The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri
Identifieur interne : 000612 ( Pmc/Curation ); précédent : 000611; suivant : 000613The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri
Auteurs : Laís Moreira Granato ; Simone Cristina Picchi ; Maxuel De Oliveira Andrade [États-Unis] ; Marco Aurélio Takita ; Alessandra Alves De Souza ; Nian Wang [États-Unis] ; Marcos Antonio MachadoSource :
- BMC Microbiology [ 1471-2180 ] ; 2016.
Abstract
RNA helicases are enzymes that catalyze the separation of double-stranded RNA (dsRNA) using the free energy of ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects of RNA metabolism, including RNA synthesis, RNA folding, RNA-RNA interactions, RNA localization and RNA degradation. Several important bacterial DEAD/DEAH-box RNA helicases have been extensively studied. In this study, we characterize the ATP-dependent RNA helicase encoded by the
The
Our data show that
The online version of this article (doi:10.1186/s12866-016-0655-1) contains supplementary material, which is available to authorized users.
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DOI: 10.1186/s12866-016-0655-1
PubMed: 27005008
PubMed Central: 4804567
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Laís Moreira Granato<affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13083-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13083-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic></title><author><name sortKey="Granato, Lais Moreira" sort="Granato, Lais Moreira" uniqKey="Granato L" first="Laís Moreira" last="Granato">Laís Moreira Granato</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13083-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Picchi, Simone Cristina" sort="Picchi, Simone Cristina" uniqKey="Picchi S" first="Simone Cristina" last="Picchi">Simone Cristina Picchi</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Andrade, Maxuel De Oliveira" sort="Andrade, Maxuel De Oliveira" uniqKey="Andrade M" first="Maxuel De Oliveira" last="Andrade">Maxuel De Oliveira Andrade</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27005008</idno><idno type="pmc">4804567</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804567</idno><idno type="RBID">PMC:4804567</idno><idno type="doi">10.1186/s12866-016-0655-1</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000613</idno><idno type="wicri:Area/Pmc/Curation">000612</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic></title><author><name sortKey="Granato, Lais Moreira" sort="Granato, Lais Moreira" uniqKey="Granato L" first="Laís Moreira" last="Granato">Laís Moreira Granato</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13083-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Picchi, Simone Cristina" sort="Picchi, Simone Cristina" uniqKey="Picchi S" first="Simone Cristina" last="Picchi">Simone Cristina Picchi</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Andrade, Maxuel De Oliveira" sort="Andrade, Maxuel De Oliveira" uniqKey="Andrade M" first="Maxuel De Oliveira" last="Andrade">Maxuel De Oliveira Andrade</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name><affiliation wicri:level="2"><nlm:aff id="Aff3">Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name><affiliation><nlm:aff id="Aff1">Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 13490-970 Brazil</wicri:noCountry></affiliation></author></analytic><series><title level="j">BMC Microbiology</title><idno type="eISSN">1471-2180</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>RNA helicases are enzymes that catalyze the separation of double-stranded RNA (dsRNA) using the free energy of ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects of RNA metabolism, including RNA synthesis, RNA folding, RNA-RNA interactions, RNA localization and RNA degradation. Several important bacterial DEAD/DEAH-box RNA helicases have been extensively studied. In this study, we characterize the ATP-dependent RNA helicase encoded by the <italic>hrpB</italic> (XAC0293) gene using deletion and genetic complementation assays. We provide insights into the function of the <italic>hrpB</italic> gene in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> by investigating the roles of <italic>hrpB</italic> in biofilm formation on abiotic surfaces and host leaves, cell motility, host virulence of the citrus canker bacterium and growth <italic>in planta</italic>.</p></sec><sec><title>Results</title><p>The <italic>hrpB</italic> gene is highly conserved in the sequenced strains of <italic>Xanthomonas</italic>. Mutation of the <italic>hrpB</italic> gene (<italic>∆hrpB</italic>) resulted in a significant reduction in biofilms on abiotic surfaces and host leaves. <italic>∆hrpB</italic> also exhibited increased cell dispersion on solid medium plates. <italic>∆hrpB</italic> showed reduced adhesion on biotic and abiotic surfaces and delayed development in disease symptoms when sprayed on susceptible citrus leaves. Quantitative reverse transcription-PCR assays indicated that deletion of <italic>hrpB</italic> reduced the expression of four type IV pili genes. The transcriptional start site of <italic>fimA</italic> (XAC3241) was determined using rapid amplification of 5′-cDNA Ends (5′RACE). Based on the results of <italic>fimA</italic> mRNA structure predictions, the <italic>fimA</italic> 5′ UTR may contain three different loops. HrpB may be involved in alterations to the structure of <italic>fimA</italic> mRNA that promote the stability of <italic>fimA</italic> RNA.</p></sec><sec><title>Conclusions</title><p>Our data show that <italic>hrpB</italic> is involved in adherence of <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> to different surfaces. In addition, to the best of our knowledge, this is the first time that a DEAH RNA helicase has been implicated in the regulation of type IV pili in <italic>Xanthomonas</italic>.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12866-016-0655-1) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Silverman, E" uniqKey="Silverman E">E Silverman</name></author><author><name sortKey="Edwalds Gilbert, G" uniqKey="Edwalds Gilbert G">G Edwalds-Gilbert</name></author><author><name sortKey="Lin, Rj" uniqKey="Lin R">RJ Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cordin, O" uniqKey="Cordin O">O Cordin</name></author><author><name sortKey="Banroques, J" uniqKey="Banroques J">J Banroques</name></author><author><name sortKey="Tanner, Nk" uniqKey="Tanner N">NK Tanner</name></author><author><name sortKey="Linder, P" uniqKey="Linder P">P Linder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanner, Nk" uniqKey="Tanner N">NK Tanner</name></author><author><name sortKey="Linder, P" uniqKey="Linder P">P Linder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caruthers, Jm" uniqKey="Caruthers J">JM Caruthers</name></author><author><name sortKey="Mckay, Db" uniqKey="Mckay D">DB McKay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rocak, S" uniqKey="Rocak S">S Rocak</name></author><author><name sortKey="Linder, P" uniqKey="Linder P">P Linder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oun, S" uniqKey="Oun S">S Oun</name></author><author><name sortKey="Redder, P" uniqKey="Redder P">P Redder</name></author><author><name sortKey="Didier, J" uniqKey="Didier J">J Didier</name></author><author><name sortKey="Francois, P" uniqKey="Francois P">P François</name></author><author><name sortKey="Corvaglia, A" uniqKey="Corvaglia A">A Corvaglia</name></author><author><name sortKey="Buttazzoni, E" uniqKey="Buttazzoni E">E Buttazzoni</name></author><author><name sortKey="Giraud, C" uniqKey="Giraud C">C Giraud</name></author><author><name sortKey="Girard, M" uniqKey="Girard M">M Girard</name></author><author><name sortKey="Schrenzel, J" uniqKey="Schrenzel J">J Schrenzel</name></author><author><name sortKey="Linder, P" uniqKey="Linder P">P Linder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gong, Z" uniqKey="Gong Z">Z Gong</name></author><author><name sortKey="Dong, C H" uniqKey="Dong C">C-H Dong</name></author><author><name sortKey="Lee, H" uniqKey="Lee H">H Lee</name></author><author><name sortKey="Zhu, J" uniqKey="Zhu J">J Zhu</name></author><author><name sortKey="Xiong, L" uniqKey="Xiong L">L Xiong</name></author><author><name sortKey="Gong, D" uniqKey="Gong D">D Gong</name></author><author><name sortKey="Stevenson, B" uniqKey="Stevenson B">B Stevenson</name></author><author><name sortKey="Zhu, J K" uniqKey="Zhu J">J-K Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaberdin, Vr" uniqKey="Kaberdin V">VR Kaberdin</name></author><author><name sortKey="Bl Si, U" uniqKey="Bl Si U">U Bläsi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Linder, P" uniqKey="Linder P">P Linder</name></author><author><name sortKey="Jankowsky, E" uniqKey="Jankowsky E">E Jankowsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hunger, K" uniqKey="Hunger K">K Hunger</name></author><author><name sortKey="Beckering, Cl" uniqKey="Beckering C">CL Beckering</name></author><author><name sortKey="Wiegeshoff, F" uniqKey="Wiegeshoff F">F Wiegeshoff</name></author><author><name sortKey="Graumann, Pl" uniqKey="Graumann P">PL Graumann</name></author><author><name sortKey="Marahiel, Ma" uniqKey="Marahiel M">MA Marahiel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roos, S" uniqKey="Roos S">S Roos</name></author><author><name sortKey="Lindgren, S" uniqKey="Lindgren S">S Lindgren</name></author><author><name sortKey="Jonsson, H" uniqKey="Jonsson H">H Jonsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Markkula, A" uniqKey="Markkula A">A Markkula</name></author><author><name sortKey="Mattila, M" uniqKey="Mattila M">M Mattila</name></author><author><name sortKey="Lindstrom, M" uniqKey="Lindstrom M">M Lindström</name></author><author><name sortKey="Korkeala, H" uniqKey="Korkeala H">H Korkeala</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koo, Jt" uniqKey="Koo J">JT Koo</name></author><author><name sortKey="Choe, J" uniqKey="Choe J">J Choe</name></author><author><name sortKey="Moseley, Sl" uniqKey="Moseley S">SL Moseley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Butland, G" uniqKey="Butland G">G Butland</name></author><author><name sortKey="Peregrin Alvarez, Jm" uniqKey="Peregrin Alvarez J">JM Peregrin-Alvarez</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Yang, W" uniqKey="Yang W">W Yang</name></author><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salman Dilgimen, A" uniqKey="Salman Dilgimen A">A Salman-Dilgimen</name></author><author><name sortKey="Hardy, Po" uniqKey="Hardy P">PO Hardy</name></author><author><name sortKey="Dresser, Ar" uniqKey="Dresser A">AR Dresser</name></author><author><name sortKey="Chaconas, G" uniqKey="Chaconas G">G Chaconas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gottwald, Tr" uniqKey="Gottwald T">TR Gottwald</name></author><author><name sortKey="Pierce, F" uniqKey="Pierce F">F Pierce</name></author><author><name sortKey="Graham, Jh" uniqKey="Graham J">JH Graham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Graham, Jh" uniqKey="Graham J">JH Graham</name></author><author><name sortKey="Gottwal, Tr" uniqKey="Gottwal T">TR Gottwal</name></author><author><name sortKey="Cubero, J" uniqKey="Cubero J">J Cubero</name></author><author><name sortKey="Achor, Ds" uniqKey="Achor D">DS Achor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rigano, La" uniqKey="Rigano L">LA Rigano</name></author><author><name sortKey="Siciliano, F" uniqKey="Siciliano F">F Siciliano</name></author><author><name sortKey="Enrique, R" uniqKey="Enrique R">R Enrique</name></author><author><name sortKey="Sendin, L" uniqKey="Sendin L">L Sendín</name></author><author><name sortKey="Filippone, P" uniqKey="Filippone P">P Filippone</name></author><author><name sortKey="Torres, Ps" uniqKey="Torres P">PS Torres</name></author><author><name sortKey="Questa, J" uniqKey="Questa J">J Qüesta</name></author><author><name sortKey="Dow, Jm" uniqKey="Dow J">JM Dow</name></author><author><name sortKey="Castagnaro, Ap" uniqKey="Castagnaro A">AP Castagnaro</name></author><author><name sortKey="Vojnov, Aa" uniqKey="Vojnov A">AA Vojnov</name></author><author><name sortKey="Marano, Mr" uniqKey="Marano M">MR Marano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Malamud, F" uniqKey="Malamud F">F Malamud</name></author><author><name sortKey="Torres, Ps" uniqKey="Torres P">PS Torres</name></author><author><name sortKey="Roeschlin, R" uniqKey="Roeschlin R">R Roeschlin</name></author><author><name sortKey="Rigano, La" uniqKey="Rigano L">LA Rigano</name></author><author><name sortKey="Enrique, R" uniqKey="Enrique R">R Enrique</name></author><author><name sortKey="Bonomi, Hr" uniqKey="Bonomi H">HR Bonomi</name></author><author><name sortKey="Castagnaro, Ap" uniqKey="Castagnaro A">AP Castagnaro</name></author><author><name sortKey="Marano, Mr" uniqKey="Marano M">MR Marano</name></author><author><name sortKey="Vojnov, Aa" uniqKey="Vojnov A">AA Vojnov</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Costerton, Jw" uniqKey="Costerton J">JW Costerton</name></author><author><name sortKey="Lewandowski, Z" uniqKey="Lewandowski Z">Z Lewandowski</name></author><author><name sortKey="Caldwell, De" uniqKey="Caldwell D">DE Caldwell</name></author><author><name sortKey="Korber, Dr" uniqKey="Korber D">DR Korber</name></author><author><name sortKey="Lappin Scott, Hm" uniqKey="Lappin Scott H">HM Lappin-Scott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caserta, R" uniqKey="Caserta R">R Caserta</name></author><author><name sortKey="Takita, Ma" uniqKey="Takita M">MA Takita</name></author><author><name sortKey="Targon, Ml" uniqKey="Targon M">ML Targon</name></author><author><name sortKey="Rosselli Murai, Lk" uniqKey="Rosselli Murai L">LK Rosselli-Murai</name></author><author><name sortKey="De Souza, Ap" uniqKey="De Souza A">AP De Souza</name></author><author><name sortKey="Peroni, L" uniqKey="Peroni L">L Peroni</name></author><author><name sortKey="Stach Machado, Dr" uniqKey="Stach Machado D">DR Stach-Machado</name></author><author><name sortKey="Andrade, A" uniqKey="Andrade A">A Andrade</name></author><author><name sortKey="Labate, Ca" uniqKey="Labate C">CA Labate</name></author><author><name sortKey="Kitajima, Ew" uniqKey="Kitajima E">EW Kitajima</name></author><author><name sortKey="Machado, Ma" uniqKey="Machado M">MA Machado</name></author><author><name sortKey="De Souza, Aa" uniqKey="De Souza A">AA De Souza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Da Silva, Acr" uniqKey="Da Silva A">ACR da Silva</name></author><author><name sortKey="Ferro, Ja" uniqKey="Ferro J">JA Ferro</name></author><author><name sortKey="Reinach, Fc" uniqKey="Reinach F">FC Reinach</name></author><author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name></author><author><name sortKey="Furlan, Lr" uniqKey="Furlan L">LR Furlan</name></author><author><name sortKey="Quaggio, Rb" uniqKey="Quaggio R">RB Quaggio</name></author><author><name sortKey="Monteiro Vitorello, Cb" uniqKey="Monteiro Vitorello C">CB Monteiro-Vitorello</name></author><author><name sortKey="Van Sluys, Ma" uniqKey="Van Sluys M">MA Van Sluys</name></author><author><name sortKey="Almeida, Nf" uniqKey="Almeida N">NF Almeida</name></author><author><name sortKey="Alves, Lmc" uniqKey="Alves L">LMC Alves</name></author><author><name sortKey="Do Amaral, Am" uniqKey="Do Amaral A">AM do Amaral</name></author><author><name sortKey="Bertolini, Mc" uniqKey="Bertolini M">MC Bertolini</name></author><author><name sortKey="Camargo, Lea" uniqKey="Camargo L">LEA Camargo</name></author><author><name sortKey="Camarotte, G" uniqKey="Camarotte G">G Camarotte</name></author><author><name sortKey="Cannavan, F" uniqKey="Cannavan F">F Cannavan</name></author><author><name sortKey="Cardozo, J" uniqKey="Cardozo J">J Cardozo</name></author><author><name sortKey="Chambergo, F" uniqKey="Chambergo F">F Chambergo</name></author><author><name sortKey="Ciapina, Lp" uniqKey="Ciapina L">LP Ciapina</name></author><author><name sortKey="Cicarelli, Rmb" uniqKey="Cicarelli R">RMB Cicarelli</name></author><author><name sortKey="Coutinho, Ll" uniqKey="Coutinho L">LL Coutinho</name></author><author><name sortKey="Cursino Santos, Jr" uniqKey="Cursino Santos J">JR Cursino-Santos</name></author><author><name sortKey="El Dorry, H" uniqKey="El Dorry H">H El-Dorry</name></author><author><name sortKey="Faria, Jb" uniqKey="Faria J">JB Faria</name></author><author><name sortKey="Ferreira, Ajs" uniqKey="Ferreira A">AJS Ferreira</name></author><author><name sortKey="Ferreira, Rcc" uniqKey="Ferreira R">RCC Ferreira</name></author><author><name sortKey="Ferro, Mit" uniqKey="Ferro M">MIT Ferro</name></author><author><name sortKey="Formighieri, Ef" uniqKey="Formighieri E">EF Formighieri</name></author><author><name sortKey="Franco, Mc" uniqKey="Franco M">MC Franco</name></author><author><name sortKey="Greggio, Cc" uniqKey="Greggio C">CC Greggio</name></author><author><name sortKey="Gruber, A" uniqKey="Gruber A">A Gruber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dunger, G" uniqKey="Dunger G">G Dunger</name></author><author><name sortKey="Guzzo, Cr" uniqKey="Guzzo C">CR Guzzo</name></author><author><name sortKey="Andrade, Mo" uniqKey="Andrade M">MO Andrade</name></author><author><name sortKey="Jones, Jb" uniqKey="Jones J">JB Jones</name></author><author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guzzo, Cr" uniqKey="Guzzo C">CR Guzzo</name></author><author><name sortKey="Salinas, Rk" uniqKey="Salinas R">RK Salinas</name></author><author><name sortKey="Andrade, Mo" uniqKey="Andrade M">MO Andrade</name></author><author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guzzo, Cr" uniqKey="Guzzo C">CR Guzzo</name></author><author><name sortKey="Dunger, G" uniqKey="Dunger G">G Dunger</name></author><author><name sortKey="Salinas, Rk" uniqKey="Salinas R">RK Salinas</name></author><author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Finn, Rda" uniqKey="Finn R">RDA Finn</name></author><author><name sortKey="Bateman, J" uniqKey="Bateman J">J Bateman</name></author><author><name sortKey="Clements, P" uniqKey="Clements P">P Clements</name></author><author><name sortKey="Coggill, Ry" uniqKey="Coggill R">RY Coggill</name></author><author><name sortKey="Eberhardt, Sr" uniqKey="Eberhardt S">SR Eberhardt</name></author><author><name sortKey="Eddy, A" uniqKey="Eddy A">A Eddy</name></author><author><name sortKey="Heger, K" uniqKey="Heger K">K Heger</name></author><author><name sortKey="Hetherington, L" uniqKey="Hetherington L">L Hetherington</name></author><author><name sortKey="Holm, J" uniqKey="Holm J">J Holm</name></author><author><name sortKey="Mistry, Ell" uniqKey="Mistry E">ELL Mistry</name></author><author><name sortKey="Sonnhammer, J" uniqKey="Sonnhammer J">J Sonnhammer</name></author><author><name sortKey="Tate, Mrd" uniqKey="Tate M">MRD Tate</name></author><author><name sortKey="Finn, A" uniqKey="Finn A">A Finn</name></author><author><name sortKey="Bateman, J" uniqKey="Bateman J">J Bateman</name></author><author><name sortKey="Clements, P" uniqKey="Clements P">P Clements</name></author><author><name sortKey="Coggill, Ry" uniqKey="Coggill R">RY Coggill</name></author><author><name sortKey="Eberhardt, Sr" uniqKey="Eberhardt S">SR Eberhardt</name></author><author><name sortKey="Eddy, A" uniqKey="Eddy A">A Eddy</name></author><author><name sortKey="Heger, K" uniqKey="Heger K">K Heger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Y" uniqKey="Xu Y">Y Xu</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Lan, R" uniqKey="Lan R">R Lan</name></author><author><name sortKey="Xiong, Y" uniqKey="Xiong Y">Y Xiong</name></author><author><name sortKey="Ye, C" uniqKey="Ye C">C Ye</name></author><author><name sortKey="Ren, Z" uniqKey="Ren Z">Z Ren</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Zhao, A" uniqKey="Zhao A">A Zhao</name></author><author><name sortKey="Wu, Lf" uniqKey="Wu L">LF Wu</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chiang, P" uniqKey="Chiang P">P Chiang</name></author><author><name sortKey="Sampaleanu, Lm" uniqKey="Sampaleanu L">LM Sampaleanu</name></author><author><name sortKey="Ayers, M" uniqKey="Ayers M">M Ayers</name></author><author><name sortKey="Pahuta, M" uniqKey="Pahuta M">M Pahuta</name></author><author><name sortKey="Howell, Pl" uniqKey="Howell P">PL Howell</name></author><author><name sortKey="Burrows, Ll" uniqKey="Burrows L">LL Burrows</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zuker, M" uniqKey="Zuker M">M Zuker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gottig, N" uniqKey="Gottig N">N Gottig</name></author><author><name sortKey="Garavaglia, Bs" uniqKey="Garavaglia B">BS Garavaglia</name></author><author><name sortKey="Garofalo, Cg" uniqKey="Garofalo C">CG Garofalo</name></author><author><name sortKey="Orellano, Eg" uniqKey="Orellano E">EG Orellano</name></author><author><name sortKey="Ottado, J" uniqKey="Ottado J">J Ottado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Py, B" uniqKey="Py B">B Py</name></author><author><name sortKey="Higgins, Cf" uniqKey="Higgins C">CF Higgins</name></author><author><name sortKey="Krisch, Hm" uniqKey="Krisch H">HM Krisch</name></author><author><name sortKey="Carpousis, Aj" uniqKey="Carpousis A">AJ Carpousis</name></author><author><name sortKey="Carpousis, Aj" uniqKey="Carpousis A">AJ Carpousis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khemici, V" uniqKey="Khemici V">V Khemici</name></author><author><name sortKey="Toesca, I" uniqKey="Toesca I">I Toesca</name></author><author><name sortKey="Poljak, L" uniqKey="Poljak L">L Poljak</name></author><author><name sortKey="Vanzo, Nf" uniqKey="Vanzo N">NF Vanzo</name></author><author><name sortKey="Carpousis, Aj" uniqKey="Carpousis A">AJ Carpousis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Danhorn, T" uniqKey="Danhorn T">T Danhorn</name></author><author><name sortKey="Fuqua, C" uniqKey="Fuqua C">C Fuqua</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Guo, Y" uniqKey="Guo Y">Y Guo</name></author><author><name sortKey="Sagaram, Us" uniqKey="Sagaram U">US Sagaram</name></author><author><name sortKey="Kim, J" uniqKey="Kim J">J Kim</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yan, Q" uniqKey="Yan Q">Q Yan</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zimaro, T" uniqKey="Zimaro T">T Zimaro</name></author><author><name sortKey="Thomas, L" uniqKey="Thomas L">L Thomas</name></author><author><name sortKey="Marondedze, C" uniqKey="Marondedze C">C Marondedze</name></author><author><name sortKey="Sgro, Gg" uniqKey="Sgro G">GG Sgro</name></author><author><name sortKey="Garofalo, Cg" uniqKey="Garofalo C">CG Garofalo</name></author><author><name sortKey="Ficarra, Fa" uniqKey="Ficarra F">FA Ficarra</name></author><author><name sortKey="Gehring, C" uniqKey="Gehring C">C Gehring</name></author><author><name sortKey="Ottado, J" uniqKey="Ottado J">J Ottado</name></author><author><name sortKey="Gottig, N" uniqKey="Gottig N">N Gottig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gury, J" uniqKey="Gury J">J Gury</name></author><author><name sortKey="Barthelmebs, L" uniqKey="Barthelmebs L">L Barthelmebs</name></author><author><name sortKey="Cavin, Jf" uniqKey="Cavin J">JF Cavin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tu Quoc, Ph" uniqKey="Tu Quoc P">PH Tu Quoc</name></author><author><name sortKey="Genevaux, P" uniqKey="Genevaux P">P Genevaux</name></author><author><name sortKey="Pajunen, M" uniqKey="Pajunen M">M Pajunen</name></author><author><name sortKey="Savilahti, H" uniqKey="Savilahti H">H Savilahti</name></author><author><name sortKey="Georgopoulos, C" uniqKey="Georgopoulos C">C Georgopoulos</name></author><author><name sortKey="Schrenzel, J" uniqKey="Schrenzel J">J Schrenzel</name></author><author><name sortKey="Kelley, Wl" uniqKey="Kelley W">WL Kelley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Andrade, Mo" uniqKey="Andrade M">MO Andrade</name></author><author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Babitzke, P" uniqKey="Babitzke P">P Babitzke</name></author><author><name sortKey="Romeo, T" uniqKey="Romeo T">T Romeo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Richards, J" uniqKey="Richards J">J Richards</name></author><author><name sortKey="Luciano, Dj" uniqKey="Luciano D">DJ Luciano</name></author><author><name sortKey="Belasco, Jg" uniqKey="Belasco J">JG Belasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deana, A" uniqKey="Deana A">A Deana</name></author><author><name sortKey="Belasco, Jg" uniqKey="Belasco J">JG Belasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arnold, Te" uniqKey="Arnold T">TE Arnold</name></author><author><name sortKey="Yu, J" uniqKey="Yu J">J Yu</name></author><author><name sortKey="Belasco, Jg" uniqKey="Belasco J">JG Belasco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matsunaga, J" uniqKey="Matsunaga J">J Matsunaga</name></author><author><name sortKey="Simons, El" uniqKey="Simons E">EL Simons</name></author><author><name sortKey="Simons, Rw" uniqKey="Simons R">RW Simons</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hanahan, D" uniqKey="Hanahan D">D Hanahan</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="El Yacoubi, B" uniqKey="El Yacoubi B">B El Yacoubi</name></author><author><name sortKey="Brunings, Am" uniqKey="Brunings A">AM Brunings</name></author><author><name sortKey="Yuan, Q" uniqKey="Yuan Q">Q Yuan</name></author><author><name sortKey="Shankar, S" uniqKey="Shankar S">S Shankar</name></author><author><name sortKey="Gabriel, Dw" uniqKey="Gabriel D">DW Gabriel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J Sambrook</name></author><author><name sortKey="Fritsch, E" uniqKey="Fritsch E">E Fritsch</name></author><author><name sortKey="Maniatis, T" uniqKey="Maniatis T">T Maniatis</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Souza, Dp" uniqKey="Souza D">DP Souza</name></author><author><name sortKey="Andrade, Mo" uniqKey="Andrade M">MO Andrade</name></author><author><name sortKey="Alvarez Martinez, Ce" uniqKey="Alvarez Martinez C">CE Alvarez-Martinez</name></author><author><name sortKey="Arantes, Gm" uniqKey="Arantes G">GM Arantes</name></author><author><name sortKey="Farah, Cs" uniqKey="Farah C">CS Farah</name></author><author><name sortKey="Salinas, Rk" uniqKey="Salinas R">RK Salinas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">BMC Microbiol</journal-id><journal-title-group><journal-title>BMC Microbiology</journal-title></journal-title-group><issn pub-type="epub">1471-2180</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27005008</article-id><article-id pub-id-type="pmc">4804567</article-id><article-id pub-id-type="publisher-id">655</article-id><article-id pub-id-type="doi">10.1186/s12866-016-0655-1</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Granato</surname><given-names>Laís Moreira</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Picchi</surname><given-names>Simone Cristina</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Andrade</surname><given-names>Maxuel de Oliveira</given-names></name><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Takita</surname><given-names>Marco Aurélio</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>de Souza</surname><given-names>Alessandra Alves</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Nian</given-names></name><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0001-9780-3990</contrib-id><name><surname>Machado</surname><given-names>Marcos Antonio</given-names></name><address><email>marcos@centrodecitricultura.br</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><label></label>Centro de Citricultura Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis, SP 13490-970 Brazil</aff><aff id="Aff2"><label></label>Universidade Estadual de Campinas/UNICAMP, Instituto de Biologia, P.O. Box 6010, Campinas, SP 13083-970 Brazil</aff><aff id="Aff3"><label></label>Citrus Research and Educational Center, Department of Microbiology and Cell Science, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850 USA</aff></contrib-group><pub-date pub-type="epub"><day>23</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>23</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>16</volume><elocation-id>55</elocation-id><history><date date-type="received"><day>30</day><month>6</month><year>2015</year></date><date date-type="accepted"><day>2</day><month>3</month><year>2016</year></date></history><permissions><copyright-statement>© Granato et al. 2016</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>RNA helicases are enzymes that catalyze the separation of double-stranded RNA (dsRNA) using the free energy of ATP binding and hydrolysis. DEAD/DEAH families participate in many different aspects of RNA metabolism, including RNA synthesis, RNA folding, RNA-RNA interactions, RNA localization and RNA degradation. Several important bacterial DEAD/DEAH-box RNA helicases have been extensively studied. In this study, we characterize the ATP-dependent RNA helicase encoded by the <italic>hrpB</italic> (XAC0293) gene using deletion and genetic complementation assays. We provide insights into the function of the <italic>hrpB</italic> gene in <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> by investigating the roles of <italic>hrpB</italic> in biofilm formation on abiotic surfaces and host leaves, cell motility, host virulence of the citrus canker bacterium and growth <italic>in planta</italic>.</p></sec><sec><title>Results</title><p>The <italic>hrpB</italic> gene is highly conserved in the sequenced strains of <italic>Xanthomonas</italic>. Mutation of the <italic>hrpB</italic> gene (<italic>∆hrpB</italic>) resulted in a significant reduction in biofilms on abiotic surfaces and host leaves. <italic>∆hrpB</italic> also exhibited increased cell dispersion on solid medium plates. <italic>∆hrpB</italic> showed reduced adhesion on biotic and abiotic surfaces and delayed development in disease symptoms when sprayed on susceptible citrus leaves. Quantitative reverse transcription-PCR assays indicated that deletion of <italic>hrpB</italic> reduced the expression of four type IV pili genes. The transcriptional start site of <italic>fimA</italic> (XAC3241) was determined using rapid amplification of 5′-cDNA Ends (5′RACE). Based on the results of <italic>fimA</italic> mRNA structure predictions, the <italic>fimA</italic> 5′ UTR may contain three different loops. HrpB may be involved in alterations to the structure of <italic>fimA</italic> mRNA that promote the stability of <italic>fimA</italic> RNA.</p></sec><sec><title>Conclusions</title><p>Our data show that <italic>hrpB</italic> is involved in adherence of <italic>Xanthomonas citri</italic> subsp. <italic>citri</italic> to different surfaces. In addition, to the best of our knowledge, this is the first time that a DEAH RNA helicase has been implicated in the regulation of type IV pili in <italic>Xanthomonas</italic>.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12866-016-0655-1) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>RNA helicase</kwd><kwd><italic>Xanthomonas citri</italic></kwd><kwd>Biofilm</kwd><kwd>Citrus canker</kwd><kwd>Type IV pili</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2016</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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