Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba[W][OPEN]
Identifieur interne : 000016 ( Pmc/Corpus ); précédent : 000015; suivant : 000017Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba[W][OPEN]
Auteurs : Fangqin Zeng ; Bifang ChengSource :
- The Plant Cell [ 1040-4651 ] ; 2014.
Abstract
Using the
Url:
DOI: 10.1105/tpc.114.126631
PubMed: 24934174
PubMed Central: 4114957
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PMC:4114957Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of <italic>FAE1</italic> in <italic>Sinapis alba</italic><xref ref-type="fn" rid="fn1"><sup>[W]</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Zeng, Fangqin" sort="Zeng, Fangqin" uniqKey="Zeng F" first="Fangqin" last="Zeng">Fangqin Zeng</name></author><author><name sortKey="Cheng, Bifang" sort="Cheng, Bifang" uniqKey="Cheng B" first="Bifang" last="Cheng">Bifang Cheng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24934174</idno><idno type="pmc">4114957</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4114957</idno><idno type="RBID">PMC:4114957</idno><idno type="doi">10.1105/tpc.114.126631</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000016</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of <italic>FAE1</italic> in <italic>Sinapis alba</italic><xref ref-type="fn" rid="fn1"><sup>[W]</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></title><author><name sortKey="Zeng, Fangqin" sort="Zeng, Fangqin" uniqKey="Zeng F" first="Fangqin" last="Zeng">Fangqin Zeng</name></author><author><name sortKey="Cheng, Bifang" sort="Cheng, Bifang" uniqKey="Cheng B" first="Bifang" last="Cheng">Bifang Cheng</name></author></analytic><series><title level="j">The Plant Cell</title><idno type="ISSN">1040-4651</idno><idno type="eISSN">1532-298X</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Using the <italic>FATTY ACID ELONGATION1</italic> (<italic>FAE1</italic>) gene as a case study, this work reveals that transposable element insertion and epigenetic modification generate multiple heritable alleles varying in expression level and modulating trait phenotype from qualitative to quantitative variation.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id><journal-id journal-id-type="iso-abbrev">Plant Cell</journal-id><journal-id journal-id-type="hwp">plantcell</journal-id><journal-id journal-id-type="publisher-id">aspb</journal-id><journal-title-group><journal-title>The Plant Cell</journal-title></journal-title-group><issn pub-type="ppub">1040-4651</issn><issn pub-type="epub">1532-298X</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24934174</article-id><article-id pub-id-type="pmc">4114957</article-id><article-id pub-id-type="publisher-id">126631</article-id><article-id pub-id-type="doi">10.1105/tpc.114.126631</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject></subj-group></article-categories><title-group><article-title>Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of <italic>FAE1</italic> in <italic>Sinapis alba</italic><xref ref-type="fn" rid="fn1"><sup>[W]</sup></xref><xref ref-type="fn" rid="fn2"><sup>[OPEN]</sup></xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zeng</surname><given-names>Fangqin</given-names></name></contrib><contrib contrib-type="author"><name><surname>Cheng</surname><given-names>Bifang</given-names></name><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1">Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon S7N 0X2, Canada</aff></contrib-group><author-notes><fn><p><ext-link ext-link-type="uri" xlink:href="http://www.plantcell.org/cgi/doi/10.1105/tpc.114.126631">www.plantcell.org/cgi/doi/10.1105/tpc.114.126631</ext-link></p></fn><corresp id="cor1"><label>1</label>Address correspondence to <email>bifang.cheng@agr.gc.ca</email>.</corresp><fn id="afn1"><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="http://www.plantcell.org">www.plantcell.org</ext-link>) is: Bifang Cheng (<email>bifang.cheng@agr.gc.ca</email>).</p></fn></author-notes><pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>6</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>16</day><month>6</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>16</day><month>6</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>26</volume><issue>6</issue><fpage>2648</fpage><lpage>2659</lpage><history><date date-type="received"><day>16</day><month>4</month><year>2014</year></date><date date-type="rev-recd"><day>19</day><month>5</month><year>2014</year></date><date date-type="accepted"><day>27</day><month>5</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada.</copyright-statement><copyright-year>2014</copyright-year></permissions><self-uri xlink:role="icon" xlink:type="simple" xlink:href="PC_126631_ic1.gif"></self-uri><abstract abstract-type="precis"><p>Using the <italic>FATTY ACID ELONGATION1</italic> (<italic>FAE1</italic>) gene as a case study, this work reveals that transposable element insertion and epigenetic modification generate multiple heritable alleles varying in expression level and modulating trait phenotype from qualitative to quantitative variation.</p></abstract><abstract><p>Naturally occurring heritable variation provides a fundamental resource to reveal the genetic and molecular bases of traits in forward genetic studies. Here, we report the molecular basis of the differences in the four alleles <italic>E<sup>1</sup></italic>, <italic>E<sup>2</sup></italic>, <italic>E<sup>3</sup></italic>, and <italic>e</italic> of the <italic>FATTY ACID ELONGATION1</italic> (<italic>FAE1</italic>) gene controlling high, medium, low, and zero erucic content in yellow mustard (<italic>Sinapis alba</italic>). <italic>E<sup>1</sup></italic> represents a fully functional allele with a coding DNA sequence (<xref ref-type="def" rid="def3">CDS</xref>) of 1521 bp and a promoter adjacent to the <xref ref-type="def" rid="def3">CDS</xref>. The null allele <italic>e</italic> resulted from an insertional disruption in the <xref ref-type="def" rid="def3">CDS</xref> by Sal-<italic>PIF</italic>, a 3100-bp <italic>PIF/Harbinger</italic>-like DNA transposon, whereas <italic>E<sup>2</sup></italic> and <italic>E<sup>3</sup></italic> originated from the insertion of Sal-<italic>T1</italic>, a 4863-bp Copia-like retrotransposon, in the 5′ untranslated region. <italic>E<sup>3</sup></italic> was identical to <italic>E<sup>2</sup></italic> but showed cytosine methylation in the promoter region and was thus an epiallele having a further reduction in expression. The coding regions of <italic>E<sup>2</sup></italic> and <italic>E<sup>3</sup></italic> also contained five single-nucleotide polymorphisms (<xref ref-type="def" rid="def1">SNPs</xref>) not present in <italic>E<sup>1</sup></italic>, but expression studies in <italic>Saccharomyces cerevisiae</italic> indicated that these <xref ref-type="def" rid="def1">SNPs</xref> did not affect enzyme functionality. These results demonstrate a comprehensive molecular framework for the interplay of transposon insertion, <xref ref-type="def" rid="def1">SNP</xref>/indel mutation, and epigenetic modification influencing the broad range of natural genetic variation in plants.</p></abstract><counts><page-count count="12"></page-count></counts></article-meta><notes><glossary><title>Glossary</title><def-list><def-item><term id="term1">SNP</term><def id="def1"><p>single-nucleotide polymorphism</p></def></def-item><def-item><term id="term2">TE</term><def id="def2"><p>transposable element</p></def></def-item><def-item><term id="term3">CDS</term><def id="def3"><p>coding DNA sequence</p></def></def-item><def-item><term id="term4">qPCR</term><def id="def4"><p>quantitative PCR</p></def></def-item><def-item><term id="term5">UTR</term><def id="def5"><p>untranslated region</p></def></def-item><def-item><term id="term6">PBS</term><def id="def6"><p>primer binding site</p></def></def-item><def-item><term id="term7">PPT</term><def id="def7"><p>polypurine tract</p></def></def-item><def-item><term id="term8">LTR</term><def id="def8"><p>long terminal repeat</p></def></def-item><def-item><term id="term9">5-azaC</term><def id="def9"><p>5-azacytidine</p></def></def-item></def-list></glossary></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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