Clarification of juice by thermolabile Valencia pectinmethylesterase is accelerated by cations
Identifieur interne : 002C77 ( Main/Exploration ); précédent : 002C76; suivant : 002C78Clarification of juice by thermolabile Valencia pectinmethylesterase is accelerated by cations
Auteurs : L. Wicker [États-Unis] ; J. L. Ackerley [États-Unis] ; M. Corredig [États-Unis]Source :
- Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2002.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Calcium.
English descriptors
- KwdEn :
Abstract
Pectinmethylesterase (PME) was isolated from Valencia orange pulp and added to reconstituted juice at 1.2 units/mL of juice in the presence or absence of cations at 4.2 or 16.7 mM. The percent transmittance (%T) of control juices with no added PME or cation did not clarify. The %T of juices with added PME and added cation was 45-55% by the second day. Increases in the average particle size was observed with PME- or cation-added juices and preceded increases in %T. Most likely, cations displaced PME from an inactive pectin substrate complex and increased clarification. PME, in the absence of cations, increased particle size but did not affect %T, suggesting a direct interaction of PME with cloud particles.
Affiliations:
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Calcium</term>
<term>Citrus sinensis</term>
<term>Clarifying</term>
<term>Colloidal dispersion</term>
<term>Enzymatic activity</term>
<term>Fruit juice</term>
<term>Manufacturing process</term>
<term>Orange juice</term>
<term>Particle size</term>
<term>Pectin</term>
<term>Pectinesterase</term>
<term>Strontium</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Procédé fabrication</term>
<term>Clarification</term>
<term>Jus fruit</term>
<term>Jus d'orange</term>
<term>Pectinesterase</term>
<term>Activité enzymatique</term>
<term>Dimension particule</term>
<term>Dispersion colloïdale</term>
<term>Citrus sinensis</term>
<term>Calcium</term>
<term>Strontium</term>
<term>Pectine</term>
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<front><div type="abstract" xml:lang="en">Pectinmethylesterase (PME) was isolated from Valencia orange pulp and added to reconstituted juice at 1.2 units/mL of juice in the presence or absence of cations at 4.2 or 16.7 mM. The percent transmittance (%T) of control juices with no added PME or cation did not clarify. The %T of juices with added PME and added cation was 45-55% by the second day. Increases in the average particle size was observed with PME- or cation-added juices and preceded increases in %T. Most likely, cations displaced PME from an inactive pectin substrate complex and increased clarification. PME, in the absence of cations, increased particle size but did not affect %T, suggesting a direct interaction of PME with cloud particles.</div>
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