Single-cell-derived sibling lines are established as an experimental system to assess chromosome number variations in embryogenic callus cultures of sweet orange
Identifieur interne : 000728 ( PascalFrancis/Corpus ); précédent : 000727; suivant : 000729Single-cell-derived sibling lines are established as an experimental system to assess chromosome number variations in embryogenic callus cultures of sweet orange
Auteurs : Yu-Jin Hao ; Xiu-Xin DengSource :
- Plant cell, tissue and organ culture [ 0167-6857 ] ; 2003.
Abstract
Single-cell derived sibling lines of 'Anliucheng' sweet orange (Citrus sinensis (L.) Osbeck) were established with low-density protoplast culture. Cell densities of 1 to 5×104 cell.ml-1 were appropriate for this purpose, while a super-low cell density of 5X103 cell.ml-1 made most protoplasts destined for programmed cell death (PCD) characterized by multi-nucleation and the formation of apoptotic bodies. These single-cell derived sibling lines were then used as an experimental system for genetic assessment. Cytological examination revealed that chromosome number variation arose spontaneously in the colony per se but not from pre-existing variation. Chromosome number variations continued after one year of subculture at 1-month intervals. However, the ploidy level of these cell lines remained relatively stable during the culture period. In addition, these chromosome number variations were selected against during plant regeneration process.
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| NO : | PASCAL 03-0478429 INIST |
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| ET : | Single-cell-derived sibling lines are established as an experimental system to assess chromosome number variations in embryogenic callus cultures of sweet orange |
| AU : | HAO (Yu-Jin); DENG (Xiu-Xin) |
| AF : | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University/Wuhan 430070/Chine (1 aut., 2 aut.) |
| DT : | Publication en série; Niveau analytique |
| SO : | Plant cell, tissue and organ culture; ISSN 0167-6857; Coden PTCEDJ; Pays-Bas; Da. 2003; Vol. 73; No. 3; Pp. 275-280; Bibl. 15 ref. |
| LA : | Anglais |
| EA : | Single-cell derived sibling lines of 'Anliucheng' sweet orange (Citrus sinensis (L.) Osbeck) were established with low-density protoplast culture. Cell densities of 1 to 5×104 cell.ml-1 were appropriate for this purpose, while a super-low cell density of 5X103 cell.ml-1 made most protoplasts destined for programmed cell death (PCD) characterized by multi-nucleation and the formation of apoptotic bodies. These single-cell derived sibling lines were then used as an experimental system for genetic assessment. Cytological examination revealed that chromosome number variation arose spontaneously in the colony per se but not from pre-existing variation. Chromosome number variations continued after one year of subculture at 1-month intervals. However, the ploidy level of these cell lines remained relatively stable during the culture period. In addition, these chromosome number variations were selected against during plant regeneration process. |
| CC : | 002A |
| LO : | INIST-19374.354000111018140080 |
| ID : | 03-0478429 |
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Pascal:03-0478429Le document en format XML
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Osbeck) were established with low-density protoplast culture. Cell densities of 1 to 5×10<sup>4</sup> cell.ml<sup>-1</sup> were appropriate for this purpose, while a super-low cell density of 5X10<sup>3</sup> cell.ml<sup>-1</sup> made most protoplasts destined for programmed cell death (PCD) characterized by multi-nucleation and the formation of apoptotic bodies. These single-cell derived sibling lines were then used as an experimental system for genetic assessment. Cytological examination revealed that chromosome number variation arose spontaneously in the colony per se but not from pre-existing variation. Chromosome number variations continued after one year of subculture at 1-month intervals. However, the ploidy level of these cell lines remained relatively stable during the culture period. 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All rights reserved.</s1></fA44><fA45><s0>15 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>03-0478429</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Plant cell, tissue and organ culture</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>Single-cell derived sibling lines of 'Anliucheng' sweet orange (Citrus sinensis (L.) Osbeck) were established with low-density protoplast culture. Cell densities of 1 to 5×10<sup>4</sup> cell.ml<sup>-1</sup> were appropriate for this purpose, while a super-low cell density of 5X10<sup>3</sup> cell.ml<sup>-1</sup> made most protoplasts destined for programmed cell death (PCD) characterized by multi-nucleation and the formation of apoptotic bodies. These single-cell derived sibling lines were then used as an experimental system for genetic assessment. Cytological examination revealed that chromosome number variation arose spontaneously in the colony per se but not from pre-existing variation. 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In addition, these chromosome number variations were selected against during plant regeneration process.</s0></fC01><fC02 i1="01" i2="X"><s0>002A</s0></fC02><fN21><s1>321</s1></fN21><fN82><s1>DST</s1></fN82></pA></standard><server><NO>PASCAL 03-0478429 INIST</NO><ET>Single-cell-derived sibling lines are established as an experimental system to assess chromosome number variations in embryogenic callus cultures of sweet orange</ET><AU>HAO (Yu-Jin); DENG (Xiu-Xin)</AU><AF>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University/Wuhan 430070/Chine (1 aut., 2 aut.)</AF><DT>Publication en série; Niveau analytique</DT><SO>Plant cell, tissue and organ culture; ISSN 0167-6857; Coden PTCEDJ; Pays-Bas; Da. 2003; Vol. 73; No. 3; Pp. 275-280; Bibl. 15 ref.</SO><LA>Anglais</LA><EA>Single-cell derived sibling lines of 'Anliucheng' sweet orange (Citrus sinensis (L.) Osbeck) were established with low-density protoplast culture. Cell densities of 1 to 5×10<sup>4</sup> cell.ml<sup>-1</sup> were appropriate for this purpose, while a super-low cell density of 5X10<sup>3</sup> cell.ml<sup>-1</sup> made most protoplasts destined for programmed cell death (PCD) characterized by multi-nucleation and the formation of apoptotic bodies. These single-cell derived sibling lines were then used as an experimental system for genetic assessment. Cytological examination revealed that chromosome number variation arose spontaneously in the colony per se but not from pre-existing variation. Chromosome number variations continued after one year of subculture at 1-month intervals. However, the ploidy level of these cell lines remained relatively stable during the culture period. In addition, these chromosome number variations were selected against during plant regeneration process.</EA><CC>002A</CC><LO>INIST-19374.354000111018140080</LO><ID>03-0478429</ID></server></inist></record>Pour manipuler ce document sous Unix (Dilib)
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