Control of in vitro contamination of explants from greenhouse- and field-grown trees
Identifieur interne : 000798 ( PascalFrancis/Corpus ); précédent : 000797; suivant : 000799Control of in vitro contamination of explants from greenhouse- and field-grown trees
Auteurs : Randall P. Niedz ; Michael G. BausherSource :
- In vitro cellular & developmental biology. Plant [ 1054-5476 ] ; 2002.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l-1 Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l-1 Plant Preservative Mixture.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
pA |
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Format Inist (serveur)
NO : | PASCAL 02-0599143 INIST |
---|---|
ET : | Control of in vitro contamination of explants from greenhouse- and field-grown trees |
AU : | NIEDZ (Randall P.); BAUSHER (Michael G.) |
AF : | US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft./Pierce, FL 34945-3030/Etats-Unis (1 aut., 2 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | In vitro cellular & developmental biology. Plant; ISSN 1054-5476; Etats-Unis; Da. 2002; Vol. 38; No. 5; Pp. 468-471; Bibl. 12 ref. |
LA : | Anglais |
EA : | Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l-1 Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l-1 Plant Preservative Mixture. |
CC : | 002A32E09; 002A10I; 215 |
FD : | Contamination biologique; Explant; Bourgeon; Désinfestation; Prétraitement; Etude comparative; Culture protégée; Essai en champ; Citrus sinensis; Isothiazolone dérivé; Isocyanurate dérivé |
FG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Biocide |
ED : | Biological contamination; Explant; Bud; Disinfestation; Pretreatment; Comparative study; Protected cultivation; Field experiment; Citrus sinensis |
EG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Biocide |
SD : | Contaminación biológica; Explante; Yema; Desinfestación; Pretratamiento; Estudio comparativo; Cultivo protegido(invernadero); Ensayo en campo; Citrus sinensis |
LO : | INIST-15474A.354000105201470130 |
ID : | 02-0599143 |
Links to Exploration step
Pascal:02-0599143Le document en format XML
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<author><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name>
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<term>Citrus sinensis</term>
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<term>Explant</term>
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<term>Bourgeon</term>
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<term>Etude comparative</term>
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<front><div type="abstract" xml:lang="en">Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l<sup>-1</sup>
Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l<sup>-1</sup>
Plant Preservative Mixture.</div>
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Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l<sup>-1</sup>
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<s5>03</s5>
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<s5>04</s5>
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<s5>04</s5>
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<s5>04</s5>
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<server><NO>PASCAL 02-0599143 INIST</NO>
<ET>Control of in vitro contamination of explants from greenhouse- and field-grown trees</ET>
<AU>NIEDZ (Randall P.); BAUSHER (Michael G.)</AU>
<AF>US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft./Pierce, FL 34945-3030/Etats-Unis (1 aut., 2 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>In vitro cellular & developmental biology. Plant; ISSN 1054-5476; Etats-Unis; Da. 2002; Vol. 38; No. 5; Pp. 468-471; Bibl. 12 ref.</SO>
<LA>Anglais</LA>
<EA>Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l<sup>-1</sup>
Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l<sup>-1</sup>
Plant Preservative Mixture.</EA>
<CC>002A32E09; 002A10I; 215</CC>
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<ED>Biological contamination; Explant; Bud; Disinfestation; Pretreatment; Comparative study; Protected cultivation; Field experiment; Citrus sinensis</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Biocide</EG>
<SD>Contaminación biológica; Explante; Yema; Desinfestación; Pretratamiento; Estudio comparativo; Cultivo protegido(invernadero); Ensayo en campo; Citrus sinensis</SD>
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