Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source.
Identifieur interne : 000876 ( Ncbi/Checkpoint ); précédent : 000875; suivant : 000877Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source.
Auteurs : Seba George [Inde] ; K. JayachandranSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2009.
English descriptors
- KwdEn :
- Carbon (metabolism), Citrus sinensis, Culture Media, Fermentation, Glycolipids (analysis), Glycolipids (biosynthesis), Glycolipids (chemistry), Pseudomonas aeruginosa (metabolism), Surface Tension, Surface-Active Agents (analysis), Surface-Active Agents (chemistry), Surface-Active Agents (metabolism).
- MESH :
- chemical , analysis : Glycolipids, Surface-Active Agents.
- chemical , biosynthesis : Glycolipids.
- chemical , chemistry : Glycolipids, Surface-Active Agents.
- chemical , metabolism : Carbon, Surface-Active Agents.
- metabolism : Pseudomonas aeruginosa.
- Citrus sinensis, Culture Media, Fermentation, Surface Tension.
Abstract
The fermentative production of rhamnolipid biosurfactant from Pseudomonas aeruginosa MTCC 2297 was carried out by submerged fermentation using various cost-effective waste materials such as orange peelings, carrot peel waste, lime peelings, coconut oil cake, and banana waste. The orange peel was found to be the best substrate generating 9.18 g/l of rhamnolipid biosurfactant with a surface tension reduction up to 31.3 mN/m. The production was growth independent, and optimum conditions were standardized. The emulsifying activity was highest against kerosene (73.3%). Rhamnolipid components were purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, high-performance liquid chromatography and thin-layer chromatography. The major rhamnolipid components were characterized, by fast atom bombardment mass spectrometry, as a mixture of dirhamnolipids and monorhamnolipids.
DOI: 10.1007/s12010-008-8337-6
PubMed: 18716921
Affiliations:
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pubmed:18716921Le document en format XML
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<wicri:regionArea>School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills P.O, Athirampuzha, Kottayam District, Kerala 686 560</wicri:regionArea>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source.</title>
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<series><title level="j">Applied biochemistry and biotechnology</title>
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<term>Culture Media</term>
<term>Fermentation</term>
<term>Glycolipids (analysis)</term>
<term>Glycolipids (biosynthesis)</term>
<term>Glycolipids (chemistry)</term>
<term>Pseudomonas aeruginosa (metabolism)</term>
<term>Surface Tension</term>
<term>Surface-Active Agents (analysis)</term>
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<term>Surface-Active Agents (metabolism)</term>
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<front><div type="abstract" xml:lang="en">The fermentative production of rhamnolipid biosurfactant from Pseudomonas aeruginosa MTCC 2297 was carried out by submerged fermentation using various cost-effective waste materials such as orange peelings, carrot peel waste, lime peelings, coconut oil cake, and banana waste. The orange peel was found to be the best substrate generating 9.18 g/l of rhamnolipid biosurfactant with a surface tension reduction up to 31.3 mN/m. The production was growth independent, and optimum conditions were standardized. The emulsifying activity was highest against kerosene (73.3%). Rhamnolipid components were purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, high-performance liquid chromatography and thin-layer chromatography. The major rhamnolipid components were characterized, by fast atom bombardment mass spectrometry, as a mixture of dirhamnolipids and monorhamnolipids.</div>
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