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In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant

Identifieur interne : 000D47 ( Pmc/Corpus ); précédent : 000D46; suivant : 000D48

In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant

Auteurs : Junaid Aslam ; Abdul Mujib ; Maheshwar Prasad Sharma

Source :

Saudi Journal of Biological Sciences [ 1319-562X ] ; 2012.

RBID : PMC:3730903

Abstract

A protocol has been developed for in vitro plant regeneration from a nodal explant of Dracaena sanderiana Sander ex Mast. Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 μM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 μM N⁶-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when added with 7.38 μM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1). In vitro and ex vitro raised plantlets were used for acclimatization. More than 90% of the plantlets was successfully acclimatized and established in plastic pots. Ex vitro transferred plantlets were normal without any phenotypic aberrations.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730903

DOI: 10.1016/j.sjbs.2012.11.005
PubMed: 23961221
PubMed Central: 3730903

Links to Exploration step

PMC:3730903

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In vitro</italic>
 micropropagation of <italic>Dracaena sanderiana</italic>
 Sander ex Mast: An important indoor ornamental plant</title>
<author><name sortKey="Aslam, Junaid" sort="Aslam, Junaid" uniqKey="Aslam J" first="Junaid" last="Aslam">Junaid Aslam</name>
<affiliation><nlm:aff id="aff1">Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110 062, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mujib, Abdul" sort="Mujib, Abdul" uniqKey="Mujib A" first="Abdul" last="Mujib">Abdul Mujib</name>
<affiliation><nlm:aff id="aff1">Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sharma, Maheshwar Prasad" sort="Sharma, Maheshwar Prasad" uniqKey="Sharma M" first="Maheshwar Prasad" last="Sharma">Maheshwar Prasad Sharma</name>
<affiliation><nlm:aff id="aff1">Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India</nlm:aff>
</affiliation>
</author>
</titleStmt>
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<idno type="pmid">23961221</idno>
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<idno type="doi">10.1016/j.sjbs.2012.11.005</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">000D47</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In vitro</italic>
 micropropagation of <italic>Dracaena sanderiana</italic>
 Sander ex Mast: An important indoor ornamental plant</title>
<author><name sortKey="Aslam, Junaid" sort="Aslam, Junaid" uniqKey="Aslam J" first="Junaid" last="Aslam">Junaid Aslam</name>
<affiliation><nlm:aff id="aff1">Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110 062, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mujib, Abdul" sort="Mujib, Abdul" uniqKey="Mujib A" first="Abdul" last="Mujib">Abdul Mujib</name>
<affiliation><nlm:aff id="aff1">Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sharma, Maheshwar Prasad" sort="Sharma, Maheshwar Prasad" uniqKey="Sharma M" first="Maheshwar Prasad" last="Sharma">Maheshwar Prasad Sharma</name>
<affiliation><nlm:aff id="aff1">Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Saudi Journal of Biological Sciences</title>
<idno type="ISSN">1319-562X</idno>
<idno type="eISSN">2213-7106</idno>
<imprint><date when="2012">2012</date>
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</fileDesc>
<profileDesc><textClass></textClass>
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<front><div type="abstract" xml:lang="en"><p>A protocol has been developed for <italic>in vitro</italic>
 plant regeneration from a nodal explant of <italic>Dracaena sanderiana</italic>
 Sander ex Mast. Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 μM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 μM N<sup>6</sup>
-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when added with 7.38 μM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1). <italic>In vitro</italic>
 and <italic>ex vitro</italic>
 raised plantlets were used for acclimatization. More than 90% of the plantlets was successfully acclimatized and established in plastic pots. <italic>Ex vitro</italic>
 transferred plantlets were normal without any phenotypic aberrations.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Saudi J Biol Sci</journal-id>
<journal-title-group><journal-title>Saudi Journal of Biological Sciences</journal-title>
</journal-title-group>
<issn pub-type="ppub">1319-562X</issn>
<issn pub-type="epub">2213-7106</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23961221</article-id>
<article-id pub-id-type="pmc">3730903</article-id>
<article-id pub-id-type="publisher-id">SJBS200</article-id>
<article-id pub-id-type="doi">10.1016/j.sjbs.2012.11.005</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>In vitro</italic>
 micropropagation of <italic>Dracaena sanderiana</italic>
 Sander ex Mast: An important indoor ornamental plant</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Aslam</surname>
<given-names>Junaid</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="aff2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mujib</surname>
<given-names>Abdul</given-names>
</name>
<email>amujib3@yahoo.co.in</email>
<xref rid="aff1" ref-type="aff">a</xref>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sharma</surname>
<given-names>Maheshwar Prasad</given-names>
</name>
<xref rid="aff1" ref-type="aff">a</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>a</label>
Cellular Differentiation and Molecular Genetics Section, Department of Botany, Hamdard University, New Delhi 110 062, India</aff>
<aff id="aff2"><label>b</label>
Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110 062, India</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Corresponding author. Tel.: +9109868112120; fax: +911126059683. <email>amujib3@yahoo.co.in</email>
</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>17</day>
<month>11</month>
<year>2012</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
      <pub-date pub-type="epub"><day>17</day>
<month>11</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="ppub"><month>1</month>
<year>2013</year>
</pub-date>
<volume>20</volume>
<issue>1</issue>
<fpage>63</fpage>
<lpage>68</lpage>
<history><date date-type="received"><day>1</day>
<month>8</month>
<year>2012</year>
</date>
<date date-type="rev-recd"><day>4</day>
<month>11</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>6</day>
<month>11</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>© 2013 King Saud University. Production and Hosting by Elsevier B.V. All rights reserved.</copyright-statement>
<copyright-year>2012</copyright-year>
<copyright-holder></copyright-holder>
</permissions>
<abstract><p>A protocol has been developed for <italic>in vitro</italic>
 plant regeneration from a nodal explant of <italic>Dracaena sanderiana</italic>
 Sander ex Mast. Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 μM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 μM N<sup>6</sup>
-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when added with 7.38 μM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1). <italic>In vitro</italic>
 and <italic>ex vitro</italic>
 raised plantlets were used for acclimatization. More than 90% of the plantlets was successfully acclimatized and established in plastic pots. <italic>Ex vitro</italic>
 transferred plantlets were normal without any phenotypic aberrations.</p>
</abstract>
<kwd-group><title>Keywords</title>
<kwd><italic>In vitro rooting</italic>
</kwd>
<kwd><italic>Dracaena sanderiana</italic>
</kwd>
<kwd>Indoor ornamental</kwd>
<kwd>Plant regeneration</kwd>
<kwd>Shoot multiplication</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>

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In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant

In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant

Source :

Abstract

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