Untargeted metabolite analysis of healthy and Huanglongbing-infected orange leaves by CE-DAD.
Identifieur interne : 000968 ( Ncbi/Curation ); précédent : 000967; suivant : 000969Untargeted metabolite analysis of healthy and Huanglongbing-infected orange leaves by CE-DAD.
Auteurs : Juan Manuel Cevallos-Cevallos [États-Unis] ; Russell Rouseff ; José I. Reyes-De-CorcueraSource :
- Electrophoresis [ 1522-2683 ] ; 2009.
English descriptors
- KwdEn :
- Citrus sinensis (metabolism), Citrus sinensis (microbiology), Electrophoresis, Capillary (methods), Flavanones (analysis), Flavanones (metabolism), Hesperidin (analysis), Hesperidin (metabolism), Metabolomics (methods), Plant Diseases (microbiology), Plant Extracts (analysis), Plant Extracts (metabolism), Plant Leaves (metabolism), Plant Leaves (microbiology), Quercetin (analysis), Quercetin (metabolism).
- MESH :
- chemical , analysis : Flavanones, Hesperidin, Plant Extracts, Quercetin.
- metabolism : Citrus sinensis, Flavanones, Hesperidin, Plant Extracts, Plant Leaves, Quercetin.
- methods : Electrophoresis, Capillary, Metabolomics.
- microbiology : Citrus sinensis, Plant Diseases, Plant Leaves.
Abstract
Huanglongbing (HLB) is considered the most destructive bacterial citrus disease worldwide. Early detection of HLB is crucial for minimizing its spread. CE was used for the discovery of potential biomarkers for HLB. Optimization of extraction and separation allowed resolving 24 compounds of which 6 were present in significantly higher (p<0.05) concentrations in HLB-infected samples collected monthly for 6 months during the 2007-2008 season. Three of these compounds were identified by mobility and UV spectra as hesperidin, naringenin, and quercetin with mean increase in concentration of 154, 555, and 467%, respectively, above that in healthy leaves. Results support the potential of CE-DAD for untargeted plant metabolomic analysis. CZE, NACE, and MEEKC were compared for metabolic differentiation of healthy and HLB-infected citrus leaves. CZE in a semi-aqueous BGE solution consisting of 8.5 mM of sodium borate (pH 9.3), 15% ACN, and 9% 1-butanol yielded the best peak separation with detection at 190 nm.
DOI: 10.1002/elps.200800594
PubMed: 19283697
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pubmed:19283697Le document en format XML
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Reyes-De-Corcuera</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19283697</idno><idno type="pmid">19283697</idno><idno type="doi">10.1002/elps.200800594</idno><idno type="wicri:Area/PubMed/Corpus">000963</idno><idno type="wicri:Area/PubMed/Curation">000963</idno><idno type="wicri:Area/PubMed/Checkpoint">000963</idno><idno type="wicri:Area/Ncbi/Merge">000968</idno><idno type="wicri:Area/Ncbi/Curation">000968</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Untargeted metabolite analysis of healthy and Huanglongbing-infected orange leaves by CE-DAD.</title><author><name sortKey="Cevallos Cevallos, Juan Manuel" sort="Cevallos Cevallos, Juan Manuel" uniqKey="Cevallos Cevallos J" first="Juan Manuel" last="Cevallos-Cevallos">Juan Manuel Cevallos-Cevallos</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research and Education Center, IFAS, University of Florida, FL, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research and Education Center, IFAS, University of Florida, FL</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Rouseff, Russell" sort="Rouseff, Russell" uniqKey="Rouseff R" first="Russell" last="Rouseff">Russell Rouseff</name></author><author><name sortKey="Reyes De Corcuera, Jose I" sort="Reyes De Corcuera, Jose I" uniqKey="Reyes De Corcuera J" first="José I" last="Reyes-De-Corcuera">José I. Reyes-De-Corcuera</name></author></analytic><series><title level="j">Electrophoresis</title><idno type="eISSN">1522-2683</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis (metabolism)</term><term>Citrus sinensis (microbiology)</term><term>Electrophoresis, Capillary (methods)</term><term>Flavanones (analysis)</term><term>Flavanones (metabolism)</term><term>Hesperidin (analysis)</term><term>Hesperidin (metabolism)</term><term>Metabolomics (methods)</term><term>Plant Diseases (microbiology)</term><term>Plant Extracts (analysis)</term><term>Plant Extracts (metabolism)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Quercetin (analysis)</term><term>Quercetin (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Flavanones</term><term>Hesperidin</term><term>Plant Extracts</term><term>Quercetin</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term><term>Flavanones</term><term>Hesperidin</term><term>Plant Extracts</term><term>Plant Leaves</term><term>Quercetin</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Capillary</term><term>Metabolomics</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Huanglongbing (HLB) is considered the most destructive bacterial citrus disease worldwide. Early detection of HLB is crucial for minimizing its spread. CE was used for the discovery of potential biomarkers for HLB. Optimization of extraction and separation allowed resolving 24 compounds of which 6 were present in significantly higher (p<0.05) concentrations in HLB-infected samples collected monthly for 6 months during the 2007-2008 season. Three of these compounds were identified by mobility and UV spectra as hesperidin, naringenin, and quercetin with mean increase in concentration of 154, 555, and 467%, respectively, above that in healthy leaves. Results support the potential of CE-DAD for untargeted plant metabolomic analysis. CZE, NACE, and MEEKC were compared for metabolic differentiation of healthy and HLB-infected citrus leaves. CZE in a semi-aqueous BGE solution consisting of 8.5 mM of sodium borate (pH 9.3), 15% ACN, and 9% 1-butanol yielded the best peak separation with detection at 190 nm.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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