Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.
Identifieur interne : 001673 ( Ncbi/Merge ); précédent : 001672; suivant : 001674Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.
Auteurs : Nadiezda C. Maas [Brésil] ; Ana Helena P. Gracher ; Guilherme L. Sassaki ; Philip A J. Gorin ; Marcello Iacomini ; Thales R. CiprianiSource :
- Carbohydrate polymers [ 1879-1344 ] ; 2012.
English descriptors
- KwdEn :
- Animals, Anticoagulants (chemistry), Anticoagulants (isolation & purification), Anticoagulants (pharmacology), Blood Coagulation (drug effects), Citrus sinensis (chemistry), Female, Fibrinolytic Agents (chemistry), Fibrinolytic Agents (isolation & purification), Fibrinolytic Agents (pharmacology), Male, Pectins (chemistry), Pectins (isolation & purification), Pectins (pharmacology), Polysaccharides (chemistry), Polysaccharides (isolation & purification), Polysaccharides (pharmacology), Rats, Rats, Wistar.
- MESH :
- chemical , chemistry : Anticoagulants, Fibrinolytic Agents, Pectins, Polysaccharides.
- chemical , isolation & purification : Anticoagulants, Fibrinolytic Agents, Pectins, Polysaccharides.
- chemical , pharmacology : Anticoagulants, Fibrinolytic Agents, Pectins, Polysaccharides.
- chemistry : Citrus sinensis.
- drug effects : Blood Coagulation.
- Animals, Female, Male, Rats, Rats, Wistar.
Abstract
Citrus pectin (CP), a polysaccharide composed of [→4)-α-D-GalpA-(1→]n, was submitted to one or four carboxy-reduction cycles, resulting in CP-CR1 and CP-CR4, which had 40% and 2% of GalpA units, respectively. The polysaccharides were chemically sulfated and their anticoagulant and antithrombotic effects determined. Sulfated polysaccharides (CP-S, CP-CR1S and CP-CR4S) had different anticoagulant activities, doubling APTT at concentrations of 28.7, 13.2, and 4.9 μg/ml respectively. CP-CR1S and CP-CR4S also showed antithrombotic activity in vivo with ED50 of 3.01 and 1.70 mg/kg, respectively. Like heparin, they inhibited thrombin by a mechanism dependent on AT and HCII. Their hemorrhagic potential was also similar to that of heparin. According to methylation analysis, 91.1% and 50.2% of 6-O-position in CP-CR4S and CP-CR1S were sulfated, respectively. Therefore, substitution of carboxyl groups by sulfate esters in these polysaccharides increases the anticoagulant and antithrombotic effects.
DOI: 10.1016/j.carbpol.2012.03.070
PubMed: 24750917
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000589
- to stream PubMed, to step Curation: 000589
- to stream PubMed, to step Checkpoint: 000589
Links to Exploration step
pubmed:24750917Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.</title><author><name sortKey="Maas, Nadiezda C" sort="Maas, Nadiezda C" uniqKey="Maas N" first="Nadiezda C" last="Maas">Nadiezda C. Maas</name><affiliation wicri:level="2"><nlm:affiliation>Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR</wicri:regionArea><placeName><region type="state">Paraná (État)</region></placeName></affiliation></author><author><name sortKey="Gracher, Ana Helena P" sort="Gracher, Ana Helena P" uniqKey="Gracher A" first="Ana Helena P" last="Gracher">Ana Helena P. Gracher</name></author><author><name sortKey="Sassaki, Guilherme L" sort="Sassaki, Guilherme L" uniqKey="Sassaki G" first="Guilherme L" last="Sassaki">Guilherme L. Sassaki</name></author><author><name sortKey="Gorin, Philip A J" sort="Gorin, Philip A J" uniqKey="Gorin P" first="Philip A J" last="Gorin">Philip A J. Gorin</name></author><author><name sortKey="Iacomini, Marcello" sort="Iacomini, Marcello" uniqKey="Iacomini M" first="Marcello" last="Iacomini">Marcello Iacomini</name></author><author><name sortKey="Cipriani, Thales R" sort="Cipriani, Thales R" uniqKey="Cipriani T" first="Thales R" last="Cipriani">Thales R. Cipriani</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:24750917</idno><idno type="pmid">24750917</idno><idno type="doi">10.1016/j.carbpol.2012.03.070</idno><idno type="wicri:Area/PubMed/Corpus">000589</idno><idno type="wicri:Area/PubMed/Curation">000589</idno><idno type="wicri:Area/PubMed/Checkpoint">000589</idno><idno type="wicri:Area/Ncbi/Merge">001673</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.