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Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA]

Identifieur interne : 000E47 ( Ncbi/Checkpoint ); précédent : 000E46; suivant : 000E48

Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA]

Auteurs : Liang Sun ; Yufei Sun ; Mei Zhang ; Ling Wang ; Jie Ren ; Mengmeng Cui ; Yanping Wang ; Kai Ji ; Ping Li ; Qian Li ; Pei Chen ; Shengjie Dai ; Chaorui Duan ; Yan Wu ; Ping Leng

Source :

Plant Physiology [ 0032-0889 ] ; 2012.

RBID : PMC:3252109

Abstract

Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (Solanum lycopersicum) fruit ripening, we suppressed SlNCED1, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of ABA. To suppress SlNCED1 specifically in tomato fruits, and thus avoid the pleiotropic phenotypes associated with ABA deficiency, we used an RNA interference construct driven by the fruit-specific E8 promoter. ABA accumulation and SlNCED1 transcript levels in the transgenic fruit were down-regulated to between 20% and 50% of the levels measured in the control fruit. This significant reduction in NCED activity led to a down-regulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase (SlPG), pectin methyl esterase (SlPME), β-galactosidase precursor mRNA (SlTBG), xyloglucan endotransglycosylase (SlXET), endo-1,4-β-cellulose (SlCels), and expansin (SlExp). This resulted in an increased accumulation of pectin during ripening. In turn, this led to a significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%. In conclusion, ABA affects cell wall catabolism during tomato fruit ripening via down-regulation of the expression of major catabolic genes (SlPG, SlPME, SlTBG, SlXET, SlCels, and SlExp).

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252109

DOI: 10.1104/pp.111.186866
PubMed: 22108525
PubMed Central: 3252109

Affiliations:

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PMC:3252109

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Suppression of 9<italic>-</italic>
cis<italic>-</italic>
Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn3"><sup>[W]</sup>
</xref>
<xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup>
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<author><name sortKey="Sun, Liang" sort="Sun, Liang" uniqKey="Sun L" first="Liang" last="Sun">Liang Sun</name>
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<author><name sortKey="Sun, Yufei" sort="Sun, Yufei" uniqKey="Sun Y" first="Yufei" last="Sun">Yufei Sun</name>
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<author><name sortKey="Zhang, Mei" sort="Zhang, Mei" uniqKey="Zhang M" first="Mei" last="Zhang">Mei Zhang</name>
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<author><name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name>
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<author><name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
</author>
<author><name sortKey="Cui, Mengmeng" sort="Cui, Mengmeng" uniqKey="Cui M" first="Mengmeng" last="Cui">Mengmeng Cui</name>
</author>
<author><name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name>
</author>
<author><name sortKey="Ji, Kai" sort="Ji, Kai" uniqKey="Ji K" first="Kai" last="Ji">Kai Ji</name>
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<author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
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<author><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name>
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<author><name sortKey="Chen, Pei" sort="Chen, Pei" uniqKey="Chen P" first="Pei" last="Chen">Pei Chen</name>
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<author><name sortKey="Dai, Shengjie" sort="Dai, Shengjie" uniqKey="Dai S" first="Shengjie" last="Dai">Shengjie Dai</name>
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<author><name sortKey="Duan, Chaorui" sort="Duan, Chaorui" uniqKey="Duan C" first="Chaorui" last="Duan">Chaorui Duan</name>
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<author><name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name>
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<author><name sortKey="Leng, Ping" sort="Leng, Ping" uniqKey="Leng P" first="Ping" last="Leng">Ping Leng</name>
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Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
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<xref ref-type="author-notes" rid="fn3"><sup>[W]</sup>
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<xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup>
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<author><name sortKey="Sun, Liang" sort="Sun, Liang" uniqKey="Sun L" first="Liang" last="Sun">Liang Sun</name>
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<author><name sortKey="Zhang, Mei" sort="Zhang, Mei" uniqKey="Zhang M" first="Mei" last="Zhang">Mei Zhang</name>
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<author><name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name>
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<author><name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
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<author><name sortKey="Cui, Mengmeng" sort="Cui, Mengmeng" uniqKey="Cui M" first="Mengmeng" last="Cui">Mengmeng Cui</name>
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<author><name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name>
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<author><name sortKey="Ji, Kai" sort="Ji, Kai" uniqKey="Ji K" first="Kai" last="Ji">Kai Ji</name>
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<author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
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<author><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name>
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<author><name sortKey="Chen, Pei" sort="Chen, Pei" uniqKey="Chen P" first="Pei" last="Chen">Pei Chen</name>
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<author><name sortKey="Dai, Shengjie" sort="Dai, Shengjie" uniqKey="Dai S" first="Shengjie" last="Dai">Shengjie Dai</name>
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<author><name sortKey="Duan, Chaorui" sort="Duan, Chaorui" uniqKey="Duan C" first="Chaorui" last="Duan">Chaorui Duan</name>
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<author><name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name>
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<author><name sortKey="Leng, Ping" sort="Leng, Ping" uniqKey="Leng P" first="Ping" last="Leng">Ping Leng</name>
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<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
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<front><div type="abstract" xml:lang="en"><p>Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (<italic>Solanum lycopersicum</italic>
) fruit ripening, we suppressed <italic>SlNCED1</italic>
, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of ABA. To suppress <italic>SlNCED1</italic>
 specifically in tomato fruits, and thus avoid the pleiotropic phenotypes associated with ABA deficiency, we used an RNA interference construct driven by the fruit-specific <italic>E8</italic>
 promoter. ABA accumulation and <italic>SlNCED1</italic>
 transcript levels in the transgenic fruit were down-regulated to between 20% and 50% of the levels measured in the control fruit. This significant reduction in NCED activity led to a down-regulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase (<italic>SlPG</italic>
), pectin methyl esterase (<italic>SlPME</italic>
), β-galactosidase precursor mRNA (<italic>SlTBG</italic>
), xyloglucan endotransglycosylase (<italic>SlXET</italic>
), endo-1,4-β-cellulose (<italic>SlCels</italic>
), and expansin (<italic>SlExp</italic>
). This resulted in an increased accumulation of pectin during ripening. In turn, this led to a significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%. In conclusion, ABA affects cell wall catabolism during tomato fruit ripening via down-regulation of the expression of major catabolic genes (<italic>SlPG</italic>
, <italic>SlPME</italic>
, <italic>SlTBG</italic>
, <italic>SlXET</italic>
, <italic>SlCels</italic>
, and <italic>SlExp</italic>
).</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Pei" sort="Chen, Pei" uniqKey="Chen P" first="Pei" last="Chen">Pei Chen</name>
<name sortKey="Cui, Mengmeng" sort="Cui, Mengmeng" uniqKey="Cui M" first="Mengmeng" last="Cui">Mengmeng Cui</name>
<name sortKey="Dai, Shengjie" sort="Dai, Shengjie" uniqKey="Dai S" first="Shengjie" last="Dai">Shengjie Dai</name>
<name sortKey="Duan, Chaorui" sort="Duan, Chaorui" uniqKey="Duan C" first="Chaorui" last="Duan">Chaorui Duan</name>
<name sortKey="Ji, Kai" sort="Ji, Kai" uniqKey="Ji K" first="Kai" last="Ji">Kai Ji</name>
<name sortKey="Leng, Ping" sort="Leng, Ping" uniqKey="Leng P" first="Ping" last="Leng">Ping Leng</name>
<name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
<name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name>
<name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
<name sortKey="Sun, Liang" sort="Sun, Liang" uniqKey="Sun L" first="Liang" last="Sun">Liang Sun</name>
<name sortKey="Sun, Yufei" sort="Sun, Yufei" uniqKey="Sun Y" first="Yufei" last="Sun">Yufei Sun</name>
<name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name>
<name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name>
<name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name>
<name sortKey="Zhang, Mei" sort="Zhang, Mei" uniqKey="Zhang M" first="Mei" last="Zhang">Mei Zhang</name>
</noCountry>
</tree>
</affiliations>
</record>

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Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA]

Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA]

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

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Pour manipuler ce document sous Unix (Dilib)

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