Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene.
Identifieur interne : 001E38 ( Main/Merge ); précédent : 001E37; suivant : 001E39Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene.
Auteurs : M N R. Baig [République populaire de Chine] ; An Yu ; Wenwu Guo ; Xiuxin DengSource :
- Genome [ 0831-2796 ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Alkyl and Aryl Transferases, DNA, Complementary, DNA, Mitochondrial.
- genetics : Chromosomes, Artificial, Bacterial, Citrus.
- Gene Library, Geranylgeranyl-Diphosphate Geranylgeranyltransferase.
Abstract
Two deep-coverage Bacterial Artificial Chromosome (BAC) libraries of Citrus sinensis (L.) Osbeck 'Cara Cara' navel orange and Citrus reticulata (L.) Blanco 'Egan No. 1' Ponkan mandarin, which belong to the two most important species of the Citrus genus, have been constructed and characterized to facilitate gene cloning and to analyze variety-specific genome composition. The C. sinensis BAC library consists of 36 000 clones with negligible false-positive clones and an estimated average insert size of 126 kb covering ~4.5 x 109 bp and thus providing an 11.8-fold coverage of haploid genome equivalents, whereas the C. reticulata library consists of 21 000 clones also with negligible false-positive clones and an estimated average of 120 kb covering ~2.5 x 109 bp representing a 6.6-fold coverage of haploid genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBAC536 vector, and organellar DNA sequences. Screening has been performed by Southern hybridization of BAC filters, which results in <0.5% chloroplast DNA contamination and no mitochondrial DNA contamination in both libraries. Eight and five positive clones harboring the gene encoding Phytoene synthase (Psy (EC 2.5.1.32)) were identified from the C. sinensis and C. reticulata libraries, respectively, using the filter hybridization procedure. These results suggest that the two BAC libraries are useful tools for the isolation of functional genes and advanced genomics research in the two important species C. sinensis and C. reticulata. Resources, high-density filters, individual clones, and whole libraries are available for public distribution and are accessible at the National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University.
DOI: 10.1139/g09-017
PubMed: 19448728
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000957
- to stream PubMed, to step Curation: 000957
- to stream PubMed, to step Checkpoint: 000957
- to stream Ncbi, to step Merge: 000A02
- to stream Ncbi, to step Curation: 000A02
- to stream Ncbi, to step Checkpoint: 000A02
Links to Exploration step
pubmed:19448728Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene.</title>
<author><name sortKey="Baig, M N R" sort="Baig, M N R" uniqKey="Baig M" first="M N R" last="Baig">M N R. Baig</name>
<affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea>
<wicri:noRegion>Wuhan 430070</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yu, An" sort="Yu, An" uniqKey="Yu A" first="An" last="Yu">An Yu</name>
</author>
<author><name sortKey="Guo, Wenwu" sort="Guo, Wenwu" uniqKey="Guo W" first="Wenwu" last="Guo">Wenwu Guo</name>
</author>
<author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:19448728</idno>
<idno type="pmid">19448728</idno>
<idno type="doi">10.1139/g09-017</idno>
<idno type="wicri:Area/PubMed/Corpus">000957</idno>
<idno type="wicri:Area/PubMed/Curation">000957</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000957</idno>
<idno type="wicri:Area/Ncbi/Merge">000A02</idno>
<idno type="wicri:Area/Ncbi/Curation">000A02</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000A02</idno>
<idno type="wicri:doubleKey">0831-2796:2009:Baig M:construction:and:characterization</idno>
<idno type="wicri:Area/Main/Merge">001E38</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene.</title>
<author><name sortKey="Baig, M N R" sort="Baig, M N R" uniqKey="Baig M" first="M N R" last="Baig">M N R. Baig</name>
<affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070</wicri:regionArea>
<wicri:noRegion>Wuhan 430070</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yu, An" sort="Yu, An" uniqKey="Yu A" first="An" last="Yu">An Yu</name>
</author>
<author><name sortKey="Guo, Wenwu" sort="Guo, Wenwu" uniqKey="Guo W" first="Wenwu" last="Guo">Wenwu Guo</name>
</author>
<author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name>
</author>
</analytic>
<series><title level="j">Genome</title>
<idno type="ISSN">0831-2796</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkyl and Aryl Transferases (genetics)</term>
<term>Chromosomes, Artificial, Bacterial (genetics)</term>
<term>Citrus (genetics)</term>
<term>DNA, Complementary (genetics)</term>
<term>DNA, Mitochondrial (genetics)</term>
<term>Gene Library</term>
<term>Geranylgeranyl-Diphosphate Geranylgeranyltransferase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alkyl and Aryl Transferases</term>
<term>DNA, Complementary</term>
<term>DNA, Mitochondrial</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Artificial, Bacterial</term>
<term>Citrus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Library</term>
<term>Geranylgeranyl-Diphosphate Geranylgeranyltransferase</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Two deep-coverage Bacterial Artificial Chromosome (BAC) libraries of Citrus sinensis (L.) Osbeck 'Cara Cara' navel orange and Citrus reticulata (L.) Blanco 'Egan No. 1' Ponkan mandarin, which belong to the two most important species of the Citrus genus, have been constructed and characterized to facilitate gene cloning and to analyze variety-specific genome composition. The C. sinensis BAC library consists of 36 000 clones with negligible false-positive clones and an estimated average insert size of 126 kb covering ~4.5 x 109 bp and thus providing an 11.8-fold coverage of haploid genome equivalents, whereas the C. reticulata library consists of 21 000 clones also with negligible false-positive clones and an estimated average of 120 kb covering ~2.5 x 109 bp representing a 6.6-fold coverage of haploid genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBAC536 vector, and organellar DNA sequences. Screening has been performed by Southern hybridization of BAC filters, which results in <0.5% chloroplast DNA contamination and no mitochondrial DNA contamination in both libraries. Eight and five positive clones harboring the gene encoding Phytoene synthase (Psy (EC 2.5.1.32)) were identified from the C. sinensis and C. reticulata libraries, respectively, using the filter hybridization procedure. These results suggest that the two BAC libraries are useful tools for the isolation of functional genes and advanced genomics research in the two important species C. sinensis and C. reticulata. Resources, high-density filters, individual clones, and whole libraries are available for public distribution and are accessible at the National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E38 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001E38 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:19448728 |texte= Construction and characterization of two Citrus BAC libraries and identification of clones containing the phytoene synthase gene. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:19448728" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
![]() | This area was generated with Dilib version V0.6.25. | ![]() |