The association of homeobox gene expression with stem cell formation and morphogenesis in cultured Medicago truncatula
Identifieur interne : 000A31 ( Ncbi/Checkpoint ); précédent : 000A30; suivant : 000A32The association of homeobox gene expression with stem cell formation and morphogenesis in cultured Medicago truncatula
Auteurs : S.-K. Chen ; S. Kurdyukov ; A. Kereszt [Hongrie] ; X.-D. Wang ; P. M. Gresshoff ; R. J. RoseSource :
- Planta [ 0032-0935 ] ; 2009.
Abstract
Somatic embryogenesis (SE) is induced in vitro in
The online version of this article (doi:10.1007/s00425-009-0988-1) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s00425-009-0988-1
PubMed: 19639337
PubMed Central: 2729979
Affiliations:
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<author><name sortKey="Chen, S K" sort="Chen, S K" uniqKey="Chen S" first="S.-K." last="Chen">S.-K. Chen</name>
<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
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<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
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<affiliation wicri:level="1"><nlm:aff id="Aff3">Baygen Institute, 6726 Szeged, Hungary</nlm:aff>
<country xml:lang="fr">Hongrie</country>
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<author><name sortKey="Wang, X D" sort="Wang, X D" uniqKey="Wang X" first="X.-D." last="Wang">X.-D. Wang</name>
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<author><name sortKey="Chen, S K" sort="Chen, S K" uniqKey="Chen S" first="S.-K." last="Chen">S.-K. Chen</name>
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<author><name sortKey="Kurdyukov, S" sort="Kurdyukov, S" uniqKey="Kurdyukov S" first="S." last="Kurdyukov">S. Kurdyukov</name>
<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
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<author><name sortKey="Kereszt, A" sort="Kereszt, A" uniqKey="Kereszt A" first="A." last="Kereszt">A. Kereszt</name>
<affiliation><nlm:aff id="Aff2">Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Queensland, Brisbane, QLD 4072 Australia</nlm:aff>
<wicri:noCountry code="subfield">QLD 4072 Australia</wicri:noCountry>
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<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
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<author><name sortKey="Gresshoff, P M" sort="Gresshoff, P M" uniqKey="Gresshoff P" first="P. M." last="Gresshoff">P. M. Gresshoff</name>
<affiliation><nlm:aff id="Aff2">Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Queensland, Brisbane, QLD 4072 Australia</nlm:aff>
<wicri:noCountry code="subfield">QLD 4072 Australia</wicri:noCountry>
</affiliation>
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<author><name sortKey="Rose, R J" sort="Rose, R J" uniqKey="Rose R" first="R. J." last="Rose">R. J. Rose</name>
<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
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<series><title level="j">Planta</title>
<idno type="ISSN">0032-0935</idno>
<idno type="eISSN">1432-2048</idno>
<imprint><date when="2009">2009</date>
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<front><div type="abstract" xml:lang="en"><p>Somatic embryogenesis (SE) is induced in vitro in <italic>Medicago truncatula</italic>
2HA by auxin and cytokinin but rarely in wild type Jemalong. The putative <italic>WUSCHEL</italic>
(<italic>MtWUS</italic>
), <italic>CLAVATA3</italic>
(<italic>MtCLV3</italic>
) and the <italic>WUSCHEL</italic>
-related homeobox gene <italic>WOX5</italic>
(<italic>MtWOX5)</italic>
were investigated in <italic>M. truncatula</italic>
(<italic>Mt</italic>
) and identified by the similarity to <italic>Arabidopsis WUS, CLV3</italic>
and <italic>WOX5</italic>
in amino acid sequence, phylogeny and in planta and in vitro expression patterns. <italic>MtWUS</italic>
was induced throughout embryogenic cultures by cytokinin after 24–48 h and maximum expression occurred after 1 week, which coincides with the induction of totipotent stem cells. During this period there was no <italic>MtCLV3</italic>
expression to suppress <italic>MtWUS. MtWUS</italic>
expression, as illustrated by promoter-GUS studies, subsequently localised to the embryo, and there was then the onset of <italic>MtCLV3</italic>
expression. This suggests that the expression of the putative <italic>MtCLV3</italic>
coincides with the WUS-CLAVATA feedback loop becoming operational. RNAi studies showed that <italic>MtWUS</italic>
expression is essential for callus and somatic embryo production. Based on the presence of <italic>MtWUS</italic>
promoter binding sites, <italic>MtWUS</italic>
may be required for the induction of <italic>MtSERF1,</italic>
postulated to have a key role in the signalling required for SE induced in 2HA. <italic>MtWOX5</italic>
expressed in auxin-induced root primordia and root meristems and appears to be involved in pluripotent stem cell induction. The evidence is discussed that the homeobox genes <italic>MtWUS</italic>
and <italic>MtWOX5</italic>
are “hijacked” for stem cell induction, which is key to somatic embryo and de novo root induction. In relation to SE, a role for <italic>WUS</italic>
in the signalling involved in induction is discussed.</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1007/s00425-009-0988-1) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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<affiliations><list><country><li>Hongrie</li>
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<tree><noCountry><name sortKey="Chen, S K" sort="Chen, S K" uniqKey="Chen S" first="S.-K." last="Chen">S.-K. Chen</name>
<name sortKey="Gresshoff, P M" sort="Gresshoff, P M" uniqKey="Gresshoff P" first="P. M." last="Gresshoff">P. M. Gresshoff</name>
<name sortKey="Kurdyukov, S" sort="Kurdyukov, S" uniqKey="Kurdyukov S" first="S." last="Kurdyukov">S. Kurdyukov</name>
<name sortKey="Rose, R J" sort="Rose, R J" uniqKey="Rose R" first="R. J." last="Rose">R. J. Rose</name>
<name sortKey="Wang, X D" sort="Wang, X D" uniqKey="Wang X" first="X.-D." last="Wang">X.-D. Wang</name>
</noCountry>
<country name="Hongrie"><noRegion><name sortKey="Kereszt, A" sort="Kereszt, A" uniqKey="Kereszt A" first="A." last="Kereszt">A. Kereszt</name>
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