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In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant

Identifieur interne : 001396 ( Ncbi/Checkpoint ); précédent : 001395; suivant : 001397

In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant

Auteurs : Junaid Aslam [Inde] ; Abdul Mujib [Inde] ; Maheshwar Prasad Sharma [Inde]

Source :

RBID : PMC:3730903

Abstract

A protocol has been developed for in vitro plant regeneration from a nodal explant of Dracaena sanderiana Sander ex Mast. Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 μM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 μM N6-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when added with 7.38 μM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1). In vitro and ex vitro raised plantlets were used for acclimatization. More than 90% of the plantlets was successfully acclimatized and established in plastic pots. Ex vitro transferred plantlets were normal without any phenotypic aberrations.


Url:
DOI: 10.1016/j.sjbs.2012.11.005
PubMed: 23961221
PubMed Central: 3730903


Affiliations:


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PMC:3730903

Le document en format XML

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micropropagation of
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Sander ex Mast: An important indoor ornamental plant</title>
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micropropagation of
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Sander ex Mast: An important indoor ornamental plant</title>
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<p>A protocol has been developed for
<italic>in vitro</italic>
plant regeneration from a nodal explant of
<italic>Dracaena sanderiana</italic>
Sander ex Mast. Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 μM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 μM N
<sup>6</sup>
-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when added with 7.38 μM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1).
<italic>In vitro</italic>
and
<italic>ex vitro</italic>
raised plantlets were used for acclimatization. More than 90% of the plantlets was successfully acclimatized and established in plastic pots.
<italic>Ex vitro</italic>
transferred plantlets were normal without any phenotypic aberrations.</p>
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<name sortKey="Sharma, Maheshwar Prasad" sort="Sharma, Maheshwar Prasad" uniqKey="Sharma M" first="Maheshwar Prasad" last="Sharma">Maheshwar Prasad Sharma</name>
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