Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C
Identifieur interne : 000273 ( Pmc/Curation ); précédent : 000272; suivant : 000274Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C
Auteurs : Shi-Ming Jiang ; Zhao-Hui Xu ; Yan Zhang ; Xian-Min ShiSource :
- World Journal of Gastroenterology [ 1007-9327 ] ; 1997.
Abstract
AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.
METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.
RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.
CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.
Url:
DOI: 10.3748/wjg.v3.i3.150
PubMed: 27239129
PubMed Central: 4842870
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000274
Links to Exploration step
PMC:4842870Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C</title>
<author><name sortKey="Jiang, Shi Ming" sort="Jiang, Shi Ming" uniqKey="Jiang S" first="Shi-Ming" last="Jiang">Shi-Ming Jiang</name>
</author>
<author><name sortKey="Xu, Zhao Hui" sort="Xu, Zhao Hui" uniqKey="Xu Z" first="Zhao-Hui" last="Xu">Zhao-Hui Xu</name>
</author>
<author><name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name>
</author>
<author><name sortKey="Shi, Xian Min" sort="Shi, Xian Min" uniqKey="Shi X" first="Xian-Min" last="Shi">Xian-Min Shi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27239129</idno>
<idno type="pmc">4842870</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842870</idno>
<idno type="RBID">PMC:4842870</idno>
<idno type="doi">10.3748/wjg.v3.i3.150</idno>
<date when="1997">1997</date>
<idno type="wicri:Area/Pmc/Corpus">000274</idno>
<idno type="wicri:Area/Pmc/Curation">000273</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C</title>
<author><name sortKey="Jiang, Shi Ming" sort="Jiang, Shi Ming" uniqKey="Jiang S" first="Shi-Ming" last="Jiang">Shi-Ming Jiang</name>
</author>
<author><name sortKey="Xu, Zhao Hui" sort="Xu, Zhao Hui" uniqKey="Xu Z" first="Zhao-Hui" last="Xu">Zhao-Hui Xu</name>
</author>
<author><name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name>
</author>
<author><name sortKey="Shi, Xian Min" sort="Shi, Xian Min" uniqKey="Shi X" first="Xian-Min" last="Shi">Xian-Min Shi</name>
</author>
</analytic>
<series><title level="j">World Journal of Gastroenterology</title>
<idno type="ISSN">1007-9327</idno>
<idno type="eISSN">2219-2840</idno>
<imprint><date when="1997">1997</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.</p>
<p>METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.</p>
<p>RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.</p>
<p>CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">World J Gastroenterol</journal-id>
<journal-id journal-id-type="iso-abbrev">World J. Gastroenterol</journal-id>
<journal-id journal-id-type="publisher-id">WJG</journal-id>
<journal-title-group><journal-title>World Journal of Gastroenterology</journal-title>
</journal-title-group>
<issn pub-type="ppub">1007-9327</issn>
<issn pub-type="epub">2219-2840</issn>
<publisher><publisher-name>Baishideng Publishing Group Inc</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">27239129</article-id>
<article-id pub-id-type="pmc">4842870</article-id>
<article-id pub-id-type="other">jWJG.v3.i3.pg150</article-id>
<article-id pub-id-type="doi">10.3748/wjg.v3.i3.150</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Jiang</surname>
<given-names>Shi-Ming</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Xu</surname>
<given-names>Zhao-Hui</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Yan</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Shi</surname>
<given-names>Xian-Min</given-names>
</name>
</contrib>
<aff>Shi-Ming Jiang, Zhao-Hui Xu, Biology Department, Shandong Normal University, Jinan 250014, Shandong Province, China</aff>
<aff>Yan Zhang, Shanghai Institute of Cell Biology, Academia Sinica</aff>
<aff>Xian-Min Shi, Biology Department, Xiamen University</aff>
</contrib-group>
<author-notes><fn><p>Author contributions: All authors contributed equally to the work.</p>
<p>Correspondence to: Dr. Shi-Ming Jiang, Biology Department, Shandong Normal University, Jinan 250014, Shandong Province, China</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><day>15</day>
<month>9</month>
<year>1997</year>
</pub-date>
<pub-date pub-type="epub"><day>15</day>
<month>9</month>
<year>1997</year>
</pub-date>
<volume>3</volume>
<issue>3</issue>
<fpage>150</fpage>
<lpage>152</lpage>
<history><date date-type="received"><day>26</day>
<month>10</month>
<year>1996</year>
</date>
<date date-type="rev-recd"><day>19</day>
<month>12</month>
<year>1996</year>
</date>
<date date-type="accepted"><day>22</day>
<month>1</month>
<year>1997</year>
</date>
</history>
<permissions><copyright-statement>©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.</copyright-statement>
<copyright-year>1997</copyright-year>
</permissions>
<abstract><p>AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.</p>
<p>METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.</p>
<p>RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.</p>
<p>CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.</p>
</abstract>
<kwd-group><kwd>Liver neoplasms</kwd>
<kwd>Carcinoma, hepatocellular</kwd>
<kwd>Cryopreservation</kwd>
<kwd>Tumor cells, cultured</kwd>
<kwd>Phenotype</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000273 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000273 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:4842870 |texte= Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:27239129" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
This area was generated with Dilib version V0.6.25. |