</title><author><name sortKey="Maas, Nadiezda C" sort="Maas, Nadiezda C" uniqKey="Maas N" first="Nadiezda C" last="Maas">Nadiezda C. Maas</name><affiliation wicri:level="2"><nlm:affiliation>Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR</wicri:regionArea><placeName><region type="state">Paraná (État)</region></placeName></affiliation></author><author><name sortKey="Gracher, Ana Helena P" sort="Gracher, Ana Helena P" uniqKey="Gracher A" first="Ana Helena P" last="Gracher">Ana Helena P. Gracher</name></author><author><name sortKey="Sassaki, Guilherme L" sort="Sassaki, Guilherme L" uniqKey="Sassaki G" first="Guilherme L" last="Sassaki">Guilherme L. Sassaki</name></author><author><name sortKey="Gorin, Philip A J" sort="Gorin, Philip A J" uniqKey="Gorin P" first="Philip A J" last="Gorin">Philip A J. Gorin</name></author><author><name sortKey="Iacomini, Marcello" sort="Iacomini, Marcello" uniqKey="Iacomini M" first="Marcello" last="Iacomini">Marcello Iacomini</name></author><author><name sortKey="Cipriani, Thales R" sort="Cipriani, Thales R" uniqKey="Cipriani T" first="Thales R" last="Cipriani">Thales R. Cipriani</name></author></analytic><series><title level="j">Carbohydrate polymers</title><idno type="eISSN">1879-1344</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Anticoagulants (chemistry)</term><term>Anticoagulants (isolation & purification)</term><term>Anticoagulants (pharmacology)</term><term>Blood Coagulation (drug effects)</term><term>Citrus sinensis (chemistry)</term><term>Female</term><term>Fibrinolytic Agents (chemistry)</term><term>Fibrinolytic Agents (isolation & purification)</term><term>Fibrinolytic Agents (pharmacology)</term><term>Male</term><term>Pectins (chemistry)</term><term>Pectins (isolation & purification)</term><term>Pectins (pharmacology)</term><term>Polysaccharides (chemistry)</term><term>Polysaccharides (isolation & purification)</term><term>Polysaccharides (pharmacology)</term><term>Rats</term><term>Rats, Wistar</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anticoagulants</term><term>Fibrinolytic Agents</term><term>Pectins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Anticoagulants</term><term>Fibrinolytic Agents</term><term>Pectins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anticoagulants</term><term>Fibrinolytic Agents</term><term>Pectins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Blood Coagulation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Male</term><term>Rats</term><term>Rats, Wistar</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus pectin (CP), a polysaccharide composed of [→4)-α-D-GalpA-(1→]n, was submitted to one or four carboxy-reduction cycles, resulting in CP-CR1 and CP-CR4, which had 40% and 2% of GalpA units, respectively. The polysaccharides were chemically sulfated and their anticoagulant and antithrombotic effects determined. Sulfated polysaccharides (CP-S, CP-CR1S and CP-CR4S) had different anticoagulant activities, doubling APTT at concentrations of 28.7, 13.2, and 4.9 μg/ml respectively. CP-CR1S and CP-CR4S also showed antithrombotic activity in vivo with ED50 of 3.01 and 1.70 mg/kg, respectively. Like heparin, they inhibited thrombin by a mechanism dependent on AT and HCII. Their hemorrhagic potential was also similar to that of heparin. According to methylation analysis, 91.1% and 50.2% of 6-O-position in CP-CR4S and CP-CR1S were sulfated, respectively. Therefore, substitution of carboxyl groups by sulfate esters in these polysaccharides increases the anticoagulant and antithrombotic effects.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24750917</PMID><DateCreated><Year>2014</Year><Month>4</Month><Day>22</Day></DateCreated><DateCompleted><Year>2015</Year><Month>10</Month><Day>13</Day></DateCompleted><DateRevised><Year>2014</Year><Month>4</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1344</ISSN><JournalIssue CitedMedium="Internet"><Volume>89</Volume><Issue>4</Issue><PubDate><Year>2012</Year><Month>Aug</Month><Day>1</Day></PubDate></JournalIssue><Title>Carbohydrate polymers</Title><ISOAbbreviation>Carbohydr Polym</ISOAbbreviation></Journal><ArticleTitle>Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.