Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA]
Identifieur interne : 000C19 ( Pmc/Checkpoint ); précédent : 000C18; suivant : 000C20Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA]
Auteurs : Liang Sun ; Yufei Sun ; Mei Zhang ; Ling Wang ; Jie Ren ; Mengmeng Cui ; Yanping Wang ; Kai Ji ; Ping Li ; Qian Li ; Pei Chen ; Shengjie Dai ; Chaorui Duan ; Yan Wu ; Ping LengSource :
- Plant Physiology [ 0032-0889 ] ; 2012.
Abstract
Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (
Url:
DOI: 10.1104/pp.111.186866
PubMed: 22108525
PubMed Central: 3252109
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:3252109Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Suppression of 9<italic>-</italic>
cis<italic>-</italic>
Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn3"><sup>[W]</sup>
</xref>
<xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup>
</xref>
</title>
<author><name sortKey="Sun, Liang" sort="Sun, Liang" uniqKey="Sun L" first="Liang" last="Sun">Liang Sun</name>
</author>
<author><name sortKey="Sun, Yufei" sort="Sun, Yufei" uniqKey="Sun Y" first="Yufei" last="Sun">Yufei Sun</name>
</author>
<author><name sortKey="Zhang, Mei" sort="Zhang, Mei" uniqKey="Zhang M" first="Mei" last="Zhang">Mei Zhang</name>
</author>
<author><name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name>
</author>
<author><name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
</author>
<author><name sortKey="Cui, Mengmeng" sort="Cui, Mengmeng" uniqKey="Cui M" first="Mengmeng" last="Cui">Mengmeng Cui</name>
</author>
<author><name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name>
</author>
<author><name sortKey="Ji, Kai" sort="Ji, Kai" uniqKey="Ji K" first="Kai" last="Ji">Kai Ji</name>
</author>
<author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
</author>
<author><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name>
</author>
<author><name sortKey="Chen, Pei" sort="Chen, Pei" uniqKey="Chen P" first="Pei" last="Chen">Pei Chen</name>
</author>
<author><name sortKey="Dai, Shengjie" sort="Dai, Shengjie" uniqKey="Dai S" first="Shengjie" last="Dai">Shengjie Dai</name>
</author>
<author><name sortKey="Duan, Chaorui" sort="Duan, Chaorui" uniqKey="Duan C" first="Chaorui" last="Duan">Chaorui Duan</name>
</author>
<author><name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name>
</author>
<author><name sortKey="Leng, Ping" sort="Leng, Ping" uniqKey="Leng P" first="Ping" last="Leng">Ping Leng</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22108525</idno>
<idno type="pmc">3252109</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252109</idno>
<idno type="RBID">PMC:3252109</idno>
<idno type="doi">10.1104/pp.111.186866</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">000C96</idno>
<idno type="wicri:Area/Pmc/Curation">000C95</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000C19</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Suppression of 9<italic>-</italic>
cis<italic>-</italic>
Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn3"><sup>[W]</sup>
</xref>
<xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup>
</xref>
</title>
<author><name sortKey="Sun, Liang" sort="Sun, Liang" uniqKey="Sun L" first="Liang" last="Sun">Liang Sun</name>
</author>
<author><name sortKey="Sun, Yufei" sort="Sun, Yufei" uniqKey="Sun Y" first="Yufei" last="Sun">Yufei Sun</name>
</author>
<author><name sortKey="Zhang, Mei" sort="Zhang, Mei" uniqKey="Zhang M" first="Mei" last="Zhang">Mei Zhang</name>
</author>
<author><name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name>
</author>
<author><name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
</author>
<author><name sortKey="Cui, Mengmeng" sort="Cui, Mengmeng" uniqKey="Cui M" first="Mengmeng" last="Cui">Mengmeng Cui</name>
</author>
<author><name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name>
</author>
<author><name sortKey="Ji, Kai" sort="Ji, Kai" uniqKey="Ji K" first="Kai" last="Ji">Kai Ji</name>
</author>
<author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
</author>
<author><name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name>
</author>
<author><name sortKey="Chen, Pei" sort="Chen, Pei" uniqKey="Chen P" first="Pei" last="Chen">Pei Chen</name>
</author>
<author><name sortKey="Dai, Shengjie" sort="Dai, Shengjie" uniqKey="Dai S" first="Shengjie" last="Dai">Shengjie Dai</name>
</author>
<author><name sortKey="Duan, Chaorui" sort="Duan, Chaorui" uniqKey="Duan C" first="Chaorui" last="Duan">Chaorui Duan</name>
</author>
<author><name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name>
</author>
<author><name sortKey="Leng, Ping" sort="Leng, Ping" uniqKey="Leng P" first="Ping" last="Leng">Ping Leng</name>
</author>
</analytic>
<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
