OrangerV1, Main, Exploration, bibRecord, 002E63

Capillary electrophoresis analysis of orange juice pectinesterases

Identifieur interne : 002E63 ( Main/Exploration ); précédent : 002E62; suivant : 002E64

Capillary electrophoresis analysis of orange juice pectinesterases

Auteurs : Robert J. Braddock [États-Unis] ; Charles R. Bryan [États-Unis] ; Jacqueline K. Burns [États-Unis]

Source :

Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2001.

RBID : Pascal:01-0177851

Descripteurs français

Pascal (Inist)
- Méthode analyse, Pectinesterase, Jus d'orange, Electrophorèse capillaire, Stabilité thermique, Qualité production, Flaveur, Traitement thermique, Propriété chimique, Citrus sinensis, Inactivation, Caractérisation, Masse moléculaire.

English descriptors

KwdEn :
- Analysis method, Capillary electrophoresis, Characterization, Chemical properties, Citrus sinensis, Flavor, Heat treatment, Inactivation, Molecular weight, Orange juice, Pectinesterase, Production quality, Thermal stability.

Abstract

Pectinesterase (PE) was extracted from orange juice and pulp with 1 M NaCl, desalted, and separated using capillary electrophoresis (CE) gel procedures (CE-SDS-CGE) and isoelectric focusing (CE-IEF). PE resolved as a single peak using noncoated fused silica columns with CE-SDS-CGE. CE-IEF separation of PE required acryloylaminoethoxyethanol-coated columns, which had limited stability. Thermal stability of PE extracts before and after heating at 75 °C for 30 min and at 95 °C for 5 min established heat labile and heat stabile fractions with identical PE migration times by CE-SDS-CGE or CE-IEF. Peak magnitude decreased to a constant value as heating time increased at 75 °C. Regression analysis of CE-SDS-CGE peak migration times of molecular weight (MW) standards estimated both heat labile and heat stable PE at MW 36 900. Traditional SDS-PAGE gel separation of MW standards and active PE isolated by IEF allowed estimation of MW 36 000. CE-SDS-CGE allowed presumptive, but not quantitative, detection of active PE in fresh juice.

Affiliations:

États-Unis

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000917
to stream PascalFrancis, to step Curation: 000087
to stream PascalFrancis, to step Checkpoint: 000903
to stream Main, to step Merge: 003059
to stream Main, to step Curation: 002E63

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Capillary electrophoresis analysis of orange juice pectinesterases</title>
<author><name sortKey="Braddock, Robert J" sort="Braddock, Robert J" uniqKey="Braddock R" first="Robert J." last="Braddock">Robert J. Braddock</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, Citrus Research and Education Center, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bryan, Charles R" sort="Bryan, Charles R" uniqKey="Bryan C" first="Charles R." last="Bryan">Charles R. Bryan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, Citrus Research and Education Center, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K." last="Burns">Jacqueline K. Burns</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, Citrus Research and Education Center, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">01-0177851</idno>
<date when="2001">2001</date>
<idno type="stanalyst">PASCAL 01-0177851 INIST</idno>
<idno type="RBID">Pascal:01-0177851</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000917</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000087</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000903</idno>
<idno type="wicri:doubleKey">0021-8561:2001:Braddock R:capillary:electrophoresis:analysis</idno>
<idno type="wicri:Area/Main/Merge">003059</idno>
<idno type="wicri:Area/Main/Curation">002E63</idno>
<idno type="wicri:Area/Main/Exploration">002E63</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Capillary electrophoresis analysis of orange juice pectinesterases</title>
<author><name sortKey="Braddock, Robert J" sort="Braddock, Robert J" uniqKey="Braddock R" first="Robert J." last="Braddock">Robert J. Braddock</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, Citrus Research and Education Center, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bryan, Charles R" sort="Bryan, Charles R" uniqKey="Bryan C" first="Charles R." last="Bryan">Charles R. Bryan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, Citrus Research and Education Center, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K." last="Burns">Jacqueline K. Burns</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Florida, Citrus Research and Education Center, 700 Experiment Station Road</s1>
<s2>Lake Alfred, Florida 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of agricultural and food chemistry : (Print)</title>
<title level="j" type="abbreviated">J. agric. food chem. : (Print)</title>
<idno type="ISSN">0021-8561</idno>
<imprint><date when="2001">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of agricultural and food chemistry : (Print)</title>
<title level="j" type="abbreviated">J. agric. food chem. : (Print)</title>
<idno type="ISSN">0021-8561</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis method</term>
<term>Capillary electrophoresis</term>
<term>Characterization</term>
<term>Chemical properties</term>
<term>Citrus sinensis</term>
<term>Flavor</term>
<term>Heat treatment</term>
<term>Inactivation</term>
<term>Molecular weight</term>
<term>Orange juice</term>
<term>Pectinesterase</term>
<term>Production quality</term>
<term>Thermal stability</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Méthode analyse</term>
<term>Pectinesterase</term>
<term>Jus d'orange</term>
<term>Electrophorèse capillaire</term>
<term>Stabilité thermique</term>
<term>Qualité production</term>
<term>Flaveur</term>
<term>Traitement thermique</term>
<term>Propriété chimique</term>
<term>Citrus sinensis</term>
<term>Inactivation</term>
<term>Caractérisation</term>
<term>Masse moléculaire</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Pectinesterase (PE) was extracted from orange juice and pulp with 1 M NaCl, desalted, and separated using capillary electrophoresis (CE) gel procedures (CE-SDS-CGE) and isoelectric focusing (CE-IEF). PE resolved as a single peak using noncoated fused silica columns with CE-SDS-CGE. CE-IEF separation of PE required acryloylaminoethoxyethanol-coated columns, which had limited stability. Thermal stability of PE extracts before and after heating at 75 °C for 30 min and at 95 °C for 5 min established heat labile and heat stabile fractions with identical PE migration times by CE-SDS-CGE or CE-IEF. Peak magnitude decreased to a constant value as heating time increased at 75 °C. Regression analysis of CE-SDS-CGE peak migration times of molecular weight (MW) standards estimated both heat labile and heat stable PE at MW 36 900. Traditional SDS-PAGE gel separation of MW standards and active PE isolated by IEF allowed estimation of MW 36 000. CE-SDS-CGE allowed presumptive, but not quantitative, detection of active PE in fresh juice.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Braddock, Robert J" sort="Braddock, Robert J" uniqKey="Braddock R" first="Robert J." last="Braddock">Robert J. Braddock</name>
</noRegion>
<name sortKey="Bryan, Charles R" sort="Bryan, Charles R" uniqKey="Bryan C" first="Charles R." last="Bryan">Charles R. Bryan</name>
<name sortKey="Burns, Jacqueline K" sort="Burns, Jacqueline K" uniqKey="Burns J" first="Jacqueline K." last="Burns">Jacqueline K. Burns</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E63 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002E63 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:01-0177851
   |texte=   Capillary electrophoresis analysis of orange juice pectinesterases
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Capillary electrophoresis analysis of orange juice pectinesterases

Capillary electrophoresis analysis of orange juice pectinesterases

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri