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Control of in vitro contamination of explants from greenhouse- and field-grown trees

Identifieur interne : 000798 ( PascalFrancis/Corpus ); précédent : 000797; suivant : 000799

Control of in vitro contamination of explants from greenhouse- and field-grown trees

Auteurs : Randall P. Niedz ; Michael G. Bausher

Source :

RBID : Pascal:02-0599143

Descripteurs français

English descriptors

Abstract

Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l-1 Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l-1 Plant Preservative Mixture.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 1054-5476
A03   1    @0 In vitro cell. dev. biol., Plant
A05       @2 38
A06       @2 5
A08 01  1  ENG  @1 Control of in vitro contamination of explants from greenhouse- and field-grown trees
A11 01  1    @1 NIEDZ (Randall P.)
A11 02  1    @1 BAUSHER (Michael G.)
A14 01      @1 US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft. @2 Pierce, FL 34945-3030 @3 USA @Z 1 aut. @Z 2 aut.
A20       @1 468-471
A21       @1 2002
A23 01      @0 ENG
A43 01      @1 INIST @2 15474A @5 354000105201470130
A44       @0 0000 @1 © 2002 INIST-CNRS. All rights reserved.
A45       @0 12 ref.
A47 01  1    @0 02-0599143
A60       @1 P
A61       @0 A
A64 01  1    @0 In vitro cellular & developmental biology. Plant
A66 01      @0 USA
C01 01    ENG  @0 Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l-1 Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l-1 Plant Preservative Mixture.
C02 01  X    @0 002A32E09
C02 02  X    @0 002A10I
C02 03  X    @0 215
C03 01  X  FRE  @0 Contamination biologique @5 01
C03 01  X  ENG  @0 Biological contamination @5 01
C03 01  X  SPA  @0 Contaminación biológica @5 01
C03 02  X  FRE  @0 Explant @5 02
C03 02  X  ENG  @0 Explant @5 02
C03 02  X  SPA  @0 Explante @5 02
C03 03  X  FRE  @0 Bourgeon @5 03
C03 03  X  ENG  @0 Bud @5 03
C03 03  X  SPA  @0 Yema @5 03
C03 04  X  FRE  @0 Désinfestation @5 04
C03 04  X  ENG  @0 Disinfestation @5 04
C03 04  X  SPA  @0 Desinfestación @5 04
C03 05  X  FRE  @0 Prétraitement @5 05
C03 05  X  ENG  @0 Pretreatment @5 05
C03 05  X  SPA  @0 Pretratamiento @5 05
C03 06  X  FRE  @0 Etude comparative @5 06
C03 06  X  ENG  @0 Comparative study @5 06
C03 06  X  SPA  @0 Estudio comparativo @5 06
C03 07  X  FRE  @0 Culture protégée @5 07
C03 07  X  ENG  @0 Protected cultivation @5 07
C03 07  X  SPA  @0 Cultivo protegido(invernadero) @5 07
C03 08  X  FRE  @0 Essai en champ @5 08
C03 08  X  ENG  @0 Field experiment @5 08
C03 08  X  SPA  @0 Ensayo en campo @5 08
C03 09  X  FRE  @0 Citrus sinensis @2 NS @5 11
C03 09  X  ENG  @0 Citrus sinensis @2 NS @5 11
C03 09  X  SPA  @0 Citrus sinensis @2 NS @5 11
C03 10  X  FRE  @0 Isothiazolone dérivé @2 NK @4 INC @5 78
C03 11  X  FRE  @0 Isocyanurate dérivé @2 NK @4 INC @5 79
C07 01  X  FRE  @0 Rutaceae @2 NS
C07 01  X  ENG  @0 Rutaceae @2 NS
C07 01  X  SPA  @0 Rutaceae @2 NS
C07 02  X  FRE  @0 Dicotyledones @2 NS
C07 02  X  ENG  @0 Dicotyledones @2 NS
C07 02  X  SPA  @0 Dicotyledones @2 NS
C07 03  X  FRE  @0 Angiospermae @2 NS
C07 03  X  ENG  @0 Angiospermae @2 NS
C07 03  X  SPA  @0 Angiospermae @2 NS
C07 04  X  FRE  @0 Spermatophyta @2 NS
C07 04  X  ENG  @0 Spermatophyta @2 NS
C07 04  X  SPA  @0 Spermatophyta @2 NS
C07 05  X  FRE  @0 Agrume @5 41
C07 05  X  ENG  @0 Citrus fruit @5 41
C07 05  X  SPA  @0 Agrios @5 41
C07 06  X  FRE  @0 Biocide @5 51
C07 06  X  ENG  @0 Biocide @5 51
C07 06  X  SPA  @0 Biocida @5 51
N21       @1 351
N82       @1 PSI

Format Inist (serveur)

NO : PASCAL 02-0599143 INIST
ET : Control of in vitro contamination of explants from greenhouse- and field-grown trees
AU : NIEDZ (Randall P.); BAUSHER (Michael G.)
AF : US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft./Pierce, FL 34945-3030/Etats-Unis (1 aut., 2 aut.)
DT : Publication en série; Niveau analytique
SO : In vitro cellular & developmental biology. Plant; ISSN 1054-5476; Etats-Unis; Da. 2002; Vol. 38; No. 5; Pp. 468-471; Bibl. 12 ref.
LA : Anglais
EA : Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l-1 Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l-1 Plant Preservative Mixture.
CC : 002A32E09; 002A10I; 215
FD : Contamination biologique; Explant; Bourgeon; Désinfestation; Prétraitement; Etude comparative; Culture protégée; Essai en champ; Citrus sinensis; Isothiazolone dérivé; Isocyanurate dérivé
FG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Biocide
ED : Biological contamination; Explant; Bud; Disinfestation; Pretreatment; Comparative study; Protected cultivation; Field experiment; Citrus sinensis
EG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Biocide
SD : Contaminación biológica; Explante; Yema; Desinfestación; Pretratamiento; Estudio comparativo; Cultivo protegido(invernadero); Ensayo en campo; Citrus sinensis
LO : INIST-15474A.354000105201470130
ID : 02-0599143

Links to Exploration step

Pascal:02-0599143

Le document en format XML

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<div type="abstract" xml:lang="en">Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l
<sup>-1</sup>
Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l
<sup>-1</sup>
Plant Preservative Mixture.</div>
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<s0>Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l
<sup>-1</sup>
Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l
<sup>-1</sup>
Plant Preservative Mixture.</s0>
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<s5>01</s5>
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<s5>01</s5>
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<s5>02</s5>
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<s5>02</s5>
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<s5>02</s5>
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<s0>Bourgeon</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Bud</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Yema</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Désinfestation</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Disinfestation</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Desinfestación</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Prétraitement</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Pretreatment</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Pretratamiento</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Etude comparative</s0>
<s5>06</s5>
</fC03>
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<s5>06</s5>
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<s5>06</s5>
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<s5>07</s5>
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<s5>07</s5>
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<s5>07</s5>
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<s5>08</s5>
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<s5>08</s5>
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<s5>08</s5>
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<s2>NK</s2>
<s4>INC</s4>
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<s4>INC</s4>
<s5>79</s5>
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<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="01" i2="X" l="SPA">
<s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="02" i2="X" l="FRE">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Agrume</s0>
<s5>41</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Citrus fruit</s0>
<s5>41</s5>
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<NO>PASCAL 02-0599143 INIST</NO>
<ET>Control of in vitro contamination of explants from greenhouse- and field-grown trees</ET>
<AU>NIEDZ (Randall P.); BAUSHER (Michael G.)</AU>
<AF>US Department of Agriculture, ARS, USHRL, 2001 South Rock Road, Ft./Pierce, FL 34945-3030/Etats-Unis (1 aut., 2 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>In vitro cellular & developmental biology. Plant; ISSN 1054-5476; Etats-Unis; Da. 2002; Vol. 38; No. 5; Pp. 468-471; Bibl. 12 ref.</SO>
<LA>Anglais</LA>
<EA>Controlling fungal and bacterial contamination of woody plant material can be extremely difficult. Isothiazolone biocides and sodium dichloroisocyanurate have been used singly and in combination to reduce microbial contamination in bud explants derived from greenhouse- and field-grown citrus trees. Explants from greenhouse-grown trees were effectively disinfested (< 5% vs. 85% contamination) using a 'standard' disinfestation (SD) procedure followed by culture on medium containing 5 ml l
<sup>-1</sup>
Plant Preservative Mixture, or by the SD procedure but substituting 300 ppm sodium dichloroisocyanurate for 48 h for the treatment with 1.05% NaOCl in the SD procedure. Disinfestation of explants from field-grown trees was less effective than explants from the greenhouse, but was improved (10% vs. 47% contamination) by using a combination of the SD procedure with 1.05% NaOCl, or 100 or 300 ppm sodium dichloroisocyanurate followed by culture onto medium containing 5 ml l
<sup>-1</sup>
Plant Preservative Mixture.</EA>
<CC>002A32E09; 002A10I; 215</CC>
<FD>Contamination biologique; Explant; Bourgeon; Désinfestation; Prétraitement; Etude comparative; Culture protégée; Essai en champ; Citrus sinensis; Isothiazolone dérivé; Isocyanurate dérivé</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Biocide</FG>
<ED>Biological contamination; Explant; Bud; Disinfestation; Pretreatment; Comparative study; Protected cultivation; Field experiment; Citrus sinensis</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Biocide</EG>
<SD>Contaminación biológica; Explante; Yema; Desinfestación; Pretratamiento; Estudio comparativo; Cultivo protegido(invernadero); Ensayo en campo; Citrus sinensis</SD>
<LO>INIST-15474A.354000105201470130</LO>
<ID>02-0599143</ID>
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