The association of homeobox gene expression with stem cell formation and morphogenesis in cultured Medicago truncatula
Identifieur interne : 000995 ( Pmc/Curation ); précédent : 000994; suivant : 000996The association of homeobox gene expression with stem cell formation and morphogenesis in cultured Medicago truncatula
Auteurs : S.-K. Chen ; S. Kurdyukov ; A. Kereszt [Hongrie] ; X.-D. Wang ; P. M. Gresshoff ; R. J. RoseSource :
- Planta [ 0032-0935 ] ; 2009.
Abstract
Somatic embryogenesis (SE) is induced in vitro in
The online version of this article (doi:10.1007/s00425-009-0988-1) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s00425-009-0988-1
PubMed: 19639337
PubMed Central: 2729979
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S.-K. Chen<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff2">Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Queensland, Brisbane, QLD 4072 Australia</nlm:aff>
<wicri:noCountry code="subfield">QLD 4072 Australia</wicri:noCountry>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff3">Baygen Institute, 6726 Szeged, Hungary</nlm:aff>
<country xml:lang="fr">Hongrie</country>
<wicri:regionArea>Baygen Institute, 6726 Szeged</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff2">Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Queensland, Brisbane, QLD 4072 Australia</nlm:aff>
<wicri:noCountry code="subfield">QLD 4072 Australia</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff1">School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</nlm:aff>
<wicri:noCountry code="subfield">NSW 2308 Australia</wicri:noCountry>
</affiliation>
Le document en format XML
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<series><title level="j">Planta</title>
<idno type="ISSN">0032-0935</idno>
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<front><div type="abstract" xml:lang="en"><p>Somatic embryogenesis (SE) is induced in vitro in <italic>Medicago truncatula</italic>
2HA by auxin and cytokinin but rarely in wild type Jemalong. The putative <italic>WUSCHEL</italic>
(<italic>MtWUS</italic>
), <italic>CLAVATA3</italic>
(<italic>MtCLV3</italic>
) and the <italic>WUSCHEL</italic>
-related homeobox gene <italic>WOX5</italic>
(<italic>MtWOX5)</italic>
were investigated in <italic>M. truncatula</italic>
(<italic>Mt</italic>
) and identified by the similarity to <italic>Arabidopsis WUS, CLV3</italic>
and <italic>WOX5</italic>
in amino acid sequence, phylogeny and in planta and in vitro expression patterns. <italic>MtWUS</italic>
was induced throughout embryogenic cultures by cytokinin after 24–48 h and maximum expression occurred after 1 week, which coincides with the induction of totipotent stem cells. During this period there was no <italic>MtCLV3</italic>
expression to suppress <italic>MtWUS. MtWUS</italic>
expression, as illustrated by promoter-GUS studies, subsequently localised to the embryo, and there was then the onset of <italic>MtCLV3</italic>
expression. This suggests that the expression of the putative <italic>MtCLV3</italic>
coincides with the WUS-CLAVATA feedback loop becoming operational. RNAi studies showed that <italic>MtWUS</italic>
expression is essential for callus and somatic embryo production. Based on the presence of <italic>MtWUS</italic>
promoter binding sites, <italic>MtWUS</italic>
may be required for the induction of <italic>MtSERF1,</italic>
postulated to have a key role in the signalling required for SE induced in 2HA. <italic>MtWOX5</italic>
expressed in auxin-induced root primordia and root meristems and appears to be involved in pluripotent stem cell induction. The evidence is discussed that the homeobox genes <italic>MtWUS</italic>
and <italic>MtWOX5</italic>
are “hijacked” for stem cell induction, which is key to somatic embryo and de novo root induction. In relation to SE, a role for <italic>WUS</italic>
in the signalling involved in induction is discussed.</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1007/s00425-009-0988-1) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Planta</journal-id>
<journal-title>Planta</journal-title>
<issn pub-type="ppub">0032-0935</issn>
<issn pub-type="epub">1432-2048</issn>
<publisher><publisher-name>Springer-Verlag</publisher-name>
<publisher-loc>Berlin/Heidelberg</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19639337</article-id>
<article-id pub-id-type="pmc">2729979</article-id>
<article-id pub-id-type="publisher-id">988</article-id>
