OrangerV1, PascalFrancis, Checkpoint, bibRecord, 000433

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Identifieur interne : 000433 ( PascalFrancis/Checkpoint ); précédent : 000432; suivant : 000434

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Auteurs : A. A. Omar [États-Unis] ; J. W. Grosser [États-Unis]

Source :

Acta horticulturae [ 0567-7572 ] ; 2007.

RBID : Pascal:07-0538144

Descripteurs français

Pascal (Inist)
- Plante transgénique, Cultivar, Bactériose, Résistance microorganisme, Gène indicateur, Gène structure, Multigène, Protoplaste, Régénération, Transformation génétique, Citrus sinensis, Oryza sativa, Xanthomonas axonopodis, DNA complémentaire, Plasmide, Protéine fluorescente verte, Culture cellulaire, Système combiné, cv Hamlin, Gène gfp, Gène Xa21, Xanthomonas oryzae, Chancre plante.
Wicri :
- topic : Plante transgénique.

English descriptors

KwdEn :
- Bacteriosis, Cell culture, Citrus sinensis, Combined system, Complementary DNA, Cultivar, Genetic transformation, Green fluorescent protein, Microorganism resistance, Multigene family, Plant cankers, Protoplast, Regeneration, Reporter gene, Structural gene, Transgenic plant, Xanthomonas axonopodis.

Abstract

[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]

Affiliations:

