Green fluorescent protein: an in vivo reporter of plant gene expression.
Identifieur interne : 001472 ( Ncbi/Merge ); précédent : 001471; suivant : 001473Green fluorescent protein: an in vivo reporter of plant gene expression.
Auteurs : R P Niedz [États-Unis] ; M R Sussman ; J S SatterleeSource :
- Plant cell reports [ 0721-7714 ] ; 1995.
Abstract
Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.
DOI: 10.1007/BF00234043
PubMed: 24185445
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000F42
- to stream PubMed, to step Curation: 000F42
- to stream PubMed, to step Checkpoint: 000F42
Links to Exploration step
pubmed:24185445Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Green fluorescent protein: an in vivo reporter of plant gene expression.</title>
<author><name sortKey="Niedz, R P" sort="Niedz, R P" uniqKey="Niedz R" first="R P" last="Niedz">R P Niedz</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, 2120 Camden Road, 32803, Orlando, FL, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, 2120 Camden Road, 32803, Orlando, FL</wicri:regionArea>
<placeName><region type="state">Floride</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sussman, M R" sort="Sussman, M R" uniqKey="Sussman M" first="M R" last="Sussman">M R Sussman</name>
</author>
<author><name sortKey="Satterlee, J S" sort="Satterlee, J S" uniqKey="Satterlee J" first="J S" last="Satterlee">J S Satterlee</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1995">1995</date>
<idno type="RBID">pubmed:24185445</idno>
<idno type="pmid">24185445</idno>
<idno type="doi">10.1007/BF00234043</idno>
<idno type="wicri:Area/PubMed/Corpus">000F42</idno>
<idno type="wicri:Area/PubMed/Curation">000F42</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000F42</idno>
<idno type="wicri:Area/Ncbi/Merge">001472</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Green fluorescent protein: an in vivo reporter of plant gene expression.</title>
<author><name sortKey="Niedz, R P" sort="Niedz, R P" uniqKey="Niedz R" first="R P" last="Niedz">R P Niedz</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, 2120 Camden Road, 32803, Orlando, FL, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, 2120 Camden Road, 32803, Orlando, FL</wicri:regionArea>
<placeName><region type="state">Floride</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sussman, M R" sort="Sussman, M R" uniqKey="Sussman M" first="M R" last="Sussman">M R Sussman</name>
</author>
<author><name sortKey="Satterlee, J S" sort="Satterlee, J S" uniqKey="Satterlee J" first="J S" last="Satterlee">J S Satterlee</name>
</author>
</analytic>
<series><title level="j">Plant cell reports</title>
<idno type="ISSN">0721-7714</idno>
<imprint><date when="1995" type="published">1995</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24185445</PMID>
<DateCreated><Year>2013</Year>
<Month>11</Month>
<Day>4</Day>
</DateCreated>
<DateCompleted><Year>2013</Year>
<Month>11</Month>
<Day>05</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>4</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0721-7714</ISSN>
<JournalIssue CitedMedium="Print"><Volume>14</Volume>
<Issue>7</Issue>
<PubDate><Year>1995</Year>
<Month>Apr</Month>
</PubDate>
</JournalIssue>
<Title>Plant cell reports</Title>
<ISOAbbreviation>Plant Cell Rep.</ISOAbbreviation>
</Journal>
<ArticleTitle>Green fluorescent protein: an in vivo reporter of plant gene expression.</ArticleTitle>
<Pagination><MedlinePgn>403-6</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/BF00234043</ELocationID>
<Abstract><AbstractText>Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Niedz</LastName>
<ForeName>R P</ForeName>
<Initials>RP</Initials>
<AffiliationInfo><Affiliation>U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, 2120 Camden Road, 32803, Orlando, FL, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sussman</LastName>
<ForeName>M R</ForeName>
<Initials>MR</Initials>
</Author>
<Author ValidYN="Y"><LastName>Satterlee</LastName>
<ForeName>J S</ForeName>
<Initials>JS</Initials>
</Author>
</AuthorList>
<Language>ENG</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Plant Cell Rep</MedlineTA>
<NlmUniqueID>9880970</NlmUniqueID>
<ISSNLinking>0721-7714</ISSNLinking>
</MedlineJournalInfo>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>1994</Year>
<Month>9</Month>
<Day>7</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>1994</Year>
<Month>10</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2013</Year>
<Month>11</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>1995</Year>
<Month>4</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1995</Year>
<Month>4</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">24185445</ArticleId>
<ArticleId IdType="doi">10.1007/BF00234043</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Floride</li>
</region>
</list>
<tree><noCountry><name sortKey="Satterlee, J S" sort="Satterlee, J S" uniqKey="Satterlee J" first="J S" last="Satterlee">J S Satterlee</name>
<name sortKey="Sussman, M R" sort="Sussman, M R" uniqKey="Sussman M" first="M R" last="Sussman">M R Sussman</name>
</noCountry>
<country name="États-Unis"><region name="Floride"><name sortKey="Niedz, R P" sort="Niedz, R P" uniqKey="Niedz R" first="R P" last="Niedz">R P Niedz</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001472 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001472 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Ncbi |étape= Merge |type= RBID |clé= pubmed:24185445 |texte= Green fluorescent protein: an in vivo reporter of plant gene expression. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:24185445" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
This area was generated with Dilib version V0.6.25. |