Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination
Identifieur interne : 002123 ( Main/Exploration ); précédent : 002122; suivant : 002124Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination
Auteurs : M. A. Hurtado [Espagne] ; T. Ansaloni [Espagne] ; S. Cros-Arteil [France] ; J. A. Jacas [Espagne] ; M. Navajas [France]Source :
- Annals of Applied Biology [ 0003-4746 ] ; 2008-10.
English descriptors
- KwdEn :
Abstract
Tetranychus urticae is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction–restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.
Url:
DOI: 10.1111/j.1744-7348.2008.00250.x
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001606
- to stream Istex, to step Curation: 001605
- to stream Istex, to step Checkpoint: 000559
- to stream Main, to step Merge: 002185
- to stream Main, to step Curation: 002123
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination</title>
<author><name sortKey="Hurtado, M A" sort="Hurtado, M A" uniqKey="Hurtado M" first="M. A." last="Hurtado">M. A. Hurtado</name>
</author>
<author><name sortKey="Ansaloni, T" sort="Ansaloni, T" uniqKey="Ansaloni T" first="T." last="Ansaloni">T. Ansaloni</name>
</author>
<author><name sortKey="Cros Rteil, S" sort="Cros Rteil, S" uniqKey="Cros Rteil S" first="S." last="Cros-Arteil">S. Cros-Arteil</name>
</author>
<author><name sortKey="Jacas, J A" sort="Jacas, J A" uniqKey="Jacas J" first="J. A." last="Jacas">J. A. Jacas</name>
</author>
<author><name sortKey="Navajas, M" sort="Navajas, M" uniqKey="Navajas M" first="M." last="Navajas">M. Navajas</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:04C247C2F485CC2E8DA905F479B76AAE02798369</idno>
<date when="2008" year="2008">2008</date>
<idno type="doi">10.1111/j.1744-7348.2008.00250.x</idno>
<idno type="url">https://api.istex.fr/document/04C247C2F485CC2E8DA905F479B76AAE02798369/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001606</idno>
<idno type="wicri:Area/Istex/Curation">001605</idno>
<idno type="wicri:Area/Istex/Checkpoint">000559</idno>
<idno type="wicri:doubleKey">0003-4746:2008:Hurtado M:sequence:analysis:of</idno>
<idno type="wicri:Area/Main/Merge">002185</idno>
<idno type="wicri:Area/Main/Curation">002123</idno>
<idno type="wicri:Area/Main/Exploration">002123</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination</title>
<author><name sortKey="Hurtado, M A" sort="Hurtado, M A" uniqKey="Hurtado M" first="M. A." last="Hurtado">M. A. Hurtado</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana</wicri:regionArea>
<wicri:noRegion>Castelló de la Plana</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Espagne</country>
</affiliation>
</author>
<author><name sortKey="Ansaloni, T" sort="Ansaloni, T" uniqKey="Ansaloni T" first="T." last="Ansaloni">T. Ansaloni</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana</wicri:regionArea>
<wicri:noRegion>Castelló de la Plana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cros Rteil, S" sort="Cros Rteil, S" uniqKey="Cros Rteil S" first="S." last="Cros-Arteil">S. Cros-Arteil</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INRA‐CBGP, Campus International de Baillarguet, Montferrier‐sur‐Lez Cedex</wicri:regionArea>
<wicri:noRegion>Montferrier‐sur‐Lez Cedex</wicri:noRegion>
<wicri:noRegion>Montferrier‐sur‐Lez Cedex</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jacas, J A" sort="Jacas, J A" uniqKey="Jacas J" first="J. A." last="Jacas">J. A. Jacas</name>
<affiliation wicri:level="1"><country xml:lang="fr">Espagne</country>
<wicri:regionArea>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana</wicri:regionArea>
<wicri:noRegion>Castelló de la Plana</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Navajas, M" sort="Navajas, M" uniqKey="Navajas M" first="M." last="Navajas">M. Navajas</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>INRA‐CBGP, Campus International de Baillarguet, Montferrier‐sur‐Lez Cedex</wicri:regionArea>
<wicri:noRegion>Montferrier‐sur‐Lez Cedex</wicri:noRegion>
<wicri:noRegion>Montferrier‐sur‐Lez Cedex</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Annals of Applied Biology</title>
<idno type="ISSN">0003-4746</idno>
<idno type="eISSN">1744-7348</idno>
<imprint><publisher>Blackwell Publishing Ltd</publisher>
<pubPlace>Oxford, UK</pubPlace>
<date type="published" when="2008-10">2008-10</date>
<biblScope unit="volume">153</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="167">167</biblScope>
<biblScope unit="page" to="174">174</biblScope>
</imprint>
<idno type="ISSN">0003-4746</idno>
</series>
<idno type="istex">04C247C2F485CC2E8DA905F479B76AAE02798369</idno>
<idno type="DOI">10.1111/j.1744-7348.2008.00250.x</idno>
<idno type="ArticleID">AAB250</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0003-4746</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus reticulata</term>
<term>Panonychus citri</term>
<term>Tetranychus spp</term>
<term>internal transcribed spacer</term>
<term>molecular species discrimination</term>
<term>pest management</term>
<term>phylogenetic relationships</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Tetranychus urticae is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction–restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>France</li>
</country>
</list>
<tree><country name="Espagne"><noRegion><name sortKey="Hurtado, M A" sort="Hurtado, M A" uniqKey="Hurtado M" first="M. A." last="Hurtado">M. A. Hurtado</name>
</noRegion>
<name sortKey="Ansaloni, T" sort="Ansaloni, T" uniqKey="Ansaloni T" first="T." last="Ansaloni">T. Ansaloni</name>
<name sortKey="Hurtado, M A" sort="Hurtado, M A" uniqKey="Hurtado M" first="M. A." last="Hurtado">M. A. Hurtado</name>
<name sortKey="Jacas, J A" sort="Jacas, J A" uniqKey="Jacas J" first="J. A." last="Jacas">J. A. Jacas</name>
</country>
<country name="France"><noRegion><name sortKey="Cros Rteil, S" sort="Cros Rteil, S" uniqKey="Cros Rteil S" first="S." last="Cros-Arteil">S. Cros-Arteil</name>
</noRegion>
<name sortKey="Navajas, M" sort="Navajas, M" uniqKey="Navajas M" first="M." last="Navajas">M. Navajas</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002123 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002123 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:04C247C2F485CC2E8DA905F479B76AAE02798369 |texte= Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination }}
![]() | This area was generated with Dilib version V0.6.25. | ![]() |