Possible Involvement of Locus-Specific Methylation on Expression Regulation of LEAFY Homologous Gene (CiLFY) during Precocious Trifoliate Orange Phase Change Process
Identifieur interne : 001597 ( Ncbi/Merge ); précédent : 001596; suivant : 001598Possible Involvement of Locus-Specific Methylation on Expression Regulation of LEAFY Homologous Gene (CiLFY) during Precocious Trifoliate Orange Phase Change Process
Auteurs : Jin-Zhi Zhang ; Li Mei ; Rong Liu ; Muhammad Rehman Gul Khan ; Chun-Gen HuSource :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
DNA methylation plays an essential role in regulating plant development. Here, we described an early flowering trifoliate orange (precocious trifoliate orange,
Url:
DOI: 10.1371/journal.pone.0088558
PubMed: 24523915
PubMed Central: 3921215
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001191
- to stream Pmc, to step Curation: 001190
- to stream Pmc, to step Checkpoint: 000734
Links to Exploration step
PMC:3921215Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Possible Involvement of Locus-Specific Methylation on Expression Regulation of <italic>LEAFY</italic> Homologous Gene (<italic>CiLFY</italic>) during Precocious Trifoliate Orange Phase Change Process</title><author><name sortKey="Zhang, Jin Zhi" sort="Zhang, Jin Zhi" uniqKey="Zhang J" first="Jin-Zhi" last="Zhang">Jin-Zhi Zhang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mei, Li" sort="Mei, Li" uniqKey="Mei L" first="Li" last="Mei">Li Mei</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Liu, Rong" sort="Liu, Rong" uniqKey="Liu R" first="Rong" last="Liu">Rong Liu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Khan, Muhammad Rehman Gul" sort="Khan, Muhammad Rehman Gul" uniqKey="Khan M" first="Muhammad Rehman Gul" last="Khan">Muhammad Rehman Gul Khan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Chun Gen" sort="Hu, Chun Gen" uniqKey="Hu C" first="Chun-Gen" last="Hu">Chun-Gen Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24523915</idno><idno type="pmc">3921215</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3921215</idno><idno type="RBID">PMC:3921215</idno><idno type="doi">10.1371/journal.pone.0088558</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">001191</idno><idno type="wicri:Area/Pmc/Curation">001190</idno><idno type="wicri:Area/Pmc/Checkpoint">000734</idno><idno type="wicri:Area/Ncbi/Merge">001597</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Possible Involvement of Locus-Specific Methylation on Expression Regulation of <italic>LEAFY</italic> Homologous Gene (<italic>CiLFY</italic>) during Precocious Trifoliate Orange Phase Change Process</title><author><name sortKey="Zhang, Jin Zhi" sort="Zhang, Jin Zhi" uniqKey="Zhang J" first="Jin-Zhi" last="Zhang">Jin-Zhi Zhang</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mei, Li" sort="Mei, Li" uniqKey="Mei L" first="Li" last="Mei">Li Mei</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Liu, Rong" sort="Liu, Rong" uniqKey="Liu R" first="Rong" last="Liu">Rong Liu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Khan, Muhammad Rehman Gul" sort="Khan, Muhammad Rehman Gul" uniqKey="Khan M" first="Muhammad Rehman Gul" last="Khan">Muhammad Rehman Gul Khan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Chun Gen" sort="Hu, Chun Gen" uniqKey="Hu C" first="Chun-Gen" last="Hu">Chun-Gen Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>DNA methylation plays an essential role in regulating plant development. Here, we described an early flowering trifoliate orange (precocious trifoliate orange, <italic>Poncirus trifoliata</italic> L. Raf) was treated with 5-azacytidine and displayed a number of phenotypic and developmental abnormalities. These observations suggested that DNA methylation might play an important role in regulating many developmental pathways including early flowering trait, and then the expression level of five key or integrated citrus flowering genes were analyzed. Our results showed that <italic>FLOWERING LOCUS T</italic> (<italic>CiFT</italic>) relative expression level was increased with the increasing concentrations of 5-AzaC. However, <italic>LEAFY</italic> (<italic>CiLFY</italic>), <italic>APETELA1</italic> (<italic>CiAP1</italic>), <italic>TERMINAL FLOWER1</italic> (<italic>CiTFL1</italic>), and <italic>FLOWERING LOCUS C</italic> (<italic>CiFLC</italic>) showed highest relative expression levels at 250 µΜ treatment, while decreased sharply at higher concentrations. In order to further confirm DNA methylation affects the expression of these genes, their full-length sequences were isolated by genome-walker method, and then was analyzed by using bioinformatics tools. However, only one locus-specific methylation site was observed in <italic>CiLFY</italic> sequence. Therefore, DNA methylation level of the <italic>CiLFY</italic> was investigated both at juvenile and adult stages of precocious trifoliate orange by bisulfate sequencing PCR; it has been shown that the level of DNA methylation was altered during phase change. In addition, spatial and temporal expression patterns of <italic>CiLFY</italic> promoter and a series of 5′ deletions were investigated by driving the expression of a β-glucuronidase reporter gene in <italic>Arabidopsis</italic>. Exogenous GA<sub>3</sub> treatment on transgenic <italic>Arabidopsis</italic> revealed that GA<sub>3</sub> might be involved in the developmental regulation of <italic>CiLFY</italic> during flowering process of precocious trifoliate orange. These results provided insights into the molecular regulation of <italic>CiLFY</italic> gene expression, which would be helpful for studying citrus flowering.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Li, C J" uniqKey="Li C">C-j Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mohandas, T" uniqKey="Mohandas T">T Mohandas</name></author><author><name sortKey="Sparkes, Rs" uniqKey="Sparkes R">RS Sparkes</name></author><author><name sortKey="Shapiro, Lj" uniqKey="Shapiro L">LJ Shapiro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miura, A" uniqKey="Miura A">A Miura</name></author><author><name sortKey="Yonebayashi, S" uniqKey="Yonebayashi S">S Yonebayashi</name></author><author><name sortKey="Watanabe, K" uniqKey="Watanabe K">K Watanabe</name></author><author><name sortKey="Toyama, T" uniqKey="Toyama T">T Toyama</name></author><author><name sortKey="Shimada, H" uniqKey="Shimada H">H Shimada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bird, A" uniqKey="Bird A">A Bird</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, E" uniqKey="Li E">E Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shibukawa, T" uniqKey="Shibukawa T">T Shibukawa</name></author><author><name sortKey="Yazawa, K" uniqKey="Yazawa K">K Yazawa</name></author><author><name sortKey="Kikuchi, A" uniqKey="Kikuchi A">A Kikuchi</name></author><author><name sortKey="Kamada, H" uniqKey="Kamada H">H Kamada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hashida, Sn" uniqKey="Hashida S">SN Hashida</name></author><author><name sortKey="Uchiyama, T" uniqKey="Uchiyama T">T Uchiyama</name></author><author><name sortKey="Martin, C" uniqKey="Martin C">C Martin</name></author><author><name sortKey="Kishima, Y" uniqKey="Kishima Y">Y Kishima</name></author><author><name sortKey="Sano, Y" uniqKey="Sano Y">Y Sano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Herman, Jg" uniqKey="Herman J">JG Herman</name></author><author><name sortKey="Baylin, Sb" uniqKey="Baylin S">SB Baylin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krawczyk, J" uniqKey="Krawczyk J">J Krawczyk</name></author><author><name sortKey="Keane, N" uniqKey="Keane N">N Keane</name></author><author><name sortKey="Freeman, Cl" uniqKey="Freeman C">CL Freeman</name></author><author><name sortKey="Swords, R" uniqKey="Swords R">R Swords</name></author><author><name sortKey="O Wyer, M" uniqKey="O Wyer M">M O′Dwyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cao, X" uniqKey="Cao X">X Cao</name></author><author><name sortKey="Jacobsen, Se" uniqKey="Jacobsen S">SE Jacobsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vanyushin, Bf" uniqKey="Vanyushin B">BF Vanyushin</name></author><author><name sortKey="Bakeeva, Le" uniqKey="Bakeeva L">LE Bakeeva</name></author><author><name sortKey="Zamyatnina, Va" uniqKey="Zamyatnina V">VA Zamyatnina</name></author><author><name sortKey="Aleksandrushkina, Ni" uniqKey="Aleksandrushkina N">NI Aleksandrushkina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Manero, G" uniqKey="Garcia Manero G">G Garcia-Manero</name></author><author><name sortKey="Daniel, J" uniqKey="Daniel J">J Daniel</name></author><author><name sortKey="Smith, Tl" uniqKey="Smith T">TL Smith</name></author><author><name sortKey="Kornblau, Sm" uniqKey="Kornblau S">SM Kornblau</name></author><author><name sortKey="Lee, M S" uniqKey="Lee M">M-S Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marfil, Cf" uniqKey="Marfil C">CF Marfil</name></author><author><name sortKey="Asurmendi, S" uniqKey="Asurmendi S">S Asurmendi</name></author><author><name sortKey="Masuelli, Rw" uniqKey="Masuelli R">RW Masuelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kondo, H" uniqKey="Kondo H">H Kondo</name></author><author><name sortKey="Shiraya, T" uniqKey="Shiraya T">T Shiraya</name></author><author><name sortKey="Wada, Kc" uniqKey="Wada K">KC Wada</name></author><author><name sortKey="Takeno, K" uniqKey="Takeno K">K Takeno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gehring, M" uniqKey="Gehring M">M Gehring</name></author><author><name sortKey="Huh, Jh" uniqKey="Huh J">JH Huh</name></author><author><name sortKey="Hsieh, Tf" uniqKey="Hsieh T">TF Hsieh</name></author><author><name sortKey="Penterman, J" uniqKey="Penterman J">J Penterman</name></author><author><name sortKey="Choi, Y" uniqKey="Choi Y">Y Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jullien, Pe" uniqKey="Jullien P">PE Jullien</name></author><author><name sortKey="Kinoshita, T" uniqKey="Kinoshita T">T Kinoshita</name></author><author><name sortKey="Ohad, N" uniqKey="Ohad N">N Ohad</name></author><author><name sortKey="Berger, F" uniqKey="Berger F">F Berger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kinoshita, T" uniqKey="Kinoshita T">T Kinoshita</name></author><author><name sortKey="Miura, A" uniqKey="Miura A">A Miura</name></author><author><name sortKey="Choi, Y" uniqKey="Choi Y">Y Choi</name></author><author><name sortKey="Kinoshita, Y" uniqKey="Kinoshita Y">Y Kinoshita</name></author><author><name sortKey="Cao, X" uniqKey="Cao X">X Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kakutani, T" uniqKey="Kakutani T">T Kakutani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Soppe, Wjj" uniqKey="Soppe W">WJJ Soppe</name></author><author><name sortKey="Jacobsen, Se" uniqKey="Jacobsen S">SE Jacobsen</name></author><author><name sortKey="Alonso Blanco, C" uniqKey="Alonso Blanco C">C Alonso-Blanco</name></author><author><name sortKey="Jackson, Jp" uniqKey="Jackson J">JP Jackson</name></author><author><name sortKey="Kakutani, T" uniqKey="Kakutani T">T Kakutani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Preuss, D" uniqKey="Preuss D">D Preuss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lippman, Z" uniqKey="Lippman Z">Z Lippman</name></author><author><name sortKey="Gendrel, Av" uniqKey="Gendrel A">AV Gendrel</name></author><author><name sortKey="Black, M" uniqKey="Black M">M Black</name></author><author><name sortKey="Vaughn, Mw" uniqKey="Vaughn M">MW Vaughn</name></author><author><name sortKey="Dedhia, N" uniqKey="Dedhia N">N Dedhia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Sw" uniqKey="Chan S">SW Chan</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Bernatavichute, Yv" uniqKey="Bernatavichute Y">YV Bernatavichute</name></author><author><name sortKey="Jacobsen, Se" uniqKey="Jacobsen S">SE Jacobsen</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Burn, Je" uniqKey="Burn J">JE Burn</name></author><author><name sortKey="Bagnall, Dj" uniqKey="Bagnall D">DJ Bagnall</name></author><author><name sortKey="Metzger, Jd" uniqKey="Metzger J">JD Metzger</name></author><author><name sortKey="Dennis, Es" uniqKey="Dennis E">ES Dennis</name></author><author><name sortKey="Peacock, Wj" uniqKey="Peacock W">WJ Peacock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pe A, L" uniqKey="Pe A L">L Peña</name></author><author><name sortKey="Martin Trillo, M" uniqKey="Martin Trillo M">M Martín-Trillo</name></author><author><name sortKey="Juarez, J" uniqKey="Juarez J">J Juárez</name></author><author><name sortKey="Pina, Ja" uniqKey="Pina J">JA Pina</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, Fc" uniqKey="Tan F">FC Tan</name></author><author><name sortKey="Swain, Sm" uniqKey="Swain S">SM Swain</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pillitteri, Lj" uniqKey="Pillitteri L">LJ Pillitteri</name></author><author><name sortKey="Lovatt, Cj" uniqKey="Lovatt C">CJ Lovatt</name></author><author><name sortKey="Walling, Ll" uniqKey="Walling L">LL Walling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="El Otmani, M" uniqKey="El Otmani M">M El-Otmani</name></author><author><name sortKey="Coggins, Cw" uniqKey="Coggins C">CW Coggins</name></author><author><name sortKey="Agusti, M" uniqKey="Agusti M">M Agusti</name></author><author><name sortKey="Lovatt, Cj" uniqKey="Lovatt C">CJ Lovatt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Jz" uniqKey="Zhang J">JZ Zhang</name></author><author><name sortKey="Li, Zm" uniqKey="Li Z">ZM Li</name></author><author><name sortKey="Mei, L" uniqKey="Mei L">L Mei</name></author><author><name sortKey="Yao, Jl" uniqKey="Yao J">JL Yao</name></author><author><name sortKey="Hu, Cg" uniqKey="Hu C">CG Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Jz" uniqKey="Zhang J">JZ Zhang</name></author><author><name sortKey="Li, Zm" uniqKey="Li Z">ZM Li</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Mei, L" uniqKey="Mei L">L Mei</name></author><author><name sortKey="Yao, Jl" uniqKey="Yao J">JL Yao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kinoshita, Y" uniqKey="Kinoshita Y">Y Kinoshita</name></author><author><name sortKey="Saze, H" uniqKey="Saze H">H Saze</name></author><author><name sortKey="Kinoshita, T" uniqKey="Kinoshita T">T Kinoshita</name></author><author><name sortKey="Miura, A" uniqKey="Miura A">A Miura</name></author><author><name sortKey="Soppe, Wjj" uniqKey="Soppe W">WJJ Soppe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pillitteri, Lj" uniqKey="Pillitteri L">LJ Pillitteri</name></author><author><name sortKey="Lovatt, Cj" uniqKey="Lovatt C">CJ Lovatt</name></author><author><name sortKey="Walling, Ll" uniqKey="Walling L">LL Walling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Jz" uniqKey="Zhang J">JZ Zhang</name></author><author><name sortKey="Li, Zm" uniqKey="Li Z">ZM Li</name></author><author><name sortKey="Yao, Jl" uniqKey="Yao J">JL Yao</name></author><author><name sortKey="Hu, Cg" uniqKey="Hu C">CG Hu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author><author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name></author><author><name sortKey="Yu, P" uniqKey="Yu P">P Yu</name></author><author><name sortKey="Cohen, Jd" uniqKey="Cohen J">JD Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weigel, D" uniqKey="Weigel D">D Weigel</name></author><author><name sortKey="Nilsson, O" uniqKey="Nilsson O">O Nilsson</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Liang, S" uniqKey="Liang S">S Liang</name></author><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Wan, T" uniqKey="Wan T">T Wan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Castilho, A" uniqKey="Castilho A">A Castilho</name></author><author><name sortKey="Neves, N" uniqKey="Neves N">N Neves</name></author><author><name sortKey="Rufini Castiglione, M" uniqKey="Rufini Castiglione M">M Rufini-Castiglione</name></author><author><name sortKey="Viegas, W" uniqKey="Viegas W">W Viegas</name></author><author><name sortKey="Heslop Harrison, Js" uniqKey="Heslop Harrison J">JS Heslop-Harrison</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brown, Jcl" uniqKey="Brown J">JCL Brown</name></author><author><name sortKey="De Decker, Mm" uniqKey="De Decker M">MM De Decker</name></author><author><name sortKey="Fieldes, Ma" uniqKey="Fieldes M">MA Fieldes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Iwase, Y" uniqKey="Iwase Y">Y Iwase</name></author><author><name sortKey="Shiraya, T" uniqKey="Shiraya T">T Shiraya</name></author><author><name sortKey="Takeno, K" uniqKey="Takeno K">K Takeno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kondo, H" uniqKey="Kondo H">H Kondo</name></author><author><name sortKey="Miura, T" uniqKey="Miura T">T Miura</name></author><author><name sortKey="Wada, Kc" uniqKey="Wada K">KC Wada</name></author><author><name sortKey="Takeno, K" uniqKey="Takeno K">K Takeno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, Yj" uniqKey="Cheng Y">YJ Cheng</name></author><author><name sortKey="Guo, Ww" uniqKey="Guo W">WW Guo</name></author><author><name sortKey="Yi, Hl" uniqKey="Yi H">HL Yi</name></author><author><name sortKey="Pang, Xm" uniqKey="Pang X">XM Pang</name></author><author><name sortKey="Deng, X" uniqKey="Deng X">X Deng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jacobsen, Se" uniqKey="Jacobsen S">SE Jacobsen</name></author><author><name sortKey="Sakai, H" uniqKey="Sakai H">H Sakai</name></author><author><name sortKey="Finnegan, Ej" uniqKey="Finnegan E">EJ Finnegan</name></author><author><name sortKey="Cao, X" uniqKey="Cao X">X Cao</name></author><author><name sortKey="Meyerowitz, Em" uniqKey="Meyerowitz E">EM Meyerowitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clough, Sj" uniqKey="Clough S">SJ Clough</name></author><author><name sortKey="Bent, Af" uniqKey="Bent A">AF Bent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murashige, T" uniqKey="Murashige T">T Murashige</name></author><author><name sortKey="Skoog, F" uniqKey="Skoog F">F Skoog</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Blazquez, Ma" uniqKey="Blazquez M">MA Blázquez</name></author><author><name sortKey="Green, R" uniqKey="Green R">R Green</name></author><author><name sortKey="Nilsson, O" uniqKey="Nilsson O">O Nilsson</name></author><author><name sortKey="Sussman, Mr" uniqKey="Sussman M">MR Sussman</name></author><author><name sortKey="Weigel, D" uniqKey="Weigel D">D Weigel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jefferson, Ra" uniqKey="Jefferson R">RA Jefferson</name></author><author><name sortKey="Kavanagh, Ta" uniqKey="Kavanagh T">TA Kavanagh</name></author><author><name sortKey="Bevan, Mw" uniqKey="Bevan M">MW Bevan</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Christman, Jk" uniqKey="Christman J">JK Christman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Finnegan, Ej" uniqKey="Finnegan E">EJ Finnegan</name></author><author><name sortKey="Peacock, Wj" uniqKey="Peacock W">WJ Peacock</name></author><author><name sortKey="Dennis, Es" uniqKey="Dennis E">ES Dennis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kondo, H" uniqKey="Kondo H">H Kondo</name></author><author><name sortKey="Ozaki, H" uniqKey="Ozaki H">H Ozaki</name></author><author><name sortKey="Itoh, K" uniqKey="Itoh K">K Itoh</name></author><author><name sortKey="Kato, A" uniqKey="Kato A">A Kato</name></author><author><name sortKey="Takeno, K" uniqKey="Takeno K">K Takeno</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michaels, Sd" uniqKey="Michaels S">SD Michaels</name></author><author><name sortKey="Amasino, Rm" uniqKey="Amasino R">RM Amasino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michaels, Sd" uniqKey="Michaels S">SD Michaels</name></author><author><name sortKey="Amasino, Rm" uniqKey="Amasino R">RM Amasino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carmona, Mj" uniqKey="Carmona M">MJ Carmona</name></author><author><name sortKey="Cubas, P" uniqKey="Cubas P">P Cubas</name></author><author><name sortKey="Martinez Zapater, Jm" uniqKey="Martinez Zapater J">JM Martinez-Zapater</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hsu, Cy" uniqKey="Hsu C">CY Hsu</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Luthe, Ds" uniqKey="Luthe D">DS Luthe</name></author><author><name sortKey="Yuceer, C" uniqKey="Yuceer C">C Yuceer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nishikawa, F" uniqKey="Nishikawa F">F Nishikawa</name></author><author><name sortKey="Endo, T" uniqKey="Endo T">T Endo</name></author><author><name sortKey="Shimada, T" uniqKey="Shimada T">T Shimada</name></author><author><name sortKey="Fujii, H" uniqKey="Fujii H">H Fujii</name></author><author><name sortKey="Shimizu, T" uniqKey="Shimizu T">T Shimizu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nishikawa, F" uniqKey="Nishikawa F">F Nishikawa</name></author><author><name sortKey="Endo, T" uniqKey="Endo T">T Endo</name></author><author><name sortKey="Shimada, T" uniqKey="Shimada T">T Shimada</name></author><author><name sortKey="Fujii, H" uniqKey="Fujii H">H Fujii</name></author><author><name sortKey="Shimizu, T" uniqKey="Shimizu T">T Shimizu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michaels, Sd" uniqKey="Michaels S">SD Michaels</name></author><author><name sortKey="Amasino, Rm" uniqKey="Amasino R">RM Amasino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kakutani, T" uniqKey="Kakutani T">T Kakutani</name></author><author><name