Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.
Identifieur interne : 000A65 ( Ncbi/Checkpoint ); précédent : 000A64; suivant : 000A66Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.
Auteurs : Shuqing Dong [République populaire de Chine] ; Langzhu Chi ; Pingang He ; Qingjiang Wang ; Yuzhi FangSource :
- Talanta [ 1873-3573 ] ; 2009.
English descriptors
- KwdEn :
- Antioxidants (analysis), Antioxidants (chemistry), Ascorbic Acid (analysis), Ascorbic Acid (chemistry), Caffeic Acids (analysis), Caffeic Acids (chemistry), Chlorogenic Acid (analysis), Chlorogenic Acid (chemistry), Citrus sinensis (chemistry), Electrochemistry (instrumentation), Electrochemistry (methods), Electrodes, Electrophoresis, Capillary (instrumentation), Electrophoresis, Capillary (methods), Glutathione (analysis), Glutathione (chemistry), Hydrogen-Ion Concentration, Lycopersicon esculentum (chemistry), Oxidation-Reduction, Reproducibility of Results, Salicylates (analysis), Salicylates (chemistry), Vanillic Acid (analysis), Vanillic Acid (chemistry), Vitamin B 12 (chemistry).
- MESH :
- chemical , analysis : Antioxidants, Ascorbic Acid, Caffeic Acids, Chlorogenic Acid, Glutathione, Salicylates, Vanillic Acid.
- chemical , chemistry : Antioxidants, Ascorbic Acid, Caffeic Acids, Chlorogenic Acid, Glutathione, Salicylates, Vanillic Acid, Vitamin B 12.
- chemistry : Citrus sinensis, Lycopersicon esculentum.
- instrumentation : Electrochemistry, Electrophoresis, Capillary.
- methods : Electrochemistry, Electrophoresis, Capillary.
- Electrodes, Hydrogen-Ion Concentration, Oxidation-Reduction, Reproducibility of Results.
Abstract
In the paper, a new kind of vitamin B(12) (acquo-cobalamine) chemically modified electrode was fabricated and applied in capillary zone electrophoresis coupled with amperometric detection (CZE-AD) for simultaneous determination of six antioxidants in fruits and vegetables. The catalytic electrochemical properties of the chemically modified electrode could obviously enhance oxidation peak heights responses by about five times to glutathione, ascorbic acid, vanillic acid, chlorogenic acid, salicylic acid, and caffeic acid compared with common carbon disk electrode. Furthermore, the effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CZE-AD were investigated. Under the optimum conditions, the six analytes could be completely separated and detected in a borate-phosphate buffer (pH 8.4) within 15 min. Their linear ranges were from 2.5x10(-7) to 1.0x10(-4) mol L(-1) and the detection limits were as low as 10(-8) mol L(-1) magnitude (S/N=3). The proposed method has been successfully employed to monitor the six analytes in practical samples with recoveries in the range 96.0-106.0% and RSDs less than 5.0%. Above results demonstrate that capillary zone electrophoresis coupled with electrochemical detection using vitamin B(12) modified electrode as detector is of convenient preparation, high sensitivity, good repeatability, and could be used in the rapid determination of practical samples.
DOI: 10.1016/j.talanta.2009.07.069
PubMed: 19836556
Affiliations:
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<author><name sortKey="Dong, Shuqing" sort="Dong, Shuqing" uniqKey="Dong S" first="Shuqing" last="Dong">Shuqing Dong</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, East China Normal University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Chemistry, East China Normal University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
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<author><name sortKey="Chi, Langzhu" sort="Chi, Langzhu" uniqKey="Chi L" first="Langzhu" last="Chi">Langzhu Chi</name>
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<author><name sortKey="He, Pingang" sort="He, Pingang" uniqKey="He P" first="Pingang" last="He">Pingang He</name>
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<author><name sortKey="Wang, Qingjiang" sort="Wang, Qingjiang" uniqKey="Wang Q" first="Qingjiang" last="Wang">Qingjiang Wang</name>
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<author><name sortKey="Fang, Yuzhi" sort="Fang, Yuzhi" uniqKey="Fang Y" first="Yuzhi" last="Fang">Yuzhi Fang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.</title>
<author><name sortKey="Dong, Shuqing" sort="Dong, Shuqing" uniqKey="Dong S" first="Shuqing" last="Dong">Shuqing Dong</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, East China Normal University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Chemistry, East China Normal University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
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<author><name sortKey="Chi, Langzhu" sort="Chi, Langzhu" uniqKey="Chi L" first="Langzhu" last="Chi">Langzhu Chi</name>
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<author><name sortKey="He, Pingang" sort="He, Pingang" uniqKey="He P" first="Pingang" last="He">Pingang He</name>
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<author><name sortKey="Wang, Qingjiang" sort="Wang, Qingjiang" uniqKey="Wang Q" first="Qingjiang" last="Wang">Qingjiang Wang</name>
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<author><name sortKey="Fang, Yuzhi" sort="Fang, Yuzhi" uniqKey="Fang Y" first="Yuzhi" last="Fang">Yuzhi Fang</name>
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<series><title level="j">Talanta</title>
<idno type="eISSN">1873-3573</idno>
<imprint><date when="2009" type="published">2009</date>
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<term>Antioxidants (chemistry)</term>
<term>Ascorbic Acid (analysis)</term>
<term>Ascorbic Acid (chemistry)</term>
<term>Caffeic Acids (analysis)</term>
<term>Caffeic Acids (chemistry)</term>
<term>Chlorogenic Acid (analysis)</term>
<term>Chlorogenic Acid (chemistry)</term>
<term>Citrus sinensis (chemistry)</term>
<term>Electrochemistry (instrumentation)</term>
<term>Electrochemistry (methods)</term>
<term>Electrodes</term>
<term>Electrophoresis, Capillary (instrumentation)</term>
<term>Electrophoresis, Capillary (methods)</term>
<term>Glutathione (analysis)</term>
<term>Glutathione (chemistry)</term>
<term>Hydrogen-Ion Concentration</term>
<term>Lycopersicon esculentum (chemistry)</term>
<term>Oxidation-Reduction</term>
<term>Reproducibility of Results</term>
<term>Salicylates (analysis)</term>
<term>Salicylates (chemistry)</term>
<term>Vanillic Acid (analysis)</term>
<term>Vanillic Acid (chemistry)</term>
<term>Vitamin B 12 (chemistry)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antioxidants</term>
<term>Ascorbic Acid</term>
<term>Caffeic Acids</term>
<term>Chlorogenic Acid</term>
<term>Glutathione</term>
<term>Salicylates</term>
<term>Vanillic Acid</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antioxidants</term>
<term>Ascorbic Acid</term>
<term>Caffeic Acids</term>
<term>Chlorogenic Acid</term>
<term>Glutathione</term>
<term>Salicylates</term>
<term>Vanillic Acid</term>
<term>Vitamin B 12</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term>
<term>Lycopersicon esculentum</term>
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<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrochemistry</term>
<term>Electrophoresis, Capillary</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrochemistry</term>
<term>Electrophoresis, Capillary</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Electrodes</term>
<term>Hydrogen-Ion Concentration</term>
<term>Oxidation-Reduction</term>
<term>Reproducibility of Results</term>
</keywords>
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<front><div type="abstract" xml:lang="en">In the paper, a new kind of vitamin B(12) (acquo-cobalamine) chemically modified electrode was fabricated and applied in capillary zone electrophoresis coupled with amperometric detection (CZE-AD) for simultaneous determination of six antioxidants in fruits and vegetables. The catalytic electrochemical properties of the chemically modified electrode could obviously enhance oxidation peak heights responses by about five times to glutathione, ascorbic acid, vanillic acid, chlorogenic acid, salicylic acid, and caffeic acid compared with common carbon disk electrode. Furthermore, the effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CZE-AD were investigated. Under the optimum conditions, the six analytes could be completely separated and detected in a borate-phosphate buffer (pH 8.4) within 15 min. Their linear ranges were from 2.5x10(-7) to 1.0x10(-4) mol L(-1) and the detection limits were as low as 10(-8) mol L(-1) magnitude (S/N=3). The proposed method has been successfully employed to monitor the six analytes in practical samples with recoveries in the range 96.0-106.0% and RSDs less than 5.0%. Above results demonstrate that capillary zone electrophoresis coupled with electrochemical detection using vitamin B(12) modified electrode as detector is of convenient preparation, high sensitivity, good repeatability, and could be used in the rapid determination of practical samples.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
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<tree><noCountry><name sortKey="Chi, Langzhu" sort="Chi, Langzhu" uniqKey="Chi L" first="Langzhu" last="Chi">Langzhu Chi</name>
<name sortKey="Fang, Yuzhi" sort="Fang, Yuzhi" uniqKey="Fang Y" first="Yuzhi" last="Fang">Yuzhi Fang</name>
<name sortKey="He, Pingang" sort="He, Pingang" uniqKey="He P" first="Pingang" last="He">Pingang He</name>
<name sortKey="Wang, Qingjiang" sort="Wang, Qingjiang" uniqKey="Wang Q" first="Qingjiang" last="Wang">Qingjiang Wang</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Dong, Shuqing" sort="Dong, Shuqing" uniqKey="Dong S" first="Shuqing" last="Dong">Shuqing Dong</name>
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