Structural Analysis and Involvement in Plant Innate Immunity of Xanthomonas axonopodis pv. citri Lipopolysaccharide*
Identifieur interne : 000D39 ( Ncbi/Checkpoint ); précédent : 000D38; suivant : 000D40Structural Analysis and Involvement in Plant Innate Immunity of Xanthomonas axonopodis pv. citri Lipopolysaccharide*
Auteurs : Adriana Casabuono ; Silvana Petrocelli ; Jorgelina Ottado ; Elena G. Orellano ; Alicia S. CoutoSource :
- The Journal of Biological Chemistry [ 0021-9258 ] ; 2011.
English descriptors
- KwdEn :
- ATP-Binding Cassette Transporters (chemistry), ATP-Binding Cassette Transporters (genetics), Bacterial Proteins (chemistry), Bacterial Proteins (genetics), Carbohydrate Sequence, Citrus sinensis (anatomy & histology), Citrus sinensis (genetics), Citrus sinensis (immunology), Citrus sinensis (microbiology), Gene Expression Regulation, Plant (immunology), Host-Pathogen Interactions, Immunity, Innate (genetics), Lipopolysaccharides (biosynthesis), Lipopolysaccharides (chemistry), Lipopolysaccharides (isolation & purification), Lipopolysaccharides (metabolism), Molecular Sequence Data, Peroxides (metabolism), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (microbiology), Plant Stomata (anatomy & histology), Plant Stomata (immunology), Plant Stomata (microbiology), Xanthomonas axonopodis (metabolism), Xanthomonas axonopodis (physiology).
- MESH :
- chemical , biosynthesis : Lipopolysaccharides.
- chemical , chemistry : Bacterial Proteins, Lipopolysaccharides.
- anatomy & histology : Citrus sinensis, Plant Stomata.
- chemistry : ATP-Binding Cassette Transporters.
- genetics : ATP-Binding Cassette Transporters, Bacterial Proteins, Citrus sinensis, Immunity, Innate, Plant Leaves.
- immunology : Citrus sinensis, Gene Expression Regulation, Plant, Plant Leaves, Plant Stomata.
- chemical , isolation & purification : Lipopolysaccharides.
- chemical , metabolism : Lipopolysaccharides, Peroxides, Xanthomonas axonopodis.
- microbiology : Citrus sinensis, Plant Leaves, Plant Stomata.
- physiology : Xanthomonas axonopodis.
- Carbohydrate Sequence, Host-Pathogen Interactions, Molecular Sequence Data.
Abstract
Url:
DOI: 10.1074/jbc.M110.186049
PubMed: 21596742
PubMed Central: 3138316
Affiliations:
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PMC:3138316Le document en format XML
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pv. <italic>citri</italic>
Lipopolysaccharide<xref ref-type="fn" rid="FN1">*</xref>
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<author><name sortKey="Ottado, Jorgelina" sort="Ottado, Jorgelina" uniqKey="Ottado J" first="Jorgelina" last="Ottado">Jorgelina Ottado</name>
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pv. <italic>citri</italic>
Lipopolysaccharide<xref ref-type="fn" rid="FN1">*</xref>
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<author><name sortKey="Petrocelli, Silvana" sort="Petrocelli, Silvana" uniqKey="Petrocelli S" first="Silvana" last="Petrocelli">Silvana Petrocelli</name>
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<author><name sortKey="Ottado, Jorgelina" sort="Ottado, Jorgelina" uniqKey="Ottado J" first="Jorgelina" last="Ottado">Jorgelina Ottado</name>
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<author><name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. Orellano</name>
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<author><name sortKey="Couto, Alicia S" sort="Couto, Alicia S" uniqKey="Couto A" first="Alicia S." last="Couto">Alicia S. Couto</name>
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<series><title level="j">The Journal of Biological Chemistry</title>
<idno type="ISSN">0021-9258</idno>
<idno type="eISSN">1083-351X</idno>
<imprint><date when="2011">2011</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ATP-Binding Cassette Transporters (chemistry)</term>
<term>ATP-Binding Cassette Transporters (genetics)</term>
<term>Bacterial Proteins (chemistry)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Carbohydrate Sequence</term>
<term>Citrus sinensis (anatomy & histology)</term>
<term>Citrus sinensis (genetics)</term>
<term>Citrus sinensis (immunology)</term>
<term>Citrus sinensis (microbiology)</term>
<term>Gene Expression Regulation, Plant (immunology)</term>
<term>Host-Pathogen Interactions</term>
<term>Immunity, Innate (genetics)</term>
<term>Lipopolysaccharides (biosynthesis)</term>
<term>Lipopolysaccharides (chemistry)</term>
<term>Lipopolysaccharides (isolation & purification)</term>
<term>Lipopolysaccharides (metabolism)</term>
<term>Molecular Sequence Data</term>
