clpt1</italic> and <italic>clpt1 clpt2</italic> mutants showed partial destabilization of the ClpPR complex, whereas <italic>clpt2</italic> null mutants showed only marginal destabilization. Comparative proteomics of <italic>clpt1 clpt2</italic> plants showed a proteostasis phenotype similar to viable mutants in ClpPR core subunits, indicating reduced Clp protease capacity. In vivo and in vitro assays showed that ClpT1 and ClpT2 can independently interact with the single ClpP ring and ClpPR core, but not with the single ClpR ring. We determined ClpT1 and ClpT2 structures (2.4- and 2.0-Å resolution) and detailed the similarities to the N-domains of bacterial ClpA/C chaperones. The ClpT structures suggested a conserved MYFF motif for interaction with the ClpPR core near the interface between the P- and R-rings. In vivo complementation showed that ClpT function and ClpPR core stabilization require the MYFF motif. Several models are presented that may explain how ClpT1,2 contribute to ClpPR protease activity.</p></abstract><counts><page-count count="20"></page-count></counts><custom-meta-group><custom-meta><meta-name> DJS Export </meta-name><meta-value>v1</meta-value></custom-meta></custom-meta-group></article-meta><notes><glossary><title>Glossary</title><def-list><def-item><term id="term1">MS</term><def id="def1"><p>mass spectrometry</p></def></def-item><def-item><term id="term2">MS/MS</term><def id="def2"><p>tandem mass spectrometry</p></def></def-item><def-item><term id="term3">1D-BN-PAGE</term><def id="def3"><p>one-dimensional native gel electrophoresis</p></def></def-item><def-item><term id="term4">r.m.s.d.</term><def id="def4"><p>root mean square deviation</p></def></def-item><def-item><term id="term5">BN-PAGE</term><def id="def5"><p>blue native PAGE</p></def></def-item><def-item><term id="term6">CN-PAGE</term><def id="def6"><p>colorless native PAGE</p></def></def-item></def-list></glossary></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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