Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils
Identifieur interne : 002012 ( Ncbi/Merge ); précédent : 002011; suivant : 002013Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils
Auteurs : Nancy J. Ruiz-Pérez ; Marisela González-Ávila ; Jaime Sánchez-Navarrete ; Julia D. Toscano-Garibay ; Mario A. Moreno-Eutimio ; Teresa Sandoval-Hernández ; Myriam Arriaga-AlbaSource :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of
Url:
DOI: 10.1038/srep25371
PubMed: 27137128
PubMed Central: 4853807
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antimycotic Activity and Genotoxic Evaluation of <italic>Citrus sinensis</italic>
and <italic>Citrus latifolia</italic>
Essential Oils</title>
<author><name sortKey="Ruiz Perez, Nancy J" sort="Ruiz Perez, Nancy J" uniqKey="Ruiz Perez N" first="Nancy J." last="Ruiz-Pérez">Nancy J. Ruiz-Pérez</name>
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<author><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name>
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<author><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Antimycotic Activity and Genotoxic Evaluation of <italic>Citrus sinensis</italic>
and <italic>Citrus latifolia</italic>
Essential Oils</title>
<author><name sortKey="Ruiz Perez, Nancy J" sort="Ruiz Perez, Nancy J" uniqKey="Ruiz Perez N" first="Nancy J." last="Ruiz-Pérez">Nancy J. Ruiz-Pérez</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name>
<affiliation><nlm:aff id="a2"><institution>Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Av. Normalistas # 800 Col. Colinas de la Normal</institution>
, Guadalajara, Jal. México C.P. 44270</nlm:aff>
<wicri:noCountry code="subfield">Jal. México C.P. 44270</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Toscano Garibay, Julia D" sort="Toscano Garibay, Julia D" uniqKey="Toscano Garibay J" first="Julia D." last="Toscano-Garibay">Julia D. Toscano-Garibay</name>
<affiliation><nlm:aff id="a3"><institution>Laboratorio de Medicina Regenerativa, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Moreno Eutimio, Mario A" sort="Moreno Eutimio, Mario A" uniqKey="Moreno Eutimio M" first="Mario A." last="Moreno-Eutimio">Mario A. Moreno-Eutimio</name>
<affiliation><nlm:aff id="a4"><institution>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650.</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sandoval Hernandez, Teresa" sort="Sandoval Hernandez, Teresa" uniqKey="Sandoval Hernandez T" first="Teresa" last="Sandoval-Hernández">Teresa Sandoval-Hernández</name>
<affiliation><nlm:aff id="a4"><institution>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650.</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Arriaga Alba, Myriam" sort="Arriaga Alba, Myriam" uniqKey="Arriaga Alba M" first="Myriam" last="Arriaga-Alba">Myriam Arriaga-Alba</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2016">2016</date>
</imprint>
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<front><div type="abstract" xml:lang="en"><p>The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of <italic>Citrus sinensis</italic>
(<italic>C. sinensis</italic>
) and <italic>Citrus latifolia</italic>
(<italic>C. latifolia</italic>
) against five <italic>Candida</italic>
species: <italic>Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae</italic>
and <italic>Candida guilliermondii</italic>
; and perform its genotoxic evaluation. The EOs of <italic>C. sinensis</italic>
and <italic>C. latifolia</italic>
were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in <italic>C. sinensis</italic>
, d-limonene (96%) and α-myrcene (2.79%); and in <italic>C. latifolia</italic>
, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where <italic>C. sinensis</italic>
presented low activity and <italic>C. latifolia</italic>
essential oil was effective to inhibit growing of <italic>C. lusitaniae</italic>
and <italic>C. guilliermondii</italic>
with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for <italic>C. sinensis</italic>
were in a range of 0.42–3.71 μg and for <italic>C. latifolia</italic>
of 0.22–1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, <italic>C. sinensis</italic>
was not cytotoxic and <italic>C. latifolia</italic>
was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil.</p>
</div>
</front>
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</TEI>
<double pmid="27137128"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antimycotic Activity and Genotoxic Evaluation of <italic>Citrus sinensis</italic>
and <italic>Citrus latifolia</italic>
Essential Oils</title>
<author><name sortKey="Ruiz Perez, Nancy J" sort="Ruiz Perez, Nancy J" uniqKey="Ruiz Perez N" first="Nancy J." last="Ruiz-Pérez">Nancy J. Ruiz-Pérez</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name>
<affiliation><nlm:aff id="a2"><institution>Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Av. Normalistas # 800 Col. Colinas de la Normal</institution>
, Guadalajara, Jal. México C.P. 44270</nlm:aff>
<wicri:noCountry code="subfield">Jal. México C.P. 44270</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Toscano Garibay, Julia D" sort="Toscano Garibay, Julia D" uniqKey="Toscano Garibay J" first="Julia D." last="Toscano-Garibay">Julia D. Toscano-Garibay</name>
<affiliation><nlm:aff id="a3"><institution>Laboratorio de Medicina Regenerativa, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Moreno Eutimio, Mario A" sort="Moreno Eutimio, Mario A" uniqKey="Moreno Eutimio M" first="Mario A." last="Moreno-Eutimio">Mario A. Moreno-Eutimio</name>
<affiliation><nlm:aff id="a4"><institution>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650.</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sandoval Hernandez, Teresa" sort="Sandoval Hernandez, Teresa" uniqKey="Sandoval Hernandez T" first="Teresa" last="Sandoval-Hernández">Teresa Sandoval-Hernández</name>
<affiliation><nlm:aff id="a4"><institution>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650.</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Arriaga Alba, Myriam" sort="Arriaga Alba, Myriam" uniqKey="Arriaga Alba M" first="Myriam" last="Arriaga-Alba">Myriam Arriaga-Alba</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27137128</idno>
<idno type="pmc">4853807</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853807</idno>
<idno type="RBID">PMC:4853807</idno>
<idno type="doi">10.1038/srep25371</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000463</idno>
<idno type="wicri:Area/Pmc/Curation">000462</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000263</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Antimycotic Activity and Genotoxic Evaluation of <italic>Citrus sinensis</italic>
and <italic>Citrus latifolia</italic>
Essential Oils</title>
<author><name sortKey="Ruiz Perez, Nancy J" sort="Ruiz Perez, Nancy J" uniqKey="Ruiz Perez N" first="Nancy J." last="Ruiz-Pérez">Nancy J. Ruiz-Pérez</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name>
<affiliation><nlm:aff id="a2"><institution>Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Av. Normalistas # 800 Col. Colinas de la Normal</institution>
, Guadalajara, Jal. México C.P. 44270</nlm:aff>
<wicri:noCountry code="subfield">Jal. México C.P. 44270</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Toscano Garibay, Julia D" sort="Toscano Garibay, Julia D" uniqKey="Toscano Garibay J" first="Julia D." last="Toscano-Garibay">Julia D. Toscano-Garibay</name>
<affiliation><nlm:aff id="a3"><institution>Laboratorio de Medicina Regenerativa, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Moreno Eutimio, Mario A" sort="Moreno Eutimio, Mario A" uniqKey="Moreno Eutimio M" first="Mario A." last="Moreno-Eutimio">Mario A. Moreno-Eutimio</name>
<affiliation><nlm:aff id="a4"><institution>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650.</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sandoval Hernandez, Teresa" sort="Sandoval Hernandez, Teresa" uniqKey="Sandoval Hernandez T" first="Teresa" last="Sandoval-Hernández">Teresa Sandoval-Hernández</name>
<affiliation><nlm:aff id="a4"><institution>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650.</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Arriaga Alba, Myriam" sort="Arriaga Alba, Myriam" uniqKey="Arriaga Alba M" first="Myriam" last="Arriaga-Alba">Myriam Arriaga-Alba</name>
<affiliation><nlm:aff id="a1"><institution>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas</institution>
, México D.F. C.P. 07650</nlm:aff>
<wicri:noCountry code="subfield">México D.F. C.P. 07650</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of <italic>Citrus sinensis</italic>
(<italic>C. sinensis</italic>
) and <italic>Citrus latifolia</italic>
(<italic>C. latifolia</italic>
) against five <italic>Candida</italic>
species: <italic>Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae</italic>
and <italic>Candida guilliermondii</italic>
; and perform its genotoxic evaluation. The EOs of <italic>C. sinensis</italic>
and <italic>C. latifolia</italic>
were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in <italic>C. sinensis</italic>
, d-limonene (96%) and α-myrcene (2.79%); and in <italic>C. latifolia</italic>
, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where <italic>C. sinensis</italic>
presented low activity and <italic>C. latifolia</italic>
essential oil was effective to inhibit growing of <italic>C. lusitaniae</italic>
and <italic>C. guilliermondii</italic>
with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for <italic>C. sinensis</italic>
were in a range of 0.42–3.71 μg and for <italic>C. latifolia</italic>
of 0.22–1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, <italic>C. sinensis</italic>
was not cytotoxic and <italic>C. latifolia</italic>
was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil.</p>
</div>
</front>
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<author><name sortKey="Miyaji, M" uniqKey="Miyaji M">M. Miyaji</name>
</author>
<author><name sortKey="Kurane, R" uniqKey="Kurane R">R. Kurane</name>
</author>
<author><name sortKey="Takahara, Y" uniqKey="Takahara Y">Y. Takahara</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Filtenborg, O" uniqKey="Filtenborg O">O. Filtenborg</name>
</author>
<author><name sortKey="Frisvad, J C" uniqKey="Frisvad J">J. C. Frisvad</name>
