OrangerV1, Main, Exploration, bibRecord, 001968

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Identifieur interne : 001968 ( Main/Exploration ); précédent : 001967; suivant : 001969

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Auteurs : Asokan Bagavan [Inde] ; ABDUL ABDUL RAHUMAN [Inde] ; Chinnaperumal Kamaraj [Inde] ; NAVEEN KUMAR KAUSHIK [Inde] ; Dinesh Mohanakrishnan [Inde] ; Dinkar Sahal [Inde]

Source :

Parasitology research : (1987) [ 0932-0113 ] ; 2011.

RBID : Pascal:11-0226704

Descripteurs français

Pascal (Inist)
- Activité, Paludisme, Parasite, Plasmodium falciparum.

English descriptors

KwdEn :
- Activity, Malaria, Parasite, Plasmodium falciparum.

Abstract

The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. The aim of the study was to discover effective plant extracts against Plasmodium falciparum. In the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Citrus sinensis (peel), Leucas aspera, Ocimum sanctum, Phyllanthus acidus (leaf), Terminalia chebula (seed) were tested for their antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of P. falciparum which was cultured following the candle-jar method. Antimalarial evaluations of daily replacement of culture medium containing CQ and different plant crude extracts were performed on 96-well plates at 37°C for 24 and 48 h. Parasitemia was determined microscopically on thin-film Giemsa-stained preparations. Plant extracts were tested for their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on human laryngeal cancer cell line (HEp-2) and normal cell line (Vero). Out of the 25 extracts tested, six showed good (IC_so 4.76-22.76 μg/mL), 15 exhibited moderate (IC₅₀ 31.42-88.03 μg/mL), while four displayed mild (IC₅₀> 100 μg/mL) antiplasmodial activity. The leaf ethyl acetate and methanol extracts of L. aspera; ethyl acetate, acetone, and methanol extracts of P. acidus; and seed acetone extract of T. chebula had good antiplasmodial activity (IC_so=7.81, 22.76, 9.37, 14.65, 12.68, and 4.76 μg/mL) with selectivity indices 5.43, 2.04, 4.88, 3.35, 3.42, and 9.97 for HEp-2 and >5.79, >2.20, >11.75, >3.41, >3.94, and >7.38 for Vero cells, respectively. These analyses have revealed for the first time that the components present in the solvent extracts of L. aspera, P. acidus, and T. chebula have antiplasmodial activity. The high antiplasmodial activity observed make these plants good candidates for isolation of anti-protozoal compounds which could serve as new lead structures for drug development.

Affiliations:

Inde

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Le document en format XML

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<author><name sortKey="Bagavan, Asokan" sort="Bagavan, Asokan" uniqKey="Bagavan A" first="Asokan" last="Bagavan">Asokan Bagavan</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Antiplasmodial activity of botanical extracts against Plasmodium falciparum</title>
<author><name sortKey="Bagavan, Asokan" sort="Bagavan, Asokan" uniqKey="Bagavan A" first="Asokan" last="Bagavan">Asokan Bagavan</name>
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<author><name sortKey="Naveen Kumar Kaushik" sort="Naveen Kumar Kaushik" uniqKey="Naveen Kumar Kaushik" last="Naveen Kumar Kaushik">NAVEEN KUMAR KAUSHIK</name>
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<author><name sortKey="Sahal, Dinkar" sort="Sahal, Dinkar" uniqKey="Sahal D" first="Dinkar" last="Sahal">Dinkar Sahal</name>
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<series><title level="j" type="main">Parasitology research : (1987)</title>
<title level="j" type="abbreviated">Parasitol. res. : (1987)</title>
<idno type="ISSN">0932-0113</idno>
<imprint><date when="2011">2011</date>
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<seriesStmt><title level="j" type="main">Parasitology research : (1987)</title>
<title level="j" type="abbreviated">Parasitol. res. : (1987)</title>
<idno type="ISSN">0932-0113</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Activity</term>
<term>Malaria</term>
<term>Parasite</term>
<term>Plasmodium falciparum</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Activité</term>
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<term>Parasite</term>
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<front><div type="abstract" xml:lang="en">The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. The aim of the study was to discover effective plant extracts against Plasmodium falciparum. In the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Citrus sinensis (peel), Leucas aspera, Ocimum sanctum, Phyllanthus acidus (leaf), Terminalia chebula (seed) were tested for their antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of P. falciparum which was cultured following the candle-jar method. Antimalarial evaluations of daily replacement of culture medium containing CQ and different plant crude extracts were performed on 96-well plates at 37°C for 24 and 48 h. Parasitemia was determined microscopically on thin-film Giemsa-stained preparations. Plant extracts were tested for their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on human laryngeal cancer cell line (HEp-2) and normal cell line (Vero). Out of the 25 extracts tested, six showed good (IC<sub>so</sub>
 4.76-22.76 μg/mL), 15 exhibited moderate (IC<sub>50</sub>
 31.42-88.03 μg/mL), while four displayed mild (IC<sub>50</sub>
> 100 μg/mL) antiplasmodial activity. The leaf ethyl acetate and methanol extracts of L. aspera; ethyl acetate, acetone, and methanol extracts of P. acidus; and seed acetone extract of T. chebula had good antiplasmodial activity (IC<sub>so</sub>
=7.81, 22.76, 9.37, 14.65, 12.68, and 4.76 μg/mL) with selectivity indices 5.43, 2.04, 4.88, 3.35, 3.42, and 9.97 for HEp-2 and >5.79, >2.20, >11.75, >3.41, >3.94, and >7.38 for Vero cells, respectively. These analyses have revealed for the first time that the components present in the solvent extracts of L. aspera, P. acidus, and T. chebula have antiplasmodial activity. The high antiplasmodial activity observed make these plants good candidates for isolation of anti-protozoal compounds which could serve as new lead structures for drug development.</div>
</front>
</TEI>
<affiliations><list><country><li>Inde</li>
</country>
</list>
<tree><country name="Inde"><noRegion><name sortKey="Bagavan, Asokan" sort="Bagavan, Asokan" uniqKey="Bagavan A" first="Asokan" last="Bagavan">Asokan Bagavan</name>
</noRegion>
<name sortKey="Abdul Abdul Rahuman" sort="Abdul Abdul Rahuman" uniqKey="Abdul Abdul Rahuman" last="Abdul Abdul Rahuman">ABDUL ABDUL RAHUMAN</name>
<name sortKey="Kamaraj, Chinnaperumal" sort="Kamaraj, Chinnaperumal" uniqKey="Kamaraj C" first="Chinnaperumal" last="Kamaraj">Chinnaperumal Kamaraj</name>
<name sortKey="Mohanakrishnan, Dinesh" sort="Mohanakrishnan, Dinesh" uniqKey="Mohanakrishnan D" first="Dinesh" last="Mohanakrishnan">Dinesh Mohanakrishnan</name>
<name sortKey="Naveen Kumar Kaushik" sort="Naveen Kumar Kaushik" uniqKey="Naveen Kumar Kaushik" last="Naveen Kumar Kaushik">NAVEEN KUMAR KAUSHIK</name>
<name sortKey="Sahal, Dinkar" sort="Sahal, Dinkar" uniqKey="Sahal D" first="Dinkar" last="Sahal">Dinkar Sahal</name>
</country>
</tree>
</affiliations>
</record>

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Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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