OrangerV1, Main, Exploration, bibRecord, 002B33

Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Identifieur interne : 002B33 ( Main/Exploration ); précédent : 002B32; suivant : 002B34

Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Auteurs : A. K. Kobayashi [Brésil] ; J. C. Bespalhok [Brésil] ; L. F. P. Pereira [Brésil] ; L. G. E. Vieira [Brésil]

Source :

Plant cell, tissue and organ culture [ 0167-6857 ] ; 2003.

RBID : Pascal:03-0479314

Abstract

An efficient system for in vitro plant regeneration from thin transversal stem sections explants (1-2 mm) using mature tissues of sweet orange cv. Pera was developed. Explants were cultured in different media to evaluate the frequency of regeneration and size of buds. A high percentage of explants (54% with 3.1 buds/explant) producing large buds (1-4 mm) was observed when the explants were cultivated for 2 weeks on Murashige and Skoog medium and then transferred to Woody Plant medium (WPM). Both media were supplemented with 1.8 μM 6-benzylaminopurine and 0.7 μM gibberellic acid. Adventitious buds were regenerated into whole plants by in vitro shoot-tip grafting. Regenerated plants started to flower after 12 months in the greenhouse, confirming their mature nature.

Affiliations:

Brésil

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000727
to stream PascalFrancis, to step Curation: 000277
to stream PascalFrancis, to step Checkpoint: 000712
to stream Main, to step Merge: 002C98
to stream Main, to step Curation: 002B33

Le document en format XML

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<front><div type="abstract" xml:lang="en">An efficient system for in vitro plant regeneration from thin transversal stem sections explants (1-2 mm) using mature tissues of sweet orange cv. Pera was developed. Explants were cultured in different media to evaluate the frequency of regeneration and size of buds. A high percentage of explants (54% with 3.1 buds/explant) producing large buds (1-4 mm) was observed when the explants were cultivated for 2 weeks on Murashige and Skoog medium and then transferred to Woody Plant medium (WPM). Both media were supplemented with 1.8 μM 6-benzylaminopurine and 0.7 μM gibberellic acid. Adventitious buds were regenerated into whole plants by in vitro shoot-tip grafting. Regenerated plants started to flower after 12 months in the greenhouse, confirming their mature nature.</div>
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Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri