OrangerV1, PascalFrancis, Corpus, bibRecord, 000696

A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri

Identifieur interne : 000696 ( PascalFrancis/Corpus ); précédent : 000695; suivant : 000697

A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri

Auteurs : Angela Mehta ; Yoko B. Rosato

Source :

Current microbiology : (Print) [ 0343-8651 ] ; 2003.

RBID : Pascal:04-0314193

Descripteurs français

Pascal (Inist)
- Xanthomonas axonopodis, Citrus sinensis, Méthode fingerprint, Phytopathogène, Protéine, RNA messager, Isolat, RNA 16S, RNA ribosomique, Réaction chaîne polymérase RT, Bactériose.

English descriptors

KwdEn :
- 16S-RNA, Bacteriosis, Citrus sinensis, Fingerprint method, Isolate, Messenger RNA, Plant pathogen, Protein, Reverse transcription polymerase chain reaction, Ribosomal RNA, Xanthomonas axonopodis.

Abstract

A major problem in studying bacterial plant pathogens is obtaining the microorganism directly from the plant tissue to perform in vivo expression (protein or mRNA) analyses. Here we report an easy and fast protocol to isolate Xanthomonas axonopodis pv. citri directly from the host plant, in sufficient amounts to perform protein fingerprinting by 2-D gel electrophoresis as well as RNA expression assays. The protein profile obtained was very similar to that of X. axonopodis pv. citri grown in the presence of a leaf extract of Citrus sinensis; however, some differential proteins expressed in vivo were observed. Total RNA extraction revealed typical 16S and 23S bands in the agarose gel, and RT-PCR reactions using primers specific for genes of the bacterium confirmed the quality of the RNA preparation. Also, RT-PCR reactions using plant ribosomal primers were employed, and no amplification product was obtained, indicating that plant RNA is not present in the bacterium RNA sample.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A08	`01`	`1`	`ENG`	`@1 A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri`
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A11	`02`	`1`		`@1 ROSATO (Yoko B.)`
A14	`01`			`@1 EMBRAPA-CENARGEN, Parque Estação Biológica Final Av. W/5 Norte @2 CEP 70770-900, Brasilia, DF @3 BRA @Z 1 aut.`
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A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
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C01	`01`		`ENG`	@0 A major problem in studying bacterial plant pathogens is obtaining the microorganism directly from the plant tissue to perform in vivo expression (protein or mRNA) analyses. Here we report an easy and fast protocol to isolate Xanthomonas axonopodis pv. citri directly from the host plant, in sufficient amounts to perform protein fingerprinting by 2-D gel electrophoresis as well as RNA expression assays. The protein profile obtained was very similar to that of X. axonopodis pv. citri grown in the presence of a leaf extract of Citrus sinensis; however, some differential proteins expressed in vivo were observed. Total RNA extraction revealed typical 16S and 23S bands in the agarose gel, and RT-PCR reactions using primers specific for genes of the bacterium confirmed the quality of the RNA preparation. Also, RT-PCR reactions using plant ribosomal primers were employed, and no amplification product was obtained, indicating that plant RNA is not present in the bacterium RNA sample.
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C03	`02`	`X`	`ENG`	`@0 Citrus sinensis @2 NS @5 03`
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C03	`05`	`X`	`ENG`	`@0 Protein @5 07`
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C03	`09`	`X`	`FRE`	`@0 RNA ribosomique @5 11`
C03	`09`	`X`	`ENG`	`@0 Ribosomal RNA @5 11`
C03	`09`	`X`	`SPA`	`@0 RNA ribosómico @5 11`
C03	`10`	`X`	`FRE`	`@0 Réaction chaîne polymérase RT @5 12`
C03	`10`	`X`	`ENG`	`@0 Reverse transcription polymerase chain reaction @5 12`
C03	`10`	`X`	`SPA`	`@0 Reacción cadena polimerasa transcripción inversa @5 12`
C03	`11`	`X`	`FRE`	`@0 Bactériose @2 NM @5 14`
C03	`11`	`X`	`ENG`	`@0 Bacteriosis @2 NM @5 14`
C03	`11`	`X`	`SPA`	`@0 Bacteriosis @2 NM @5 14`
C07	`01`	`X`	`FRE`	`@0 Pseudomonadaceae @2 NS`
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C07	`02`	`X`	`FRE`	`@0 Pseudomonadales @2 NS`
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C07	`03`	`X`	`FRE`	`@0 Bactérie`
C07	`03`	`X`	`ENG`	`@0 Bacteria`
C07	`03`	`X`	`SPA`	`@0 Bacteria`
C07	`04`	`X`	`FRE`	`@0 Rutaceae @2 NS`
C07	`04`	`X`	`ENG`	`@0 Rutaceae @2 NS`
C07	`04`	`X`	`SPA`	`@0 Rutaceae @2 NS`
C07	`05`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`05`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`05`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`06`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`06`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`06`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`07`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`07`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`07`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
C07	`08`	`X`	`FRE`	`@0 Infection @2 NM`
C07	`08`	`X`	`ENG`	`@0 Infection @2 NM`
C07	`08`	`X`	`SPA`	`@0 Infección @2 NM`
N21				`@1 187`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 04-0314193 INIST
ET :	A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri
AU :	MEHTA (Angela); ROSATO (Yoko B.)
AF :	EMBRAPA-CENARGEN, Parque Estação Biológica Final Av. W/5 Norte/CEP 70770-900, Brasilia, DF/Brésil (1 aut.); CBMEG-UNICAMP, Caixa Postal 6010/CEP 13083-970, Campinas, SP/Brésil (2 aut.)
DT :	Publication en série; Niveau analytique
SO :	Current microbiology : (Print); ISSN 0343-8651; Coden CUMIDD; Etats-Unis; Da. 2003; Vol. 47; No. 5; Pp. 400-403; Bibl. 18 ref.
LA :	Anglais
EA :	A major problem in studying bacterial plant pathogens is obtaining the microorganism directly from the plant tissue to perform in vivo expression (protein or mRNA) analyses. Here we report an easy and fast protocol to isolate Xanthomonas axonopodis pv. citri directly from the host plant, in sufficient amounts to perform protein fingerprinting by 2-D gel electrophoresis as well as RNA expression assays. The protein profile obtained was very similar to that of X. axonopodis pv. citri grown in the presence of a leaf extract of Citrus sinensis; however, some differential proteins expressed in vivo were observed. Total RNA extraction revealed typical 16S and 23S bands in the agarose gel, and RT-PCR reactions using primers specific for genes of the bacterium confirmed the quality of the RNA preparation. Also, RT-PCR reactions using plant ribosomal primers were employed, and no amplification product was obtained, indicating that plant RNA is not present in the bacterium RNA sample.
CC :	002A05C08; 002A05D07
FD :	Xanthomonas axonopodis; Citrus sinensis; Méthode fingerprint; Phytopathogène; Protéine; RNA messager; Isolat; RNA 16S; RNA ribosomique; Réaction chaîne polymérase RT; Bactériose
FG :	Pseudomonadaceae; Pseudomonadales; Bactérie; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Infection
ED :	Xanthomonas axonopodis; Citrus sinensis; Fingerprint method; Plant pathogen; Protein; Messenger RNA; Isolate; 16S-RNA; Ribosomal RNA; Reverse transcription polymerase chain reaction; Bacteriosis
EG :	Pseudomonadaceae; Pseudomonadales; Bacteria; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Infection
SD :	Xanthomonas axonopodis; Citrus sinensis; Método fingerprint; Fitopatógeno; Proteína; RNA mensajero; Aislado; ARN 16S; RNA ribosómico; Reacción cadena polimerasa transcripción inversa; Bacteriosis
LO :	INIST-17631.354000113389370090
ID :	04-0314193

Links to Exploration step

Pascal:04-0314193

Le document en format XML

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<front><div type="abstract" xml:lang="en">A major problem in studying bacterial plant pathogens is obtaining the microorganism directly from the plant tissue to perform in vivo expression (protein or mRNA) analyses. Here we report an easy and fast protocol to isolate Xanthomonas axonopodis pv. citri directly from the host plant, in sufficient amounts to perform protein fingerprinting by 2-D gel electrophoresis as well as RNA expression assays. The protein profile obtained was very similar to that of X. axonopodis pv. citri grown in the presence of a leaf extract of Citrus sinensis; however, some differential proteins expressed in vivo were observed. Total RNA extraction revealed typical 16S and 23S bands in the agarose gel, and RT-PCR reactions using primers specific for genes of the bacterium confirmed the quality of the RNA preparation. Also, RT-PCR reactions using plant ribosomal primers were employed, and no amplification product was obtained, indicating that plant RNA is not present in the bacterium RNA sample.</div>
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<fC03 i1="11" i2="X" l="ENG"><s0>Bacteriosis</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Bacteriosis</s0>
<s2>NM</s2>
<s5>14</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Infection</s0>
<s2>NM</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Infección</s0>
<s2>NM</s2>
</fC07>
<fN21><s1>187</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 04-0314193 INIST</NO>
<ET>A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri</ET>
<AU>MEHTA (Angela); ROSATO (Yoko B.)</AU>
<AF>EMBRAPA-CENARGEN, Parque Estação Biológica Final Av. W/5 Norte/CEP 70770-900, Brasilia, DF/Brésil (1 aut.); CBMEG-UNICAMP, Caixa Postal 6010/CEP 13083-970, Campinas, SP/Brésil (2 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Current microbiology : (Print); ISSN 0343-8651; Coden CUMIDD; Etats-Unis; Da. 2003; Vol. 47; No. 5; Pp. 400-403; Bibl. 18 ref.</SO>
<LA>Anglais</LA>
<EA>A major problem in studying bacterial plant pathogens is obtaining the microorganism directly from the plant tissue to perform in vivo expression (protein or mRNA) analyses. Here we report an easy and fast protocol to isolate Xanthomonas axonopodis pv. citri directly from the host plant, in sufficient amounts to perform protein fingerprinting by 2-D gel electrophoresis as well as RNA expression assays. The protein profile obtained was very similar to that of X. axonopodis pv. citri grown in the presence of a leaf extract of Citrus sinensis; however, some differential proteins expressed in vivo were observed. Total RNA extraction revealed typical 16S and 23S bands in the agarose gel, and RT-PCR reactions using primers specific for genes of the bacterium confirmed the quality of the RNA preparation. Also, RT-PCR reactions using plant ribosomal primers were employed, and no amplification product was obtained, indicating that plant RNA is not present in the bacterium RNA sample.</EA>
<CC>002A05C08; 002A05D07</CC>
<FD>Xanthomonas axonopodis; Citrus sinensis; Méthode fingerprint; Phytopathogène; Protéine; RNA messager; Isolat; RNA 16S; RNA ribosomique; Réaction chaîne polymérase RT; Bactériose</FD>
<FG>Pseudomonadaceae; Pseudomonadales; Bactérie; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Infection</FG>
<ED>Xanthomonas axonopodis; Citrus sinensis; Fingerprint method; Plant pathogen; Protein; Messenger RNA; Isolate; 16S-RNA; Ribosomal RNA; Reverse transcription polymerase chain reaction; Bacteriosis</ED>
<EG>Pseudomonadaceae; Pseudomonadales; Bacteria; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Infection</EG>
<SD>Xanthomonas axonopodis; Citrus sinensis; Método fingerprint; Fitopatógeno; Proteína; RNA mensajero; Aislado; ARN 16S; RNA ribosómico; Reacción cadena polimerasa transcripción inversa; Bacteriosis</SD>
<LO>INIST-17631.354000113389370090</LO>
<ID>04-0314193</ID>
</server>
</inist>
</record>

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A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri

A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri

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