Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination
Identifieur interne : 001606 ( Istex/Corpus ); précédent : 001605; suivant : 001607Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination
Auteurs : M. A. Hurtado ; T. Ansaloni ; S. Cros-Arteil ; J. A. Jacas ; M. NavajasSource :
- Annals of Applied Biology [ 0003-4746 ] ; 2008-10.
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Abstract
Tetranychus urticae is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction–restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.
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DOI: 10.1111/j.1744-7348.2008.00250.x
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Ansaloni</name><affiliation><mods:affiliation>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</mods:affiliation></affiliation></author><author><name sortKey="Cros Rteil, S" sort="Cros Rteil, S" uniqKey="Cros Rteil S" first="S." last="Cros-Arteil">S. Cros-Arteil</name><affiliation><mods:affiliation>INRA‐CBGP, Campus International de Baillarguet, Montferrier‐sur‐Lez Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Jacas, J A" sort="Jacas, J A" uniqKey="Jacas J" first="J. A." last="Jacas">J. A. Jacas</name><affiliation><mods:affiliation>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</mods:affiliation></affiliation></author><author><name sortKey="Navajas, M" sort="Navajas, M" uniqKey="Navajas M" first="M." last="Navajas">M. 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This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction–restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>M.A. Hurtado</name><affiliations><json:string>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</json:string></affiliations></json:item><json:item><name>T. Ansaloni</name><affiliations><json:string>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</json:string></affiliations></json:item><json:item><name>S. Cros‐Arteil</name><affiliations><json:string>INRA‐CBGP, Campus International de Baillarguet, Montferrier‐sur‐Lez Cedex, France</json:string></affiliations></json:item><json:item><name>J.A. 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This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. 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This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction–restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Citrus reticulata</term></item><item><term>internal transcribed spacer</term></item><item><term>molecular species discrimination</term></item><item><term>Panonychus citri</term></item><item><term>pest management</term></item><item><term>phylogenetic relationships</term></item><item><term>Tetranychus spp</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-10">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/04C247C2F485CC2E8DA905F479B76AAE02798369/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1744-7348</doi><issn type="print">0003-4746</issn><issn type="electronic">1744-7348</issn><idGroup><id type="product" value="AAB"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ANNALS OF APPLIED BIOLOGY">Annals of Applied Biology</title></titleGroup></publicationMeta><publicationMeta level="part" position="10002"><doi origin="wiley">10.1111/aab.2008.153.issue-2</doi><numberingGroup><numbering type="journalVolume" number="153">153</numbering><numbering type="journalIssue" number="2">2</numbering></numberingGroup><coverDate startDate="2008-10">October 2008</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="3" status="forIssue"><doi origin="wiley">10.1111/j.1744-7348.2008.00250.x</doi><idGroup><id type="unit" value="AAB250"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="tocHeading1">RESEARCH ARTICLES</title></titleGroup><copyright>© 2008 The Authors Journal compilation © 2008 Association of Applied Biologists</copyright><eventGroup><event type="firstOnline" date="2008-05-27"></event><event type="publishedOnlineFinalForm" date="2008-10-02"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.5 mode:FullText source:HeaderRef result:HeaderRef" date="2010-04-06"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-14"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="167">167</numbering><numbering type="pageLast" number="174">174</numbering></numberingGroup><correspondenceTo>Mónica Hurtado Ruiz, Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, E‐12071‐Castelló de la Plana, Spain.
Email: <email>mhurtado@camn.uji.es</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:AAB.AAB250.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: 24 May 2007; revised versionaccepted: 6 March 2008.</unparsedEditorialHistory><countGroup><count type="figureTotal" number="2"></count><count type="tableTotal" number="5"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="31"></count><count type="wordTotal" number="0"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination</title><title type="shortAuthors">M.A. Hurtado <i>et al.</i></title><title type="short"><b>Sequence analysis of the ribosomal ITS in spider mites in Spanish citrus</b></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>M.A.</givenNames><familyName>Hurtado</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>T.</givenNames><familyName>Ansaloni</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>S.</givenNames><familyName>Cros‐Arteil</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>J.A.</givenNames><familyName>Jacas</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>M.</givenNames><familyName>Navajas</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="ES"><unparsedAffiliation>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>INRA‐CBGP, Campus International de Baillarguet, Montferrier‐sur‐Lez Cedex, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1"><i>Citrus reticulata</i></keyword><keyword xml:id="k2">internal transcribed spacer</keyword><keyword xml:id="k3">molecular species discrimination</keyword><keyword xml:id="k4"><i>Panonychus citri</i></keyword><keyword xml:id="k5">pest management</keyword><keyword xml:id="k6">phylogenetic relationships</keyword><keyword xml:id="k7"><i>Tetranychus </i>spp</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p> <i>Tetranychus urticae </i>is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of <i>T.</i> <i>urticae</i>, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of <i>T.</i> <i>urticae</i>, <i>Tetranychus turkestani</i>, <i>Tetranychus evansi</i>, <i>Tetranychus ludeni </i>and <i>Panonychus </i><i>citri </i>have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of <i>T.</i> <i>urticae </i>obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction–restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Sequence analysis of the ribosomal ITS in spider mites in Spanish citrus</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination</title></titleInfo><name type="personal"><namePart type="given">M.A.</namePart><namePart type="family">Hurtado</namePart><affiliation>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</affiliation><affiliation>E-mail: mhurtado@camn.uji.es</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Ansaloni</namePart><affiliation>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Cros‐Arteil</namePart><affiliation>INRA‐CBGP, Campus International de Baillarguet, Montferrier‐sur‐Lez Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.A.</namePart><namePart type="family">Jacas</namePart><affiliation>Departament de Ciències Agràries i del Medi Natural, Universitat Jaume I, Campus del Riu Sec, Castelló de la Plana, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Navajas</namePart><affiliation>INRA‐CBGP, Campus International de Baillarguet, Montferrier‐sur‐Lez Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2008-10</dateIssued><edition>Received: 24 May 2007; revised versionaccepted: 6 March 2008.</edition><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">2</extent><extent unit="tables">5</extent><extent unit="references">31</extent></physicalDescription><abstract lang="en">Tetranychus urticae is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species‐discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2 regions are much more conserved than the corresponding rDNA ITS1. The high homogeneity of the ITS2 sequence observed among the specimens of T. urticae obtained from the same ecoregion makes this DNA sequence an excellent tool for species discrimination. ITS sequences differentiate not only species but also specimens from different geographical origin. Furthermore, polymerase chain reaction–restriction fragment length polymorphism analysis of the ITS2 proved adequate for a quick screening of high numbers of field samples.</abstract><subject lang="en"><genre>keywords</genre><topic>Citrus reticulata</topic><topic>internal transcribed spacer</topic><topic>molecular species discrimination</topic><topic>Panonychus citri</topic><topic>pest management</topic><topic>phylogenetic relationships</topic><topic>Tetranychus spp</topic></subject><relatedItem type="host"><titleInfo><title>Annals of Applied Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0003-4746</identifier><identifier type="eISSN">1744-7348</identifier><identifier type="DOI">10.1111/(ISSN)1744-7348</identifier><identifier type="PublisherID">AAB</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>153</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>167</start><end>174</end><total>8</total></extent></part></relatedItem><identifier type="istex">04C247C2F485CC2E8DA905F479B76AAE02798369</identifier><identifier type="DOI">10.1111/j.1744-7348.2008.00250.x</identifier><identifier type="ArticleID">AAB250</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2008 The Authors Journal compilation © 2008 Association of Applied Biologists</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/04C247C2F485CC2E8DA905F479B76AAE02798369/enrichments/multicat</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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