Control of in vitro contamination of explants from greenhouse- and field-grown trees
Identifieur interne : 002E54 ( Main/Merge ); précédent : 002E53; suivant : 002E55Control of in vitro contamination of explants from greenhouse- and field-grown trees
Auteurs : Randall P. Niedz [États-Unis] ; Michael G. Bausher [États-Unis]Source :
- In vitro cellular & developmental biology. Plant [ 1054-5476 ] ; 2002.
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- Pascal (Inist)
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Abstract
Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l-1 Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l-1 Plant Preservative Mixture.
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Pascal:02-0599143Le document en format XML
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Bausher</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft.</s1><s2>Pierce, FL 34945-3030</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">02-0599143</idno><date when="2002">2002</date><idno type="stanalyst">PASCAL 02-0599143 INIST</idno><idno type="RBID">Pascal:02-0599143</idno><idno type="wicri:Area/PascalFrancis/Corpus">000798</idno><idno type="wicri:Area/PascalFrancis/Curation">000206</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000815</idno><idno type="wicri:doubleKey">1054-5476:2002:Niedz R:control:of:in</idno><idno type="wicri:Area/Main/Merge">002E54</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Control of in vitro contamination of explants from greenhouse- and field-grown trees</title><author><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft.</s1><s2>Pierce, FL 34945-3030</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Bausher, Michael G" sort="Bausher, Michael G" uniqKey="Bausher M" first="Michael G." last="Bausher">Michael G. Bausher</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft.</s1><s2>Pierce, FL 34945-3030</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j" type="main">In vitro cellular & developmental biology. Plant</title><title level="j" type="abbreviated">In vitro cell. dev. biol., Plant</title><idno type="ISSN">1054-5476</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">In vitro cellular & developmental biology. Plant</title><title level="j" type="abbreviated">In vitro cell. dev. biol., Plant</title><idno type="ISSN">1054-5476</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological contamination</term><term>Bud</term><term>Citrus sinensis</term><term>Comparative study</term><term>Disinfestation</term><term>Explant</term><term>Field experiment</term><term>Pretreatment</term><term>Protected cultivation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Contamination biologique</term><term>Explant</term><term>Bourgeon</term><term>Désinfestation</term><term>Prétraitement</term><term>Etude comparative</term><term>Culture protégée</term><term>Essai en champ</term><term>Citrus sinensis</term><term>Isothiazolone dérivé</term><term>Isocyanurate dérivé</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l<sup>-1</sup> Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l<sup>-1</sup> Plant Preservative Mixture.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name></region><name sortKey="Bausher, Michael G" sort="Bausher, Michael G" uniqKey="Bausher M" first="Michael G." last="Bausher">Michael G. Bausher</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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