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Green fluorescent protein: an in vivo reporter of plant gene expression.

Identifieur interne : 000F42 ( PubMed/Curation ); précédent : 000F41; suivant : 000F43

Green fluorescent protein: an in vivo reporter of plant gene expression.

Auteurs : R P Niedz [États-Unis] ; M R Sussman ; J S Satterlee

Source :

Plant cell reports [ 0721-7714 ] ; 1995.

RBID : pubmed:24185445

Abstract

Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.

DOI: 10.1007/BF00234043
PubMed: 24185445

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pubmed:24185445

Le document en format XML

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<wicri:regionArea>U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, 2120 Camden Road, 32803, Orlando, FL</wicri:regionArea>
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<front><div type="abstract" xml:lang="en">Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.</div>
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<Abstract><AbstractText>Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.</AbstractText>
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Green fluorescent protein: an in vivo reporter of plant gene expression.

Green fluorescent protein: an in vivo reporter of plant gene expression.

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki