Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source.
Identifieur interne : 000957 ( PubMed/Checkpoint ); précédent : 000956; suivant : 000958Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source.
Auteurs : Seba George [Inde] ; K. JayachandranSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2009.
English descriptors
- KwdEn :
- Carbon (metabolism), Citrus sinensis, Culture Media, Fermentation, Glycolipids (analysis), Glycolipids (biosynthesis), Glycolipids (chemistry), Pseudomonas aeruginosa (metabolism), Surface Tension, Surface-Active Agents (analysis), Surface-Active Agents (chemistry), Surface-Active Agents (metabolism).
- MESH :
- chemical , analysis : Glycolipids, Surface-Active Agents.
- chemical , biosynthesis : Glycolipids.
- chemical , chemistry : Glycolipids, Surface-Active Agents.
- chemical , metabolism : Carbon, Surface-Active Agents.
- metabolism : Pseudomonas aeruginosa.
- Citrus sinensis, Culture Media, Fermentation, Surface Tension.
Abstract
The fermentative production of rhamnolipid biosurfactant from Pseudomonas aeruginosa MTCC 2297 was carried out by submerged fermentation using various cost-effective waste materials such as orange peelings, carrot peel waste, lime peelings, coconut oil cake, and banana waste. The orange peel was found to be the best substrate generating 9.18 g/l of rhamnolipid biosurfactant with a surface tension reduction up to 31.3 mN/m. The production was growth independent, and optimum conditions were standardized. The emulsifying activity was highest against kerosene (73.3%). Rhamnolipid components were purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, high-performance liquid chromatography and thin-layer chromatography. The major rhamnolipid components were characterized, by fast atom bombardment mass spectrometry, as a mixture of dirhamnolipids and monorhamnolipids.
DOI: 10.1007/s12010-008-8337-6
PubMed: 18716921
Affiliations:
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The orange peel was found to be the best substrate generating 9.18 g/l of rhamnolipid biosurfactant with a surface tension reduction up to 31.3 mN/m. The production was growth independent, and optimum conditions were standardized. The emulsifying activity was highest against kerosene (73.3%). Rhamnolipid components were purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, high-performance liquid chromatography and thin-layer chromatography. The major rhamnolipid components were characterized, by fast atom bombardment mass spectrometry, as a mixture of dirhamnolipids and monorhamnolipids.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18716921</PMID><DateCreated><Year>2010</Year><Month>2</Month><Day>3</Day></DateCreated><DateCompleted><Year>2010</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2010</Year><Month>2</Month><Day>3</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>158</Volume><Issue>3</Issue><PubDate><Year>2009</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl. Biochem. Biotechnol.</ISOAbbreviation></Journal><ArticleTitle>Analysis of rhamnolipid biosurfactants produced through submerged fermentation using orange fruit peelings as sole carbon source.</ArticleTitle><Pagination><MedlinePgn>694-705</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-008-8337-6</ELocationID><Abstract><AbstractText>The fermentative production of rhamnolipid biosurfactant from Pseudomonas aeruginosa MTCC 2297 was carried out by submerged fermentation using various cost-effective waste materials such as orange peelings, carrot peel waste, lime peelings, coconut oil cake, and banana waste. The orange peel was found to be the best substrate generating 9.18 g/l of rhamnolipid biosurfactant with a surface tension reduction up to 31.3 mN/m. The production was growth independent, and optimum conditions were standardized. The emulsifying activity was highest against kerosene (73.3%). Rhamnolipid components were purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, high-performance liquid chromatography and thin-layer chromatography. The major rhamnolipid components were characterized, by fast atom bombardment mass spectrometry, as a mixture of dirhamnolipids and monorhamnolipids.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>George</LastName><ForeName>Seba</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills P.O, Athirampuzha, Kottayam District, Kerala 686 560, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jayachandran</LastName><ForeName>K</ForeName><Initials>K</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>Aug</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006017">Glycolipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013501">Surface-Active Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C418382">rhamnolipid</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="Y">Citrus sinensis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="Y">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006017" MajorTopicYN="N">Glycolipids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011550" MajorTopicYN="N">Pseudomonas aeruginosa</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013500" MajorTopicYN="N">Surface Tension</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013501" MajorTopicYN="N">Surface-Active Agents</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>6</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>7</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>8</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>6</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>8</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18716921</ArticleId><ArticleId IdType="doi">10.1007/s12010-008-8337-6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Jayachandran, K" sort="Jayachandran, K" uniqKey="Jayachandran K" first="K" last="Jayachandran">K. Jayachandran</name></noCountry><country name="Inde"><noRegion><name sortKey="George, Seba" sort="George, Seba" uniqKey="George S" first="Seba" last="George">Seba George</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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