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Le document en format XML

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<s3>USA</s3>
<sZ>1 aut.</sZ>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP</title>
<author><name sortKey="Omar, A A" sort="Omar, A A" uniqKey="Omar A" first="A. A." last="Omar">A. A. Omar</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>University of Florida Citrus Research and Education Center</s1>
<s2>Lake Alfred, FL</s2>
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<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>University of Florida Citrus Research and Education Center</s1>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriosis</term>
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<term>Combined system</term>
<term>Complementary DNA</term>
<term>Cultivar</term>
<term>Genetic transformation</term>
<term>Green fluorescent protein</term>
<term>Microorganism resistance</term>
<term>Multigene family</term>
<term>Plant cankers</term>
<term>Protoplast</term>
<term>Regeneration</term>
<term>Reporter gene</term>
<term>Structural gene</term>
<term>Transgenic plant</term>
<term>Xanthomonas axonopodis</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Plante transgénique</term>
<term>Cultivar</term>
<term>Bactériose</term>
<term>Résistance microorganisme</term>
<term>Gène indicateur</term>
<term>Gène structure</term>
<term>Multigène</term>
<term>Protoplaste</term>
<term>Régénération</term>
<term>Transformation génétique</term>
<term>Citrus sinensis</term>
<term>Oryza sativa</term>
<term>Xanthomonas axonopodis</term>
<term>DNA complémentaire</term>
<term>Plasmide</term>
<term>Protéine fluorescente verte</term>
<term>Culture cellulaire</term>
<term>Système combiné</term>
<term>cv Hamlin</term>
<term>Gène gfp</term>
<term>Gène Xa21</term>
<term>Xanthomonas oryzae</term>
<term>Chancre plante</term>
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<front><div type="abstract" xml:lang="en">[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]</div>
</front>
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<fA08 i1="01" i2="1" l="ENG"><s1>Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP</s1>
</fA08>
<fA09 i1="01" i2="1" l="ENG"><s1>Proceedings of the International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species : Daytona Beach, Florida, USA, October 10-14, 2005</s1>
</fA09>
<fA11 i1="01" i2="1"><s1>OMAR (A. A.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>GROSSER (J. W.)</s1>
</fA11>
<fA12 i1="01" i2="1"><s1>LITZ (Richard E.)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="02" i2="1"><s1>SCORZA (Ralph)</s1>
<s9>ed.</s9>
</fA12>
<fA14 i1="01"><s1>University of Florida Citrus Research and Education Center</s1>
<s2>Lake Alfred, FL</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</fA14>
<fA18 i1="01" i2="1"><s1>International Society for Horticultural Science. Section on Tropical and Subtropical Fruits</s1>
<s3>INT</s3>
<s9>org-cong.</s9>
</fA18>
<fA20><s1>235-243</s1>
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<fA21><s1>2007</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA26 i1="01"><s0>978-90-6605-219-2</s0>
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<fA43 i1="01"><s1>INIST</s1>
<s2>15963</s2>
<s5>354000153599050230</s5>
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<fA44><s0>0000</s0>
<s1>© 2007 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>1 p.1/2</s0>
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<fA60><s1>P</s1>
<s2>C</s2>
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<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Acta horticulturae</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31C02A5B</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A31C07C3</s0>
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<fC02 i1="03" i2="X"><s0>002A32D02B</s0>
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<fC02 i1="04" i2="X"><s0>002A32D03B2</s0>
</fC02>
<fC02 i1="05" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Plante transgénique</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Transgenic plant</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Planta transgénica</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Cultivar</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Cultivar</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Cultivar</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Bactériose</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Bacteriosis</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Bacteriosis</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Résistance microorganisme</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Microorganism resistance</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Resistencia microorganismo</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Gène indicateur</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Reporter gene</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Gen marcador</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Gène structure</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Structural gene</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Gen estructura</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Multigène</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Multigene family</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Multigén</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Protoplaste</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Protoplast</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Protoplasto</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Régénération</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Regeneration</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Regeneración</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Transformation génétique</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Genetic transformation</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Transformación genética</s0>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Oryza sativa</s0>
