The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri
Identifieur interne : 001F71 ( Ncbi/Checkpoint ); précédent : 001F70; suivant : 001F72The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri
Auteurs : Laís Moreira Granato ; Simone Cristina Picchi ; Maxuel De Oliveira Andrade [États-Unis] ; Marco Aurélio Takita ; Alessandra Alves De Souza ; Nian Wang [États-Unis] ; Marcos Antonio MachadoSource :
- BMC Microbiology [ 1471-2180 ] ; 2016.
Abstract
RNA helicases are enzymes that catalyze the separation of double-stranded RNA (dsRNA) using the free energy of ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects of RNA metabolism, including RNA synthesis, RNA folding, RNA-RNA interactions, RNA localization and RNA degradation. Several important bacterial DEAD/DEAH-box RNA helicases have been extensively studied. In this study, we characterize the ATP-dependent RNA helicase encoded by the
The
Our data show that
The online version of this article (doi:10.1186/s12866-016-0655-1) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12866-016-0655-1
PubMed: 27005008
PubMed Central: 4804567
Affiliations:
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<author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in <italic>Xanthomonas citri</italic>
subsp. <italic>citri</italic>
</title>
<author><name sortKey="Granato, Lais Moreira" sort="Granato, Lais Moreira" uniqKey="Granato L" first="Laís Moreira" last="Granato">Laís Moreira Granato</name>
<affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff>
<wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff2">Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</nlm:aff>
<wicri:noCountry code="subfield">SP 13083-970 Brazil</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Picchi, Simone Cristina" sort="Picchi, Simone Cristina" uniqKey="Picchi S" first="Simone Cristina" last="Picchi">Simone Cristina Picchi</name>
<affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff>
<wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Andrade, Maxuel De Oliveira" sort="Andrade, Maxuel De Oliveira" uniqKey="Andrade M" first="Maxuel De Oliveira" last="Andrade">Maxuel De Oliveira Andrade</name>
<affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Floride</region>
</placeName>
<wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
<affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff>
<wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
<affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff>
<wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name>
<affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Floride</region>
</placeName>
<wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
<affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff>
<wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Microbiology</title>
<idno type="eISSN">1471-2180</idno>
<imprint><date when="2016">2016</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>RNA helicases are enzymes that catalyze the separation of double-stranded RNA (dsRNA) using the free energy of ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects of RNA metabolism, including RNA synthesis, RNA folding, RNA-RNA interactions, RNA localization and RNA degradation. Several important bacterial DEAD/DEAH-box RNA helicases have been extensively studied. In this study, we characterize the ATP-dependent RNA helicase encoded by the <italic>hrpB</italic>
(XAC0293) gene using deletion and genetic complementation assays. We provide insights into the function of the <italic>hrpB</italic>
gene in <italic>Xanthomonas citri</italic>
subsp. <italic>citri</italic>
by investigating the roles of <italic>hrpB</italic>
in biofilm formation on abiotic surfaces and host leaves, cell motility, host virulence of the citrus canker bacterium and growth <italic>in planta</italic>
.</p>
</sec>
<sec><title>Results</title>
<p>The <italic>hrpB</italic>
gene is highly conserved in the sequenced strains of <italic>Xanthomonas</italic>
. Mutation of the <italic>hrpB</italic>
gene (<italic>∆hrpB</italic>
) resulted in a significant reduction in biofilms on abiotic surfaces and host leaves. <italic>∆hrpB</italic>
also exhibited increased cell dispersion on solid medium plates. <italic>∆hrpB</italic>
showed reduced adhesion on biotic and abiotic surfaces and delayed development in disease symptoms when sprayed on susceptible citrus leaves. Quantitative reverse transcription-PCR assays indicated that deletion of <italic>hrpB</italic>
reduced the expression of four type IV pili genes. The transcriptional start site of <italic>fimA</italic>
(XAC3241) was determined using rapid amplification of 5′-cDNA Ends (5′RACE). Based on the results of <italic>fimA</italic>
mRNA structure predictions, the <italic>fimA</italic>
5′ UTR may contain three different loops. HrpB may be involved in alterations to the structure of <italic>fimA</italic>
mRNA that promote the stability of <italic>fimA</italic>
RNA.</p>
</sec>
<sec><title>Conclusions</title>
<p>Our data show that <italic>hrpB</italic>
is involved in adherence of <italic>Xanthomonas citri</italic>
subsp. <italic>citri</italic>
to different surfaces. In addition, to the best of our knowledge, this is the first time that a DEAH RNA helicase has been implicated in the regulation of type IV pili in <italic>Xanthomonas</italic>
.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s12866-016-0655-1) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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