<term>Peroxides (metabolism)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (immunology)</term>
<term>Plant Leaves (microbiology)</term>
<term>Plant Stomata (anatomy & histology)</term>
<term>Plant Stomata (immunology)</term>
<term>Plant Stomata (microbiology)</term>
<term>Xanthomonas axonopodis (metabolism)</term>
<term>Xanthomonas axonopodis (physiology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lipopolysaccharides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Bacterial Proteins</term>
<term>Lipopolysaccharides</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Citrus sinensis</term>
<term>Plant Stomata</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>ATP-Binding Cassette Transporters</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>ATP-Binding Cassette Transporters</term>
<term>Bacterial Proteins</term>
<term>Citrus sinensis</term>
<term>Immunity, Innate</term>
<term>Plant Leaves</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Citrus sinensis</term>
<term>Gene Expression Regulation, Plant</term>
<term>Plant Leaves</term>
<term>Plant Stomata</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Lipopolysaccharides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lipopolysaccharides</term>
<term>Peroxides</term>
<term>Xanthomonas axonopodis</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term>
<term>Plant Leaves</term>
<term>Plant Stomata</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Xanthomonas axonopodis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Sequence</term>
<term>Host-Pathogen Interactions</term>
<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en"><p><italic>Xanthomonas axonopodis</italic>
pv. <italic>citri</italic>
(Xac) causes citrus canker, provoking defoliation and premature fruit drop with concomitant economical damage. In plant pathogenic bacteria, lipopolysaccharides are important virulence factors, and they are being increasingly recognized as major pathogen-associated molecular patterns for plants. In general, three domains are recognized in a lipopolysaccharide: the hydrophobic lipid A, the hydrophilic O-antigen polysaccharide, and the core oligosaccharide, connecting lipid A and O-antigen. In this work, we have determined the structure of purified lipopolysaccharides obtained from <italic>Xanthomonas axonopodis</italic>
pv. <italic>citri</italic>
wild type and a mutant of the O-antigen ABC transporter encoded by the <italic>wzt</italic>
gene. High pH anion exchange chromatography and matrix-assisted laser desorption/ionization mass spectrum analysis were performed, enabling determination of the structure not only of the released oligosaccharides and lipid A moieties but also the intact lipopolysaccharides. The results demonstrate that Xac wild type and Xac<italic>wzt</italic>
LPSs are composed mainly of a penta- or tetra-acylated diglucosamine backbone attached to either two pyrophosphorylethanolamine groups or to one pyrophosphorylethanolamine group and one phosphorylethanolamine group. The core region consists of a branched oligosaccharide formed by Kdo<sub>2</sub>
Hex<sub>6</sub>
GalA<sub>3</sub>
Fuc3NAcRha<sub>4</sub>
and two phosphate groups. As expected, the presence of a rhamnose homo-oligosaccharide as O-antigen was determined only in the Xac wild type lipopolysaccharide. In addition, we have examined how lipopolysaccharides from Xac function in the pathogenesis process. We analyzed the response of the different lipopolysaccharides during the stomata aperture closure cycle, the callose deposition, the expression of defense-related genes, and reactive oxygen species production in citrus leaves, suggesting a functional role of the O-antigen from Xac lipopolysaccharides in the basal response.</p>
</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Casabuono, Adriana" sort="Casabuono, Adriana" uniqKey="Casabuono A" first="Adriana" last="Casabuono">Adriana Casabuono</name>
<name sortKey="Couto, Alicia S" sort="Couto, Alicia S" uniqKey="Couto A" first="Alicia S." last="Couto">Alicia S. Couto</name>
<name sortKey="Orellano, Elena G" sort="Orellano, Elena G" uniqKey="Orellano E" first="Elena G." last="Orellano">Elena G. Orellano</name>
<name sortKey="Ottado, Jorgelina" sort="Ottado, Jorgelina" uniqKey="Ottado J" first="Jorgelina" last="Ottado">Jorgelina Ottado</name>
<name sortKey="Petrocelli, Silvana" sort="Petrocelli, Silvana" uniqKey="Petrocelli S" first="Silvana" last="Petrocelli">Silvana Petrocelli</name>
</noCountry>
</tree>
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