<idno type="eISSN">1532-2548</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (<italic>Solanum lycopersicum</italic>
) fruit ripening, we suppressed <italic>SlNCED1</italic>
, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of ABA. To suppress <italic>SlNCED1</italic>
specifically in tomato fruits, and thus avoid the pleiotropic phenotypes associated with ABA deficiency, we used an RNA interference construct driven by the fruit-specific <italic>E8</italic>
promoter. ABA accumulation and <italic>SlNCED1</italic>
transcript levels in the transgenic fruit were down-regulated to between 20% and 50% of the levels measured in the control fruit. This significant reduction in NCED activity led to a down-regulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase (<italic>SlPG</italic>
), pectin methyl esterase (<italic>SlPME</italic>
), β-galactosidase precursor mRNA (<italic>SlTBG</italic>
), xyloglucan endotransglycosylase (<italic>SlXET</italic>
), endo-1,4-β-cellulose (<italic>SlCels</italic>
), and expansin (<italic>SlExp</italic>
). This resulted in an increased accumulation of pectin during ripening. In turn, this led to a significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%. In conclusion, ABA affects cell wall catabolism during tomato fruit ripening via down-regulation of the expression of major catabolic genes (<italic>SlPG</italic>
, <italic>SlPME</italic>
, <italic>SlTBG</italic>
, <italic>SlXET</italic>
, <italic>SlCels</italic>
, and <italic>SlExp</italic>
).</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id>
<journal-id journal-id-type="hwp">plantphysiol</journal-id>
<journal-id journal-id-type="publisher-id">aspb</journal-id>
<journal-title-group><journal-title>Plant Physiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0032-0889</issn>
<issn pub-type="epub">1532-2548</issn>
<publisher><publisher-name>American Society of Plant Biologists</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22108525</article-id>
<article-id pub-id-type="pmc">3252109</article-id>
<article-id pub-id-type="publisher-id">186866</article-id>
<article-id pub-id-type="doi">10.1104/pp.111.186866</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Development and Hormone Action</subject>
</subj-group>
</article-categories>
<title-group><article-title>Suppression of 9<italic>-</italic>
cis<italic>-</italic>
Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
<xref ref-type="author-notes" rid="fn3"><sup>[W]</sup>
</xref>
<xref ref-type="author-notes" rid="fn4"><sup>[OA]</sup>
</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Sun</surname>
<given-names>Liang</given-names>
</name>
<xref ref-type="author-notes" rid="fn2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sun</surname>
<given-names>Yufei</given-names>
</name>
<xref ref-type="author-notes" rid="fn2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Mei</given-names>
</name>
<xref ref-type="author-notes" rid="fn2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Ling</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Ren</surname>
<given-names>Jie</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Cui</surname>
<given-names>Mengmeng</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Yanping</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Ji</surname>
<given-names>Kai</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Ping</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Qian</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Pei</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Dai</surname>
<given-names>Shengjie</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Duan</surname>
<given-names>Chaorui</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Wu</surname>
<given-names>Yan</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Leng</surname>
<given-names>Ping</given-names>
</name>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff>College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China (L.S., Y.S., L.W., J.R., M.C., Y.W., K.J., P.Li, Q.L., P.C., S.D., C.D., Y.W., P.Le.); and Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China (M.Z.)</aff>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author; e-mail <email>pleng@cau.edu.cn</email>
</corresp>
<fn id="fn1"><label>1</label>
<p>This work was supported by the Beijing Natural Science Foundation (grant no. 6052013) and the Beijing Municipal Science and Technology Commission (grant no. D0706002000091).</p>
</fn>
<fn id="fn2" fn-type="equal"><label>2</label>
<p>These authors contributed equally to the article.</p>
</fn>
<fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org">www.plantphysiol.org</ext-link>
) is: Ping Leng (<email>pleng@cau.edu.cn</email>
).</p>
</fn>
<fn id="fn3"><label>[W]</label>
<p>The online version of this article contains Web-only data.</p>
</fn>
<fn id="fn4"><label>[OA]</label>
<p>Open Access articles can be viewed online without a subscription.</p>
</fn>
<fn><p><ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org/cgi/doi/10.1104/pp.111.186866">www.plantphysiol.org/cgi/doi/10.1104/pp.111.186866</ext-link>
</p>
</fn>
</author-notes>
<pub-date pub-type="epub-ppub"><month>1</month>
<year>2012</year>
</pub-date>
<pmc-comment>Fake ppub date generated by PMC from publisher pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><month>1</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="pmc-release"><month>1</month>
<year>2012</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>158</volume>
<issue>1</issue>
<fpage>283</fpage>
<lpage>298</lpage>
<history><date date-type="received"><day>10</day>
<month>9</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>19</day>
<month>11</month>
<year>2011</year>
</date>
</history>
<permissions><copyright-statement>© 2012 American Society of Plant Biologists. All rights reserved.</copyright-statement>
<copyright-year>2012</copyright-year>
</permissions>
<abstract><p>Cell wall catabolism during fruit ripening is under complex control and is key for fruit quality and shelf life. To examine the role of abscisic acid (ABA) in tomato (<italic>Solanum lycopersicum</italic>
) fruit ripening, we suppressed <italic>SlNCED1</italic>
, which encodes 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of ABA. To suppress <italic>SlNCED1</italic>
specifically in tomato fruits, and thus avoid the pleiotropic phenotypes associated with ABA deficiency, we used an RNA interference construct driven by the fruit-specific <italic>E8</italic>
promoter. ABA accumulation and <italic>SlNCED1</italic>
transcript levels in the transgenic fruit were down-regulated to between 20% and 50% of the levels measured in the control fruit. This significant reduction in NCED activity led to a down-regulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase (<italic>SlPG</italic>
), pectin methyl esterase (<italic>SlPME</italic>
), β-galactosidase precursor mRNA (<italic>SlTBG</italic>
), xyloglucan endotransglycosylase (<italic>SlXET</italic>
), endo-1,4-β-cellulose (<italic>SlCels</italic>
), and expansin (<italic>SlExp</italic>
). This resulted in an increased accumulation of pectin during ripening. In turn, this led to a significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%. In conclusion, ABA affects cell wall catabolism during tomato fruit ripening via down-regulation of the expression of major catabolic genes (<italic>SlPG</italic>
, <italic>SlPME</italic>
, <italic>SlTBG</italic>
, <italic>SlXET</italic>
, <italic>SlCels</italic>
, and <italic>SlExp</italic>
).</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Pei" sort="Chen, Pei" uniqKey="Chen P" first="Pei" last="Chen">Pei Chen</name>
<name sortKey="Cui, Mengmeng" sort="Cui, Mengmeng" uniqKey="Cui M" first="Mengmeng" last="Cui">Mengmeng Cui</name>
<name sortKey="Dai, Shengjie" sort="Dai, Shengjie" uniqKey="Dai S" first="Shengjie" last="Dai">Shengjie Dai</name>
<name sortKey="Duan, Chaorui" sort="Duan, Chaorui" uniqKey="Duan C" first="Chaorui" last="Duan">Chaorui Duan</name>
<name sortKey="Ji, Kai" sort="Ji, Kai" uniqKey="Ji K" first="Kai" last="Ji">Kai Ji</name>
<name sortKey="Leng, Ping" sort="Leng, Ping" uniqKey="Leng P" first="Ping" last="Leng">Ping Leng</name>
<name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name>
<name sortKey="Li, Qian" sort="Li, Qian" uniqKey="Li Q" first="Qian" last="Li">Qian Li</name>
<name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
<name sortKey="Sun, Liang" sort="Sun, Liang" uniqKey="Sun L" first="Liang" last="Sun">Liang Sun</name>
<name sortKey="Sun, Yufei" sort="Sun, Yufei" uniqKey="Sun Y" first="Yufei" last="Sun">Yufei Sun</name>
<name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name>
<name sortKey="Wang, Yanping" sort="Wang, Yanping" uniqKey="Wang Y" first="Yanping" last="Wang">Yanping Wang</name>
<name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name>
<name sortKey="Zhang, Mei" sort="Zhang, Mei" uniqKey="Zhang M" first="Mei" last="Zhang">Mei Zhang</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C19 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000C19 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Pmc |étape= Checkpoint |type= RBID |clé= PMC:3252109 |texte= Suppression of 9-cis-Epoxycarotenoid Dioxygenase, Which Encodes a Key Enzyme in Abscisic Acid Biosynthesis, Alters Fruit Texture in Transgenic Tomato1[W][OA] }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:22108525" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
This area was generated with Dilib version V0.6.25. |