<s4>INC</s4>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Xanthomonas axonopodis</s0>
<s2>NS</s2>
<s5>14</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Xanthomonas axonopodis</s0>
<s2>NS</s2>
<s5>14</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Xanthomonas axonopodis</s0>
<s2>NS</s2>
<s5>14</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>DNA complémentaire</s0>
<s5>15</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Complementary DNA</s0>
<s5>15</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>DNA complementario</s0>
<s5>15</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Plasmide</s0>
<s4>INC</s4>
<s5>16</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Protéine fluorescente verte</s0>
<s5>17</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Green fluorescent protein</s0>
<s5>17</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Proteína fluorescente verde</s0>
<s5>17</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Culture cellulaire</s0>
<s5>20</s5>
</fC03>
<fC03 i1="17" i2="X" l="ENG"><s0>Cell culture</s0>
<s5>20</s5>
</fC03>
<fC03 i1="17" i2="X" l="SPA"><s0>Cultivo celular</s0>
<s5>20</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Système combiné</s0>
<s5>21</s5>
</fC03>
<fC03 i1="18" i2="X" l="ENG"><s0>Combined system</s0>
<s5>21</s5>
</fC03>
<fC03 i1="18" i2="X" l="SPA"><s0>Sistema combinado</s0>
<s5>21</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>cv Hamlin</s0>
<s4>INC</s4>
<s5>68</s5>
</fC03>
<fC03 i1="20" i2="X" l="FRE"><s0>Gène gfp</s0>
<s4>INC</s4>
<s5>69</s5>
</fC03>
<fC03 i1="21" i2="X" l="FRE"><s0>Gène Xa21</s0>
<s4>INC</s4>
<s5>70</s5>
</fC03>
<fC03 i1="22" i2="X" l="FRE"><s0>Xanthomonas oryzae</s0>
<s4>INC</s4>
<s5>71</s5>
</fC03>
<fC03 i1="23" i2="X" l="FRE"><s0>Chancre plante</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="23" i2="X" l="ENG"><s0>Plant cankers</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="23" i2="X" l="SPA"><s0>Cancro</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Agrume</s0>
<s4>INC</s4>
<s5>31</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Arbre fruitier</s0>
<s4>INC</s4>
<s5>32</s5>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Organisme génétiquement modifié</s0>
<s4>INC</s4>
<s5>33</s5>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Plante fruitière</s0>
<s5>34</s5>
</fC07>
<fC07 i1="12" i2="X" l="ENG"><s0>Fruit crop</s0>
<s5>34</s5>
</fC07>
<fC07 i1="12" i2="X" l="SPA"><s0>Planta frutal</s0>
<s5>34</s5>
</fC07>
<fC07 i1="13" i2="X" l="FRE"><s0>Phytopathogène</s0>
<s5>35</s5>
</fC07>
<fC07 i1="13" i2="X" l="ENG"><s0>Plant pathogen</s0>
<s5>35</s5>
</fC07>
<fC07 i1="13" i2="X" l="SPA"><s0>Fitopatógeno</s0>
<s5>35</s5>
</fC07>
<fC07 i1="14" i2="X" l="FRE"><s0>Sensibilité résistance</s0>
<s4>INC</s4>
<s5>36</s5>
</fC07>
<fC07 i1="15" i2="X" l="FRE"><s0>Amélioration génétique</s0>
<s5>39</s5>
</fC07>
<fC07 i1="15" i2="X" l="ENG"><s0>Genetic improvement</s0>
<s5>39</s5>
</fC07>
<fC07 i1="15" i2="X" l="SPA"><s0>Enriquecimiento genético</s0>
<s5>39</s5>
</fC07>
<fC07 i1="16" i2="X" l="FRE"><s0>Biotechnologie</s0>
<s5>40</s5>
</fC07>
<fC07 i1="16" i2="X" l="ENG"><s0>Biotechnology</s0>
<s5>40</s5>
</fC07>
<fC07 i1="16" i2="X" l="SPA"><s0>Biotecnología</s0>
<s5>40</s5>
</fC07>
<fC07 i1="17" i2="X" l="FRE"><s0>Génie génétique</s0>
<s5>41</s5>
</fC07>
<fC07 i1="17" i2="X" l="ENG"><s0>Genetic engineering</s0>
<s5>41</s5>
</fC07>
<fC07 i1="17" i2="X" l="SPA"><s0>Ingeniería genética</s0>
<s5>41</s5>
</fC07>
<fC07 i1="18" i2="X" l="FRE"><s0>Plante ligneuse</s0>
<s4>INC</s4>
<s5>42</s5>
</fC07>
<fC07 i1="19" i2="X" l="FRE"><s0>Acide nucléique</s0>
<s4>INC</s4>
<s5>43</s5>
</fC07>
<fC07 i1="20" i2="X" l="FRE"><s0>Composé biochimique</s0>
<s4>INC</s4>
<s5>44</s5>
</fC07>
<fC07 i1="21" i2="X" l="FRE"><s0>Gène</s0>
<s4>INC</s4>
<s5>45</s5>
</fC07>
<fC07 i1="22" i2="X" l="FRE"><s0>Arboriculture</s0>
<s5>64</s5>
</fC07>
<fC07 i1="22" i2="X" l="ENG"><s0>Arboriculture</s0>
<s5>64</s5>
</fC07>
<fC07 i1="22" i2="X" l="SPA"><s0>Arboricultura</s0>
<s5>64</s5>
</fC07>
<fC07 i1="23" i2="X" l="FRE"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="23" i2="X" l="ENG"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="23" i2="X" l="SPA"><s0>Horticultura</s0>
<s5>65</s5>
</fC07>
<fC07 i1="24" i2="X" l="FRE"><s0>Industrie fruits</s0>
<s5>66</s5>
</fC07>
<fC07 i1="24" i2="X" l="ENG"><s0>Fruit industry</s0>
<s5>66</s5>
</fC07>
<fC07 i1="24" i2="X" l="SPA"><s0>Industria frutas</s0>
<s5>66</s5>
</fC07>
<fN21><s1>351</s1>
</fN21>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species</s1>
<s3>Daytona Beach FL USA</s3>
<s4>2005</s4>
</fA30>
</pR>
</standard>
</inist>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Floride</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Floride"><name sortKey="Omar, A A" sort="Omar, A A" uniqKey="Omar A" first="A. A." last="Omar">A. A. Omar</name>
</region>
<name sortKey="Grosser, J W" sort="Grosser, J W" uniqKey="Grosser J" first="J. W." last="Grosser">J. W. Grosser</name>
</country>
</tree>
</affiliations>
</record>

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   |texte=   Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP
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Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

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