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<author><name sortKey="Thrane, U" uniqKey="Thrane U">U. Thrane</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lota, M L" uniqKey="Lota M">M. L. Lota</name>
</author>
<author><name sortKey="De Rocca Serra, D" uniqKey="De Rocca Serra D">D. de Rocca Serra</name>
</author>
<author><name sortKey="Tomi, F" uniqKey="Tomi F">F. Tomi</name>
</author>
<author><name sortKey="Jacquemond, C" uniqKey="Jacquemond C">C. Jacquemond</name>
</author>
<author><name sortKey="Casanova, J" uniqKey="Casanova J">J. Casanova</name>
</author>
</analytic>
</biblStruct>
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</TEI>
</pmc>
<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils.</title>
<author><name sortKey="Ruiz Perez, Nancy J" sort="Ruiz Perez, Nancy J" uniqKey="Ruiz Perez N" first="Nancy J" last="Ruiz-Pérez">Nancy J. Ruiz-Pérez</name>
<affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name>
<affiliation><nlm:affiliation>Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Av. Normalistas # 800 Col. Colinas de la Normal, Guadalajara, Jal. México C.P. 44270.</nlm:affiliation>
<wicri:noCountry code="subField">Jal</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name>
<affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Toscano Garibay, Julia D" sort="Toscano Garibay, Julia D" uniqKey="Toscano Garibay J" first="Julia D" last="Toscano-Garibay">Julia D. Toscano-Garibay</name>
<affiliation><nlm:affiliation>Laboratorio de Medicina Regenerativa, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Moreno Eutimio, Mario A" sort="Moreno Eutimio, Mario A" uniqKey="Moreno Eutimio M" first="Mario A" last="Moreno-Eutimio">Mario A. Moreno-Eutimio</name>
<affiliation><nlm:affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sandoval Hernandez, Teresa" sort="Sandoval Hernandez, Teresa" uniqKey="Sandoval Hernandez T" first="Teresa" last="Sandoval-Hernández">Teresa Sandoval-Hernández</name>
<affiliation><nlm:affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Arriaga Alba, Myriam" sort="Arriaga Alba, Myriam" uniqKey="Arriaga Alba M" first="Myriam" last="Arriaga-Alba">Myriam Arriaga-Alba</name>
<affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:27137128</idno>
<idno type="pmid">27137128</idno>
<idno type="doi">10.1038/srep25371</idno>
<idno type="wicri:Area/PubMed/Corpus">000048</idno>
<idno type="wicri:Area/PubMed/Curation">000048</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000048</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils.</title>
<author><name sortKey="Ruiz Perez, Nancy J" sort="Ruiz Perez, Nancy J" uniqKey="Ruiz Perez N" first="Nancy J" last="Ruiz-Pérez">Nancy J. Ruiz-Pérez</name>
<affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name>
<affiliation><nlm:affiliation>Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Av. Normalistas # 800 Col. Colinas de la Normal, Guadalajara, Jal. México C.P. 44270.</nlm:affiliation>
<wicri:noCountry code="subField">Jal</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name>
<affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Toscano Garibay, Julia D" sort="Toscano Garibay, Julia D" uniqKey="Toscano Garibay J" first="Julia D" last="Toscano-Garibay">Julia D. Toscano-Garibay</name>
<affiliation><nlm:affiliation>Laboratorio de Medicina Regenerativa, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Moreno Eutimio, Mario A" sort="Moreno Eutimio, Mario A" uniqKey="Moreno Eutimio M" first="Mario A" last="Moreno-Eutimio">Mario A. Moreno-Eutimio</name>
<affiliation><nlm:affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Sandoval Hernandez, Teresa" sort="Sandoval Hernandez, Teresa" uniqKey="Sandoval Hernandez T" first="Teresa" last="Sandoval-Hernández">Teresa Sandoval-Hernández</name>
<affiliation><nlm:affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Arriaga Alba, Myriam" sort="Arriaga Alba, Myriam" uniqKey="Arriaga Alba M" first="Myriam" last="Arriaga-Alba">Myriam Arriaga-Alba</name>
<affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation>
<wicri:noCountry code="subField">México D.F.</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
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<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of Citrus sinensis (C. sinensis) and Citrus latifolia (C. latifolia) against five Candida species: Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae and Candida guilliermondii; and perform its genotoxic evaluation. The EOs of C. sinensis and C. latifolia were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in C. sinensis, d-limonene (96%) and α-myrcene (2.79%); and in C. latifolia, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where C. sinensis presented low activity and C. latifolia essential oil was effective to inhibit growing of C. lusitaniae and C. guilliermondii with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for C. sinensis were in a range of 0.42-3.71 μg and for C. latifolia of 0.22-1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, C. sinensis was not cytotoxic and C. latifolia was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil.</div>
</front>
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