OrangerV1, PascalFrancis, Corpus, bibRecord, 000018

Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay

Identifieur interne : 000018 ( PascalFrancis/Corpus ); précédent : 000017; suivant : 000019

Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay

Auteurs : Miriam Aldeguer ; Maria Lopez-Andreo ; José A. Gabaldon ; Antonio Puyet

Source :

Food chemistry [ 0308-8146 ] ; 2014.

RBID : Pascal:14-0123351

Descripteurs français

Pascal (Inist)
- Détection, Jus d'orange, Nucléotide, Polymorphisme, Dosage, Identification, Aliment, Qualité, Temps réel, Réaction chaîne polymérase, Sonde, Adultération, Citrus.

English descriptors

KwdEn :
- Adulteration, Assay, Citrus, Detection, Food, Identification, Nucleotide, Orange juice, Polymerase chain reaction, Polymorphism, Probe, Quality, Real time.

Abstract

A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin x tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C_T bias allowed accurate quantitative measurements to be obtained.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`01`	`1`		`@1 ALDEGUER (Miriam)`
A11	`02`	`1`		`@1 LOPEZ-ANDREO (Maria)`
A11	`03`	`1`		`@1 GABALDON (José A.)`
A11	`04`	`1`		`@1 PUYET (Antonio)`
A14	`01`			`@1 Centro Tecnológico Nacional de la Conserva @2 Molina de Segura, Murcia @3 ESP @Z 1 aut. @Z 3 aut.`
A14	`02`			`@1 Departamento de Bioquímica y Biologia Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid @2 28040 Madrid @3 ESP @Z 2 aut. @Z 4 aut.`
A14	`03`			`@1 Departamento de Ciencia y Tecnología de Alimentos, Universidad Católica San Antonio de Murcia (UCAM) @2 Murcia @3 ESP @Z 1 aut. @Z 3 aut.`
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A43	`01`			`@1 INIST @2 17810 @5 354000506188351490`
A44				`@0 0000 @1 © 2014 INIST-CNRS. All rights reserved.`
A45				`@0 3/4 p.`
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A64	`01`	`1`		`@0 Food chemistry`
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C01	`01`		`ENG`	@0 A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin x tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C_T bias allowed accurate quantitative measurements to be obtained.
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C03	`02`	`X`	`ENG`	`@0 Orange juice @5 02`
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C03	`03`	`X`	`FRE`	`@0 Nucléotide @5 03`
C03	`03`	`X`	`ENG`	`@0 Nucleotide @5 03`
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C03	`04`	`X`	`ENG`	`@0 Polymorphism @5 04`
C03	`04`	`X`	`SPA`	`@0 Polimorfismo @5 04`
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C03	`08`	`X`	`FRE`	`@0 Qualité @5 14`
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C07	`01`	`X`	`SPA`	`@0 Rutaceae @2 NS`
C07	`02`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
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C07	`03`	`X`	`FRE`	`@0 Angiospermae @2 NS`
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C07	`03`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
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N21				`@1 160`
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N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 14-0123351 INIST
ET :	Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay
AU :	ALDEGUER (Miriam); LOPEZ-ANDREO (Maria); GABALDON (José A.); PUYET (Antonio)
AF :	Centro Tecnológico Nacional de la Conserva/Molina de Segura, Murcia/Espagne (1 aut., 3 aut.); Departamento de Bioquímica y Biologia Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid/28040 Madrid/Espagne (2 aut., 4 aut.); Departamento de Ciencia y Tecnología de Alimentos, Universidad Católica San Antonio de Murcia (UCAM)/Murcia/Espagne (1 aut., 3 aut.)
DT :	Publication en série; Niveau analytique
SO :	Food chemistry; ISSN 0308-8146; Coden FOCHDJ; Royaume-Uni; Da. 2014; Vol. 145; Pp. 1086-1091; Bibl. 3/4 p.
LA :	Anglais
EA :	A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin x tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C_T bias allowed accurate quantitative measurements to be obtained.
CC :	002B03H
FD :	Détection; Jus d'orange; Nucléotide; Polymorphisme; Dosage; Identification; Aliment; Qualité; Temps réel; Réaction chaîne polymérase; Sonde; Adultération; Citrus
FG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
ED :	Detection; Orange juice; Nucleotide; Polymorphism; Assay; Identification; Food; Quality; Real time; Polymerase chain reaction; Probe; Adulteration; Citrus
EG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
SD :	Detección; Zugo naranja; Nucleótido; Polimorfismo; Dosificación; Identificación; Alimento; Calidad; Tiempo real; Reacción cadena polimerasa; Sonda; Adulteración; Citrus
LO :	INIST-17810.354000506188351490
ID :	14-0123351

Links to Exploration step

Pascal:14-0123351

Le document en format XML

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<front><div type="abstract" xml:lang="en">A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin x tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C<sub>T</sub>
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<fC01 i1="01" l="ENG"><s0>A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin x tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C<sub>T</sub>
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<fC03 i1="01" i2="X" l="SPA"><s0>Detección</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Jus d'orange</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Orange juice</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Zugo naranja</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Nucléotide</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Nucleotide</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Nucleótido</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Polymorphisme</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Polymorphism</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Polimorfismo</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Dosage</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Assay</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Dosificación</s0>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Identification</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Identification</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Identificación</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Aliment</s0>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Food</s0>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Alimento</s0>
<s5>13</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Qualité</s0>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Quality</s0>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Calidad</s0>
<s5>14</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Temps réel</s0>
<s5>15</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Real time</s0>
<s5>15</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Tiempo real</s0>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Réaction chaîne polymérase</s0>
<s5>16</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Polymerase chain reaction</s0>
<s5>16</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Reacción cadena polimerasa</s0>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Sonde</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Probe</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Sonda</s0>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Adultération</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Adulteration</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Adulteración</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Citrus</s0>
<s2>NS</s2>
<s5>69</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Citrus</s0>
<s2>NS</s2>
<s5>69</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Citrus</s0>
<s2>NS</s2>
<s5>69</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>160</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 14-0123351 INIST</NO>
<ET>Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay</ET>
<AU>ALDEGUER (Miriam); LOPEZ-ANDREO (Maria); GABALDON (José A.); PUYET (Antonio)</AU>
<AF>Centro Tecnológico Nacional de la Conserva/Molina de Segura, Murcia/Espagne (1 aut., 3 aut.); Departamento de Bioquímica y Biologia Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid/28040 Madrid/Espagne (2 aut., 4 aut.); Departamento de Ciencia y Tecnología de Alimentos, Universidad Católica San Antonio de Murcia (UCAM)/Murcia/Espagne (1 aut., 3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Food chemistry; ISSN 0308-8146; Coden FOCHDJ; Royaume-Uni; Da. 2014; Vol. 145; Pp. 1086-1091; Bibl. 3/4 p.</SO>
<LA>Anglais</LA>
<EA>A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin x tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C<sub>T</sub>
 bias allowed accurate quantitative measurements to be obtained.</EA>
<CC>002B03H</CC>
<FD>Détection; Jus d'orange; Nucléotide; Polymorphisme; Dosage; Identification; Aliment; Qualité; Temps réel; Réaction chaîne polymérase; Sonde; Adultération; Citrus</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Detection; Orange juice; Nucleotide; Polymorphism; Assay; Identification; Food; Quality; Real time; Polymerase chain reaction; Probe; Adulteration; Citrus</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Detección; Zugo naranja; Nucleótido; Polimorfismo; Dosificación; Identificación; Alimento; Calidad; Tiempo real; Reacción cadena polimerasa; Sonda; Adulteración; Citrus</SD>
<LO>INIST-17810.354000506188351490</LO>
<ID>14-0123351</ID>
</server>
</inist>
</record>

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Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay

Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay

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