Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins
Identifieur interne : 000891 ( Ncbi/Curation ); précédent : 000890; suivant : 000892Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins
Auteurs : Simon Conn ; Chris Curtin ; Annie Bézier [France] ; Chris Franco ; Wei ZhangSource :
- Journal of Experimental Botany [ 0022-0957 ] ; 2008.
Abstract
The ligandin activity of specific glutathione
Url:
DOI: 10.1093/jxb/ern217
PubMed: 18836188
PubMed Central: 2561157
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Simon Conn<affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff2">School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff2">School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff3">Australian Wine Research Institute, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff3">Australian Wine Research Institute, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Purification, molecular cloning, and characterization of glutathione <italic>S</italic>-transferases (GSTs) from pigmented <italic>Vitis vinifera</italic> L. cell suspension cultures as putative anthocyanin transport proteins</title><author><name sortKey="Conn, Simon" sort="Conn, Simon" uniqKey="Conn S" first="Simon" last="Conn">Simon Conn</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff2">School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation></author><author><name sortKey="Curtin, Chris" sort="Curtin, Chris" uniqKey="Curtin C" first="Chris" last="Curtin">Chris Curtin</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff3">Australian Wine Research Institute, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation></author><author><name sortKey="Bezier, Annie" sort="Bezier, Annie" uniqKey="Bezier A" first="Annie" last="Bézier">Annie Bézier</name><affiliation wicri:level="4"><nlm:aff id="aff4">Laboratoire de Biologie et Physiologie Végétales, Equipe de Biochimie et Biologie Moléculaire des Plantes, Université de Reims Champagne-Ardenne, 51687 Reims cedex 2, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie et Physiologie Végétales, Equipe de Biochimie et Biologie Moléculaire des Plantes, Université de Reims Champagne-Ardenne, 51687 Reims cedex 2</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Champagne-Ardenne</region><settlement type="city">Reims</settlement></placeName><orgName type="university">Université de Reims Champagne-Ardenne</orgName></affiliation></author><author><name sortKey="Franco, Chris" sort="Franco, Chris" uniqKey="Franco C" first="Chris" last="Franco">Chris Franco</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18836188</idno><idno type="pmc">2561157</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2561157</idno><idno type="RBID">PMC:2561157</idno><idno type="doi">10.1093/jxb/ern217</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000A77</idno><idno type="wicri:Area/Pmc/Curation">000A76</idno><idno type="wicri:Area/Pmc/Checkpoint">001062</idno><idno type="wicri:Area/Ncbi/Merge">000891</idno><idno type="wicri:Area/Ncbi/Curation">000891</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Purification, molecular cloning, and characterization of glutathione <italic>S</italic>-transferases (GSTs) from pigmented <italic>Vitis vinifera</italic> L. cell suspension cultures as putative anthocyanin transport proteins</title><author><name sortKey="Conn, Simon" sort="Conn, Simon" uniqKey="Conn S" first="Simon" last="Conn">Simon Conn</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff2">School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation></author><author><name sortKey="Curtin, Chris" sort="Curtin, Chris" uniqKey="Curtin C" first="Chris" last="Curtin">Chris Curtin</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation><affiliation><nlm:aff id="aff3">Australian Wine Research Institute, Waite Campus, Urrbrae, Australia, 5064</nlm:aff><wicri:noCountry code="subfield">5064</wicri:noCountry></affiliation></author><author><name sortKey="Bezier, Annie" sort="Bezier, Annie" uniqKey="Bezier A" first="Annie" last="Bézier">Annie Bézier</name><affiliation wicri:level="4"><nlm:aff id="aff4">Laboratoire de Biologie et Physiologie Végétales, Equipe de Biochimie et Biologie Moléculaire des Plantes, Université de Reims Champagne-Ardenne, 51687 Reims cedex 2, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie et Physiologie Végétales, Equipe de Biochimie et Biologie Moléculaire des Plantes, Université de Reims Champagne-Ardenne, 51687 Reims cedex 2</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Champagne-Ardenne</region><settlement type="city">Reims</settlement></placeName><orgName type="university">Université de Reims Champagne-Ardenne</orgName></affiliation></author><author><name sortKey="Franco, Chris" sort="Franco, Chris" uniqKey="Franco C" first="Chris" last="Franco">Chris Franco</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name><affiliation><nlm:aff id="aff1">Department of Medical Biotechnology, Flinders University, Adelaide, Australia, 5042</nlm:aff><wicri:noCountry code="subfield">5042</wicri:noCountry></affiliation></author></analytic><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The ligandin activity of specific glutathione <italic>S</italic>-transferases (GSTs) is necessary for the transport of anthocyanins from the cytosol to the plant vacuole. Five GSTs were purified from <italic>Vitis vinifera</italic> L. cv. Gamay Fréaux cell suspension cultures by glutathione affinity chromatography. These proteins underwent Edman sequencing and mass spectrometry fingerprinting, with the resultant fragments aligned with predicted GSTs within public databases. The corresponding coding sequences were cloned, with heterologous expression in <italic>Escherichia coli</italic> used to confirm GST activity. Transcriptional profiling of these candidate GST genes and key anthocyanin biosynthetic pathway genes (<italic>PAL</italic>, <italic>CHS</italic>, <italic>DFR</italic>, and <italic>UFGT</italic>) in cell suspensions and grape berries against anthocyanin accumulation demonstrated strong positive correlation with two sequences, <italic>VvGST1</italic> and <italic>VvGST4</italic>, respectively. The ability of VvGST1 and VvGST4 to transport anthocyanins was confirmed in the heterologous maize <italic>bronze-2</italic> complementation model, providing further evidence for their function as anthocyanin transport proteins in grape cells. Furthermore, the differential induction of VvGST1 and VvGST4 in suspension cells and grape berries suggests functional differences between these two proteins. Further investigation of these candidate ligandins may identify a mechanism for manipulating anthocyanin accumulation <italic>in planta</italic> and <italic>in vitro</italic> suspension cells.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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