</ArticleTitle><Pagination><MedlinePgn>1081-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.carbpol.2012.03.070</ELocationID><Abstract><AbstractText>Citrus pectin (CP), a polysaccharide composed of [→4)-α-D-GalpA-(1→]n, was submitted to one or four carboxy-reduction cycles, resulting in CP-CR1 and CP-CR4, which had 40% and 2% of GalpA units, respectively. The polysaccharides were chemically sulfated and their anticoagulant and antithrombotic effects determined. Sulfated polysaccharides (CP-S, CP-CR1S and CP-CR4S) had different anticoagulant activities, doubling APTT at concentrations of 28.7, 13.2, and 4.9 μg/ml respectively. CP-CR1S and CP-CR4S also showed antithrombotic activity in vivo with ED50 of 3.01 and 1.70 mg/kg, respectively. Like heparin, they inhibited thrombin by a mechanism dependent on AT and HCII. Their hemorrhagic potential was also similar to that of heparin. According to methylation analysis, 91.1% and 50.2% of 6-O-position in CP-CR4S and CP-CR1S were sulfated, respectively. Therefore, substitution of carboxyl groups by sulfate esters in these polysaccharides increases the anticoagulant and antithrombotic effects.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Maas</LastName><ForeName>Nadiezda C</ForeName><Initials>NC</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gracher</LastName><ForeName>Ana Helena P</ForeName><Initials>AH</Initials></Author><Author ValidYN="Y"><LastName>Sassaki</LastName><ForeName>Guilherme L</ForeName><Initials>GL</Initials></Author><Author ValidYN="Y"><LastName>Gorin</LastName><ForeName>Philip A J</ForeName><Initials>PA</Initials></Author><Author ValidYN="Y"><LastName>Iacomini</LastName><ForeName>Marcello</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Cipriani</LastName><ForeName>Thales R</ForeName><Initials>TR</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>Apr</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Carbohydr Polym</MedlineTA><NlmUniqueID>8307156</NlmUniqueID><ISSNLinking>0144-8617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000925">Anticoagulants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005343">Fibrinolytic Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010368">Pectins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9000-69-5</RegistryNumber><NameOfSubstance UI="C030121">pectin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000925" MajorTopicYN="Y">Anticoagulants</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001777" MajorTopicYN="N">Blood Coagulation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005343" MajorTopicYN="Y">Fibrinolytic Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010368" MajorTopicYN="Y">Pectins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="Y">Polysaccharides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017208" MajorTopicYN="N">Rats, Wistar</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>12</Month><Day>9</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>3</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>3</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>4</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>10</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24750917</ArticleId><ArticleId IdType="pii">S0144-8617(12)00298-6</ArticleId><ArticleId IdType="doi">10.1016/j.carbpol.2012.03.070</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>Paraná (État)</li></region></list><tree><noCountry><name sortKey="Cipriani, Thales R" sort="Cipriani, Thales R" uniqKey="Cipriani T" first="Thales R" last="Cipriani">Thales R. Cipriani</name><name sortKey="Gorin, Philip A J" sort="Gorin, Philip A J" uniqKey="Gorin P" first="Philip A J" last="Gorin">Philip A J. Gorin</name><name sortKey="Gracher, Ana Helena P" sort="Gracher, Ana Helena P" uniqKey="Gracher A" first="Ana Helena P" last="Gracher">Ana Helena P. Gracher</name><name sortKey="Iacomini, Marcello" sort="Iacomini, Marcello" uniqKey="Iacomini M" first="Marcello" last="Iacomini">Marcello Iacomini</name><name sortKey="Sassaki, Guilherme L" sort="Sassaki, Guilherme L" uniqKey="Sassaki G" first="Guilherme L" last="Sassaki">Guilherme L. Sassaki</name></noCountry><country name="Brésil"><region name="Paraná (État)"><name sortKey="Maas, Nadiezda C" sort="Maas, Nadiezda C" uniqKey="Maas N" first="Nadiezda C" last="Maas">Nadiezda C. Maas</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001673 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001673 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:24750917
|texte= Sulfation pattern of citrus pectin and its carboxy-reduced derivatives: influence on anticoagulant and antithrombotic effects.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:24750917" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |