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Molecular characterization of two glutathione peroxidase genes of Panax ginseng and their expression analysis against environmental stresses.

Identifieur interne : 000A01 ( Main/Exploration ); précédent : 000A00; suivant : 000A02

Molecular characterization of two glutathione peroxidase genes of Panax ginseng and their expression analysis against environmental stresses.

Auteurs : Yu-Jin Kim [Corée du Sud] ; Moon-Gi Jang [Corée du Sud] ; Hae-Yong Noh [Corée du Sud] ; Hye-Jin Lee [Corée du Sud] ; Johan Sukweenadhi [Corée du Sud] ; Jong-Hak Kim [Corée du Sud] ; Se-Yeong Kim [Corée du Sud] ; Woo-Saeng Kwon [Corée du Sud] ; Deok-Chun Yang [Corée du Sud]

Source :

Gene [ 1879-0038 ] ; 2014.

RBID : pubmed:24269671

English descriptors

KwdEn :
- Amino Acid Sequence, Base Sequence, DNA Primers, DNA, Complementary (genetics), Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Genes, Plant, Glutathione Peroxidase (genetics), Molecular Sequence Data, Open Reading Frames, Panax (enzymology), Panax (genetics), Real-Time Polymerase Chain Reaction, Sequence Homology, Amino Acid, Stress, Physiological.
MESH :
- chemical , genetics : DNA, Complementary, Glutathione Peroxidase.
- chemical : DNA Primers.
- enzymology : Panax.
- genetics : Panax.
- Amino Acid Sequence, Base Sequence, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Genes, Plant, Molecular Sequence Data, Open Reading Frames, Real-Time Polymerase Chain Reaction, Sequence Homology, Amino Acid, Stress, Physiological.

Abstract

Glutathione peroxidases (GPXs) are a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). GPX catalyzes the reduction of hydrogen peroxide (H2O2) or organic hydroperoxides to water or alcohols by reduced glutathione. The presence of GPXs in plants has been reported by several groups, but the roles of individual members of this family in a single plant species have not been studied. Two GPX cDNAs were isolated and characterized from the embryogenic callus of Panax ginseng. The two cDNAs had an open reading frame (ORF) of 723 and 681bp with a deduced amino acid sequence of 240 and 226 residues, respectively. The calculated molecular mass of the matured proteins are approximately 26.4kDa or 25.7kDa with a predicated isoelectric point of 9.16 or 6.11, respectively. The two PgGPXs were elevated strongly by salt stress and chilling stress in a ginseng seedling. In addition, the two PgGPXs showed different responses against biotic stress. The positive responses of PgGPX to the environmental stimuli suggested that ginseng GPX may help to protect against environmental stresses.

DOI: 10.1016/j.gene.2013.10.071
PubMed: 24269671

Affiliations:

Corée du Sud

Le document en format XML

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<author><name sortKey="Kim, Yu Jin" sort="Kim, Yu Jin" uniqKey="Kim Y" first="Yu-Jin" last="Kim">Yu-Jin Kim</name>
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<author><name sortKey="Yang, Deok Chun" sort="Yang, Deok Chun" uniqKey="Yang D" first="Deok-Chun" last="Yang">Deok-Chun Yang</name>
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<author><name sortKey="Yang, Deok Chun" sort="Yang, Deok Chun" uniqKey="Yang D" first="Deok-Chun" last="Yang">Deok-Chun Yang</name>
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<term>Base Sequence</term>
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<term>Gene Expression Regulation, Enzymologic</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genes, Plant</term>
<term>Glutathione Peroxidase (genetics)</term>
<term>Molecular Sequence Data</term>
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<term>Stress, Physiological</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">Glutathione peroxidases (GPXs) are a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). GPX catalyzes the reduction of hydrogen peroxide (H2O2) or organic hydroperoxides to water or alcohols by reduced glutathione. The presence of GPXs in plants has been reported by several groups, but the roles of individual members of this family in a single plant species have not been studied. Two GPX cDNAs were isolated and characterized from the embryogenic callus of Panax ginseng. The two cDNAs had an open reading frame (ORF) of 723 and 681bp with a deduced amino acid sequence of 240 and 226 residues, respectively. The calculated molecular mass of the matured proteins are approximately 26.4kDa or 25.7kDa with a predicated isoelectric point of 9.16 or 6.11, respectively. The two PgGPXs were elevated strongly by salt stress and chilling stress in a ginseng seedling. In addition, the two PgGPXs showed different responses against biotic stress. The positive responses of PgGPX to the environmental stimuli suggested that ginseng GPX may help to protect against environmental stresses.</div>
</front>
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<name sortKey="Kim, Se Yeong" sort="Kim, Se Yeong" uniqKey="Kim S" first="Se-Yeong" last="Kim">Se-Yeong Kim</name>
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Molecular characterization of two glutathione peroxidase genes of Panax ginseng and their expression analysis against environmental stresses.

Molecular characterization of two glutathione peroxidase genes of Panax ginseng and their expression analysis against environmental stresses.

Source :

English descriptors

Abstract

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