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Characterization of electrical penetration graphs of Bucephalogonia xanthophis, a vector of Xylella fastidiosa in citrus

Identifieur interne : 000285 ( PascalFrancis/Corpus ); précédent : 000284; suivant : 000286

Characterization of electrical penetration graphs of Bucephalogonia xanthophis, a vector of Xylella fastidiosa in citrus

Auteurs : M. P. Miranda ; A. Fereres ; B. Appezzato-Da-Gloria ; J. R. S. Lopes

Source :

RBID : Francis:10-0022871

Descripteurs français

English descriptors

Abstract

The sharpshooter Bucephalogonia xanthophis (Berg) (Homoptera: Cicadellidae) is a vector of the xylem-limited bacterium, Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner), which causes citrus variegated chlorosis. Despite the importance of citrus variegated chlorosis, the probing behavior of vectors on citrus and its implications for transmission ofX. fastidiosa have not been studied. Here we studied electrical penetration graph (EPG-DC system) waveforms produced by B. xanthophis on Citrus sinensis (L.) Osbeck (Rutaceae), and their relationships with stylet activities and xylem ingestion. Electrical penetration graph waveforms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration on plant tissues. The main waveforms were correlated with histological observations of salivary sheaths in plant tissues and excretion analysis, in order to determine stylet activities and their precise position. Six waveforms and associated activities are described: (S) secretion of salivary sheath and intracellular stylet pathway, (R) resting during stylet pathway, (Xc) contact of stylets with xylem vessels, (Xi) active xylem ingestion, (N) interruption within the xylem phase (during Xc or Xi), and (W) withdrawal of stylet from the plant. The sharpshooter spent 91.8% of its probing time with its stylet in the xylem, where the main activity was ingestion (Xi: 97.5%). During a probe, the most likely sequence of events is secretion of salivary sheath and pathway (S) through epidermal and parenchyma cells (all individuals), followed by contact with xylem (Xc) (67.6% of all individuals) and ingestion (Xi) (88.3% of those that exhibit waveform Xc). The mean time to contact the xylem (Xc) and initiate ingestion (Xi) after onset of the first probe was 27.8 and 34.2 min, respectively. However, sustained xylem ingestion (Xi > 5 min) was established after 39.8 min, on average. This information is basic for future studies on the transmission mechanisms of X. fastidiosa and in order to establish control strategies aimed at interfering with this process.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A02 01      @0 ETEAAT
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A05       @2 130
A06       @2 1
A08 01  1  ENG  @1 Characterization of electrical penetration graphs of Bucephalogonia xanthophis, a vector of Xylella fastidiosa in citrus
A11 01  1    @1 MIRANDA (M. P.)
A11 02  1    @1 FERERES (A.)
A11 03  1    @1 APPEZZATO-DA-GLORIA (B.)
A11 04  1    @1 LOPES (J. R. S.)
A14 01      @1 Departamento de Entomologia, Fitopatologia e Zoologia Agricola, ESALQ/Universidade de Sào Paulo, CP. 9 @2 Piracicaba, SP 13418-900 @3 BRA @Z 1 aut. @Z 4 aut.
A14 02      @1 Departamento de Protección Végétal, Instituto de Ciencias Agrarias (ICA, CSIC), C/Serrano, 115 dpdo @2 28006 Madrid @3 ESP @Z 2 aut.
A14 03      @1 Departamento de Ciências Biológicas, ESALQ/Universidade de São Paulo, CP. 9 @2 Piracicaba, SP 13418-900 @3 BRA @Z 3 aut.
A20       @1 35-46
A21       @1 2009
A23 01      @0 ENG
A43 01      @1 INIST @2 8629 @5 354000185055460050
A44       @0 0000 @1 © 2010 INIST-CNRS. All rights reserved.
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C01 01    ENG  @0 The sharpshooter Bucephalogonia xanthophis (Berg) (Homoptera: Cicadellidae) is a vector of the xylem-limited bacterium, Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner), which causes citrus variegated chlorosis. Despite the importance of citrus variegated chlorosis, the probing behavior of vectors on citrus and its implications for transmission ofX. fastidiosa have not been studied. Here we studied electrical penetration graph (EPG-DC system) waveforms produced by B. xanthophis on Citrus sinensis (L.) Osbeck (Rutaceae), and their relationships with stylet activities and xylem ingestion. Electrical penetration graph waveforms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration on plant tissues. The main waveforms were correlated with histological observations of salivary sheaths in plant tissues and excretion analysis, in order to determine stylet activities and their precise position. Six waveforms and associated activities are described: (S) secretion of salivary sheath and intracellular stylet pathway, (R) resting during stylet pathway, (Xc) contact of stylets with xylem vessels, (Xi) active xylem ingestion, (N) interruption within the xylem phase (during Xc or Xi), and (W) withdrawal of stylet from the plant. The sharpshooter spent 91.8% of its probing time with its stylet in the xylem, where the main activity was ingestion (Xi: 97.5%). During a probe, the most likely sequence of events is secretion of salivary sheath and pathway (S) through epidermal and parenchyma cells (all individuals), followed by contact with xylem (Xc) (67.6% of all individuals) and ingestion (Xi) (88.3% of those that exhibit waveform Xc). The mean time to contact the xylem (Xc) and initiate ingestion (Xi) after onset of the first probe was 27.8 and 34.2 min, respectively. However, sustained xylem ingestion (Xi > 5 min) was established after 39.8 min, on average. This information is basic for future studies on the transmission mechanisms of X. fastidiosa and in order to establish control strategies aimed at interfering with this process.
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Format Inist (serveur)

NO : FRANCIS 10-0022871 INIST
ET : Characterization of electrical penetration graphs of Bucephalogonia xanthophis, a vector of Xylella fastidiosa in citrus
AU : MIRANDA (M. P.); FERERES (A.); APPEZZATO-DA-GLORIA (B.); LOPES (J. R. S.)
AF : Departamento de Entomologia, Fitopatologia e Zoologia Agricola, ESALQ/Universidade de Sào Paulo, CP. 9/Piracicaba, SP 13418-900/Brésil (1 aut., 4 aut.); Departamento de Protección Végétal, Instituto de Ciencias Agrarias (ICA, CSIC), C/Serrano, 115 dpdo/28006 Madrid/Espagne (2 aut.); Departamento de Ciências Biológicas, ESALQ/Universidade de São Paulo, CP. 9/Piracicaba, SP 13418-900/Brésil (3 aut.)
DT : Publication en série; Niveau analytique
SO : Entomologia experimentalis et applicata; ISSN 0013-8703; Coden ETEAAT; Royaume-Uni; Da. 2009; Vol. 130; No. 1; Pp. 35-46; Bibl. 1 p.1/2
LA : Anglais
EA : The sharpshooter Bucephalogonia xanthophis (Berg) (Homoptera: Cicadellidae) is a vector of the xylem-limited bacterium, Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner), which causes citrus variegated chlorosis. Despite the importance of citrus variegated chlorosis, the probing behavior of vectors on citrus and its implications for transmission ofX. fastidiosa have not been studied. Here we studied electrical penetration graph (EPG-DC system) waveforms produced by B. xanthophis on Citrus sinensis (L.) Osbeck (Rutaceae), and their relationships with stylet activities and xylem ingestion. Electrical penetration graph waveforms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration on plant tissues. The main waveforms were correlated with histological observations of salivary sheaths in plant tissues and excretion analysis, in order to determine stylet activities and their precise position. Six waveforms and associated activities are described: (S) secretion of salivary sheath and intracellular stylet pathway, (R) resting during stylet pathway, (Xc) contact of stylets with xylem vessels, (Xi) active xylem ingestion, (N) interruption within the xylem phase (during Xc or Xi), and (W) withdrawal of stylet from the plant. The sharpshooter spent 91.8% of its probing time with its stylet in the xylem, where the main activity was ingestion (Xi: 97.5%). During a probe, the most likely sequence of events is secretion of salivary sheath and pathway (S) through epidermal and parenchyma cells (all individuals), followed by contact with xylem (Xc) (67.6% of all individuals) and ingestion (Xi) (88.3% of those that exhibit waveform Xc). The mean time to contact the xylem (Xc) and initiate ingestion (Xi) after onset of the first probe was 27.8 and 34.2 min, respectively. However, sustained xylem ingestion (Xi > 5 min) was established after 39.8 min, on average. This information is basic for future studies on the transmission mechanisms of X. fastidiosa and in order to establish control strategies aimed at interfering with this process.
CC : 770A02
FD : Comportement alimentaire; Déprédateur; Arbre fruitier; Vecteur; Bactérie; Phytopathogène; Histologie; Relation animal végétal; Citrus sinensis; Cicadellidae; Bucephalogonia xanthophis; Electropénétrographe
FG : Agrume; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Cicadomorpha; Homoptera; Insecta; Arthropoda; Invertebrata
ED : Feeding behavior; Pest; Fruit tree; Vector; Bacteria; Plant pathogen; Histology; Animal plant relation; Citrus sinensis; Cicadellidae; Electric penetration graph
EG : Citrus fruit; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Cicadomorpha; Homoptera; Insecta; Arthropoda; Invertebrata
SD : Conducta alimenticia; Plaga; Arbol frutal; Vector; Bacteria; Fitopatógeno; Histología; Relación animal vegetal; Citrus sinensis; Cicadellidae; Penetrógrafo eléctrico
LO : INIST-8629.354000185055460050
ID : 10-0022871

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Francis:10-0022871

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<s1>Departamento de Ciências Biológicas, ESALQ/Universidade de São Paulo, CP. 9</s1>
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<s3>BRA</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA20>
<s1>35-46</s1>
</fA20>
<fA21>
<s1>2009</s1>
</fA21>
<fA23 i1="01">
<s0>ENG</s0>
</fA23>
<fA43 i1="01">
<s1>INIST</s1>
<s2>8629</s2>
<s5>354000185055460050</s5>
</fA43>
<fA44>
<s0>0000</s0>
<s1>© 2010 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45>
<s0>1 p.1/2</s0>
</fA45>
<fA47 i1="01" i2="1">
<s0>10-0022871</s0>
</fA47>
<fA60>
<s1>P</s1>
</fA60>
<fA61>
<s0>A</s0>
</fA61>
<fA64 i1="01" i2="1">
<s0>Entomologia experimentalis et applicata</s0>
</fA64>
<fA66 i1="01">
<s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG">
<s0>The sharpshooter Bucephalogonia xanthophis (Berg) (Homoptera: Cicadellidae) is a vector of the xylem-limited bacterium, Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner), which causes citrus variegated chlorosis. Despite the importance of citrus variegated chlorosis, the probing behavior of vectors on citrus and its implications for transmission ofX. fastidiosa have not been studied. Here we studied electrical penetration graph (EPG-DC system) waveforms produced by B. xanthophis on Citrus sinensis (L.) Osbeck (Rutaceae), and their relationships with stylet activities and xylem ingestion. Electrical penetration graph waveforms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration on plant tissues. The main waveforms were correlated with histological observations of salivary sheaths in plant tissues and excretion analysis, in order to determine stylet activities and their precise position. Six waveforms and associated activities are described: (S) secretion of salivary sheath and intracellular stylet pathway, (R) resting during stylet pathway, (Xc) contact of stylets with xylem vessels, (Xi) active xylem ingestion, (N) interruption within the xylem phase (during Xc or Xi), and (W) withdrawal of stylet from the plant. The sharpshooter spent 91.8% of its probing time with its stylet in the xylem, where the main activity was ingestion (Xi: 97.5%). During a probe, the most likely sequence of events is secretion of salivary sheath and pathway (S) through epidermal and parenchyma cells (all individuals), followed by contact with xylem (Xc) (67.6% of all individuals) and ingestion (Xi) (88.3% of those that exhibit waveform Xc). The mean time to contact the xylem (Xc) and initiate ingestion (Xi) after onset of the first probe was 27.8 and 34.2 min, respectively. However, sustained xylem ingestion (Xi > 5 min) was established after 39.8 min, on average. This information is basic for future studies on the transmission mechanisms of X. fastidiosa and in order to establish control strategies aimed at interfering with this process.</s0>
</fC01>
<fC02 i1="01" i2="X">
<s0>770A02</s0>
<s1>I</s1>
</fC02>
<fC03 i1="01" i2="X" l="FRE">
<s0>Comportement alimentaire</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG">
<s0>Feeding behavior</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA">
<s0>Conducta alimenticia</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Déprédateur</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Pest</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Plaga</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Arbre fruitier</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Fruit tree</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Arbol frutal</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Vecteur</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Vector</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Vector</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Bactérie</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Bacteria</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Bacteria</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Phytopathogène</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>Plant pathogen</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>Fitopatógeno</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE">
<s0>Histologie</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Histology</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
<s0>Histología</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE">
<s0>Relation animal végétal</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG">
<s0>Animal plant relation</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
<s0>Relación animal vegetal</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE">
<s0>Cicadellidae</s0>
<s2>NS</s2>
<s5>57</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG">
<s0>Cicadellidae</s0>
<s2>NS</s2>
<s5>57</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA">
<s0>Cicadellidae</s0>
<s2>NS</s2>
<s5>57</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE">
<s0>Bucephalogonia xanthophis</s0>
<s4>INC</s4>
<s5>87</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE">
<s0>Electropénétrographe</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG">
<s0>Electric penetration graph</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA">
<s0>Penetrógrafo eléctrico</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Agrume</s0>
<s5>26</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Citrus fruit</s0>
<s5>26</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Agrios</s0>
<s5>26</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Cicadomorpha</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Cicadomorpha</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA">
<s0>Cicadomorpha</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE">
<s0>Homoptera</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG">
<s0>Homoptera</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA">
<s0>Homoptera</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE">
<s0>Insecta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG">
<s0>Insecta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA">
<s0>Insecta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE">
<s0>Arthropoda</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="ENG">
<s0>Arthropoda</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="SPA">
<s0>Arthropoda</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="10" i2="X" l="FRE">
<s0>Invertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="10" i2="X" l="ENG">
<s0>Invertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="10" i2="X" l="SPA">
<s0>Invertebrata</s0>
<s2>NS</s2>
</fC07>
<fN21>
<s1>011</s1>
</fN21>
</pA>
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<server>
<NO>FRANCIS 10-0022871 INIST</NO>
<ET>Characterization of electrical penetration graphs of Bucephalogonia xanthophis, a vector of Xylella fastidiosa in citrus</ET>
<AU>MIRANDA (M. P.); FERERES (A.); APPEZZATO-DA-GLORIA (B.); LOPES (J. R. S.)</AU>
<AF>Departamento de Entomologia, Fitopatologia e Zoologia Agricola, ESALQ/Universidade de Sào Paulo, CP. 9/Piracicaba, SP 13418-900/Brésil (1 aut., 4 aut.); Departamento de Protección Végétal, Instituto de Ciencias Agrarias (ICA, CSIC), C/Serrano, 115 dpdo/28006 Madrid/Espagne (2 aut.); Departamento de Ciências Biológicas, ESALQ/Universidade de São Paulo, CP. 9/Piracicaba, SP 13418-900/Brésil (3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Entomologia experimentalis et applicata; ISSN 0013-8703; Coden ETEAAT; Royaume-Uni; Da. 2009; Vol. 130; No. 1; Pp. 35-46; Bibl. 1 p.1/2</SO>
<LA>Anglais</LA>
<EA>The sharpshooter Bucephalogonia xanthophis (Berg) (Homoptera: Cicadellidae) is a vector of the xylem-limited bacterium, Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner), which causes citrus variegated chlorosis. Despite the importance of citrus variegated chlorosis, the probing behavior of vectors on citrus and its implications for transmission ofX. fastidiosa have not been studied. Here we studied electrical penetration graph (EPG-DC system) waveforms produced by B. xanthophis on Citrus sinensis (L.) Osbeck (Rutaceae), and their relationships with stylet activities and xylem ingestion. Electrical penetration graph waveforms were described based on amplitude, frequency, voltage level, and electrical origin of the observed traces during stylet penetration on plant tissues. The main waveforms were correlated with histological observations of salivary sheaths in plant tissues and excretion analysis, in order to determine stylet activities and their precise position. Six waveforms and associated activities are described: (S) secretion of salivary sheath and intracellular stylet pathway, (R) resting during stylet pathway, (Xc) contact of stylets with xylem vessels, (Xi) active xylem ingestion, (N) interruption within the xylem phase (during Xc or Xi), and (W) withdrawal of stylet from the plant. The sharpshooter spent 91.8% of its probing time with its stylet in the xylem, where the main activity was ingestion (Xi: 97.5%). During a probe, the most likely sequence of events is secretion of salivary sheath and pathway (S) through epidermal and parenchyma cells (all individuals), followed by contact with xylem (Xc) (67.6% of all individuals) and ingestion (Xi) (88.3% of those that exhibit waveform Xc). The mean time to contact the xylem (Xc) and initiate ingestion (Xi) after onset of the first probe was 27.8 and 34.2 min, respectively. However, sustained xylem ingestion (Xi > 5 min) was established after 39.8 min, on average. This information is basic for future studies on the transmission mechanisms of X. fastidiosa and in order to establish control strategies aimed at interfering with this process.</EA>
<CC>770A02</CC>
<FD>Comportement alimentaire; Déprédateur; Arbre fruitier; Vecteur; Bactérie; Phytopathogène; Histologie; Relation animal végétal; Citrus sinensis; Cicadellidae; Bucephalogonia xanthophis; Electropénétrographe</FD>
<FG>Agrume; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Cicadomorpha; Homoptera; Insecta; Arthropoda; Invertebrata</FG>
<ED>Feeding behavior; Pest; Fruit tree; Vector; Bacteria; Plant pathogen; Histology; Animal plant relation; Citrus sinensis; Cicadellidae; Electric penetration graph</ED>
<EG>Citrus fruit; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Cicadomorpha; Homoptera; Insecta; Arthropoda; Invertebrata</EG>
<SD>Conducta alimenticia; Plaga; Arbol frutal; Vector; Bacteria; Fitopatógeno; Histología; Relación animal vegetal; Citrus sinensis; Cicadellidae; Penetrógrafo eléctrico</SD>
<LO>INIST-8629.354000185055460050</LO>
<ID>10-0022871</ID>
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