Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.
Identifieur interne : 001673 ( Ncbi/Checkpoint ); précédent : 001672; suivant : 001674Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.
Auteurs : Nadiezda C. Maas [Brésil] ; Ana Helena P. Gracher ; Guilherme L. Sassaki ; Philip A J. Gorin ; Marcello Iacomini ; Thales R. CiprianiSource :
- Carbohydrate polymers [ 1879-1344 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Anticoagulants (chemistry), Anticoagulants (isolation & purification), Anticoagulants (pharmacology), Blood Coagulation (drug effects), Citrus sinensis (chemistry), Female, Fibrinolytic Agents (chemistry), Fibrinolytic Agents (isolation & purification), Fibrinolytic Agents (pharmacology), Male, Pectins (chemistry), Pectins (isolation & purification), Pectins (pharmacology), Polysaccharides (chemistry), Polysaccharides (isolation & purification), Polysaccharides (pharmacology), Rats, Rats, Wistar.
- MESH :
- chemical , chemistry : Anticoagulants, Fibrinolytic Agents, Pectins, Polysaccharides.
- chemical , isolation & purification : Anticoagulants, Fibrinolytic Agents, Pectins, Polysaccharides.
- chemical , pharmacology : Anticoagulants, Fibrinolytic Agents, Pectins, Polysaccharides.
- chemistry : Citrus sinensis.
- drug effects : Blood Coagulation.
- Animals, Female, Male, Rats, Rats, Wistar.
Abstract
Citrus pectin (CP), a polysaccharide composed of [→4)-α-D-GalpA-(1→]n, was submitted to one or four carboxy-reduction cycles, resulting in CP-CR1 and CP-CR4, which had 40% and 2% of GalpA units, respectively. The polysaccharides were chemically sulfated and their anticoagulant and antithrombotic effects determined. Sulfated polysaccharides (CP-S, CP-CR1S and CP-CR4S) had different anticoagulant activities, doubling APTT at concentrations of 28.7, 13.2, and 4.9 μg/ml respectively. CP-CR1S and CP-CR4S also showed antithrombotic activity in vivo with ED50 of 3.01 and 1.70 mg/kg, respectively. Like heparin, they inhibited thrombin by a mechanism dependent on AT and HCII. Their hemorrhagic potential was also similar to that of heparin. According to methylation analysis, 91.1% and 50.2% of 6-O-position in CP-CR4S and CP-CR1S were sulfated, respectively. Therefore, substitution of carboxyl groups by sulfate esters in these polysaccharides increases the anticoagulant and antithrombotic effects.
DOI: 10.1016/j.carbpol.2012.03.070
PubMed: 24750917
Affiliations:
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Gracher</name></author><author><name sortKey="Sassaki, Guilherme L" sort="Sassaki, Guilherme L" uniqKey="Sassaki G" first="Guilherme L" last="Sassaki">Guilherme L. Sassaki</name></author><author><name sortKey="Gorin, Philip A J" sort="Gorin, Philip A J" uniqKey="Gorin P" first="Philip A J" last="Gorin">Philip A J. Gorin</name></author><author><name sortKey="Iacomini, Marcello" sort="Iacomini, Marcello" uniqKey="Iacomini M" first="Marcello" last="Iacomini">Marcello Iacomini</name></author><author><name sortKey="Cipriani, Thales R" sort="Cipriani, Thales R" uniqKey="Cipriani T" first="Thales R" last="Cipriani">Thales R. Cipriani</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:24750917</idno><idno type="pmid">24750917</idno><idno type="doi">10.1016/j.carbpol.2012.03.070</idno><idno type="wicri:Area/PubMed/Corpus">000589</idno><idno type="wicri:Area/PubMed/Curation">000589</idno><idno type="wicri:Area/PubMed/Checkpoint">000589</idno><idno type="wicri:Area/Ncbi/Merge">001673</idno><idno type="wicri:Area/Ncbi/Curation">001673</idno><idno type="wicri:Area/Ncbi/Checkpoint">001673</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.</title><author><name sortKey="Maas, Nadiezda C" sort="Maas, Nadiezda C" uniqKey="Maas N" first="Nadiezda C" last="Maas">Nadiezda C. Maas</name><affiliation wicri:level="2"><nlm:affiliation>Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR</wicri:regionArea><placeName><region type="state">Paraná (État)</region></placeName></affiliation></author><author><name sortKey="Gracher, Ana Helena P" sort="Gracher, Ana Helena P" uniqKey="Gracher A" first="Ana Helena P" last="Gracher">Ana Helena P. Gracher</name></author><author><name sortKey="Sassaki, Guilherme L" sort="Sassaki, Guilherme L" uniqKey="Sassaki G" first="Guilherme L" last="Sassaki">Guilherme L. Sassaki</name></author><author><name sortKey="Gorin, Philip A J" sort="Gorin, Philip A J" uniqKey="Gorin P" first="Philip A J" last="Gorin">Philip A J. Gorin</name></author><author><name sortKey="Iacomini, Marcello" sort="Iacomini, Marcello" uniqKey="Iacomini M" first="Marcello" last="Iacomini">Marcello Iacomini</name></author><author><name sortKey="Cipriani, Thales R" sort="Cipriani, Thales R" uniqKey="Cipriani T" first="Thales R" last="Cipriani">Thales R. Cipriani</name></author></analytic><series><title level="j">Carbohydrate polymers</title><idno type="eISSN">1879-1344</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Anticoagulants (chemistry)</term><term>Anticoagulants (isolation & purification)</term><term>Anticoagulants (pharmacology)</term><term>Blood Coagulation (drug effects)</term><term>Citrus sinensis (chemistry)</term><term>Female</term><term>Fibrinolytic Agents (chemistry)</term><term>Fibrinolytic Agents (isolation & purification)</term><term>Fibrinolytic Agents (pharmacology)</term><term>Male</term><term>Pectins (chemistry)</term><term>Pectins (isolation & purification)</term><term>Pectins (pharmacology)</term><term>Polysaccharides (chemistry)</term><term>Polysaccharides (isolation & purification)</term><term>Polysaccharides (pharmacology)</term><term>Rats</term><term>Rats, Wistar</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anticoagulants</term><term>Fibrinolytic Agents</term><term>Pectins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Anticoagulants</term><term>Fibrinolytic Agents</term><term>Pectins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anticoagulants</term><term>Fibrinolytic Agents</term><term>Pectins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Blood Coagulation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Male</term><term>Rats</term><term>Rats, Wistar</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus pectin (CP), a polysaccharide composed of [→4)-α-D-GalpA-(1→]n, was submitted to one or four carboxy-reduction cycles, resulting in CP-CR1 and CP-CR4, which had 40% and 2% of GalpA units, respectively. The polysaccharides were chemically sulfated and their anticoagulant and antithrombotic effects determined. Sulfated polysaccharides (CP-S, CP-CR1S and CP-CR4S) had different anticoagulant activities, doubling APTT at concentrations of 28.7, 13.2, and 4.9 μg/ml respectively. CP-CR1S and CP-CR4S also showed antithrombotic activity in vivo with ED50 of 3.01 and 1.70 mg/kg, respectively. Like heparin, they inhibited thrombin by a mechanism dependent on AT and HCII. Their hemorrhagic potential was also similar to that of heparin. According to methylation analysis, 91.1% and 50.2% of 6-O-position in CP-CR4S and CP-CR1S were sulfated, respectively. Therefore, substitution of carboxyl groups by sulfate esters in these polysaccharides increases the anticoagulant and antithrombotic effects.</div></front></TEI><affiliations><list><country><li>Brésil</li></country><region><li>Paraná (État)</li></region></list><tree><noCountry><name sortKey="Cipriani, Thales R" sort="Cipriani, Thales R" uniqKey="Cipriani T" first="Thales R" last="Cipriani">Thales R. Cipriani</name><name sortKey="Gorin, Philip A J" sort="Gorin, Philip A J" uniqKey="Gorin P" first="Philip A J" last="Gorin">Philip A J. Gorin</name><name sortKey="Gracher, Ana Helena P" sort="Gracher, Ana Helena P" uniqKey="Gracher A" first="Ana Helena P" last="Gracher">Ana Helena P. Gracher</name><name sortKey="Iacomini, Marcello" sort="Iacomini, Marcello" uniqKey="Iacomini M" first="Marcello" last="Iacomini">Marcello Iacomini</name><name sortKey="Sassaki, Guilherme L" sort="Sassaki, Guilherme L" uniqKey="Sassaki G" first="Guilherme L" last="Sassaki">Guilherme L. Sassaki</name></noCountry><country name="Brésil"><region name="Paraná (État)"><name sortKey="Maas, Nadiezda C" sort="Maas, Nadiezda C" uniqKey="Maas N" first="Nadiezda C" last="Maas">Nadiezda C. Maas</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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