Selection for aluminium tolerance in embryogenic lines of Citrus sinensis L. and C. aurantium L.
Identifieur interne : 002B79 ( Main/Exploration ); précédent : 002B78; suivant : 002B80Selection for aluminium tolerance in embryogenic lines of Citrus sinensis L. and C. aurantium L.
Auteurs : Bao Toan Nguyen [Viêt Nam] ; Pierre C. DeberghSource :
- Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen) [ 1373-7503 ] ; 2002.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Culture Media.
- chemical , pharmacology : Aluminum.
- chemical , toxicity : Aluminum.
- drug effects : Citrus, Regeneration.
- embryology : Citrus.
- genetics : Citrus.
- growth & development : Citrus.
- Culture Techniques, Dose-Response Relationship, Drug, Selection, Genetic, Time Factors.
Abstract
The Citrus cultivars 'Amargo' and 'Pineapple' produce embryogenic cultures on two types of sugars, galactose and lactose. Two percent lactose is optimal for both cultivars; embryogenic callus is produced 8-10 weeks after inoculation. Whereas 2% galactose is optimal for 'Amargo', it is 3% for 'Pineapple'. Three types of embryos are recognized: whitish, greenish and heart shaped. In 8 to 10 week old cultures we do also observe a black spotted type of embryos. A high percentage of plant conversion for both cultivars is observed on a medium containing 30 mg/l of adenine and 2 mg/l of GA3 for 'Amargo'; for 'Pineapple' it is necessary to add BA. We tested two techniques for selection of Al-tolerant lines: one on stagnant liquid medium, and another on shaken liquid medium. The second technique gives a more serious selection pressure; there are almost no surviving calli. In 'Amargo' more calli survived than in 'Pineapple'. Selected calli had to be subcultured nine times to determine their stability. Four callus types of the two cultivars grew normally on selective media containing 0, 400, 1000 or 2000 mumol of aluminium.
PubMed: 12491964
Affiliations:
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Le document en format XML
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Debergh</name></author></analytic><series><title level="j">Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)</title><idno type="ISSN">1373-7503</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminum (pharmacology)</term><term>Aluminum (toxicity)</term><term>Citrus (drug effects)</term><term>Citrus (embryology)</term><term>Citrus (genetics)</term><term>Citrus (growth & development)</term><term>Culture Media (chemistry)</term><term>Culture Techniques</term><term>Dose-Response Relationship, Drug</term><term>Regeneration (drug effects)</term><term>Selection, Genetic</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Aluminum</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Aluminum</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Citrus</term><term>Regeneration</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Culture Techniques</term><term>Dose-Response Relationship, Drug</term><term>Selection, Genetic</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Citrus cultivars 'Amargo' and 'Pineapple' produce embryogenic cultures on two types of sugars, galactose and lactose. Two percent lactose is optimal for both cultivars; embryogenic callus is produced 8-10 weeks after inoculation. Whereas 2% galactose is optimal for 'Amargo', it is 3% for 'Pineapple'. Three types of embryos are recognized: whitish, greenish and heart shaped. In 8 to 10 week old cultures we do also observe a black spotted type of embryos. A high percentage of plant conversion for both cultivars is observed on a medium containing 30 mg/l of adenine and 2 mg/l of GA3 for 'Amargo'; for 'Pineapple' it is necessary to add BA. We tested two techniques for selection of Al-tolerant lines: one on stagnant liquid medium, and another on shaken liquid medium. The second technique gives a more serious selection pressure; there are almost no surviving calli. In 'Amargo' more calli survived than in 'Pineapple'. Selected calli had to be subcultured nine times to determine their stability. Four callus types of the two cultivars grew normally on selective media containing 0, 400, 1000 or 2000 mumol of aluminium.</div></front></TEI><affiliations><list><country><li>Viêt Nam</li></country></list><tree><noCountry><name sortKey="Debergh, Pierre C" sort="Debergh, Pierre C" uniqKey="Debergh P" first="Pierre C" last="Debergh">Pierre C. Debergh</name></noCountry><country name="Viêt Nam"><noRegion><name sortKey="Nguyen, Bao Toan" sort="Nguyen, Bao Toan" uniqKey="Nguyen B" first="Bao Toan" last="Nguyen">Bao Toan Nguyen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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