OrangerV1, PascalFrancis, Corpus, bibRecord, 000727

Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Identifieur interne : 000727 ( PascalFrancis/Corpus ); précédent : 000726; suivant : 000728

Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Auteurs : A. K. Kobayashi ; J. C. Bespalhok ; L. F. P. Pereira ; L. G. E. Vieira

Source :

Plant cell, tissue and organ culture [ 0167-6857 ] ; 2003.

RBID : Pascal:03-0479314

Abstract

An efficient system for in vitro plant regeneration from thin transversal stem sections explants (1-2 mm) using mature tissues of sweet orange cv. Pera was developed. Explants were cultured in different media to evaluate the frequency of regeneration and size of buds. A high percentage of explants (54% with 3.1 buds/explant) producing large buds (1-4 mm) was observed when the explants were cultivated for 2 weeks on Murashige and Skoog medium and then transferred to Woody Plant medium (WPM). Both media were supplemented with 1.8 μM 6-benzylaminopurine and 0.7 μM gibberellic acid. Adventitious buds were regenerated into whole plants by in vitro shoot-tip grafting. Regenerated plants started to flower after 12 months in the greenhouse, confirming their mature nature.

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Pour connaître la documentation sur le format Inist Standard.

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A02	`01`			`@0 PTCEDJ`
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A05				`@2 74`
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A08	`01`	`1`	`ENG`	`@1 Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments`
A11	`01`	`1`		`@1 KOBAYASHI (A. K.)`
A11	`02`	`1`		`@1 BESPALHOK (J. C.)`
A11	`03`	`1`		`@1 PEREIRA (L. F. P.)`
A11	`04`	`1`		`@1 VIEIRA (L. G. E.)`
A14	`01`			`@1 Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná (IAPAR), C.P. 481 @2 CEP 86001-970, Londrina, Paraná @3 BRA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.`
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A21				`@1 2003`
A23	`01`			`@0 ENG`
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A44				`@0 0000 @1 © 2003 INIST-CNRS. All rights reserved.`
A45				`@0 10 ref.`
A47	`01`	`1`		`@0 03-0479314`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Plant cell, tissue and organ culture`
A66	`01`			`@0 NLD`
C01	`01`		`ENG`	@0 An efficient system for in vitro plant regeneration from thin transversal stem sections explants (1-2 mm) using mature tissues of sweet orange cv. Pera was developed. Explants were cultured in different media to evaluate the frequency of regeneration and size of buds. A high percentage of explants (54% with 3.1 buds/explant) producing large buds (1-4 mm) was observed when the explants were cultivated for 2 weeks on Murashige and Skoog medium and then transferred to Woody Plant medium (WPM). Both media were supplemented with 1.8 μM 6-benzylaminopurine and 0.7 μM gibberellic acid. Adventitious buds were regenerated into whole plants by in vitro shoot-tip grafting. Regenerated plants started to flower after 12 months in the greenhouse, confirming their mature nature.
C02	`01`	`X`		`@0 002A`
N21				`@1 321`
N82				`@1 DST`

Format Inist (serveur)

NO :	PASCAL 03-0479314 INIST
ET :	Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments
AU :	KOBAYASHI (A. K.); BESPALHOK (J. C.); PEREIRA (L. F. P.); VIEIRA (L. G. E.)
AF :	Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná (IAPAR), C.P. 481/CEP 86001-970, Londrina, Paraná/Brésil (1 aut., 2 aut., 3 aut., 4 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant cell, tissue and organ culture; ISSN 0167-6857; Coden PTCEDJ; Pays-Bas; Da. 2003; Vol. 74; No. 1; Pp. 99-102; Bibl. 10 ref.
LA :	Anglais
EA :	An efficient system for in vitro plant regeneration from thin transversal stem sections explants (1-2 mm) using mature tissues of sweet orange cv. Pera was developed. Explants were cultured in different media to evaluate the frequency of regeneration and size of buds. A high percentage of explants (54% with 3.1 buds/explant) producing large buds (1-4 mm) was observed when the explants were cultivated for 2 weeks on Murashige and Skoog medium and then transferred to Woody Plant medium (WPM). Both media were supplemented with 1.8 μM 6-benzylaminopurine and 0.7 μM gibberellic acid. Adventitious buds were regenerated into whole plants by in vitro shoot-tip grafting. Regenerated plants started to flower after 12 months in the greenhouse, confirming their mature nature.
CC :	002A
LO :	INIST-19374.354000111089750110
ID :	03-0479314

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Pascal:03-0479314

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<AF>Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná (IAPAR), C.P. 481/CEP 86001-970, Londrina, Paraná/Brésil (1 aut., 2 aut., 3 aut., 4 aut.)</AF>
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Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Source :

Abstract

Notice en format standard (ISO 2709)

Format Inist (serveur)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri