OrangerV1, PascalFrancis, Corpus, bibRecord, 000695

Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas

Identifieur interne : 000695 ( PascalFrancis/Corpus ); précédent : 000694; suivant : 000696

Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas

Auteurs : Eliane Cristina Locali ; Juliana Freitas-Astua ; Alessandra Alves De Souza ; Marco Aurélio Takita ; Gustavo Astua-Monge ; Renata Antonioli ; Elliot W. Kitajima ; Marcos Antonio Machado

Source :

Plant disease [ 0191-2917 ] ; 2003.

RBID : Pascal:04-0316496

Descripteurs français

Pascal (Inist)
- Citrus, Diagnostic, Amérique, Développement, Microbiologie, Phytopathologie, Phytopathogène.

English descriptors

KwdEn :
- America, Citrus, Development, Diagnosis, Microbiology, Plant pathogen, Plant pathology.

Abstract

Citrus leprosis virus (CiLV), a tentative member of the Rhabdoviridae family, affects citrus trees in Brazil, where it is transmitted by mites Brevipalpus spp. It also occurs in other South American countries and was recently identified in Central America. This northbound spread of CiLV is being considered a serious threat to the citrus industry of the United States. However, despite its importance, difficulties related to the biology of CiLV have hindered much of the progress regarding its accurate detection, leaving both the analyses of symptoms and electron microscopy as the only tools available. An attempt to overcome this problem was made by constructing a cDNA library from double-stranded RNA extracted from leprosis lesions of infected Citrus sinensis (sweet orange) leaves. After cloning and sequencing, specific primers were designed to amplify putative CiLV genome regions with similarity to genes encoding the movement protein and replicase of other plant viruses. RNA from infected citrus plants corresponding to different varieties and locations were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the two pairs of primers. Amplified products were purified, cloned in pGEM-T, and sequenced. The sequences confirmed the genomic regions previously associated with CiLV. The results demonstrate that RT-PCR was specific, accurate, rapid, and reliable for the detection of CiLV.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0191-2917`
A02	`01`			`@0 PLDIDE`
A03		`1`		`@0 Plant dis.`
A05				`@2 87`
A06				`@2 11`
A08	`01`	`1`	`ENG`	`@1 Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas`
A11	`01`	`1`		`@1 LOCALI (Eliane Cristina)`
A11	`02`	`1`		`@1 FREITAS-ASTUA (Juliana)`
A11	`03`	`1`		`@1 ALVES DE SOUZA (Alessandra)`
A11	`04`	`1`		`@1 TAKITA (Marco Aurélio)`
A11	`05`	`1`		`@1 ASTUA-MONGE (Gustavo)`
A11	`06`	`1`		`@1 ANTONIOLI (Renata)`
A11	`07`	`1`		`@1 KITAJIMA (Elliot W.)`
A11	`08`	`1`		`@1 MACHADO (Marcos Antonio)`
A14	`01`			`@1 Centro APTA Citros ' Sylvio Moreira'/IAC, Rod. Anhanguera Km 158, CP 4 @2 13490-970, Cordeirópolis-SP @3 BRA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut. @Z 8 aut.`
A14	`02`			`@1 Empresa Brasileira de Pesquisa Agropecuária @3 BRA @Z 2 aut. @Z 3 aut.`
A14	`03`			`@1 Depto. Entomol., Fitopatol. & Zool. Agríc., Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de Sao Paulo, CP 9 @2 13418-900, Piracicaba, SP @3 BRA @Z 7 aut.`
A20				`@1 1317-1321`
A21				`@1 2003`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 12673 @5 354000113364280070`
A44				`@0 0000 @1 © 2004 INIST-CNRS. All rights reserved.`
A45				`@0 28 ref.`
A47	`01`	`1`		`@0 04-0316496`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Plant disease`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Citrus leprosis virus (CiLV), a tentative member of the Rhabdoviridae family, affects citrus trees in Brazil, where it is transmitted by mites Brevipalpus spp. It also occurs in other South American countries and was recently identified in Central America. This northbound spread of CiLV is being considered a serious threat to the citrus industry of the United States. However, despite its importance, difficulties related to the biology of CiLV have hindered much of the progress regarding its accurate detection, leaving both the analyses of symptoms and electron microscopy as the only tools available. An attempt to overcome this problem was made by constructing a cDNA library from double-stranded RNA extracted from leprosis lesions of infected Citrus sinensis (sweet orange) leaves. After cloning and sequencing, specific primers were designed to amplify putative CiLV genome regions with similarity to genes encoding the movement protein and replicase of other plant viruses. RNA from infected citrus plants corresponding to different varieties and locations were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the two pairs of primers. Amplified products were purified, cloned in pGEM-T, and sequenced. The sequences confirmed the genomic regions previously associated with CiLV. The results demonstrate that RT-PCR was specific, accurate, rapid, and reliable for the detection of CiLV.
C02	`01`	`X`		`@0 002A34`
C03	`01`	`X`	`FRE`	`@0 Citrus @2 NS @5 01`
C03	`01`	`X`	`ENG`	`@0 Citrus @2 NS @5 01`
C03	`01`	`X`	`SPA`	`@0 Citrus @2 NS @5 01`
C03	`02`	`X`	`FRE`	`@0 Diagnostic @5 05`
C03	`02`	`X`	`ENG`	`@0 Diagnosis @5 05`
C03	`02`	`X`	`SPA`	`@0 Diagnóstico @5 05`
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C03	`03`	`X`	`ENG`	`@0 America @2 NG @5 06`
C03	`03`	`X`	`SPA`	`@0 America @2 NG @5 06`
C03	`04`	`X`	`FRE`	`@0 Développement @5 07`
C03	`04`	`X`	`ENG`	`@0 Development @5 07`
C03	`04`	`X`	`SPA`	`@0 Desarrollo @5 07`
C03	`05`	`X`	`FRE`	`@0 Microbiologie @5 08`
C03	`05`	`X`	`ENG`	`@0 Microbiology @5 08`
C03	`05`	`X`	`SPA`	`@0 Microbiología @5 08`
C03	`06`	`X`	`FRE`	`@0 Phytopathologie @5 09`
C03	`06`	`X`	`ENG`	`@0 Plant pathology @5 09`
C03	`06`	`X`	`SPA`	`@0 Fitopatología @5 09`
C03	`07`	`X`	`FRE`	`@0 Phytopathogène @5 10`
C03	`07`	`X`	`ENG`	`@0 Plant pathogen @5 10`
C03	`07`	`X`	`SPA`	`@0 Fitopatógeno @5 10`
C07	`01`	`X`	`FRE`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`ENG`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`SPA`	`@0 Rutaceae @2 NS`
C07	`02`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`03`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
N21				`@1 187`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 04-0316496 INIST
ET :	Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas
AU :	LOCALI (Eliane Cristina); FREITAS-ASTUA (Juliana); ALVES DE SOUZA (Alessandra); TAKITA (Marco Aurélio); ASTUA-MONGE (Gustavo); ANTONIOLI (Renata); KITAJIMA (Elliot W.); MACHADO (Marcos Antonio)
AF :	Centro APTA Citros ' Sylvio Moreira'/IAC, Rod. Anhanguera Km 158, CP 4/13490-970, Cordeirópolis-SP/Brésil (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 8 aut.); Empresa Brasileira de Pesquisa Agropecuária/Brésil (2 aut., 3 aut.); Depto. Entomol., Fitopatol. & Zool. Agríc., Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de Sao Paulo, CP 9/13418-900, Piracicaba, SP/Brésil (7 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant disease; ISSN 0191-2917; Coden PLDIDE; Etats-Unis; Da. 2003; Vol. 87; No. 11; Pp. 1317-1321; Bibl. 28 ref.
LA :	Anglais
EA :	Citrus leprosis virus (CiLV), a tentative member of the Rhabdoviridae family, affects citrus trees in Brazil, where it is transmitted by mites Brevipalpus spp. It also occurs in other South American countries and was recently identified in Central America. This northbound spread of CiLV is being considered a serious threat to the citrus industry of the United States. However, despite its importance, difficulties related to the biology of CiLV have hindered much of the progress regarding its accurate detection, leaving both the analyses of symptoms and electron microscopy as the only tools available. An attempt to overcome this problem was made by constructing a cDNA library from double-stranded RNA extracted from leprosis lesions of infected Citrus sinensis (sweet orange) leaves. After cloning and sequencing, specific primers were designed to amplify putative CiLV genome regions with similarity to genes encoding the movement protein and replicase of other plant viruses. RNA from infected citrus plants corresponding to different varieties and locations were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the two pairs of primers. Amplified products were purified, cloned in pGEM-T, and sequenced. The sequences confirmed the genomic regions previously associated with CiLV. The results demonstrate that RT-PCR was specific, accurate, rapid, and reliable for the detection of CiLV.
CC :	002A34
FD :	Citrus; Diagnostic; Amérique; Développement; Microbiologie; Phytopathologie; Phytopathogène
FG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
ED :	Citrus; Diagnosis; America; Development; Microbiology; Plant pathology; Plant pathogen
EG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
SD :	Citrus; Diagnóstico; America; Desarrollo; Microbiología; Fitopatología; Fitopatógeno
LO :	INIST-12673.354000113364280070
ID :	04-0316496

Links to Exploration step

Pascal:04-0316496

Le document en format XML

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<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>America</term>
<term>Citrus</term>
<term>Development</term>
<term>Diagnosis</term>
<term>Microbiology</term>
<term>Plant pathogen</term>
<term>Plant pathology</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Citrus</term>
<term>Diagnostic</term>
<term>Amérique</term>
<term>Développement</term>
<term>Microbiologie</term>
<term>Phytopathologie</term>
<term>Phytopathogène</term>
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<front><div type="abstract" xml:lang="en">Citrus leprosis virus (CiLV), a tentative member of the Rhabdoviridae family, affects citrus trees in Brazil, where it is transmitted by mites Brevipalpus spp. It also occurs in other South American countries and was recently identified in Central America. This northbound spread of CiLV is being considered a serious threat to the citrus industry of the United States. However, despite its importance, difficulties related to the biology of CiLV have hindered much of the progress regarding its accurate detection, leaving both the analyses of symptoms and electron microscopy as the only tools available. An attempt to overcome this problem was made by constructing a cDNA library from double-stranded RNA extracted from leprosis lesions of infected Citrus sinensis (sweet orange) leaves. After cloning and sequencing, specific primers were designed to amplify putative CiLV genome regions with similarity to genes encoding the movement protein and replicase of other plant viruses. RNA from infected citrus plants corresponding to different varieties and locations were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the two pairs of primers. Amplified products were purified, cloned in pGEM-T, and sequenced. The sequences confirmed the genomic regions previously associated with CiLV. The results demonstrate that RT-PCR was specific, accurate, rapid, and reliable for the detection of CiLV.</div>
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<fA02 i1="01"><s0>PLDIDE</s0>
</fA02>
<fA03 i2="1"><s0>Plant dis.</s0>
</fA03>
<fA05><s2>87</s2>
</fA05>
<fA06><s2>11</s2>
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<fA08 i1="01" i2="1" l="ENG"><s1>Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LOCALI (Eliane Cristina)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>FREITAS-ASTUA (Juliana)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>ALVES DE SOUZA (Alessandra)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>TAKITA (Marco Aurélio)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>ASTUA-MONGE (Gustavo)</s1>
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<fA11 i1="06" i2="1"><s1>ANTONIOLI (Renata)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>KITAJIMA (Elliot W.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>MACHADO (Marcos Antonio)</s1>
</fA11>
<fA14 i1="01"><s1>Centro APTA Citros ' Sylvio Moreira'/IAC, Rod. Anhanguera Km 158, CP 4</s1>
<s2>13490-970, Cordeirópolis-SP</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Empresa Brasileira de Pesquisa Agropecuária</s1>
<s3>BRA</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Depto. Entomol., Fitopatol. & Zool. Agríc., Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de Sao Paulo, CP 9</s1>
<s2>13418-900, Piracicaba, SP</s2>
<s3>BRA</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>1317-1321</s1>
</fA20>
<fA21><s1>2003</s1>
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<s2>12673</s2>
<s5>354000113364280070</s5>
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<fA44><s0>0000</s0>
<s1>© 2004 INIST-CNRS. All rights reserved.</s1>
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<fC01 i1="01" l="ENG"><s0>Citrus leprosis virus (CiLV), a tentative member of the Rhabdoviridae family, affects citrus trees in Brazil, where it is transmitted by mites Brevipalpus spp. It also occurs in other South American countries and was recently identified in Central America. This northbound spread of CiLV is being considered a serious threat to the citrus industry of the United States. However, despite its importance, difficulties related to the biology of CiLV have hindered much of the progress regarding its accurate detection, leaving both the analyses of symptoms and electron microscopy as the only tools available. An attempt to overcome this problem was made by constructing a cDNA library from double-stranded RNA extracted from leprosis lesions of infected Citrus sinensis (sweet orange) leaves. After cloning and sequencing, specific primers were designed to amplify putative CiLV genome regions with similarity to genes encoding the movement protein and replicase of other plant viruses. RNA from infected citrus plants corresponding to different varieties and locations were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the two pairs of primers. Amplified products were purified, cloned in pGEM-T, and sequenced. The sequences confirmed the genomic regions previously associated with CiLV. The results demonstrate that RT-PCR was specific, accurate, rapid, and reliable for the detection of CiLV.</s0>
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<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Citrus</s0>
<s2>NS</s2>
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<s5>05</s5>
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<s5>07</s5>
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<s5>07</s5>
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<s5>08</s5>
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<s5>09</s5>
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<s5>10</s5>
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<s5>10</s5>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<fN44 i1="01"><s1>OTO</s1>
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<fN82><s1>OTO</s1>
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<server><NO>PASCAL 04-0316496 INIST</NO>
<ET>Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas</ET>
<AU>LOCALI (Eliane Cristina); FREITAS-ASTUA (Juliana); ALVES DE SOUZA (Alessandra); TAKITA (Marco Aurélio); ASTUA-MONGE (Gustavo); ANTONIOLI (Renata); KITAJIMA (Elliot W.); MACHADO (Marcos Antonio)</AU>
<AF>Centro APTA Citros ' Sylvio Moreira'/IAC, Rod. Anhanguera Km 158, CP 4/13490-970, Cordeirópolis-SP/Brésil (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut., 8 aut.); Empresa Brasileira de Pesquisa Agropecuária/Brésil (2 aut., 3 aut.); Depto. Entomol., Fitopatol. & Zool. Agríc., Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de Sao Paulo, CP 9/13418-900, Piracicaba, SP/Brésil (7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant disease; ISSN 0191-2917; Coden PLDIDE; Etats-Unis; Da. 2003; Vol. 87; No. 11; Pp. 1317-1321; Bibl. 28 ref.</SO>
<LA>Anglais</LA>
<EA>Citrus leprosis virus (CiLV), a tentative member of the Rhabdoviridae family, affects citrus trees in Brazil, where it is transmitted by mites Brevipalpus spp. It also occurs in other South American countries and was recently identified in Central America. This northbound spread of CiLV is being considered a serious threat to the citrus industry of the United States. However, despite its importance, difficulties related to the biology of CiLV have hindered much of the progress regarding its accurate detection, leaving both the analyses of symptoms and electron microscopy as the only tools available. An attempt to overcome this problem was made by constructing a cDNA library from double-stranded RNA extracted from leprosis lesions of infected Citrus sinensis (sweet orange) leaves. After cloning and sequencing, specific primers were designed to amplify putative CiLV genome regions with similarity to genes encoding the movement protein and replicase of other plant viruses. RNA from infected citrus plants corresponding to different varieties and locations were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the two pairs of primers. Amplified products were purified, cloned in pGEM-T, and sequenced. The sequences confirmed the genomic regions previously associated with CiLV. The results demonstrate that RT-PCR was specific, accurate, rapid, and reliable for the detection of CiLV.</EA>
<CC>002A34</CC>
<FD>Citrus; Diagnostic; Amérique; Développement; Microbiologie; Phytopathologie; Phytopathogène</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Citrus; Diagnosis; America; Development; Microbiology; Plant pathology; Plant pathogen</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Citrus; Diagnóstico; America; Desarrollo; Microbiología; Fitopatología; Fitopatógeno</SD>
<LO>INIST-12673.354000113364280070</LO>
<ID>04-0316496</ID>
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Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas

Development of a molecular tool for the diagnosis of leprosis, a major threat to citrus production in the Americas

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