sortKey="Munakata, K" uniqKey="Munakata K">K Munakata</name></author><author><name sortKey="Richards, Ej" uniqKey="Richards E">EJ Richards</name></author><author><name sortKey="Hirochika, H" uniqKey="Hirochika H">H Hirochika</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ronemus, Mj" uniqKey="Ronemus M">MJ Ronemus</name></author><author><name sortKey="Galbiati, M" uniqKey="Galbiati M">M Galbiati</name></author><author><name sortKey="Ticknor, C" uniqKey="Ticknor C">C Ticknor</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Dellaporta, Sl" uniqKey="Dellaporta S">SL Dellaporta</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Giannino, D" uniqKey="Giannino D">D Giannino</name></author><author><name sortKey="Mele, G" uniqKey="Mele G">G Mele</name></author><author><name sortKey="Cozza, R" uniqKey="Cozza R">R Cozza</name></author><author><name sortKey="Bruno, L" uniqKey="Bruno L">L Bruno</name></author><author><name sortKey="Testone, G" uniqKey="Testone G">G Testone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melzer, S" uniqKey="Melzer S">S Melzer</name></author><author><name sortKey="Lens, F" uniqKey="Lens F">F Lens</name></author><author><name sortKey="Gennen, J" uniqKey="Gennen J">J Gennen</name></author><author><name sortKey="Vanneste, S" uniqKey="Vanneste S">S Vanneste</name></author><author><name sortKey="Rohde, A" uniqKey="Rohde A">A Rohde</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fraga, Mf" uniqKey="Fraga M">MF Fraga</name></author><author><name sortKey="Uriol, E" uniqKey="Uriol E">E Uriol</name></author><author><name sortKey="Diego, Lb" uniqKey="Diego L">LB Diego</name></author><author><name sortKey="Berdasco, M" uniqKey="Berdasco M">M Berdasco</name></author><author><name sortKey="Esteller, M" uniqKey="Esteller M">M Esteller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Genger, Rk" uniqKey="Genger R">RK Genger</name></author><author><name sortKey="Peacock, Jw" uniqKey="Peacock J">JW Peacock</name></author><author><name sortKey="Dennis, Es" uniqKey="Dennis E">ES Dennis</name></author><author><name sortKey="Finnegan, Je" uniqKey="Finnegan J">JE Finnegan</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24523915</article-id><article-id pub-id-type="pmc">3921215</article-id><article-id pub-id-type="publisher-id">PONE-D-13-52764</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0088558</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Agriculture</subject><subj-group><subject>Forestry</subject></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Developmental Biology</subject><subj-group><subject>Cell Differentiation</subject><subject>Organism Development</subject><subject>Plant Growth and Development</subject></subj-group></subj-group><subj-group><subject>Genetics</subject><subj-group><subject>Gene Expression</subject><subj-group><subject>DNA modification</subject><subject>DNA transcription</subject><subject>Chromatin</subject></subj-group></subj-group><subj-group><subject>Gene Function</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Possible Involvement of Locus-Specific Methylation on Expression Regulation of <italic>LEAFY</italic> Homologous Gene (<italic>CiLFY</italic>) during Precocious Trifoliate Orange Phase Change Process</article-title><alt-title alt-title-type="running-head">Methylation on Expression Regulation of <italic>CiLFY</italic></alt-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Zhang</surname><given-names>Jin-Zhi</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Mei</surname><given-names>Li</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="author-notes" rid="fn1"><sup>¤</sup></xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Rong</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Khan</surname><given-names>Muhammad Rehman Gul</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Chun-Gen</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, Hubei Province, China</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Sun</surname><given-names>Meng-xiang</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Wuhan University, China</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>chungen@mail.hzau.edu.cn</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: JZZ CGH. Performed the experiments: RL LM. Analyzed the data: RL LM JZZ MRGK. Wrote the paper: JZZ.</p></fn><fn id="fn1" fn-type="current-aff"><label>¤</label><p>Current address: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, LinAn, Zhejiang Province, China</p></fn></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>11</day><month>2</month><year>2014</year></pub-date><volume>9</volume><issue>2</issue><elocation-id>e88558</elocation-id><history><date date-type="received"><day>16</day><month>12</month><year>2013</year></date><date date-type="accepted"><day>7</day><month>1</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>Zhang et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><p>DNA methylation plays an essential role in regulating plant development. Here, we described an early flowering trifoliate orange (precocious trifoliate orange, <italic>Poncirus trifoliata</italic> L. Raf) was treated with 5-azacytidine and displayed a number of phenotypic and developmental abnormalities. These observations suggested that DNA methylation might play an important role in regulating many developmental pathways including early flowering trait, and then the expression level of five key or integrated citrus flowering genes were analyzed. Our results showed that <italic>FLOWERING LOCUS T</italic> (<italic>CiFT</italic>) relative expression level was increased with the increasing concentrations of 5-AzaC. However, <italic>LEAFY</italic> (<italic>CiLFY</italic>), <italic>APETELA1</italic> (<italic>CiAP1</italic>), <italic>TERMINAL FLOWER1</italic> (<italic>CiTFL1</italic>), and <italic>FLOWERING LOCUS C</italic> (<italic>CiFLC</italic>) showed highest relative expression levels at 250 µΜ treatment, while decreased sharply at higher concentrations. In order to further confirm DNA methylation affects the expression of these genes, their full-length sequences were isolated by genome-walker method, and then was analyzed by using bioinformatics tools. However, only one locus-specific methylation site was observed in <italic>CiLFY</italic> sequence. Therefore, DNA methylation level of the <italic>CiLFY</italic> was investigated both at juvenile and adult stages of precocious trifoliate orange by bisulfate sequencing PCR; it has been shown that the level of DNA methylation was altered during phase change. In addition, spatial and temporal expression patterns of <italic>CiLFY</italic> promoter and a series of 5′ deletions were investigated by driving the expression of a β-glucuronidase reporter gene in <italic>Arabidopsis</italic>. Exogenous GA<sub>3</sub> treatment on transgenic <italic>Arabidopsis</italic> revealed that GA<sub>3</sub> might be involved in the developmental regulation of <italic>CiLFY</italic> during flowering process of precocious trifoliate orange. These results provided insights into the molecular regulation of <italic>CiLFY</italic> gene expression, which would be helpful for studying citrus flowering.</p></abstract><funding-group><funding-statement>This research was supported financially by the Fundamental Research Funds for the National Natural Science Foundation of China (grant numbers 31130046, 31071777, and 31101528). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="10"></page-count></counts></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Hu, Chun Gen" sort="Hu, Chun Gen" uniqKey="Hu C" first="Chun-Gen" last="Hu">Chun-Gen Hu</name><name sortKey="Khan, Muhammad Rehman Gul" sort="Khan, Muhammad Rehman Gul" uniqKey="Khan M" first="Muhammad Rehman Gul" last="Khan">Muhammad Rehman Gul Khan</name><name sortKey="Liu, Rong" sort="Liu, Rong" uniqKey="Liu R" first="Rong" last="Liu">Rong Liu</name><name sortKey="Mei, Li" sort="Mei, Li" uniqKey="Mei L" first="Li" last="Mei">Li Mei</name><name sortKey="Zhang, Jin Zhi" sort="Zhang, Jin Zhi" uniqKey="Zhang J" first="Jin-Zhi" last="Zhang">Jin-Zhi Zhang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001597 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001597 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= PMC:3921215
|texte= Possible Involvement of Locus-Specific Methylation on Expression Regulation of LEAFY Homologous Gene (CiLFY) during Precocious Trifoliate Orange Phase Change Process
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:24523915" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |