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Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

Identifieur interne : 003026 ( Main/Merge ); précédent : 003025; suivant : 003027

Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

Auteurs : Robert J. Braddock [États-Unis] ; Charles R. Bryan [États-Unis]

Source :

Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2001.

RBID : Pascal:02-0115223

Descripteurs français

Pascal (Inist)
- Procédé extraction, Méthode analyse, Electrophorèse capillaire, Sous produit, Pulpe fruit, Peau, Albédo, Purification, Solution aqueuse, Citrus paradisi, Citrus sinensis, Limonine, Méthanol, Ethanol, Polygalacturonase, Chromatographie HPLC, Valorisation, Phlorine, Limonine glucoside.
Wicri :
- topic : Méthanol.

English descriptors

KwdEn :
- Albedo, Analysis method, Aqueous solution, By product, Capillary electrophoresis, Citrus paradisi, Citrus sinensis, Ethanol, Extraction process, Fruit pulp, HPLC chromatography, Limonin, Methanol, Polygalacturonase, Purification, Skin, Upgrading.

Abstract

Limonin glucoside (LG) and phlorin were extracted from citrus fruit tissues and assayed by capillary electrophoresis (CE). LG was determined in dried [1.20 ± 0.10 mg of dry weight (dw)] and wet peel residues (1.16 ± 0.04 mg of dw), orange juice finisher pulp (0.58 ± 0.03 mg of dw), dried grapefruit seeds (2.70 ± 0.15 mg of dw), and 50 °Brix molasses (2225 ± 68 mg/L). Phlorin was purified from orange peel residue and grapefruit albedo, and concentrations were determined in some citrus products. Phlorin and LG were extracted from residues with water/pectinase or with water solutions of methanol and ethanol. Efficient LG extraction from grapefruit seeds (2.40 ± 0.15 mg/g) was achieved with 50-65% methanol, solvent polarity P' 7-8. Extracts were purified and concentrated by adsorptive resins and HPLC to obtain 95% pure compounds of LG and phlorin. CE analysis did not require extract purification beyond filtration. LG and phlorin migrated as anions in electropherograms containing peaks representing other citrus flavonoids and limonoid glucosides.

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Pascal:02-0115223

Le document en format XML

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<author><name sortKey="Braddock, Robert J" sort="Braddock, Robert J" uniqKey="Braddock R" first="Robert J." last="Braddock">Robert J. Braddock</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research and Education Center, University of Florida, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
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<author><name sortKey="Bryan, Charles R" sort="Bryan, Charles R" uniqKey="Bryan C" first="Charles R." last="Bryan">Charles R. Bryan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research and Education Center, University of Florida, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
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<author><name sortKey="Bryan, Charles R" sort="Bryan, Charles R" uniqKey="Bryan C" first="Charles R." last="Bryan">Charles R. Bryan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research and Education Center, University of Florida, 700 Experiment Station Road</s1>
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<term>Analysis method</term>
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<term>By product</term>
<term>Capillary electrophoresis</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Ethanol</term>
<term>Extraction process</term>
<term>Fruit pulp</term>
<term>HPLC chromatography</term>
<term>Limonin</term>
<term>Methanol</term>
<term>Polygalacturonase</term>
<term>Purification</term>
<term>Skin</term>
<term>Upgrading</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Procédé extraction</term>
<term>Méthode analyse</term>
<term>Electrophorèse capillaire</term>
<term>Sous produit</term>
<term>Pulpe fruit</term>
<term>Peau</term>
<term>Albédo</term>
<term>Purification</term>
<term>Solution aqueuse</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Limonine</term>
<term>Méthanol</term>
<term>Ethanol</term>
<term>Polygalacturonase</term>
<term>Chromatographie HPLC</term>
<term>Valorisation</term>
<term>Phlorine</term>
<term>Limonine glucoside</term>
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<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Méthanol</term>
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<front><div type="abstract" xml:lang="en">Limonin glucoside (LG) and phlorin were extracted from citrus fruit tissues and assayed by capillary electrophoresis (CE). LG was determined in dried [1.20 ± 0.10 mg of dry weight (dw)] and wet peel residues (1.16 ± 0.04 mg of dw), orange juice finisher pulp (0.58 ± 0.03 mg of dw), dried grapefruit seeds (2.70 ± 0.15 mg of dw), and 50 °Brix molasses (2225 ± 68 mg/L). Phlorin was purified from orange peel residue and grapefruit albedo, and concentrations were determined in some citrus products. Phlorin and LG were extracted from residues with water/pectinase or with water solutions of methanol and ethanol. Efficient LG extraction from grapefruit seeds (2.40 ± 0.15 mg/g) was achieved with 50-65% methanol, solvent polarity P'  7-8. Extracts were purified and concentrated by adsorptive resins and HPLC to obtain 95% pure compounds of LG and phlorin. CE analysis did not require extract purification beyond filtration. LG and phlorin migrated as anions in electropherograms containing peaks representing other citrus flavonoids and limonoid glucosides.</div>
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<affiliations><list><country><li>États-Unis</li>
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<tree><country name="États-Unis"><noRegion><name sortKey="Braddock, Robert J" sort="Braddock, Robert J" uniqKey="Braddock R" first="Robert J." last="Braddock">Robert J. Braddock</name>
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<name sortKey="Bryan, Charles R" sort="Bryan, Charles R" uniqKey="Bryan C" first="Charles R." last="Bryan">Charles R. Bryan</name>
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Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri