OrangerV1, PascalFrancis, Curation, bibRecord, 000824

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Identifieur interne : 000824 ( PascalFrancis/Curation ); précédent : 000823; suivant : 000825

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Auteurs : Asokan Bagavan [Inde] ; ABDUL ABDUL RAHUMAN [Inde] ; Chinnaperumal Kamaraj [Inde] ; NAVEEN KUMAR KAUSHIK [Inde] ; Dinesh Mohanakrishnan [Inde] ; Dinkar Sahal [Inde]

Source :

Parasitology research : (1987) [ 0932-0113 ] ; 2011.

RBID : Pascal:11-0226704

Descripteurs français

Pascal (Inist)
- Activité, Paludisme, Parasite, Plasmodium falciparum.

English descriptors

KwdEn :
- Activity, Malaria, Parasite, Plasmodium falciparum.

Abstract

The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. The aim of the study was to discover effective plant extracts against Plasmodium falciparum. In the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Citrus sinensis (peel), Leucas aspera, Ocimum sanctum, Phyllanthus acidus (leaf), Terminalia chebula (seed) were tested for their antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of P. falciparum which was cultured following the candle-jar method. Antimalarial evaluations of daily replacement of culture medium containing CQ and different plant crude extracts were performed on 96-well plates at 37°C for 24 and 48 h. Parasitemia was determined microscopically on thin-film Giemsa-stained preparations. Plant extracts were tested for their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on human laryngeal cancer cell line (HEp-2) and normal cell line (Vero). Out of the 25 extracts tested, six showed good (IC_so 4.76-22.76 μg/mL), 15 exhibited moderate (IC₅₀ 31.42-88.03 μg/mL), while four displayed mild (IC₅₀> 100 μg/mL) antiplasmodial activity. The leaf ethyl acetate and methanol extracts of L. aspera; ethyl acetate, acetone, and methanol extracts of P. acidus; and seed acetone extract of T. chebula had good antiplasmodial activity (IC_so=7.81, 22.76, 9.37, 14.65, 12.68, and 4.76 μg/mL) with selectivity indices 5.43, 2.04, 4.88, 3.35, 3.42, and 9.97 for HEp-2 and >5.79, >2.20, >11.75, >3.41, >3.94, and >7.38 for Vero cells, respectively. These analyses have revealed for the first time that the components present in the solvent extracts of L. aspera, P. acidus, and T. chebula have antiplasmodial activity. The high antiplasmodial activity observed make these plants good candidates for isolation of anti-protozoal compounds which could serve as new lead structures for drug development.

A01	`01`	`1`		`@0 0932-0113`
A02	`01`			`@0 PARREZ`
A03		`1`		`@0 Parasitol. res. : (1987)`
A05				`@2 108`
A06				`@2 5`
A08	`01`	`1`	`ENG`	`@1 Antiplasmodial activity of botanical extracts against Plasmodium falciparum`
A11	`01`	`1`		`@1 BAGAVAN (Asokan)`
A11	`02`	`1`		`@1 ABDUL ABDUL RAHUMAN`
A11	`03`	`1`		`@1 KAMARAJ (Chinnaperumal)`
A11	`04`	`1`		`@1 NAVEEN KUMAR KAUSHIK`
A11	`05`	`1`		`@1 MOHANAKRISHNAN (Dinesh)`
A11	`06`	`1`		`@1 SAHAL (Dinkar)`
A14	`01`			`@1 Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509 @2 Vellore District, Tamil Nadu @3 IND @Z 1 aut. @Z 2 aut. @Z 3 aut.`
A14	`02`			`@1 Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg @2 New Delhi 110067 @3 IND @Z 4 aut. @Z 5 aut. @Z 6 aut.`
A20				`@1 1099-1109`
A21				`@1 2011`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 5859 @5 354000191467460040`
A44				`@0 0000 @1 © 2011 INIST-CNRS. All rights reserved.`
A45				`@0 2 p.`
A47	`01`	`1`		`@0 11-0226704`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Parasitology research : (1987)`
A66	`01`			`@0 DEU`
C01	`01`		`ENG`	@0 The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. The aim of the study was to discover effective plant extracts against Plasmodium falciparum. In the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Citrus sinensis (peel), Leucas aspera, Ocimum sanctum, Phyllanthus acidus (leaf), Terminalia chebula (seed) were tested for their antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of P. falciparum which was cultured following the candle-jar method. Antimalarial evaluations of daily replacement of culture medium containing CQ and different plant crude extracts were performed on 96-well plates at 37°C for 24 and 48 h. Parasitemia was determined microscopically on thin-film Giemsa-stained preparations. Plant extracts were tested for their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on human laryngeal cancer cell line (HEp-2) and normal cell line (Vero). Out of the 25 extracts tested, six showed good (IC_so 4.76-22.76 μg/mL), 15 exhibited moderate (IC₅₀ 31.42-88.03 μg/mL), while four displayed mild (IC₅₀> 100 μg/mL) antiplasmodial activity. The leaf ethyl acetate and methanol extracts of L. aspera; ethyl acetate, acetone, and methanol extracts of P. acidus; and seed acetone extract of T. chebula had good antiplasmodial activity (IC_so=7.81, 22.76, 9.37, 14.65, 12.68, and 4.76 μg/mL) with selectivity indices 5.43, 2.04, 4.88, 3.35, 3.42, and 9.97 for HEp-2 and >5.79, >2.20, >11.75, >3.41, >3.94, and >7.38 for Vero cells, respectively. These analyses have revealed for the first time that the components present in the solvent extracts of L. aspera, P. acidus, and T. chebula have antiplasmodial activity. The high antiplasmodial activity observed make these plants good candidates for isolation of anti-protozoal compounds which could serve as new lead structures for drug development.
C02	`01`	`X`		`@0 002A12A01`
C02	`02`	`X`		`@0 002B05E02B4`
C03	`01`	`X`	`FRE`	`@0 Activité @5 01`
C03	`01`	`X`	`ENG`	`@0 Activity @5 01`
C03	`01`	`X`	`SPA`	`@0 Actividad @5 01`
C03	`02`	`X`	`FRE`	`@0 Paludisme @5 02`
C03	`02`	`X`	`ENG`	`@0 Malaria @5 02`
C03	`02`	`X`	`SPA`	`@0 Paludismo @5 02`
C03	`03`	`X`	`FRE`	`@0 Parasite @5 03`
C03	`03`	`X`	`ENG`	`@0 Parasite @5 03`
C03	`03`	`X`	`SPA`	`@0 Parásito @5 03`
C03	`04`	`X`	`FRE`	`@0 Plasmodium falciparum @2 NS @5 55`
C03	`04`	`X`	`ENG`	`@0 Plasmodium falciparum @2 NS @5 55`
C03	`04`	`X`	`SPA`	`@0 Plasmodium falciparum @2 NS @5 55`
C07	`01`	`X`	`FRE`	`@0 Protozoose`
C07	`01`	`X`	`ENG`	`@0 Protozoal disease`
C07	`01`	`X`	`SPA`	`@0 Protozoosis`
C07	`02`	`X`	`FRE`	`@0 Parasitose`
C07	`02`	`X`	`ENG`	`@0 Parasitosis`
C07	`02`	`X`	`SPA`	`@0 Parasitosis`
C07	`03`	`X`	`FRE`	`@0 Infection`
C07	`03`	`X`	`ENG`	`@0 Infection`
C07	`03`	`X`	`SPA`	`@0 Infección`
C07	`04`	`X`	`FRE`	`@0 Apicomplexa @2 NS`
C07	`04`	`X`	`ENG`	`@0 Apicomplexa @2 NS`
C07	`04`	`X`	`SPA`	`@0 Apicomplexa @2 NS`
C07	`05`	`X`	`FRE`	`@0 Protozoa @2 NS`
C07	`05`	`X`	`ENG`	`@0 Protozoa @2 NS`
C07	`05`	`X`	`SPA`	`@0 Protozoa @2 NS`
N21				`@1 150`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

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Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Antiplasmodial activity of botanical extracts against Plasmodium falciparum</title>
<author><name sortKey="Bagavan, Asokan" sort="Bagavan, Asokan" uniqKey="Bagavan A" first="Asokan" last="Bagavan">Asokan Bagavan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509</s1>
<s2>Vellore District, Tamil Nadu</s2>
<s3>IND</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>Inde</country>
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<author><name sortKey="Abdul Abdul Rahuman" sort="Abdul Abdul Rahuman" uniqKey="Abdul Abdul Rahuman" last="Abdul Abdul Rahuman">ABDUL ABDUL RAHUMAN</name>
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<s2>Vellore District, Tamil Nadu</s2>
<s3>IND</s3>
<sZ>1 aut.</sZ>
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<author><name sortKey="Kamaraj, Chinnaperumal" sort="Kamaraj, Chinnaperumal" uniqKey="Kamaraj C" first="Chinnaperumal" last="Kamaraj">Chinnaperumal Kamaraj</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509</s1>
<s2>Vellore District, Tamil Nadu</s2>
<s3>IND</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
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<country>Inde</country>
</affiliation>
</author>
<author><name sortKey="Naveen Kumar Kaushik" sort="Naveen Kumar Kaushik" uniqKey="Naveen Kumar Kaushik" last="Naveen Kumar Kaushik">NAVEEN KUMAR KAUSHIK</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg</s1>
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<sZ>5 aut.</sZ>
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<author><name sortKey="Mohanakrishnan, Dinesh" sort="Mohanakrishnan, Dinesh" uniqKey="Mohanakrishnan D" first="Dinesh" last="Mohanakrishnan">Dinesh Mohanakrishnan</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg</s1>
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<author><name sortKey="Sahal, Dinkar" sort="Sahal, Dinkar" uniqKey="Sahal D" first="Dinkar" last="Sahal">Dinkar Sahal</name>
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<s2>New Delhi 110067</s2>
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<series><title level="j" type="main">Parasitology research : (1987)</title>
<title level="j" type="abbreviated">Parasitol. res. : (1987)</title>
<idno type="ISSN">0932-0113</idno>
<imprint><date when="2011">2011</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Activity</term>
<term>Malaria</term>
<term>Parasite</term>
<term>Plasmodium falciparum</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Activité</term>
<term>Paludisme</term>
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<front><div type="abstract" xml:lang="en">The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. The aim of the study was to discover effective plant extracts against Plasmodium falciparum. In the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Citrus sinensis (peel), Leucas aspera, Ocimum sanctum, Phyllanthus acidus (leaf), Terminalia chebula (seed) were tested for their antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of P. falciparum which was cultured following the candle-jar method. Antimalarial evaluations of daily replacement of culture medium containing CQ and different plant crude extracts were performed on 96-well plates at 37°C for 24 and 48 h. Parasitemia was determined microscopically on thin-film Giemsa-stained preparations. Plant extracts were tested for their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on human laryngeal cancer cell line (HEp-2) and normal cell line (Vero). Out of the 25 extracts tested, six showed good (IC<sub>so</sub>
 4.76-22.76 μg/mL), 15 exhibited moderate (IC<sub>50</sub>
 31.42-88.03 μg/mL), while four displayed mild (IC<sub>50</sub>
> 100 μg/mL) antiplasmodial activity. The leaf ethyl acetate and methanol extracts of L. aspera; ethyl acetate, acetone, and methanol extracts of P. acidus; and seed acetone extract of T. chebula had good antiplasmodial activity (IC<sub>so</sub>
=7.81, 22.76, 9.37, 14.65, 12.68, and 4.76 μg/mL) with selectivity indices 5.43, 2.04, 4.88, 3.35, 3.42, and 9.97 for HEp-2 and >5.79, >2.20, >11.75, >3.41, >3.94, and >7.38 for Vero cells, respectively. These analyses have revealed for the first time that the components present in the solvent extracts of L. aspera, P. acidus, and T. chebula have antiplasmodial activity. The high antiplasmodial activity observed make these plants good candidates for isolation of anti-protozoal compounds which could serve as new lead structures for drug development.</div>
</front>
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<fA02 i1="01"><s0>PARREZ</s0>
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<fA03 i2="1"><s0>Parasitol. res. : (1987)</s0>
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<fA05><s2>108</s2>
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<fA06><s2>5</s2>
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<fA08 i1="01" i2="1" l="ENG"><s1>Antiplasmodial activity of botanical extracts against Plasmodium falciparum</s1>
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<fA11 i1="01" i2="1"><s1>BAGAVAN (Asokan)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>ABDUL ABDUL RAHUMAN</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>KAMARAJ (Chinnaperumal)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>NAVEEN KUMAR KAUSHIK</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>MOHANAKRISHNAN (Dinesh)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>SAHAL (Dinkar)</s1>
</fA11>
<fA14 i1="01"><s1>Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509</s1>
<s2>Vellore District, Tamil Nadu</s2>
<s3>IND</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg</s1>
<s2>New Delhi 110067</s2>
<s3>IND</s3>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA20><s1>1099-1109</s1>
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<fA21><s1>2011</s1>
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</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>5859</s2>
<s5>354000191467460040</s5>
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<fA44><s0>0000</s0>
<s1>© 2011 INIST-CNRS. All rights reserved.</s1>
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<fA64 i1="01" i2="1"><s0>Parasitology research : (1987)</s0>
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<fA66 i1="01"><s0>DEU</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. The aim of the study was to discover effective plant extracts against Plasmodium falciparum. In the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of Citrus sinensis (peel), Leucas aspera, Ocimum sanctum, Phyllanthus acidus (leaf), Terminalia chebula (seed) were tested for their antimalarial activity against chloroquine (CQ)-sensitive (3D7) strain of P. falciparum which was cultured following the candle-jar method. Antimalarial evaluations of daily replacement of culture medium containing CQ and different plant crude extracts were performed on 96-well plates at 37°C for 24 and 48 h. Parasitemia was determined microscopically on thin-film Giemsa-stained preparations. Plant extracts were tested for their cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on human laryngeal cancer cell line (HEp-2) and normal cell line (Vero). Out of the 25 extracts tested, six showed good (IC<sub>so</sub>
 4.76-22.76 μg/mL), 15 exhibited moderate (IC<sub>50</sub>
 31.42-88.03 μg/mL), while four displayed mild (IC<sub>50</sub>
> 100 μg/mL) antiplasmodial activity. The leaf ethyl acetate and methanol extracts of L. aspera; ethyl acetate, acetone, and methanol extracts of P. acidus; and seed acetone extract of T. chebula had good antiplasmodial activity (IC<sub>so</sub>
=7.81, 22.76, 9.37, 14.65, 12.68, and 4.76 μg/mL) with selectivity indices 5.43, 2.04, 4.88, 3.35, 3.42, and 9.97 for HEp-2 and >5.79, >2.20, >11.75, >3.41, >3.94, and >7.38 for Vero cells, respectively. These analyses have revealed for the first time that the components present in the solvent extracts of L. aspera, P. acidus, and T. chebula have antiplasmodial activity. The high antiplasmodial activity observed make these plants good candidates for isolation of anti-protozoal compounds which could serve as new lead structures for drug development.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A12A01</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002B05E02B4</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Activité</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Activity</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Actividad</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Paludisme</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Malaria</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Paludismo</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Parasite</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Parasite</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Parásito</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Plasmodium falciparum</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Plasmodium falciparum</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Plasmodium falciparum</s0>
<s2>NS</s2>
<s5>55</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Protozoose</s0>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Protozoal disease</s0>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Protozoosis</s0>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Parasitose</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Parasitosis</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Parasitosis</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Apicomplexa</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Apicomplexa</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Apicomplexa</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Protozoa</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Protozoa</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Protozoa</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>150</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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Antiplasmodial activity of botanical extracts against Plasmodium falciparum

Antiplasmodial activity of botanical extracts against Plasmodium falciparum

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