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Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C

Identifieur interne : 003280 ( Main/Exploration ); précédent : 003279; suivant : 003281

Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C

Auteurs : Shi-Ming Jiang ; Zhao-Hui Xu ; Yan Zhang ; Xian-Min Shi

Source :

World Journal of Gastroenterology [ 1007-9327 ] ; 1997.

RBID : PMC:4842870

Abstract

AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.

METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.

RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.

CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842870

DOI: 10.3748/wjg.v3.i3.150
PubMed: 27239129
PubMed Central: 4842870

Affiliations:

Le document en format XML

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<author><name sortKey="Jiang, Shi Ming" sort="Jiang, Shi Ming" uniqKey="Jiang S" first="Shi-Ming" last="Jiang">Shi-Ming Jiang</name>
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<author><name sortKey="Xu, Zhao Hui" sort="Xu, Zhao Hui" uniqKey="Xu Z" first="Zhao-Hui" last="Xu">Zhao-Hui Xu</name>
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<author><name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name>
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<author><name sortKey="Shi, Xian Min" sort="Shi, Xian Min" uniqKey="Shi X" first="Xian-Min" last="Shi">Xian-Min Shi</name>
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<author><name sortKey="Jiang, Shi Ming" sort="Jiang, Shi Ming" uniqKey="Jiang S" first="Shi-Ming" last="Jiang">Shi-Ming Jiang</name>
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<author><name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name>
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<author><name sortKey="Shi, Xian Min" sort="Shi, Xian Min" uniqKey="Shi X" first="Xian-Min" last="Shi">Xian-Min Shi</name>
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<series><title level="j">World Journal of Gastroenterology</title>
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<front><div type="abstract" xml:lang="en"><p>AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.</p>
<p>METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.</p>
<p>RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.</p>
<p>CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.</p>
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<tree><noCountry><name sortKey="Jiang, Shi Ming" sort="Jiang, Shi Ming" uniqKey="Jiang S" first="Shi-Ming" last="Jiang">Shi-Ming Jiang</name>
<name sortKey="Shi, Xian Min" sort="Shi, Xian Min" uniqKey="Shi X" first="Xian-Min" last="Shi">Xian-Min Shi</name>
<name sortKey="Xu, Zhao Hui" sort="Xu, Zhao Hui" uniqKey="Xu Z" first="Zhao-Hui" last="Xu">Zhao-Hui Xu</name>
<name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name>
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Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C

Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C

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