<article-id pub-id-type="doi">10.1007/s00425-009-0988-1</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>The association of homeobox gene expression with stem cell formation and morphogenesis in cultured <italic>Medicago truncatula</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name name-style="western"><surname>Chen</surname>
<given-names>S.-K.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name name-style="western"><surname>Kurdyukov</surname>
<given-names>S.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name name-style="western"><surname>Kereszt</surname>
<given-names>A.</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<contrib contrib-type="author"><name name-style="western"><surname>Wang</surname>
<given-names>X.-D.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name name-style="western"><surname>Gresshoff</surname>
<given-names>P. M.</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name name-style="western"><surname>Rose</surname>
<given-names>R. J.</given-names>
</name>
<address><phone>+61-2-49215711</phone>
<fax>+61-2-49215472</fax>
<email>Ray.Rose@newcastle.edu.au</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<aff id="Aff1"><label>1</label>
School of Environmental and Life Sciences, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Newcastle, Callaghan, NSW 2308 Australia</aff>
<aff id="Aff2"><label>2</label>
Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Queensland, Brisbane, QLD 4072 Australia</aff>
<aff id="Aff3"><label>3</label>
Baygen Institute, 6726 Szeged, Hungary</aff>
</contrib-group>
<pub-date pub-type="epub"><day>29</day>
<month>7</month>
<year>2009</year>
</pub-date>
<pub-date pub-type="ppub"><month>9</month>
<year>2009</year>
</pub-date>
<volume>230</volume>
<issue>4</issue>
<fpage>827</fpage>
<lpage>840</lpage>
<history><date date-type="received"><day>20</day>
<month>2</month>
<year>2009</year>
</date>
<date date-type="accepted"><day>9</day>
<month>7</month>
<year>2009</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s) 2009</copyright-statement>
</permissions>
<abstract xml:lang="EN"><p>Somatic embryogenesis (SE) is induced in vitro in <italic>Medicago truncatula</italic>
2HA by auxin and cytokinin but rarely in wild type Jemalong. The putative <italic>WUSCHEL</italic>
(<italic>MtWUS</italic>
), <italic>CLAVATA3</italic>
(<italic>MtCLV3</italic>
) and the <italic>WUSCHEL</italic>
-related homeobox gene <italic>WOX5</italic>
(<italic>MtWOX5)</italic>
were investigated in <italic>M. truncatula</italic>
(<italic>Mt</italic>
) and identified by the similarity to <italic>Arabidopsis WUS, CLV3</italic>
and <italic>WOX5</italic>
in amino acid sequence, phylogeny and in planta and in vitro expression patterns. <italic>MtWUS</italic>
was induced throughout embryogenic cultures by cytokinin after 24–48 h and maximum expression occurred after 1 week, which coincides with the induction of totipotent stem cells. During this period there was no <italic>MtCLV3</italic>
expression to suppress <italic>MtWUS. MtWUS</italic>
expression, as illustrated by promoter-GUS studies, subsequently localised to the embryo, and there was then the onset of <italic>MtCLV3</italic>
expression. This suggests that the expression of the putative <italic>MtCLV3</italic>
coincides with the WUS-CLAVATA feedback loop becoming operational. RNAi studies showed that <italic>MtWUS</italic>
expression is essential for callus and somatic embryo production. Based on the presence of <italic>MtWUS</italic>
promoter binding sites, <italic>MtWUS</italic>
may be required for the induction of <italic>MtSERF1,</italic>
postulated to have a key role in the signalling required for SE induced in 2HA. <italic>MtWOX5</italic>
expressed in auxin-induced root primordia and root meristems and appears to be involved in pluripotent stem cell induction. The evidence is discussed that the homeobox genes <italic>MtWUS</italic>
and <italic>MtWOX5</italic>
are “hijacked” for stem cell induction, which is key to somatic embryo and de novo root induction. In relation to SE, a role for <italic>WUS</italic>
in the signalling involved in induction is discussed.</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1007/s00425-009-0988-1) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group><title>Keywords</title>
<kwd><italic>Medicago</italic>
</kwd>
<kwd>Root development</kwd>
<kwd>Somatic embryogenesis</kwd>
<kwd>Stem cell formation</kwd>
<kwd><italic>WUSCHEL</italic>
</kwd>
<kwd><italic>WUSCHEL-</italic>
related homeobox genes</kwd>
</kwd-group>
<custom-meta-wrap><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Springer-Verlag 2009</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
</pmc>
</record>
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